Open Enzymes (Part 1)

Open Enzymes (Part 1)

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The Open Enzyme Collection (Part 1) consists of 42 useful enzymes that are "work"horses" of molecular biology and are commonly used in labs for techniques involving detecting, altering and manipulating DNA. The enzymes are useful in both basic and applied fields as diverse as genomics, diagnostics, biodiversity, synthetic biology, DNA origami, immunology, biochemistry and more. The foundational technologies enabled by this collection of DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases and restriction enzymes includes polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA and other methods on which the field of molecular biology has been built.

The collection is codon-optimised for expression in Escherichia coli bacteria and all enzymes are obtained from expired patents or for various reasons are not encumbered by patent rights.

What can the collection be used for?
Researchers can use the collection as the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice and then transforming into E. coli bacteria. This can help reduce lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder to obtain. The enzymes can also be used as a base to improve functionality or add new functions through genetic engineering and directed evolution.

More information about the collection can be obtained from the Open Bioeconomy Lab.

This product is made available under the unilateral OpenMTA. Read Terms of Service at the bottom of the page for details.


Plate Layout

Instructions for Use:

Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.

PlateSet: OpenEnzyme

Genes

Gene Name
9N7polA 9°N-7 DNA polymerase
K12polLF DNA Polymerase I, Large (Klenow) Fragment
K12polLF (exo) DNA Polymerase I, Large (Klenow) Fragment (3′→5′ exo-)
ECOpolA E. coli DNA Polymerase I
KTHEAQpol KlenTaq1 polymerase
KODpol KOD DNA polymerase
Pwopol Pwo DNA Polymerase
GBDpol (exo) Pyrococcus Sp. Heat-Stable (exo–) DNA Polymerase (Deep Vent™ (exo–) DNA Polymerase at NEB)
GBDpol Pyrococcus Sp. Heat-Stable DNA Polymerase (Deep Vent™ at NEB)
THEAQpolA Taq DNA Polymerase
Tlipol (exo) Tli DNA polymerase (exo-) (available from NEB as VentR® (exo-) DNA Polymerase)
TthpolA Tth DNA polymerase
Bstpol Bst DNA Polymerase, Full Length
BstpolLF Bst DNA Polymerase, Large Fragment
BsupolLF Bsu DNA Polymerase I, Large Fragment
phi29pol phi29 DNA Polymerase
9N7polA (CT) 9°N-7 DNA Polymerase chain terminating (NEB > Therminator DNA Pol)
dbh Sulfolobus DNA Polymerase IV
T4gene43 T4 DNA Polymerase
T7gene5 T7 DNA Polymerase (unmodified)
T5gene122 T5 DNA Polymerase
T4gene30 T4-DNA Ligase
ECOligA E. coli DNA Ligase
HSlig3 DNA Lligase III
HSlig4 DNA Ligase IV
9N7lig 9 Degrees North DNA Ligase
Pfulig Pfu DNA Ligase
THEAQlig Taq DNA Ligase
T4gene63 T4 RNA Ligase 1
ALPI CIP (calf intestinal phosphatase)
RNAse A RNAse A
RNAse H RNAse H
EcoRIR EcoRI restriction enzyme
EcoRIM EcoRI methyltransferase
PstIR PstI restriction enzyme
PstIM PstI methyltransferase
MMLV_RT (mut H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
MMLV_RT (lack H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) (SuperScriptII)
TthRT Thermus thermophilus (Tth) RT
T3RNAp T3 RNA Polymerase
T7RNAp T7 RNA Polymerase
SP6RNAp SP6 RNA Polymerase


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