Open Yeast Collection for Metabolic Engineering

Open Yeast Collection for Metabolic Engineering

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Saccharomyces cerevisiae (brewers yeast) is one of the most useful microorganisms to humans with usage in brewing beer, making wine, and baking bread. In addition to these traditional uses, this yeast has been instrumental in biotechnology, being one of the primary model organisms used for industrial fermentation of bioengineered metabolic pathways. Currently, there are no free open source toolkits for engineering Saccharomyces cerevisiae, which restricts access to this powerful organism as a platform. The Open Yeast Collection is a free and open DNA toolkit for engineering Saccharomyces cerevisiae, enabling anyone to have a complete and robust toolkit to build new metabolic pathways in this yeast.


What can it be used for:

This first Open Yeast Collection is a foundational and enabling framework for contributing to the creation of an open, sustainable and equitable bioeconomy. More specifically Open Yeast Collection permits the building of plasmids from free, reusable and redistributable genetic parts for genetically modifying Saccharomyces cerevisiae. The plasmids can be used for basic education and research (e.g. protein-protein interactions via yeast-2-hybrid) or for building multi-enzyme metabolic pathways to create a wide variety of natural/commodity chemicals as well as fine and specialized chemicals such as pharmaceuticals. This will appeal to a wide variety of users from educators & students, community-based & academic researchers and bio-entrepreneurs.

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