Open Enzymes

Open Enzymes

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The Open Enzyme Collection consists of 81 useful genes for enzymes that are "workhorses" of molecular biology, including DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases and restriction enzymes. The enzymes are useful in both basic and applied fields as diverse as genomics, diagnostics, biodiversity, synthetic biology, DNA origami, immunology, biochemistry and more.

The foundational technologies enabled by this collection of include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and dNTP synthesis. Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve functionality.

The collection is codon-optimised for expression in Escherichia coli bacteria and all enzymes are obtained from expired patents or for various reasons are not encumbered by patent rights.

What can the collection be used for?   
Researchers can use the collection as the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice and then transforming into E. coli bacteria. This can help reduce lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder to obtain. The enzymes can also be used as a base to improve functionality or add new functions through genetic engineering and directed evolution.

Open Enzymes are not provided in expression vectors. In order to produce an enzyme, you must subclone your gene of interest into an expression vector. Open Enzymes are also compatible with the E. coli  Protein Expression Toolkit (all parts have CE overhangs). More information about the collection can be obtained from the Open Bioeconomy Lab.

This product is made available under the unilateral OpenMTA

All genes are present on the plate in E. coli glycerol stocks. Some or all of these items are for use only as permitted by a research exemption.


Instructions for Use:

Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.

If you have already received the collection, check out the plate layout.

1 Plate in Set


Prior Kit Versions:

If your plate is labeled "Open Enzymes Part 1" or "openenzymes_1," you can find a plate map here.

If your plate is labeled "Open Enzymes Part 2," you can find a plate map here.



Gene Name
9N7polA 9°N-7 DNA polymerase
K12polLF DNA Polymerase I, Large (Klenow) Fragment
K12polLF (exo) DNA Polymerase I, Large (Klenow) Fragment (3′→5′ exo-)
ECOpolA E. coli DNA Polymerase I
KTHEAQpol KlenTaq1 polymerase
KODpol KOD DNA polymerase
Pwopol Pwo DNA Polymerase
GBDpol (exo) Pyrococcus Sp. Heat-Stable (exo–) DNA Polymerase (Deep Vent™ (exo–) DNA Polymerase at NEB)
GBDpol Pyrococcus Sp. Heat-Stable DNA Polymerase (Deep Vent™ at NEB)
THEAQpolA Taq DNA Polymerase
Tlipol (exo) Tli DNA polymerase (exo-) (available from NEB as VentR® (exo-) DNA Polymerase)
TthpolA Tth DNA polymerase
Bstpol Bst DNA Polymerase, Full Length
BstpolLF Bst DNA Polymerase, Large Fragment
BsupolLF Bsu DNA Polymerase I, Large Fragment
phi29pol phi29 DNA Polymerase
9N7polA (CT) 9°N-7 DNA Polymerase chain terminating (NEB > Therminator DNA Pol)
dbh Sulfolobus DNA Polymerase IV
T4gene43 T4 DNA Polymerase
T7gene5 T7 DNA Polymerase (unmodified)
T5gene122 T5 DNA Polymerase
T4gene30 T4-DNA Ligase
ECOligA E. coli DNA Ligase
HSlig3 DNA Lligase III
HSlig4 DNA Ligase IV
9N7lig 9 Degrees North DNA Ligase
Pfulig Pfu DNA Ligase
THEAQlig Taq DNA Ligase
T4gene63 T4 RNA Ligase 1
ALPI CIP (calf intestinal phosphatase)
EcoRIR EcoRI restriction enzyme
EcoRIM EcoRI methyltransferase
PstIR PstI restriction enzyme
PstIM PstI methyltransferase
MMLV_RT (mut H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
MMLV_RT (lack H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) (SuperScriptII)
TthRT Thermus thermophilus (Tth) RT
T3RNAp T3 RNA Polymerase
T7RNAp T7 RNA Polymerase
SP6RNAp SP6 RNA Polymerase
Eco31I Eco31I restriction enzyme
Eco31IA Eco31IA methyltransferase
Eco31IB Eco31IB methyltransferase
SapIR SapI restriction enzyme
SapIM1 M1.SapI methyltransferase
SapIM2 M2.SapI methyltransferase
XbaIR XbaI restriction enzyme
XbaIM M.XbaI methyltransferase
NotIR (GC) NotI restriction enzyme
NotIM M.NotI methyltransferase
SfiIR (cDNA) SfiI (used to create cDNA libraries)
SfiIM M.SfiI methyltransferase
EcoRV EcoRV restriction enzyme
EcoRVM M.EcoRV methyltransferase
NcoIR NcoI restriction enzyme
NcoIM M.NcoI methyltransferase
HindIIIR HindIII restriction enzyme
HindIIIM M.HindIII methyltransferase
DpnIR DpnI restriction enzyme
Bst HF Bst polymerase, High fidelity
ETSSB Extreme Thermostable Single-Stranded DNA Binding Protein
T5gene12/13 T5 Exonuclease
TthligK294R Tth DNA Ligase K294R
dromedNK dNK from drosophila
TEV protease TEV protease
T4 PNK T4 Polynucleotide Kinase
T4-BGT T4 Phage β-glucosyltransferase
T7EI T7EI (T7 Endonuclease I)
T4 PGD T4 PGD (T4 Endonuclease V)
T4 g32 T4 gene 32
P45 P45 (ArchaeMaxx/PfuTurbo)
DNA Topoisomerase 1B Vaccinia Virus DNA Topoisomerase 1B Vaccinia Virus
lambda red operon lambda red operon (exo, bet, gam)
UDG Uracil DNA glycosylase
Fpg Formamidopyrimidine DNA Glycosylase
Endonuclease IV E. coli Endonuclease IV E. coli
Endonuclease VIII E. coli Endonuclease VIII E. coli
Exonuclease III E. coli Exonuclease III E. coli


The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact
Vitaliy Strochkov NA
Amitabha Majumdar Mamitava {at} nccs {dot} res {dot} in
Dong Nguyen Tam NA
Matthew Phanchana matthew.pha {at} mahidol {dot} edu
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org
semih cagan semihcgn {at} gmail {dot} com

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