Tropical Infectious Diseases Diagnostics and Therapeutics Research Toolkit

Tropical Infectious Diseases Diagnostics and Therapeutics Research Toolkit

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What is it?

Tropical infectious diseases present threats to tropical regions around the world; approximately 15 million people die every year as a result of infection from tropical infectious diseases and parasites. Research into diagnostic, therapeutic and vaccine development for these diseases are greatly needed, however expenses are limiting to researchers. We present this toolkit in support of fundamental tropical infectious disease research.

This toolkit contains S. cerevisiae codon-optimized genes encoding for envelope (E) and non-structural proteins (NS1) of major Flaviviruses, namely all four serotypes of Dengue, Zika and Japanese Encephalitis viruses (JEV). The encoded amino acid sequences have been selected to be the most prevalent variants of each type of virus. 

 

What can it be used for?

After being cloned into a yeast expression plasmid, these yeast optimized genes can be used to produce recombinant proteins in S. cerevisiae for a number of different purposes, including antigen, and/or antibody production. The antigens and antibodies can be used for basic research on viral pathogenesis, epitope mapping, and in developing novel diagnostic methods or novel vaccines against the Flaviviruses. They can also be used for educational purposes, such as demonstration of protein expression and purification in yeast, or engineering protein production through optimizing expression cassettes and/or expression conditions.

 

Where can I find more information?

The amino acid sequences can be found on NCBI Genpept using the following information:

Dengue virus 1 E protein (AQV12345.1 281-775)

Dengue virus 1 NS1 protein (AYP74685.1 776-1127)

Dengue virus 2 E protein (AYP74885.1 281-775)

Dengue virus 2 NS1 protein (AHG23160.1 776-1127)

Dengue virus 3 E protein (AFK83764.1 281-775)

Dengue virus 3 NS1 protein (AHG23254.1 774-1125)

Dengue virus 4 E protein (ACQ44401.1 280-774)

Dengue virus 4 NS1 protein (AFX65870.1 775-1126)

Zika virus E protein (AZS35375.1 291-794)

Zika virus NS1 protein (AAB18951.1 795-1146)

Japanese encephalitis virus E protein (AEO86789.1 295-794)

Japanese encephalitis virus N protein (AAB1895.1 795-1146)

 

The DNA sequences can be found on NCBI Nucleotide using the following information:

Yeast codon-optimized Dengue virus 1 Envelope glycoprotein (MW324565)

https://www.ncbi.nlm.nih.gov/nuccore/MW324565

Yeast codon-optimized Dengue virus 1 Non-structural protein 1 (MW324566)

https://www.ncbi.nlm.nih.gov/nuccore/MW324566

Yeast codon-optimized Dengue virus 2 Envelope glycoprotein (MW324567)

https://www.ncbi.nlm.nih.gov/nuccore/MW324567

Yeast codon-optimized Dengue virus 2 Non-structural protein 1 (MW324568)

https://www.ncbi.nlm.nih.gov/nuccore/MW324568

Yeast codon-optimized Dengue virus 3 Envelope glycoprotein (MW324569)

https://www.ncbi.nlm.nih.gov/nuccore/MW324569

Yeast codon-optimized Dengue virus 3 Non-structural protein 1 (MW324570)

https://www.ncbi.nlm.nih.gov/nuccore/MW324570

Yeast codon-optimized Dengue virus 4 Envelope glycoprotein (MW324571)

https://www.ncbi.nlm.nih.gov/nuccore/MW324571

Yeast codon-optimized Dengue virus 4 Nonstructural protein (MW324573)

https://www.ncbi.nlm.nih.gov/nuccore/MW324573

Yeast codon-optimized Zika virus Envelope glycoprotein (MW324573)

https://www.ncbi.nlm.nih.gov/nuccore/MW324573

Yeast codon-optimized Zika virus Non-structural protein 1 (MW324574)

https://www.ncbi.nlm.nih.gov/nuccore/MW324574

Yeast codon-optimized Japanese Encephalitis Virus Envelope glycoprotein (MW324575)

https://www.ncbi.nlm.nih.gov/nuccore/MW324575

Yeast codon-optimized Japanese Encephalitis Virus Non-structural glycoprotein (MW324576)

https://www.ncbi.nlm.nih.gov/nuccore/MW324576

 

Image attributed to Cornell University – PJ Mode Collection of Persuasive Cartography

Genes

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