Enzymes are the heart of each and every diagnostic test. Enzymes are the means we can track, study and defeat old and new infectious diseases. However, enzymes are globally distributed from centralized foundries and, most often, require a cold chain for stability restricting their access to the many, even more in times of supply chain disruptions like is happening in the current COVID-19 pandemic.
The Molecular Diagnostics Toolkit includes:
- IPTG inducible, T7 promoter driven ready-to-use expression cassettes to produce essential enzymes used in diagnostic among which RT, Bst-LF, RPA enzymes, RNA inhibitors. Enzymes are His-tagged and cassettes design are based on literature and experimental evidence
- A set of constructs for the production of Sars-CoV-2 RNA positive and negative controls
What can it be used for:
The collection wants to provide researchers worldwide with an open source toolkit to produce their own enzymes, streamlining their research in diagnostics irrespective of supply chain disruptions.
The expression cassettes in this collection have been shown to work in literature. For distribution purposes we had to clone the newly synthesized cassettes in a novel off-patent vector backbone. The expression from this new vector has not been validated yet.
This product is made available under the unilateral OpenMTA.
Where can I find more information:
If you would like to design your own expression cassette, changing promoter, tag, reporter gene or terminator have a look at our Expression Toolkit collection.
|MERScontrol||MERS negative control||N/A|
|Pfu_Ssod7 HiFi||Pfu_Ssod7 HiFi - High Fidelity DNA polymerase|
|Ecoli_opt_RNAseInhib||E coli optimized RNAse Inhibitor|
|HIV-RT||Human Immunodeficiency Virus Reverse Transcriptase|
|OMMLV||Moloney Murine Leukemia Virus Reverse Transcriptase|
|OpenVent||OpenVent DNA polymerase|
|EcPPA Pyrophosphatase||Inorganic pyrophosphatase from E.coli|
|T7_Orf1A_1||SARS-CoV-2 Orf1A positive control||N/A|
|T7_Orf1A_2||SARS-CoV-2 Orf1A 2 positive control||N/A|
|T7_Orf1B_3||SARS-CoV-2 Orf1B positive control||N/A|
|T7_Orf1E_4||SARS-CoV-2 Orf1E positive control||N/A|
|T7_OrfN_5||SARS-CoV-2 OrfN positive control||N/A|
|Sars_NegativeControl||SARS-CoV-2 negative control||N/A|