The Open Enzyme Collection consists of 81 genes for enzymes that are "workhorses" of molecular biology, including DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases and restriction enzymes. The enzymes are useful in both basic and applied fields as diverse as genomics, diagnostics, biodiversity, synthetic biology, DNA origami, immunology, biochemistry and more.
The foundational technologies enabled by this collection of include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and dNTP synthesis. Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve functionality.
The Open Bioeconomy Lab designed and codon-optimized the Open Enzymes parts for expression in Escherichia coli bacteria. All enzymes are obtained from expired patents or for various reasons are not encumbered by patent rights.
What can the collection be used for?
Researchers can use the collection as the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice and then transforming into E. coli bacteria. This can help reduce lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder to obtain. The enzymes can also be used as a base to improve functionality or add new functions through genetic engineering and directed evolution.
Open Enzymes are not provided in expression vectors. In order to produce an enzyme, you must subclone your gene of interest into an expression vector. Open Enzymes are also compatible with the E. coli Protein Expression Toolkit (all parts have CE overhangs). More information about the collection can be obtained from the Open Bioeconomy Lab.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
Instructions for Use:
Open Enzymes is Shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
Transform into Top10 E. coli or equivalent. Pick a colony and culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.
You may find an expression guide on the Open Bioeconomy Lab website.
The current KOD polymerase gene (Well F1) produces a nonfunctional protein. We are working to replace the polymerase gene with a functional version. Thank you to Tomas Moravec for pointing out the issue!
If you have already received the collection, check out the plate layout.
1 Plate in Set
This kit is included in kit plate 2 of the 2022 iGEM distribution. The plate map for kit plate 2 of the iGEM 2022 distribution can be found here.
Prior Kit Versions:
If your plate is labeled "Open Enzymes Part 1" or "openenzymes_1," you can find a plate map here.
If your plate is labeled "Open Enzymes Part 2," you can find a plate map here.
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| 9N7polA | 9°N-7 DNA polymerase | BBF10K_003247 |
| K12polLF | DNA Polymerase I, Large (Klenow) Fragment | BBF10K_003248 |
| K12polLF (exo) | DNA Polymerase I, Large (Klenow) Fragment (3′→5′ exo-) | BBF10K_003249 |
| ECOpolA | E. coli DNA Polymerase I | BBF10K_003250 |
| KTHEAQpol | KlenTaq1 polymerase | BBF10K_003251 |
| KODpol | KOD DNA polymerase | BBF10K_003252 |
| Pwopol | Pwo DNA Polymerase | BBF10K_003254 |
| GBDpol (exo) | Pyrococcus Sp. Heat-Stable (exo–) DNA Polymerase (Deep Vent™ (exo–) DNA Polymerase at NEB) | BBF10K_003255 |
| GBDpol | Pyrococcus Sp. Heat-Stable DNA Polymerase (Deep Vent™ at NEB) | BBF10K_003256 |
| THEAQpolA | Taq DNA Polymerase | BBF10K_003257 |
| Tlipol (exo) | Tli DNA polymerase (exo-) (available from NEB as VentR® (exo-) DNA Polymerase) | BBF10K_003259 |
| TthpolA | Tth DNA polymerase | BBF10K_003260 |
| Bstpol | Bst DNA Polymerase, Full Length | BBF10K_003261 |
| BstpolLF | Bst DNA Polymerase, Large Fragment | BBF10K_003262 |
| BsupolLF | Bsu DNA Polymerase I, Large Fragment | BBF10K_003263 |
| phi29pol | phi29 DNA Polymerase | BBF10K_003264 |
| 9N7polA (CT) | 9°N-7 DNA Polymerase chain terminating (NEB > Therminator DNA Pol) | BBF10K_003265 |
| dbh | Sulfolobus DNA Polymerase IV | BBF10K_003266 |
| T4gene43 | T4 DNA Polymerase | BBF10K_003267 |
| T7gene5 | T7 DNA Polymerase (unmodified) | BBF10K_003268 |
| T5gene122 | T5 DNA Polymerase | BBF10K_003269 |
| T4gene30 | T4-DNA Ligase | BBF10K_003270 |
| ECOligA | E. coli DNA Ligase | BBF10K_003271 |
| HSlig3 | DNA Lligase III | BBF10K_003272 |
| HSlig4 | DNA Ligase IV | BBF10K_003273 |
| 9N7lig | 9 Degrees North DNA Ligase | BBF10K_003274 |
| Pfulig | Pfu DNA Ligase | BBF10K_003275 |
| THEAQlig | Taq DNA Ligase | BBF10K_003276 |
| T4gene63 | T4 RNA Ligase 1 | BBF10K_003277 |
| ALPI | CIP (calf intestinal phosphatase) | BBF10K_003278 |
| RNAse A | RNAse A | BBF10K_003279 |
| RNAse H | RNAse H | BBF10K_003280 |
| EcoRIR | EcoRI restriction enzyme | BBF10K_003281 |
| EcoRIM | EcoRI methyltransferase | BBF10K_003282 |
| PstIR | PstI restriction enzyme | BBF10K_003283 |
| PstIM | PstI methyltransferase | BBF10K_003284 |
| MMLV_RT (mut H) | Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations) | BBF10K_003285 |
| MMLV_RT (lack H) | Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) (SuperScriptII) | BBF10K_003286 |
| TthRT | Thermus thermophilus (Tth) RT | BBF10K_003287 |
| T3RNAp | T3 RNA Polymerase | BBF10K_003289 |
| T7RNAp | T7 RNA Polymerase | BBF10K_003290 |
| SP6RNAp | SP6 RNA Polymerase | BBF10K_003291 |
| Eco31I | Eco31I restriction enzyme | BBF10K_003292 |
| Eco31IA | Eco31IA methyltransferase | BBF10K_003293 |
| Eco31IB | Eco31IB methyltransferase | BBF10K_003294 |
| SapIR | SapI restriction enzyme | BBF10K_003295 |
| SapIM1 | M1.SapI methyltransferase | BBF10K_003296 |
| SapIM2 | M2.SapI methyltransferase | BBF10K_003297 |
| XbaIR | XbaI restriction enzyme | BBF10K_003298 |
| XbaIM | M.XbaI methyltransferase | BBF10K_003299 |
| NotIR (GC) | NotI restriction enzyme | BBF10K_003300 |
| NotIM | M.NotI methyltransferase | BBF10K_003301 |
| SfiIR (cDNA) | SfiI (used to create cDNA libraries) | BBF10K_003302 |
| SfiIM | M.SfiI methyltransferase | BBF10K_003303 |
| EcoRV | EcoRV restriction enzyme | BBF10K_003304 |
| EcoRVM | M.EcoRV methyltransferase | BBF10K_003305 |
| NcoIR | NcoI restriction enzyme | BBF10K_003306 |
| NcoIM | M.NcoI methyltransferase | BBF10K_003307 |
| HindIIIR | HindIII restriction enzyme | BBF10K_003308 |
| HindIIIM | M.HindIII methyltransferase | BBF10K_003309 |
| DpnIR | DpnI restriction enzyme | BBF10K_003310 |
| Bst HF | Bst polymerase, High fidelity | BBF10K_003311 |
| ETSSB | Extreme Thermostable Single-Stranded DNA Binding Protein | BBF10K_003312 |
| T5gene12/13 | T5 Exonuclease | BBF10K_003313 |
| TthligK294R | Tth DNA Ligase K294R | BBF10K_003314 |
| dromedNK | dNK from drosophila | BBF10K_003315 |
| TEV protease | TEV protease | BBF10K_003316 |
| T4 PNK | T4 Polynucleotide Kinase | BBF10K_003317 |
| T4-BGT | T4 Phage β-glucosyltransferase | BBF10K_003318 |
| T7EI | T7EI (T7 Endonuclease I) | BBF10K_003319 |
| T4 PGD | T4 PGD (T4 Endonuclease V) | BBF10K_003320 |
| T4 g32 | T4 gene 32 | BBF10K_003321 |
| P45 | P45 (ArchaeMaxx/PfuTurbo) | BBF10K_003322 |
| BovDNTT | Bovine DNTT | BBF10K_003323 |
| DNA Topoisomerase 1B Vaccinia Virus | DNA Topoisomerase 1B Vaccinia Virus | BBF10K_003324 |
| lambda red operon | lambda red operon (exo, bet, gam) | BBF10K_003325 |
| UDG | Uracil DNA glycosylase | BBF10K_003326 |
| Fpg | Formamidopyrimidine DNA Glycosylase | BBF10K_003327 |
| Endonuclease IV E. coli | Endonuclease IV E. coli | BBF10K_003328 |
| Endonuclease VIII E. coli | Endonuclease VIII E. coli | BBF10K_003329 |
| Exonuclease III E. coli | Exonuclease III E. coli | BBF10K_003330 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Jianxiang Shi | jianxiangshi {at} zzu {dot} edu {dot} cn | China |
| Maira Goytia | mgoytia {at} spelman {dot} edu | United States |
| Alexandr Dorif | dorif11 {at} gmail {dot} com | Moldova, Republic of |
| Kheng Oon Low | khengoon {at} nibm {dot} my | Malaysia |
| Juan Sebastian Alvarez | juan.alvarez1 {at} ucalgary {dot} ca | Canada |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Muhammad Saad Rehmani | saadrehmani88 {at} hotmail {dot} com | China |
| Maan Neamah | maanm.neamah {at} uokufa {dot} edu {dot} iq | Iraq |
| elwi machado | elwi.machado {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| Benjamin Loang | benjamin.liang {at} uq {dot} edu {dot} au | Australia |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Nicholas White | nickk.white {at} gmail {dot} com | United States |
| Arthur Zanetti Nunes Fernandes | fgueiros {at} iq {dot} usp {dot} br | United States |
| Marko Komlosh | markokomlosh {at} gmail {dot} com | Serbia |
| Kristel Alman | kristel.alman {at} ut {dot} ee | Estonia |
| Stijn Tobias de Vries | stijn.vries {at} mpi-marburg {dot} mpg {dot} de | Germany |
| Jennifer Hsieh | j.hsieh {at} kurabiotech {dot} com | Chile |
| Josephine Labos | N/A | Philippines |
| Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Fernando Bustos | fernando.bustos {at} genosur {dot} com | Chile |
| Hamidreza Karbalaeiheidari | karbalaei76 {at} gmail {dot} com | Canada |
| Keith Gagnon | ktgagnon {at} gmail {dot} com | United States |
| Rodolfo Marcolino | rodolfo.marcolino {at} me {dot} com | Argentina |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Jehovani Lopez | jlopez {at} genspace {dot} org | United States |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Romain Volmer | romain.volmer {at} envt {dot} fr | France |
| Codruta Ignea | codruta.ignea {at} mcgill {dot} ca | Canada |
| Andrei Berasneu | a.berasneu {at} kleverlab {dot} pl | Poland |
| N Sadananda Singh | nsslabresearch {at} gmail {dot} com | India |
| Phil Jensik | NA | United States |
| Bronagh Hogan | wolfgang.fischle {at} kaust {dot} edu {dot} sa | Saudi Arabia |
| Sarah Ware | sarah.ware {at} bioblaze {dot} org | United States |
| Joel Graff | jgraff {at} mtech {dot} edu | United States |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Franklin Nobrega | f.nobrega {at} soton {dot} ac {dot} uk | United Kingdom |
| Muhammad Shoaib | mshoaib {at} lums {dot} edu {dot} pk | Pakistan |
| Bunpote Siridechadilok | bunpote.sir {at} biotec {dot} or {dot} th | Thailand |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Joey Riepsaame | joey.riepsaame {at} path {dot} ox {dot} ac {dot} uk | United Kingdom |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Dayananda Chandrappa | e2ebiotech {at} gmail {dot} com | India |
| Alex Nguyen | alex.nguyenba {at} utoronto {dot} ca | Canada |
| Tri Ngo | NA | United States |
| Norhan Hassan | norhan.hassan {at} kaust {dot} edu {dot} sa | Saudi Arabia |
| Deepak Saini | deepaksaini {at} iisc {dot} ac {dot} in | India |
| William Fried | uscigem {at} usc {dot} edu | United States |
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
| Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Joao Vitor Dutra Molino | candidomolino {at} gmail {dot} com | United States |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Ingrid Lorena Jaramillo | Lojadel2 {at} gmail {dot} com | Colombia |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | United States |
| Dayananda Chandrappa | nanda.daya {at} gmail {dot} com | India |
| Julio Bonilla | jabonill {at} espol {dot} edu {dot} ec | Ecuador |
| Nikolaus Dietz | nikolaus.dietz {at} unibas {dot} ch | Switzerland |
| Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
| Andrei Berasneu | andreyberesnevson {at} gmail {dot} com | Poland |
| Kelly Gallagher | kelly.gallagher {at} oneonta {dot} edu | United States |
| Matthew Knuesel | mknuesel {at} hudsonalpha {dot} org | United States |
| Umair Hanif | umair.hanif {at} gdec {dot} edu {dot} pk | Pakistan |
| Chris Badenhorst | chris.badenhorst {at} uni-greifswald {dot} de | Germany |
| Vincent Paris | NA | United States |
| Danielle Pedrolli | danielle.pedrolli {at} unesp {dot} br | Brazil |
| Marc Juul | marc {at} juul {dot} io | United States |
| Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Ashton Cropp | tacropp {at} vcu {dot} edu | United States |
| Tocvic Meng | Tocvic Meng \ Email: tocvic.meng {at} holocyte {dot} com \ website https://gmexpression {dot} com/ | Australia |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Haoyong WANG | research | China |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
| Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
| Moritz Venne | moritz.venne {at} web {dot} de | Germany |
| Wapouo Fadanka Stephane | mboalab {at} gmail {dot} com | Republic of Cameroon |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Florian Leiß-Maier | florian.leiss-maier {at} tum {dot} de | Germany |
| Intan Taufik | i.taufik {at} sith {dot} itb {dot} ac {dot} id | Indonesia |
| Cole LaBonne | colelabonne {at} hotmail {dot} com | United States |
| Anil Challa | akchalla {at} uab {dot} edu | United States |
| Adrian Filips | tada {at} specyal {dot} com | Canada |
| James Altamirano | James_altam {at} utexas {dot} edu | United States |
| Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| mehmet tardu | mtardu {at} gmail {dot} com | Turkey |
| Dong Nguyen Tam | NA | Vietnam |
| Matthew Thompson | matthew.thompson-12 {at} postgrad {dot} manchester {dot} ac {dot} uk | United Kingdom |
| Kulvinder Gill | kanwar {at} geneshifters {dot} com | United States |
| Zhiyong Lei | zlei {at} umcutrecht {dot} nl | Netherlands |
| Tal Globus | bio {at} talglobus {dot} com | United States |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Paulina Ejsmont | research.lab {at} cri-paris {dot} org | France |
| Shobha KrupaRani | pshobha {at} ccmb {dot} res {dot} in | India |
| Watanyu Bunsermyos | watanyu.bu {at} ku {dot} th | Thailand |
| Emin Bursa | NA | United States |
| Elizabeth Bilsland | bilsland {at} unicamp {dot} br | Brazil |
| Jonathan Jonathan | Jonathancontact16 {at} gmail {dot} com | Indonesia |
| Bingbing Wan | wanb {at} sjtu {dot} edu {dot} cn | China |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| Ben Davis | bed58 {at} aber {dot} ac {dot} uk | United Kingdom |
| Stephen Klusza | StephenKlusza {at} clayton {dot} edu | United States |
| Muzaffar Muminov | muminov.imbio {at} gmail {dot} com | Uzbekistan |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | United States |
| Jorge Marchand | jmarcha {at} uw {dot} edu | United States |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Sarah Turcotte | turcottes {at} cbsnews {dot} com | United States |
| Gülşen Günel | gulsen.gunel {at} ogr {dot} iu {dot} edu {dot} tr | Turkey |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| Long Nguyen | Lnguyen2 {at} ufl {dot} edu | United States |
| Richard Jones | rpj1234rpj {at} gmail {dot} com | United States |
| Matias Cabeza | matiascabeza {at} gmail {dot} com | Argentina |
| Meng Lu | menglu {at} iastate {dot} edu | United States |
| Jonathan LeCureux | jlecureu {at} svsuedu | United States |
| Leandro Oliveira (NG) | ll637 {at} cam {dot} ac {dot} uk | United Kingdom |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| Simon Hofer | soyouwantalab {at} gmail {dot} com | Austria |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
| Ellen Jorgensen | ejorgensen {at} biotechwithoutborders {dot} org | United States |
| semih cagan | semihcgn {at} gmail {dot} com | Turkey |
| Thooyavan George | www.hetrogenebiotech.com | India |
| Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
| Friede van der Berg | 21555230 {at} sun {dot} ac {dot} za | South Africa |
| Durga Burande | burandedurga22 {at} gmail {dot} com | India |
| Michael Powers | mpowers9304 {at} gmail {dot} com | United States |
| Shawn Lyons | smlyons1 {at} bu {dot} edu | United States |
| David Truong | www.truonglab.com | United States |
| Ferdinand Molnár | NA | Kazakhstan |
| Andrew Gray | andrewg {at} bioq {dot} org {dot} au | Australia |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Yi Zhang | www.pharmozyme.com | United States |
| Vitaliy Strochkov | NA | Kazakhstan |
| Ivan Slovokhotov | ivan_slovohotov {at} mail {dot} ru | Russia |
Download all of this information as a CSV from our GitHub.
Product Comments/Discussions
Based on 7 reviews
Join In