The Open Reporter Collection includes 32 commonly used reporter genes used in all kinds of biology labs for a variety of techniques including measuring gene expression, diagnostics, verifying successful transformations and more. The collection includes chromoproteins (which produce a colour that is visible to the naked eye), fluorescent proteins (that require excitation by a certain wavelength of light and emit another wavelength), luciferases (which emit bioluminscence) and other enzymes that enable colorimetric detection of a substrate by changing its color, for example horseradish peroxidase which classically turns a chemical substrate bright pink in very common antibody-based assays such as ELISA.
What can the collection be used for?
There are so many uses for reporter genes that parts of this collection will be useful for almost any lab performing cell or molecular biology. We have provided reporters with a variety of spectra, output and maturation times to cater for specific research uses. Importantly, we cover a wide variety of colours that are visible to the naked eye and using fluorescent detection, making this collection useful not only for research but also for education and even bioart. All reporters are off-patent and can also be used commercially, which is good news for small biotech companies.
Open Reporters are not provided in expression vectors. In order to see a color, you must subclone your gene of interest into an expression vector.
More information can be found at the Open Bioeconomy Lab Website. A plate map can be found here.
This kit is included in kit plate 2 of the 2022 iGEM distribution. The plate map for kit plate 2 of the iGEM 2022 distribution can be found here.
This product is made available under the unilateral OpenMTA.
Open Reporters is shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
Prior Kit Versions
If you received an earlier version of this kit labeled "Open Enzymes (Part 2)/Open Reporters, you can find the platemap here.
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| cjBlue/ BioBrick K592011 | cjBlue/ BioBrick K592011 | BBF10K_003331 |
| gfasPurple/gfasCP Biobrick K1033918 | gfasPurple/gfasCP Biobrick K1033918 | BBF10K_003335 |
| meffCP | meffCP | BBF10K_003338 |
| meffCFP | meffCFP | BBF10K_003342 |
| mmilCFP | mmilCFP | BBF10K_003343 |
| meleCFP | meleCFP | BBF10K_003344 |
| efasCFP | efasCFP | BBF10K_003345 |
| aacuGFP1 | aacuGFP1 | BBF10K_003346 |
| aacuGFP2 | aacuGFP2 | BBF10K_003347 |
| afraGFP | afraGFP | BBF10K_003348 |
| amilGFP Biobrick K592010 | amilGFP Biobrick K592010 | BBF10K_003349 |
| eechGFP1 | eechGFP1 | BBF10K_003350 |
| eechGFP2 | eechGFP2 | BBF10K_003351 |
| eechGFP3 | eechGFP3 | BBF10K_003352 |
| efasGFP | efasGFP | BBF10K_003353 |
| gfasGFP | gfasGFP | BBF10K_003354 |
| palmGFP | palmGFP | BBF10K_003355 |
| amilRFP | amilRFP | BBF10K_003357 |
| meffRed/meffRFP Biobricks K592012 | meffRed/meffRFP Biobricks K592012 | BBF10K_003358 |
| meleRFP | meleRFP | BBF10K_003360 |
| scubRFP | scubRFP | BBF10K_003361 |
| eforRed/eforCP Biobrick K592012 | eforRed/eforCP Biobrick K592012 | BBF10K_003362 |
| amajLime/amajCFP/amFP486 BioBrick K1033916 | amajLime/amajCFP/amFP486 BioBrick K1033916 | BBF10K_003364 |
| EiraCFP Biobrick J97000 | EiraCFP Biobrick J97000 | BBF10K_003365 |
| HRP | horseradish peroxidase (HRP) | BBF10K_003367 |
| Firefly Luciferase [Photinus pyralis] (Fluc) | Firefly Luciferase [Photinus pyralis] (Fluc) | BBF10K_003368 |
| Luciferase [Luciola cruciata] (Japanese firefly) | Luciferase [Luciola cruciata] (Japanese firefly) | BBF10K_003369 |
| Renilla luciferase | Renilla luciferase | BBF10K_003370 |
| beta-lactamase | beta-lactamase | BBF10K_003371 |
| CIAP | Calf Intestine Alkaline phosphatase (CIAP) | BBF10K_003372 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Carlos Ueira-Vieira | ueira {at} ufu {dot} br | Brazil |
| SERV0223789 CAROLINE BACQUET | caroline.bacquet {at} ikiam {dot} edu {dot} ec | United States |
| Kelly Gallagher | kelly.gallagher {at} oneonta {dot} edu | United States |
| Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
| Elena Rosca | erosca {at} ashesi {dot} edu {dot} gh | Ghana |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Emin Bursa | NA | United States |
| Lorenzo Zolfanelli | biopunk {at} zolfa {dot} nl | France |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | United States |
| Anil Challa | akchalla {at} uab {dot} edu | United States |
| Michael Powers | mpowers9304 {at} gmail {dot} com | United States |
| Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Fernan Federici | ffederici {at} bio {dot} puc {dot} cl | Chile |
| Jorge Marchand | jmarcha {at} uw {dot} edu | United States |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| John Allan | john.allan {at} eng {dot} ox {dot} ac {dot} uk | United Kingdom |
| Moritz Venne | moritz.venne {at} web {dot} de | Germany |
| Benjamin Loang | benjamin.liang {at} uq {dot} edu {dot} au | Australia |
| Mark Slabodnick | mmslabodnick {at} knox {dot} edu | United States |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Gülşen Günel | gulsen.gunel {at} ogr {dot} iu {dot} edu {dot} tr | Turkey |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Lisandro Pacheco | lpacheco28 {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| Tocvic Meng | Tocvic Meng \ Email: tocvic.meng {at} holocyte {dot} com \ website https://gmexpression {dot} com/ | Australia |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| Dr. Rajashree Patil | rajshree.patil.n {at} gmail {dot} com | India |
| John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
| Intan Taufik | i.taufik {at} sith {dot} itb {dot} ac {dot} id | Indonesia |
| Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
| Josephine Labos | N/A | Philippines |
| robson tramontina | robson.tramontina {at} gmail {dot} com | Brazil |
| Kristel Alman | kristel.alman {at} ut {dot} ee | Estonia |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Maira Goytia | mgoytia {at} spelman {dot} edu | United States |
| mehmet tardu | mtardu {at} gmail {dot} com | Turkey |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Nikolaus Dietz | nikolaus.dietz {at} unibas {dot} ch | Switzerland |
| Jose David Rosales | https://www.linkedin.com/in/david-rosales-574a7151/ | United States |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Jianxiang Shi | jianxiangshi {at} zzu {dot} edu {dot} cn | China |
| James Altamirano | James_altam {at} utexas {dot} edu | United States |
| Kevin Correia | kevin {at} kedalionlabs {dot} com | Netherlands |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Elliot Medina | elliot.medina {at} moffitt {dot} org | United States |
| Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
| Amir Kashani | NA | Canada |
| Simon Hofer | soyouwantalab {at} gmail {dot} com | Austria |
| Elizabeth Bilsland | bilsland {at} unicamp {dot} br | Brazil |
| Meng Lu | menglu {at} iastate {dot} edu | United States |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Vincent Paris | NA | United States |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| Chris Badenhorst | chris.badenhorst {at} uni-greifswald {dot} de | Germany |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Tal Globus | bio {at} talglobus {dot} com | United States |
| Sarah Ware | sarah.ware {at} bioblaze {dot} org | United States |
| Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Yi Wu | F.Nobrega {at} soton {dot} ac {dot} uk | United Kingdom |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Nadia Odaliz Chamana Chura | nadia.chamana {at} utec {dot} edu {dot} pe | France |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Emmanuele Severi | emmanuele.severi {at} newcastle {dot} ac {dot} uk | United Kingdom |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Joey Riepsaame | joey.riepsaame {at} path {dot} ox {dot} ac {dot} uk | United Kingdom |
| Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Watanyu Bunsermyos | watanyu.bu {at} ku {dot} th | Thailand |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Jehovani Lopez | jlopez {at} genspace {dot} org | United States |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Leandro Oliveira (NG) | ll637 {at} cam {dot} ac {dot} uk | United Kingdom |
| Hernán Rebolledo | lenaranjo {at} gmail {dot} com | United States |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| Jonathan LeCureux | jlecureu {at} svsuedu | United States |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| Arthur Zanetti Nunes Fernandes | fgueiros {at} iq {dot} usp {dot} br | United States |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Joao Vitor Dutra Molino | candidomolino {at} gmail {dot} com | United States |
| Shawn Lyons | smlyons1 {at} bu {dot} edu | United States |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| Vitaliy Strochkov | NA | Kazakhstan |
Download all of this information as a CSV from our GitHub.