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Mycoplasma pneumoniae genes (E. coli codon optimized)

Mycoplasma pneumoniae genes (E. coli codon optimized)

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Mycoplasma pneumoniae is a close relative to Mycoplasma mycoides and Mycoplasma genitalium, and is known to cause disease in humans. Being able to rapidly test differences between those 3 genomes is necessary for synthetic genome research, and the genes themselves are important for biomedical research to find new drug targets. However, there is no resource for Mycoplasma pneumoniae genes to conduct this research. To enable comparative synthetic genome research and biomedical advancements, FreeGenes has synthesized 189 of 690 Mycoplasma pneumoniae M129 genes, codon optimized for Escherichia coli. Hopefully, by supporting Mycoplasma research, FreeGenes can encourage synthetic biology laboratories to conduct synthetic genome research.

What is it
This is a collection of as many protein coding sequences from Mycoplasma pneumoniae M129 as FreeGenes could clone in a single round of cloning. They are all standardized for MoClo assembly and codon optimized for Escherichia coli.

What can it be used for
There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Mycoplasma genome.

Shipped as purified and dried down plasmid DNA stained with Cresol Red in two 96-well plates. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation. This product is made available under the unilateral OpenMTA

Where I can find more information

189 unique parts

Instructions for Use:

Transform into Top10 E. coli or equivalent. Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.

PlateSet: Mpneumonia-0 Mpneumonia-1

Plate Layout



Gene Name NCBI ID Freegenes ID
xdj1 DnaJ domain-containing protein NP_109690.1 BBF10K_002428
yabD deoxyribonuclease NP_109697.1 BBF10K_002435
MPN013 hypothetical protein NP_109701.1 BBF10K_002439
rpoE DNA-directed RNA polymerase subunit delta NP_109712.1 BBF10K_002450
MPN032 hypothetical protein NP_109720.1 BBF10K_002458
upp uracil phosphoribosyltransferase NP_109721.1 BBF10K_002459
MPN038 hypothetical protein NP_109726.1 BBF10K_002464
glpF glycerol uptake facilitator NP_109731.1 BBF10K_002466
MPN047 nicotinate phosphoribosyltransferase NP_109735.1 BBF10K_002471
glpK glycerol kinase NP_109738.1 BBF10K_002476
MPN054 hypothetical protein NP_109742.1 BBF10K_002480
potB spermidine/putrescine transport system permease NP_109744.1 BBF10K_002482
gcp tRNA N6-adenosine threonylcarbamoyltransferase NP_109747.1 BBF10K_002485
metX S-adenosylmethionine synthase NP_109748.1 BBF10K_002486
deoD purine nucleoside phosphorylase NP_109750.1 BBF10K_002489
deoA thymidine phosphorylase NP_109752.1 BBF10K_002491
yabF ribonuclease M5 NP_109760.1 BBF10K_002499
fruK 1-phosphofructokinase NP_109767.1 BBF10K_002506
MPN083 lipoprotein NP_109771.1 BBF10K_002510
MPN085 hypothetical protein NP_109773.1 BBF10K_002512
MPN087 hypothetical protein NP_109775.1 BBF10K_002514
hsdS hypothetical protein NP_109777.1 BBF10K_002516
MPN094 hypothetical protein NP_109782.1 BBF10K_002518
MPN095 hypothetical protein NP_109783.1 BBF10K_002519
MPN096 hypothetical protein NP_109784.1 BBF10K_002520
MPN101 hypothetical protein NP_109789.1 BBF10K_002522
MPN103 hypothetical protein NP_109791.1 BBF10K_002523
MPN104 hypothetical protein NP_109792.1 BBF10K_002524
pheS phenylalanine--tRNA ligase subunit alpha NP_109793.1 BBF10K_002526
MPN110 hypothetical protein NP_109798.1 BBF10K_002531
MPN112 hypothetical protein NP_109800.1 BBF10K_002533
infC translation initiation factor IF-3 NP_109803.1 BBF10K_002535
MPN126 metallophosphoesterase NP_109814.1 BBF10K_002546
MPN129 hypothetical protein NP_109817.1 BBF10K_002547
MPN130 hypothetical protein NP_109818.1 BBF10K_002548
ugpC ABC transporter ATP-binding protein NP_109822.1 BBF10K_002552
ugpE ABC transporter permease NP_109824.1 BBF10K_002555
MPN138 hypothetical protein NP_109826.1 BBF10K_002557
MPN145 hypothetical protein NP_109833.1 BBF10K_002564
rbfA ribosome-binding factor A NP_109844.1 BBF10K_002575
yaaC bifunctional riboflavin kinase/FMNadenylyltransferase NP_109846.1 BBF10K_002577
MPN160 hypothetical protein NP_109848.1 BBF10K_002579
MPN162 hypothetical protein NP_109850.1 BBF10K_002581
MPN163 hypothetical protein NP_109851.1 BBF10K_002582
rplW 50S ribosomal protein L23 NP_109855.1 BBF10K_002586
rpsC 30S ribosomal protein S3 NP_109859.1 BBF10K_002590
rplP 50S ribosomal protein L16 NP_109860.1 BBF10K_002591
rpmC 50S ribosomal protein L29 NP_109861.1 BBF10K_002592
rplN 50S ribosomal protein L14 NP_109863.1 BBF10K_002594
rplO 50S ribosomal protein L15 NP_109871.1 BBF10K_002603
secY preprotein translocase subunit SecY NP_109872.1 BBF10K_002604
adk adenylate kinase NP_109873.1 BBF10K_002605
rpoA DNA-directed RNA polymerase subunit alpha NP_109879.1 BBF10K_002611
rpsB 30S ribosomal protein S2 NP_109896.1 BBF10K_002630
MPN212 hypothetical protein NP_109900.1 BBF10K_002634
MPN214 hypothetical protein NP_109902.1 BBF10K_002636
oppB oligopeptide ABC transporter permease NP_109903.1 BBF10K_002637
oppD oligopeptide ABC transporter ATP-bindingprotein NP_109905.1 BBF10K_002639
rplK 50S ribosomal protein L11 NP_109907.1 BBF10K_002642
rplA 50S ribosomal protein L1 NP_109908.1 BBF10K_002643
lgt prolipoprotein diacylglyceryl transferase NP_109912.1 BBF10K_002647
ssb single-stranded DNA binding protein NP_109917.1 BBF10K_002653
rplI 50S ribosomal protein L9 NP_109919.1 BBF10K_002655
gatB aspartyl/glutamyl-tRNA amidotransferase subunitB NP_109926.1 BBF10K_002662
MPN239 hypothetical protein NP_109927.1 BBF10K_002663
trxB thioredoxin reductase NP_109928.1 BBF10K_002664
gmk guanylate kinase NP_109934.1 BBF10K_002670
yjeQ ribosome biogenesis GTPase RsgA NP_109937.1 BBF10K_002673
galE UDP-glucose 4-epimerase NP_109945.1 BBF10K_002683
MPN259 ABC transporter permease NP_109947.1 BBF10K_002685
rbsC ABC transporter permease NP_109948.1 BBF10K_002686
MPN264 hypothetical protein NP_109952.1 BBF10K_002690
trpS tryptophan--tRNA ligase NP_109953.1 BBF10K_002691
MPN268 PTS system transporter subunit IIBC NP_109956.1 BBF10K_002694
MPN271 lipoprotein NP_109959.1 BBF10K_002698
hit1 hydrolase NP_109961.1 BBF10K_002701
MPN276 hypothetical protein NP_109964.1 BBF10K_002704
prrB type I restriction-modification systemspecificity protein NP_109973.2 BBF10K_002712
MPN290 hypothetical protein NP_109978.1 BBF10K_002718
yceC RNA pseudouridine synthase NP_109980.1 BBF10K_002720
MPN295 hypothetical protein NP_109983.1 BBF10K_002723
acpS holo-ACP synthase NP_109986.1 BBF10K_002726
pfkA ATP-dependent 6-phosphofructokinase NP_109990.1 BBF10K_002730
arcC carbamate kinase NP_109995.1 BBF10K_002733
mraW rRNA small subunit methyltransferase H NP_110003.1 BBF10K_002741
nrdF ribonucleotide-diphosphate reductase subunitbeta NP_110010.1 BBF10K_002749
nrdI ribonucleotide reductase stimulatory protein NP_110011.1 BBF10K_002750
rplU 50S ribosomal protein L21 NP_110013.1 BBF10K_002752
rpmA 50S ribosomal protein L27 NP_110015.1 BBF10K_002754
MPN329 ferric uptake regulator NP_110017.1 BBF10K_002756
tig trigger factor NP_110019.1 BBF10K_002758
bcrA ABC transporter ATP-binding protein NP_110022.1 BBF10K_002761
MPN337 hypothetical protein NP_110025.1 BBF10K_002764
MPN338 hypothetical protein NP_110026.1 BBF10K_002765
MPN344 hypothetical protein NP_110032.1 BBF10K_002771
MPN348 5-formyltetrahydrofolate cyclo-ligase NP_110036.1 BBF10K_002774
ygiH glycerol-3-phosphate acyltransferase NP_110038.1 BBF10K_002776
MPN351 Putative tRNA methyltransferase MPN_351 NP_110039.1 BBF10K_002777
MPN359 hypothetical protein NP_110047.1 BBF10K_002786
fpg formamidopyrimidine-DNA glycosylase NP_110068.1 BBF10K_002805
MPN387 hypothetical protein NP_110075.1 BBF10K_002812
lplA lipoate--protein ligase NP_110077.1 BBF10K_002813
pdhD pyruvate dehydrogenase complex dihydrolipoamidedehydrogenase NP_110078.1 BBF10K_002814
pdhB pyruvate dehydrogenase complex component E1subunit beta NP_110080.1 BBF10K_002817
greA transcription elongation factor GreA NP_110089.1 BBF10K_002827
proS proline--tRNA ligase NP_110090.1 BBF10K_002828
MPN403 hypothetical protein NP_110091.1 BBF10K_002829
MPN408 lipoprotein NP_110096.1 BBF10K_002836
MPN410 hypothetical protein NP_110098.1 BBF10K_002838
MPN414 hypothetical protein NP_110102.1 BBF10K_002843
P29 ABC transporter ATP-binding protein NP_110104.1 BBF10K_002845
MPN419 Holliday junction resolvase NP_110106.1 BBF10K_002847
glpQ glycerophosphoryl diester phosphodiesterase NP_110108.1 BBF10K_002849
yidA phosphatase NP_110115.1 BBF10K_002856
cbiO cobalt ABC transporter ATP-binding protein NP_110121.1 BBF10K_002862
come3 hypothetical protein NP_110139.1 BBF10K_002877
MPN462 hypothetical protein NP_110150.1 BBF10K_002885
MPN469 hypothetical protein NP_110157.1 BBF10K_002890
degV hypothetical protein NP_110160.1 BBF10K_002893
MPN478 transcriptional regulator NP_110166.1 BBF10K_002900
MPN479 FMN-dependent NADH-azoreductase NP_110167.1 BBF10K_002901
yibD diacylglycerol beta-glycosyltransferase NP_110171.1 BBF10K_002906
MPN484 hypothetical protein NP_110172.1 BBF10K_002907
MPN488 hypothetical protein NP_110176.1 BBF10K_002910
recA recombinase A NP_110178.1 BBF10K_002914
MPN491 hypothetical protein NP_110179.1 BBF10K_002915
yjfU PTS system ascorbate-specific transportersubunit IIA NP_110182.1 BBF10K_002919
araD L-ribulose-5-phosphate 4-epimerase NP_110186.1 BBF10K_002923
MPN501 hypothetical protein NP_110189.1 BBF10K_002926
MPN504 hypothetical protein NP_110192.1 BBF10K_002928
MPN512 hypothetical protein NP_110200.1 BBF10K_002937
MPN513 hypothetical protein NP_110201.1 BBF10K_002938
MPN514 hypothetical protein NP_110202.1 BBF10K_002939
MPN517 hypothetical protein NP_110205.1 BBF10K_002942
ygl3 Putative tRNA (cytidine(34)-2'-O)-methyltransferase NP_110209.1 BBF10K_002946
MPN524 hypothetical protein NP_110212.1 BBF10K_002949
MPN527 hypothetical protein NP_110215.1 BBF10K_002952
ppa inorganic pyrophosphatase NP_110216.1 BBF10K_002953
MPN530 hypothetical protein NP_110219.1 BBF10K_002956
ackA acetate kinase NP_110222.1 BBF10K_002959
MPN534 hypothetical protein NP_110223.1 BBF10K_002960
fmt methionyl-tRNA formyltransferase NP_110232.1 BBF10K_002971
plsX phosphate acyltransferase NP_110235.1 BBF10K_002974
thrSv threonine--tRNA ligase NP_110242.1 BBF10K_002981
MPN554 singe-stranded DNA-binding protein NP_110243.1 BBF10K_002982
argS arginine--tRNA ligase NP_110245.1 BBF10K_002984
gidB rRNA small subunit methyltransferase G NP_110247.1 BBF10K_002986
MPN559 hypothetical protein NP_110248.1 BBF10K_002987
udk uridine kinase NP_110250.1 BBF10K_002989
outB NH(3)-dependent NAD(+) synthetase NP_110251.1 BBF10K_002990
MPN565 hypothetical protein NP_110254.2 BBF10K_002993
glpQ glycerophosphoryl diester phosphodiesterase NP_110255.1 BBF10K_002994
MPN572 cytosol aminopeptidase NP_110261.1 BBF10K_003000
MPN578 hypothetical protein NP_110267.1 BBF10K_003006
MPN579 hypothetical protein NP_110268.1 BBF10K_003007
MPN580 hypothetical protein NP_110269.1 BBF10K_003008
MPN581 hypothetical protein NP_110270.1 BBF10K_003009
MPN584 hypothetical protein NP_110273.1 BBF10K_003013
MPN586 hypothetical protein NP_110275.1 BBF10K_003015
MPN587 lipoprotein NP_110276.1 BBF10K_003016
MPN589 hypothetical protein NP_110278.1 BBF10K_003018
MPN591 hypothetical protein NP_110280.1 BBF10K_003020
MPN593 hypothetical protein NP_110282.1 BBF10K_003023
atpC ATP synthase subunit epsilon NP_110286.1 BBF10K_003027
atpD ATP synthase subunit beta NP_110287.1 BBF10K_003028
atpH ATP synthase subunit delta NP_110290.1 BBF10K_003031
MPN605 hypothetical protein NP_110294.1 BBF10K_003035
eno enolase NP_110295.1 BBF10K_003036
pstB phosphate ABC transporter ATP-binding protein NP_110298.1 BBF10K_003039
MPN613 hypothetical protein NP_110302.1 BBF10K_003043
MPN614 hypothetical protein NP_110303.1 BBF10K_003044
rpsI 30S ribosomal protein S9 NP_110305.1 BBF10K_003046
rplM 50S ribosomal protein L13 NP_110306.1 BBF10K_003047
MPN626 hypothetical protein NP_110315.1 BBF10K_003056
ptsI phosphoenolpyruvate-protein phosphotransferase NP_110316.1 BBF10K_003057
tpiA triosephosphate isomerase NP_110318.1 BBF10K_003059
MPN638 type I restriction-modification systemspecificity protein NP_110327.1 BBF10K_003066
MPN641 lipoprotein NP_110330.1 BBF10K_003069
MPN642 lipoprotein NP_110331.1 BBF10K_003070
MPN644 lipoprotein NP_110333.1 BBF10K_003072
MPN648 hypothetical protein NP_110337.1 BBF10K_003076
mtlA PTS system mannitol-specific transporter subunitIIBC NP_110340.1 BBF10K_003079
MPN657 hypothetical protein NP_110346.1 BBF10K_003086
trmD NP_110348.1 BBF10K_003088
pilB methionine sulfoxide reductase B NP_110351.1 BBF10K_003091
degV hypothetical protein NP_110353.1 BBF10K_003093
MPN666 hypothetical protein NP_110355.1 BBF10K_003095
MPN673 hypothetical protein NP_110362.1 BBF10K_003103
cysA ABC transporter ATP-binding protein NP_110374.1 BBF10K_003114

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The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact Country
Amjed Alsultan amjed.talib {at} qu {dot} edu {dot} iq Iraq
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org United States
Ery Fukushima ery.fukushima {at} ikiam {dot} edu {dot} ec Ecuador
Rodolfo Marcolino rodolfo.marcolino {at} me {dot} com Argentina
Majed Alghamdi mafa2 {at} le {dot} ac {dot} uk United Kingdom

Download all of this information as a CSV from our GitHub.