Open Enzymes (Part 1)

Open Enzymes (Part 1)

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The Open Enzyme Collection (Part 1) consists of 42 useful enzymes that are "work"horses" of molecular biology and are commonly used in labs for techniques involving detecting, altering and manipulating DNA. The enzymes are useful in both basic and applied fields as diverse as genomics, diagnostics, biodiversity, synthetic biology, DNA origami, immunology, biochemistry and more. The foundational technologies enabled by this collection of DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases and restriction enzymes includes polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA and other methods on which the field of molecular biology has been built.

The collection is codon-optimised for expression in Escherichia coli bacteria and all enzymes are obtained from expired patents or for various reasons are not encumbered by patent rights.

What can the collection be used for?
Researchers can use the collection as the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice and then transforming into E. coli bacteria. This can help reduce lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder to obtain. The enzymes can also be used as a base to improve functionality or add new functions through genetic engineering and directed evolution.

More information about the collection can be obtained from the Open Bioeconomy Lab.

This product is made available under the unilateral OpenMTA. Read Terms of Service at the bottom of the page for details.


Plate Layout

Instructions for Use:

Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.

PlateSet: OpenEnzyme

Genes

Gene Name NCBI ID
9°N-7 DNA polymerase 9°N-7 DNA polymerase
DNA Polymerase I, Large (Klenow) Fragment DNA Polymerase I, Large (Klenow) Fragment
DNA Polymerase I, Large (Klenow) Fragment (3′→5′ exo-) DNA Polymerase I, Large (Klenow) Fragment (3′→5′ exo-)
E. coli DNA Polymerase I E. coli DNA Polymerase I
KlenTaq1 polymerase KlenTaq1 polymerase
KOD DNA polymerase KOD DNA polymerase
Pwo DNA Polymerase Pwo DNA Polymerase
Pyrococcus Sp. Heat-Stable (exo–) DNA Polymerase (Deep Vent™ (exo–) DNA Polymerase at NEB) Pyrococcus Sp. Heat-Stable (exo–) DNA Polymerase (Deep Vent™ (exo–) DNA Polymerase at NEB)
Pyrococcus Sp. Heat-Stable DNA Polymerase (Deep Vent™ at NEB) Pyrococcus Sp. Heat-Stable DNA Polymerase (Deep Vent™ at NEB)
Taq DNA Polymerase Taq DNA Polymerase
Tli DNA polymerase (exo-) (available from NEB as VentR® (exo-) DNA Polymerase) Tli DNA polymerase (exo-) (available from NEB as VentR® (exo-) DNA Polymerase)
Tth DNA polymerase Tth DNA polymerase
Bst DNA Polymerase, Full Length Bst DNA Polymerase, Full Length
Bst DNA Polymerase, Large Fragment Bst DNA Polymerase, Large Fragment
Bsu DNA Polymerase I, Large Fragment Bsu DNA Polymerase I, Large Fragment
phi29 DNA Polymerase phi29 DNA Polymerase
9°N-7 DNA Polymerase chain terminating (NEB > Therminator DNA Pol) 9°N-7 DNA Polymerase chain terminating (NEB > Therminator DNA Pol)
Sulfolobus DNA Polymerase IV Sulfolobus DNA Polymerase IV
T4 DNA Polymerase T4 DNA Polymerase
T7 DNA Polymerase (unmodified) T7 DNA Polymerase (unmodified)
T5 DNA Polymerase T5 DNA Polymerase
T4-DNA Ligase T4-DNA Ligase
E. coli DNA Ligase E. coli DNA Ligase
DNA Lligase III DNA Lligase III
DNA Ligase IV DNA Ligase IV
9 Degrees North DNA Ligase 9 Degrees North DNA Ligase
Pfu DNA Ligase Pfu DNA Ligase
Taq DNA Ligase Taq DNA Ligase
T4 RNA Ligase 1 T4 RNA Ligase 1
CIP (calf intestinal phosphatase) CIP (calf intestinal phosphatase)
RNAse A RNAse A
RNAse H RNAse H
EcoRI EcoRI
M.EcoRI M.EcoRI
PstI PstI
M.PstI M.PstI
Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) (SuperScriptII) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) (SuperScriptII)
Thermus thermophilus (Tth) RT Thermus thermophilus (Tth) RT
T3 RNA Polymerase T3 RNA Polymerase
T7 RNA Polymerase T7 RNA Polymerase
SP6 RNA Polymerase SP6 RNA Polymerase


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