The Open Reporter Collection includes 42 commonly used reporter genes used in all kinds of biology labs for a variety of techniques including measuring gene expression, diagnostics, verifying successful transformations and more. The collection includes chromoproteins (which produce a colour that is visible to the naked eye), fluorescent proteins (that require excitation by a certain wavelength of light and emit another wavelength), luciferases (which emit bioluminscence) and other enzymes that enable colorimetric detection of a substrate by changing its color, for example horseradish peroxidase which classically turns a chemical substrate bright pink in very common antibody-based assays such as ELISA.
What can the collection be used for?
There are so many uses for reporter genes that parts of this collection will be useful for almost any lab performing cell or molecular biology. We have provided reporters with a variety of spectra, output and maturation times to cater for specific research uses. Importantly, we cover a wide variety of colours that are visible to the naked eye and using fluorescent detection, making this collection useful not only for research but also for education and even bioart. All reporters are off-patent and can also be used commercially, which is good news for small biotech companies.
More information can be found at the Open Bioeconomy Lab Website
cjBlue/ BioBrick K592011 meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902
spisPink/spisCP Biobrick K1033925 amilCP Biobrick K592009 gfasPurple/gfasCP Biobrick K1033918 ahyaCP gdjiCP meffCP fuGFP amilCFP anobCFP2 meffCFP mmilCFP meleCFP efasCFP aacuGFP1 aacuGFP2 afraGFP amilGFP Biobrick K592010 eechGFP1 eechGFP2 eechGFP3 efasGFP gfasGFP palmGFP sarcGFP amilRFP meffRed/meffRFP Biobricks K592012 pporRFP meleRFP scubRFP eforRed/eforCP Biobrick K592012 asPink/asFP595/asCP BioBrick K1033933 amajLime/amajCFP/amFP486 BioBrick K1033916 EiraCFP Biobrick J97000 beta-galactosidase horseradish peroxidase (HRP) Firefly Luciferase [Photinus pyralis] (Fluc) Luciferase [Luciola cruciata] (Japanese firefly) Renilla luciferase beta-lactamase Calf Intestine Alkaline phosphatase (CIAP)