Currently, sharing and reuse of plasmids is managed by material transfer agreements and patent-based property rights. Navigating these rights and agreements can place cumbersome legal burdens on researchers, who often do not have the legal training to negotiate the agreements themselves, and who may not have access to a university technology transfer office who can help them through the process.
To address these challenges, we have made our own open source plasmids of known provenance and free of IP conflict, thus removing property rights encumbrances and redistribution barriers. Here we present the Open Plasmids Collection, a growing group of standard, freely available, open-source plasmids. Plasmids in the collection can be reused, remade, and redistributed as often as one would like.
What can it be used for:
The Open Plasmids distribution is comprised of three plasmids: pBuild, pYeast, and pInducible. Each vector was designed specifically for the Open Plasmids Collection, and has both an E. coli origin of replication and an antibiotic resistance marker for negative selection.
pBuild is designed as a robust, flexible E. coli cloning vector. The plasmid backbone is compatible with multiple cloning strategies, including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard. pYeast is an expression vector designed for propagation in E. coli and galactose-inducible expression in yeast, allowing users to investigate their genes in a eukaryotic environment. pInducible is an inducible-copy vector based on iGEM plasmid pSB2K3, and intended for cloning difficult or toxic genes.
Where can I find more information:
- More information about plasmids
- Freegenes Standard MoClo sites
- Plate Layout
- pBuild genbank file
- pYeast genbank file
- pInducible genbank file
Plates in distribution: 1
All parts are present on the plate as raw DNA.
Instructions for Use:
Transform your plasmid of choice into competent Top10 E. coli cells (or similar). Culture at 37C overnight, with shaking at around 300rpm. The bacterial selection marker for pBuild is ampicillin, and the bacterial selection marker for pYeast and pInducible is kanamycin.
To induce pInducible’s high copy origin of replication, add IPTG to the mid-log-phase culture to a final concentration of 1mM. Allow cells to grow to saturation, then miniprep DNA.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
Genes
| Gene | Name | NCBI ID | Freegenes ID |
|---|---|---|---|
| pBuild | pOpen_v3 | N/A | BBF10K_003498 |
| pYeast | pOpen_yeast | N/A | BBF10K_003499 |
| pInducible | pOpen_v4 | N/A | BBF10K_000589 |
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Emin Bursa | NA | United States |
| Ed Eisenstein | eisenste {at} umd {dot} edu | United States |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Keoni Gandall | keoni {at} sporenetlabs {dot} com | United States |
| Dong Nguyen Tam | NA | Vietnam |
| raghav sridhar | raghav {at} cambrianbioworks {dot} com | India |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Jae-Seong Yang | jaeseong.yang {at} cragenomica {dot} es | Spain |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Ian Cubit | Djcubit {at} nycap {dot} are {dot} com | United States |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| Tania Pozzo | tania.pozzo {at} gmail {dot} com | Sweden |
| semih cagan | semihcgn {at} gmail {dot} com | Turkey |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Rodrigo Caroca | rcaroca {at} uazuay {dot} edu {dot} ec | Ecuador |
| Julio Bonilla | jabonill {at} espol {dot} edu {dot} ec | Ecuador |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Travis Wertz | travis {at} traviswertz {dot} com | United States |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Johan Sosa | johan {at} thefunquantum {dot} com | United States |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| James Altamirano | James_altam {at} utexas {dot} edu | United States |
| Vitaliy Strochkov | NA | Kazakhstan |