
Documentation
This distribution comprises of {13} genetic parts. {7} of these parts are Luz and Luz alternatives, the main light producing enzyme. {3} of these parts are 2 enzymes that complete the biosynthetic pathway necessary to sustain light production. 1 enzyme, HispS, has been split into 2 parts because it was too large for synthesis directly. It can be put back together with BglII or BsaI with the overhang sequence of GATC. The remaining {3} parts are vector components to put test these genes quickly while a FreeGenes Plant toolkit is put together.
Shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation. The plate map can be found here.
Full history of modifications are available in the snapgene files in sequence_files.zip. Genbank files are also provided in genbank_files.zip. The exact sequences ordered from Twist are available in glowing_plant_order.csv. The genes are flanked by experimentally validated orthogonal primer pairs, and a I-SceI barcode. Full set of primer pairs for all FreeGenes parts are in a git repo. Detailed information on changes can be found in the curators_notes.md.
Note: Partial plasmid duplication has been found for BBF10K_003492 (H3H test vector). This partial duplication should not impact transformation, outgrowth or primer attachment.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
Genes
Gene | Name | Freegenes ID |
---|---|---|
Luz | Fungal Luciferase | BBF10K_003480 |
Luz-Homologue | Fungal Luciferase-Homologue | BBF10K_003481 |
Luz-Mc | Mycena citricolor Fungal Luciferase | BBF10K_003482 |
Luz-Oo | Omphalotus olearius Fungal Luciferase | BBF10K_003483 |
Luz-RFP | Fungal Luciferase RFP fusion | BBF10K_003484 |
Luz-Y189F | Fungal Luciferase Y189F pocket mutation | BBF10K_003485 |
Luz-Y233F | Fungal Luciferase Y233F pocket mutation | BBF10K_003486 |
H3H | hispidin-3-hydroxylase | BBF10K_003487 |
HispS_1 | hispidin synthase | BBF10K_003488 |
HispS_2 | hispidin synthase | BBF10K_003489 |
Vector-Luz | Luz test vector | BBF10K_003490 |
Vector-HispS | HispS test vector | BBF10K_003491 |
Vector-H3H | H3H test vector | BBF10K_003492 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.Name | Contact | Country |
---|---|---|
Libby Sharpe | libby.sharpe {at} leomail {dot} tamuc {dot} edu | United States |
Wapouo Fadanka Stephane | mboalab {at} gmail {dot} com | Republic of Cameroon |
Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
YANG ZHANG | Yang.Zhang {at} vub {dot} be | Belgium |
Eric Theis | Aracaifon {at} gmail {dot} com | United States |
Hernán Rebolledo | lenaranjo {at} gmail {dot} com | United States |
Ben Daniels | bndaniel {at} calpoly {dot} edu | United States |
Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
Moritz Venne | moritz.venne {at} web {dot} de | Germany |
Tal Globus | bio {at} talglobus {dot} com | United States |
Jehovani Lopez | NA | United States |
Thooyavan George | www.hetrogenebiotech.com | India |
Travis Wertz | travis {at} traviswertz {dot} com | United States |
Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
Jonathan LeCureux | jlecureu {at} svsuedu | United States |
Ery Fukushima | ery.fukushima {at} ikiam {dot} edu {dot} ec | Ecuador |
Kitty Martens | kitmartens {at} gmail {dot} com | Canada |
Ismael Gonzalez | linjo200 {at} gmail {dot} com | United States |
Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | United States |
Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
Jimmy Gledson Hayden Linhares | haydenlinhars {at} gmail {dot} com | Brazil |
Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
Dong Nguyen Tam | NA | Vietnam |
Josephine Labos | N/A | Philippines |
Piero Beraun | piero.beraun {at} utec {dot} edu {dot} pe | Italy |
Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
James Altamirano | James_altam {at} yahoo {dot} com | United States |
Amjed Alsultan | amjed.talib {at} qu {dot} edu {dot} iq | Iraq |
Yuwei Jia | NA | Belgium |
Mikhail Khvochtchev | mikhail.khv {at} mahidol {dot} edu | Thailand |
Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
Joey Van Koningsbruggen | joey {at} jimmyjoy {dot} com | Netherlands |
Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
Download all of this information as a CSV from our GitHub.