One-Step Cloning and Chromosomal Integration of DNA

One-Step Cloning and Chromosomal Integration of DNA

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What is it?

“Clonetegration” is a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.

 

What can it be used for?

Integrating your favorite DNA into specific loci on a bacterial chromosome.

 

Where can I find more information?

One-Step Cloning and Chromosomal Integration of DNA, François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin, https://pubs.acs.org/doi/full/10.1021/sb400021j?src=recsys&

 

All genes are present on the plate in E. coli glycerol stocks. If you've already received the plate, check out the plate layout.

 

Genes

Gene Name
pE-FLP pE-FLP
pOSIP-CH (CamR, HK022) pOSIP-CH (CamR, HK022)
pOSIP-KO (KanR, 186) pOSIP-KO (KanR, 186)
pOSIP-TT (TetR, P21) pOSIP-TT (TetR, P21)

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact
Jae-Seong Yang jaeseong.yang {at} cragenomica {dot} es
Franklin Nobrega f.nobrega {at} soton {dot} ac {dot} uk
Fernan Federici ffederici {at} bio {dot} puc {dot} cl
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org
Julie Legault julie {at} amino {dot} bio
Keoni Gandall koeng101 {at} gmail {dot} com
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