Currently, sharing and reuse of plasmids is managed by material transfer agreements and patent-based property rights. Navigating these rights and agreements can place cumbersome legal burdens on researchers, who often do not have the legal training to negotiate the agreements themselves, and who may not have access to a university technology transfer office who can help them through the process.
To address these challenges, we have made our own open source plasmids of known provenance and free of IP conflict, thus removing property rights encumbrances and redistribution barriers. Here we present the Open Plasmids Collection, a growing group of standard, freely available, open-source plasmids. Plasmids in the collection can be reused, remade, and redistributed as often as one would like.
What can it be used for:
The Open Plasmids distribution is comprised of three plasmids: pBuild, pYeast, and pInducible. Each vector was designed specifically for the Open Plasmids Collection, and has both an E. coli origin of replication and an antibiotic resistance marker for negative selection.
pBuild is designed as a robust, flexible E. coli cloning vector. The plasmid backbone is compatible with multiple cloning strategies, including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard. pYeast is an expression vector designed for propagation in E. coli and galactose-inducible expression in yeast, allowing users to investigate their genes in a eukaryotic environment. pInducible is an inducible-copy vector based on iGEM plasmid pSB2K3, and intended for cloning difficult or toxic genes.
Where can I find more information:
- More information about plasmids
- Freegenes Standard MoClo sites
- Plate Layout
- pBuild genbank file
- pYeast genbank file
- pInducible genbank file
Plates in distribution: 1
Shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
Plate layout can be found here.
Instructions for Use:
Transform your plasmid of choice into competent Top10 E. coli cells (or similar). Culture at 37C overnight, with shaking at around 300rpm. The bacterial selection marker for pBuild is ampicillin, and the bacterial selection marker for pYeast and pInducible is kanamycin.
To induce pInducible’s high copy origin of replication, add IPTG to the mid-log-phase culture to a final concentration of 1mM. Allow cells to grow to saturation, then miniprep DNA.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
Genes
| Gene | Name | NCBI ID | Freegenes ID |
|---|---|---|---|
| pBuild | pOpen_v3 | N/A | BBF10K_003498 |
| pYeast | pOpen_yeast | N/A | BBF10K_003499 |
| pInducible | pOpen_v4 | N/A | BBF10K_000589 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Ed Eisenstein | eisenste {at} umd {dot} edu | United States |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Maira Goytia | mgoytia {at} spelman {dot} edu | United States |
| Alexandr Dorif | dorif11 {at} gmail {dot} com | Moldova, Republic of |
| Kheng Oon Low | khengoon {at} nibm {dot} my | Malaysia |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Maan Neamah | maanm.neamah {at} uokufa {dot} edu {dot} iq | Iraq |
| Keoni Gandall | keoni {at} sporenetlabs {dot} com | United States |
| Kevin Correia | kevin {at} kedalionlabs {dot} com | Netherlands |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Kristel Alman | kristel.alman {at} ut {dot} ee | Estonia |
| Lorenzo Zolfanelli | biopunk {at} zolfa {dot} nl | France |
| Josephine Labos | N/A | Philippines |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Dr. Rajashree Patil | rajshree.patil.n {at} gmail {dot} com | India |
| Wilson Teran | wteran.doc {at} gmail {dot} com | Colombia |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Johan Sosa | johan {at} thefunquantum {dot} com | United States |
| Andrei Berasneu | a.berasneu {at} kleverlab {dot} pl | Poland |
| Tania Pozzo | tania.pozzo {at} gmail {dot} com | Sweden |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Keoni Gandall | keonigandall.com | United States |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Norhan Hassan | norhan.hassan {at} kaust {dot} edu {dot} sa | Saudi Arabia |
| Travis Wertz | travis {at} traviswertz {dot} com | United States |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| Hernán Rebolledo | lenaranjo {at} gmail {dot} com | United States |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| raghav sridhar | raghav {at} cambrianbioworks {dot} com | India |
| Joao Vitor Dutra Molino | candidomolino {at} gmail {dot} com | United States |
| Amjed Alsultan | NA | Iraq |
| Ian Cubit | Djcubit {at} nycap {dot} are {dot} com | United States |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Rolando Perez | rcperez {at} alumni {dot} stanford {dot} edu | United States |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Dayananda Chandrappa | nanda.daya {at} gmail {dot} com | India |
| Julio Bonilla | jabonill {at} espol {dot} edu {dot} ec | Ecuador |
| Nikolaus Dietz | nikolaus.dietz {at} unibas {dot} ch | Switzerland |
| Danielle Pedrolli | danielle.pedrolli {at} unesp {dot} br | Brazil |
| Vincent Paris | NA | United States |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Tocvic Meng | Tocvic Meng \ Email: tocvic.meng {at} holocyte {dot} com \ website https://gmexpression {dot} com/ | Australia |
| Miles Rogers | miles.rogers {at} raytheon {dot} com | United States |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
| Rodrigo Caroca | rcaroca {at} uazuay {dot} edu {dot} ec | Ecuador |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Scott Pownall | scott {at} opensciencenet {dot} org | Canada |
| James Altamirano | James_altam {at} utexas {dot} edu | United States |
| Anil Challa | akchalla {at} uab {dot} edu | United States |
| Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| mehmet tardu | mtardu {at} gmail {dot} com | Turkey |
| Dong Nguyen Tam | NA | Vietnam |
| Jae-Seong Yang | jaeseong.yang {at} cragenomica {dot} es | Spain |
| Tal Globus | bio {at} talglobus {dot} com | United States |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Nadia Odaliz Chamana Chura | nadia.chamana {at} utec {dot} edu {dot} pe | France |
| Emin Bursa | NA | United States |
| Elizabeth Bilsland | bilsland {at} unicamp {dot} br | Brazil |
| Jonathan Jonathan | Jonathancontact16 {at} gmail {dot} com | Indonesia |
| Sewa Singh | sewasingh {at} rnavaxbio {dot} com | India |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| Ben Davis | bed58 {at} aber {dot} ac {dot} uk | United Kingdom |
| Stephen Klusza | StephenKlusza {at} clayton {dot} edu | United States |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Gülşen Günel | gulsen.gunel {at} ogr {dot} iu {dot} edu {dot} tr | Turkey |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| Matias Cabeza | matiascabeza {at} gmail {dot} com | Argentina |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| Simon Hofer | soyouwantalab {at} gmail {dot} com | Austria |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| semih cagan | semihcgn {at} gmail {dot} com | Turkey |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Vitaliy Strochkov | NA | Kazakhstan |
Download all of this information as a CSV from our GitHub.