The Open Reporter Collection includes 32 commonly used reporter genes used in all kinds of biology labs for a variety of techniques including measuring gene expression, diagnostics, verifying successful transformations and more. The collection includes chromoproteins (which produce a colour that is visible to the naked eye), fluorescent proteins (that require excitation by a certain wavelength of light and emit another wavelength), luciferases (which emit bioluminscence) and other enzymes that enable colorimetric detection of a substrate by changing its color, for example horseradish peroxidase which classically turns a chemical substrate bright pink in very common antibody-based assays such as ELISA.
What can the collection be used for?
There are so many uses for reporter genes that parts of this collection will be useful for almost any lab performing cell or molecular biology. We have provided reporters with a variety of spectra, output and maturation times to cater for specific research uses. Importantly, we cover a wide variety of colours that are visible to the naked eye and using fluorescent detection, making this collection useful not only for research but also for education and even bioart. All reporters are off-patent and can also be used commercially, which is good news for small biotech companies.
Open Reporters are not provided in expression vectors. In order to see a color, you must subclone your gene of interest into an expression vector.
More information can be found at the Open Bioeconomy Lab Website. A plate map can be found here.
This product is made available under the unilateral OpenMTA.
Open Reporters is shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
Prior Kit Versions
If you received an earlier version of this kit labeled "Open Enzymes (Part 2)/Open Reporters, you can find the platemap here.
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| cjBlue/ BioBrick K592011 | cjBlue/ BioBrick K592011 | BBF10K_003331 |
| gfasPurple/gfasCP Biobrick K1033918 | gfasPurple/gfasCP Biobrick K1033918 | BBF10K_003335 |
| meffCP | meffCP | BBF10K_003338 |
| meffCFP | meffCFP | BBF10K_003342 |
| mmilCFP | mmilCFP | BBF10K_003343 |
| meleCFP | meleCFP | BBF10K_003344 |
| efasCFP | efasCFP | BBF10K_003345 |
| aacuGFP1 | aacuGFP1 | BBF10K_003346 |
| aacuGFP2 | aacuGFP2 | BBF10K_003347 |
| afraGFP | afraGFP | BBF10K_003348 |
| amilGFP Biobrick K592010 | amilGFP Biobrick K592010 | BBF10K_003349 |
| eechGFP1 | eechGFP1 | BBF10K_003350 |
| eechGFP2 | eechGFP2 | BBF10K_003351 |
| eechGFP3 | eechGFP3 | BBF10K_003352 |
| efasGFP | efasGFP | BBF10K_003353 |
| gfasGFP | gfasGFP | BBF10K_003354 |
| palmGFP | palmGFP | BBF10K_003355 |
| amilRFP | amilRFP | BBF10K_003357 |
| meffRed/meffRFP Biobricks K592012 | meffRed/meffRFP Biobricks K592012 | BBF10K_003358 |
| meleRFP | meleRFP | BBF10K_003360 |
| scubRFP | scubRFP | BBF10K_003361 |
| eforRed/eforCP Biobrick K592012 | eforRed/eforCP Biobrick K592012 | BBF10K_003362 |
| amajLime/amajCFP/amFP486 BioBrick K1033916 | amajLime/amajCFP/amFP486 BioBrick K1033916 | BBF10K_003364 |
| EiraCFP Biobrick J97000 | EiraCFP Biobrick J97000 | BBF10K_003365 |
| HRP | horseradish peroxidase (HRP) | BBF10K_003367 |
| Firefly Luciferase [Photinus pyralis] (Fluc) | Firefly Luciferase [Photinus pyralis] (Fluc) | BBF10K_003368 |
| Luciferase [Luciola cruciata] (Japanese firefly) | Luciferase [Luciola cruciata] (Japanese firefly) | BBF10K_003369 |
| Renilla luciferase | Renilla luciferase | BBF10K_003370 |
| beta-lactamase | beta-lactamase | BBF10K_003371 |
| CIAP | Calf Intestine Alkaline phosphatase (CIAP) | BBF10K_003372 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Vitaliy Strochkov | NA | Kazakhstan |
| SERV0223789 CAROLINE BACQUET | caroline.bacquet {at} ikiam {dot} edu {dot} ec | United States |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Vincent Paris | NA | United States |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Sarah Ware | sarah.ware {at} bioblaze {dot} org | United States |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| Fernan Federici | ffederici {at} bio {dot} puc {dot} cl | Chile |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Yi Wu | F.Nobrega {at} soton {dot} ac {dot} uk | United Kingdom |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Hernán Rebolledo | lenaranjo {at} gmail {dot} com | United States |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Jose David Rosales | https://www.linkedin.com/in/david-rosales-574a7151/ | United States |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Jorge Marchand | jmarcha {at} uw {dot} edu | United States |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Jehovani Lopez | jlopez {at} genspace {dot} org | United States |
| Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
| Leandro Oliveira (NG) | ll637 {at} cam {dot} ac {dot} uk | United Kingdom |
| Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
| Emin Bursa | NA | United States |
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| James Altamirano | James_altam {at} utexas {dot} edu | United States |
| Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Kevin Correia | kevin {at} kedalionlabs {dot} com | Netherlands |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| Elliot Medina | elliot.medina {at} moffitt {dot} org | United States |
| Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
| Joey Riepsaame | joey.riepsaame {at} path {dot} ox {dot} ac {dot} uk | United Kingdom |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Dr. Rajashree Patil | rajshree.patil.n {at} gmail {dot} com | India |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Mark Slabodnick | mmslabodnick {at} knox {dot} edu | United States |
| Maira Goytia | mgoytia {at} spelman {dot} edu | United States |
| Chris Badenhorst | chris.badenhorst {at} uni-greifswald {dot} de | Germany |
| Joao Vitor Dutra Molino | candidomolino {at} gmail {dot} com | United States |
| Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
| Benjamin Loang | benjamin.liang {at} uq {dot} edu {dot} au | Australia |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Lorenzo Zolfanelli | biopunk {at} zolfa {dot} nl | France |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| Nadia Odaliz Chamana Chura | nadia.chamana {at} utec {dot} edu {dot} pe | France |
| Kristel Alman | kristel.alman {at} ut {dot} ee | Estonia |
| Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Carlos Ueira-Vieira | ueira {at} ufu {dot} br | Brazil |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
Download all of this information as a CSV from our GitHub.