The Open Enzyme Collection (Part 1) consists of 42 useful enzymes that are "work"horses" of molecular biology and are commonly used in labs for techniques involving detecting, altering and manipulating DNA. The enzymes are useful in both basic and applied fields as diverse as genomics, diagnostics, biodiversity, synthetic biology, DNA origami, immunology, biochemistry and more. The foundational technologies enabled by this collection of DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases and restriction enzymes includes polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA and other methods on which the field of molecular biology has been built.
The collection is codon-optimised for expression in Escherichia coli bacteria and all enzymes are obtained from expired patents or for various reasons are not encumbered by patent rights.
What can the collection be used for?
Researchers can use the collection as the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice and then transforming into E. coli bacteria. This can help reduce lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder to obtain. The enzymes can also be used as a base to improve functionality or add new functions through genetic engineering and directed evolution.
More information about the collection can be obtained from the Open Bioeconomy Lab.
This product is made available under the unilateral OpenMTA. Read Terms of Service at the bottom of the page for details.
Instructions for Use:
Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.
|9N7polA||9°N-7 DNA polymerase|
|K12polLF||DNA Polymerase I, Large (Klenow) Fragment|
|K12polLF (exo)||DNA Polymerase I, Large (Klenow) Fragment (3′→5′ exo-)|
|ECOpolA||E. coli DNA Polymerase I|
|KODpol||KOD DNA polymerase|
|Pwopol||Pwo DNA Polymerase|
|GBDpol (exo)||Pyrococcus Sp. Heat-Stable (exo–) DNA Polymerase (Deep Vent™ (exo–) DNA Polymerase at NEB)|
|GBDpol||Pyrococcus Sp. Heat-Stable DNA Polymerase (Deep Vent™ at NEB)|
|THEAQpolA||Taq DNA Polymerase|
|Tlipol (exo)||Tli DNA polymerase (exo-) (available from NEB as VentR® (exo-) DNA Polymerase)|
|TthpolA||Tth DNA polymerase|
|Bstpol||Bst DNA Polymerase, Full Length|
|BstpolLF||Bst DNA Polymerase, Large Fragment|
|BsupolLF||Bsu DNA Polymerase I, Large Fragment|
|phi29pol||phi29 DNA Polymerase|
|9N7polA (CT)||9°N-7 DNA Polymerase chain terminating (NEB > Therminator DNA Pol)|
|dbh||Sulfolobus DNA Polymerase IV|
|T4gene43||T4 DNA Polymerase|
|T7gene5||T7 DNA Polymerase (unmodified)|
|T5gene122||T5 DNA Polymerase|
|ECOligA||E. coli DNA Ligase|
|HSlig3||DNA Lligase III|
|HSlig4||DNA Ligase IV|
|9N7lig||9 Degrees North DNA Ligase|
|Pfulig||Pfu DNA Ligase|
|THEAQlig||Taq DNA Ligase|
|T4gene63||T4 RNA Ligase 1|
|ALPI||CIP (calf intestinal phosphatase)|
|RNAse A||RNAse A|
|RNAse H||RNAse H|
|EcoRIR||EcoRI restriction enzyme|
|PstIR||PstI restriction enzyme|
|MMLV_RT (mut H)||Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)|
|MMLV_RT (lack H)||Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) (SuperScriptII)|
|TthRT||Thermus thermophilus (Tth) RT|
|T3RNAp||T3 RNA Polymerase|
|T7RNAp||T7 RNA Polymerase|
|SP6RNAp||SP6 RNA Polymerase|