Molecular Diagnostics Toolkit

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Enzymes are the heart of each and every diagnostic test. Enzymes are the means we can track, study and defeat old and new infectious diseases. However, enzymes are globally distributed from centralized foundries and, most often, require a cold chain for stability restricting their access to the many, even more in times of supply chain disruptions like is happening in the current COVID-19 pandemic.

The Molecular Diagnostics Toolkit includes:

IPTG inducible, T7 promoter driven ready-to-use expression cassettes to produce essential enzymes used in diagnostic among which RT, Bst-LF, RPA enzymes, RNA inhibitors. Enzymes are His-tagged and cassettes design are based on literature and experimental evidence.
A set of constructs for the production of Sars-CoV-2 RNA positive and negative controls;

What can it be used for:

The collection wants to provide researchers worldwide with an open source toolkit to produce their own enzymes, streamlining their research in diagnostics irrespective of supply chain disruptions.

This product is made available under the unilateral OpenMTA. Read Terms of Service at the bottom of the page for details.

Where can I find more information:

If you would like to design your own expression cassette, changing promoter, tag, reporter gene or terminator have a look at our Expression Toolkit collection.

Genes

Gene	Name	NCBI ID
HIV-RT	Human Immunodeficiency Virus Reverse Transcriptase
OMMLV	Moloney Murine Leukemia Virus Reverse Transcriptase
OpenVent	OpenVent DNA polymerase
RNAseInhib	RNAse Inhibitor
EcPPA Pyrophosphatase	Inorganic pyrophosphatase from E.coli
T7_Orf1A_1	SARS-CoV-2 Orf1A positive control	N/A
T7_Orf1A_2	SARS-CoV-2 Orf1A 2 positive control	N/A
T7_Orf1B_3	SARS-CoV-2 Orf1B positive control	N/A
T7_Orf1E_4	SARS-CoV-2 Orf1E positive control	N/A
T7_OrfN_5	SARS-CoV-2 OrfN positive control	N/A
Sars_NegativeControl	SARS-CoV-2 negative control	N/A

MTA

Unilateral OpenMTA

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Use: The Recipient shall use, store, and dispose of the Material and any Modifications in accordance with good laboratory practice and shall ensure compliance with all applicable laws, rules, and regulations, including laws, rules, and regulations governing export control and safety.
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Material: Original Material, Progeny, and Unmodified Derivatives. The Material shall not include: (a) Modifications, or (b) other substances created by the Recipient through the use of the Material, which are not Modifications, Progeny, or Unmodified Derivatives.
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