One-Step Cloning and Chromosomal Integration of DNA

One-Step Cloning and Chromosomal Integration of DNA

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What is it?

“Clonetegration” is a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.

 

What can it be used for?

Integrating your favorite DNA into specific loci on a bacterial chromosome.

 

Where can I find more information?

One-Step Cloning and Chromosomal Integration of DNA, François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin, https://pubs.acs.org/doi/full/10.1021/sb400021j?src=recsys&

 

All genes are present on the plate in E. coli glycerol stocks. If you've already received the plate, check out the plate layout. Plates are labelled "One Step Integration Collection"

 

Genes

Gene Name Freegenes ID
pE-FLP pE-FLP BBF10K_000016
pOSIP-CH (CamR, HK022) pOSIP-CH (CamR, HK022) BBF10K_000031
pOSIP-KO (KanR, 186) pOSIP-KO (KanR, 186) BBF10K_000492
pOSIP-TT (TetR, P21) pOSIP-TT (TetR, P21) BBF10K_000493

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact Country
Julie Legault julie {at} amino {dot} bio Canada
Ahmed Hegazy a.hegazy {at} biotec {dot} rwth-aachen {dot} de Germany
Jae-Seong Yang jaeseong.yang {at} cragenomica {dot} es Spain
Fernan Federici ffederici {at} bio {dot} puc {dot} cl Chile
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org United States
Han Teng Wong wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg Singapore
Franklin Nobrega f.nobrega {at} soton {dot} ac {dot} uk United Kingdom
robson tramontina robson.tramontina {at} gmail {dot} com Brazil
Ahmed Atef ahmed_atefaig2 {at} yahoo {dot} com Saudi Arabia
Kenneth Frimpong ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh Ghana
Keoni Gandall koeng101 {at} gmail {dot} com United States
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