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A 3 enzyme pathway coding for fungal bioluminescence, codon optimized for Nicotiana benthamiana in MoClo-compatible high-copy ampicillin resistance backbones.
One of the most requested and inspiring biotech projects has been to make glowing plants. Recently, a group out of Moscow has successfully
created a glowing plant that is visible with the naked eye. They were able to do this by using the fungal bioluminescence pathway instead of the bacterial bioluminescence pathway, which increased the efficiency of light production in these eukaryotic organisms.
Documentation
This distribution comprises of {13} genetic parts. {7} of these parts are Luz and Luz alternatives, the main light producing enzyme. {3} of these parts are 2 enzymes that complete the biosynthetic pathway necessary to sustain light production. 1 enzyme, HispS, has been split into 2 parts because it was too large for synthesis directly. It can be put back together with BglII or BsaI with the overhang sequence of GATC. The remaining {3} parts are vector components to put test these genes quickly while a FreeGenes Plant toolkit is put together.
Shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation. The plate map can be found here.
Full history of modifications are available in the snapgene files in sequence_files.zip. Genbank files are also provided in genbank_files.zip. The exact sequences ordered from Twist are available in glowing_plant_order.csv. The genes are flanked by experimentally validated orthogonal primer pairs, and a I-SceI barcode. Full set of primer pairs for all FreeGenes parts are in a git repo. Detailed information on changes can be found in the curators_notes.md.
Note: Partial plasmid duplication has been found for BBF10K_003492 (H3H test vector). This partial duplication should not impact transformation, outgrowth or primer attachment.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
Genes
Gene |
Name |
Freegenes ID |
Luz |
Fungal Luciferase |
BBF10K_003480 |
Luz-Homologue |
Fungal Luciferase-Homologue |
BBF10K_003481 |
Luz-Mc |
Mycena citricolor Fungal Luciferase |
BBF10K_003482 |
Luz-Oo |
Omphalotus olearius Fungal Luciferase |
BBF10K_003483 |
Luz-RFP |
Fungal Luciferase RFP fusion |
BBF10K_003484 |
Luz-Y189F |
Fungal Luciferase Y189F pocket mutation |
BBF10K_003485 |
Luz-Y233F |
Fungal Luciferase Y233F pocket mutation |
BBF10K_003486 |
H3H |
hispidin-3-hydroxylase |
BBF10K_003487 |
HispS_1 |
hispidin synthase |
BBF10K_003488 |
HispS_2 |
hispidin synthase |
BBF10K_003489 |
Vector-Luz |
Luz test vector |
BBF10K_003490 |
Vector-HispS |
HispS test vector |
BBF10K_003491 |
Vector-H3H |
H3H test vector |
BBF10K_003492 |
Download all of this information as
a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.
Name |
Contact |
Country |
Jordan Gonzalez |
jgonzalez {at} thecitizensciencelab {dot} org |
United States |
Travis Wertz |
travis {at} traviswertz {dot} com |
United States |
Erman ÖZER |
aracnoid {at} gmail {dot} com |
Turkey |
Jehovani Lopez |
NA |
United States |
Wei-Min Chang |
WeiMinChang {at} tmu {dot} edu {dot} tw |
Taiwan |
Amjed Alsultan |
amjed.talib {at} qu {dot} edu {dot} iq |
Iraq |
Peer Schenk |
reuben.brown {at} uqconnect {dot} edu {dot} au |
Australia |
Ben Daniels |
bndaniel {at} calpoly {dot} edu |
United States |
Dong Nguyen Tam |
NA |
Vietnam |
Cihan Aydin |
cihan.aydin {at} medeniyet {dot} edu {dot} tr |
Turkey |
Ery Fukushima |
ery.fukushima {at} ikiam {dot} edu {dot} ec |
Ecuador |
Kitty Martens |
kitmartens {at} gmail {dot} com |
Canada |
Hernán Rebolledo |
lenaranjo {at} gmail {dot} com |
United States |
James Altamirano |
James_altam {at} yahoo {dot} com |
United States |
Ahmed Hegazy |
a.hegazy {at} biotec {dot} rwth-aachen {dot} de |
Germany |
Mikhail Khvochtchev |
mikhail.khv {at} mahidol {dot} edu |
Thailand |
Ahmed Atef |
ahmed_atefaig2 {at} yahoo {dot} com |
United States |
Eric Theis |
Aracaifon {at} gmail {dot} com |
United States |
Majed Alghamdi |
mafa2 {at} le {dot} ac {dot} uk |
United Kingdom |
Foong-Jing Goh |
wind1989 {at} hotmail {dot} com |
Taiwan |
Sreenivas Eadara |
sreeni.eadara {at} gmail {dot} com |
United States |
Tomasz Jurkowski |
jurkowskit {at} cardiff {dot} ac {dot} uk |
United Kingdom |
Download all of this information as
a CSV from our GitHub.
MTA
Unilateral OpenMTA
NOTICE OF CONDITIONS
Upon acceptance and use of this Material, the Recipient agrees to the following conditions:
- Use: The Recipient shall use, store, and dispose of the Material and any Modifications in accordance with good laboratory practice and shall ensure compliance with all applicable laws, rules, and regulations, including laws, rules, and regulations governing export control and safety.
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DEFINITIONS:
- Material: Original Material, Progeny, and Unmodified Derivatives. The Material shall not include: (a) Modifications, or (b) other substances created by the Recipient through the use of the Material, which are not Modifications, Progeny, or Unmodified Derivatives.
- Progeny: Unmodified descendant from the Material, such as virus from virus, cell from cell, or organism from organism.
- Unmodified Derivatives: Substances created by the Recipient which constitute an unmodified functional subunit or product expressed by the Original Material. Some examples include: subclones of unmodified cell lines, purified or fractionated subsets of the Original Material, proteins expressed by DNA/RNA supplied by Provider, or monoclonal antibodies secreted by a hybridoma cell line.
- Modifications: Substances created by the Recipient which contain/incorporate the Material.
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