Glowing Plant

Regular price: $0.00
Sale price: $0.00
Regular price
Unit price: per

Free Shipping

A 3 enzyme pathway coding for fungal bioluminescence, codon optimized for Nicotiana benthamiana in MoClo-compatible high-copy ampicillin resistance backbones.

One of the most requested and inspiring biotech projects has been to make glowing plants. Recently, a group out of Moscow has successfully created a glowing plant that is visible with the naked eye. They were able to do this by using the fungal bioluminescence pathway instead of the bacterial bioluminescence pathway, which increased the efficiency of light production in these eukaryotic organisms.

To learn more, please see the submitted FreeGenes memo, synthesis memos, and other docs:glowing_plant_memo.docx, glowing_plant_synthesis_memo.pdf,

# Documentation

This distribution comprises of {13} genetic parts. {7} of these parts are Luz and Luz alternatives, the main light producing enzyme. {3} of these parts are 2 enzymes that complete the biosynthetic pathway necessary to sustain light production. 1 enzyme, HispS, has been split into 2 parts because it was too large for synthesis directly. It can be put back together with BglII or BsaI with the overhang sequence of GATC. The remaining {3} parts are vector components to put test these genes quickly while a FreeGenes Plant toolkit is put together.

Full history of modifications are available in the snapgene files in sequence_files.zip. Genbank files are also provided in genbank_files.zip. The exact sequences ordered from Twist are available in glowing_plant_order.csv. The genes are flanked by experimentally validated orthogonal primer pairs, and a I-SceI barcode. Full set of primer pairs for all FreeGenes parts are in a git repo. Detailed information on changes can be found in the curators_notes.md. The plate map can be found here.

Quality control documentation is also attached <todo>.

# IP Rights

Planta has filed a provisional patent for use of Luz in plants, and so materials are provided as research use only.

Genes

Gene	Name
Luz	Fungal Luciferase
Luz-Homologue	Fungal Luciferase-Homologue
Luz-Mc	Mycena citricolor Fungal Luciferase
Luz-Oo	Omphalotus olearius Fungal Luciferase
Luz-RFP	Fungal Luciferase RFP fusion
Luz-Y189F	Fungal Luciferase Y189F pocket mutation
Luz-Y233F	Fungal Luciferase Y233F pocket mutation
H3H	hispidin-3-hydroxylase
HispS_1	hispidin synthase
HispS_2	hispidin synthase
Vector-Luz	Luz test vector
Vector-HispS	HispS test vector
Vector-H3H	H3H test vector

MTA

Unilateral OpenMTA

NOTICE OF CONDITIONS

Upon acceptance and use of this Material, the Recipient agrees to the following conditions:

Use: The Recipient shall use, store, and dispose of the Material and any Modifications in accordance with good laboratory practice and shall ensure compliance with all applicable laws, rules, and regulations, including laws, rules, and regulations governing export control and safety.
Attribution: The Recipient has no obligation to acknowledge the Provider
Distribution: The Recipient may distribute the Material and substances created by the Recipient through use of the Material, including Progeny, Unmodified Derivatives, and Modifications, without requesting consent from the Provider. The Provider DISCLAIMS ALL RIGHTS of notification regarding any distribution of the Material by the Recipient to third parties.
Fees: The Material is provided at no cost, or with an optional transmittal fee solely to reimburse the Provider for its preparation and distribution costs.
Intellectual Property: The Provider disclaims all intellectual and other property rights to the supplied Materials. Should Recipient use the Materials to develop novel products or processes, the Provider will not at any time claim downstream rights to these products or processes, or to any subsequent innovations that flow from them.
No Implied License: Except for the rights expressly granted herein, the Recipient agrees that no other rights or licenses, whether express or implied, are granted to the Recipient under any patent, patent application, or other proprietary right of the Provider. As between the parties, each retains all right, title, and interest in works and inventions made by its personnel, and nothing herein shall be construed to transfer ownership of any invention, patent, patent application, or other proprietary right.
Liability: Except to the extent prohibited by law, the Recipient assumes all liability for damages that may arise from the use, storage or disposal of the Material. The Provider will not be liable to the Recipient for any loss, claim or demand made by the Recipient, or made against the Recipient by any other party, due to or arising from the use of the Material by the Recipient, except to the extent permitted by law when caused by the gross negligence or willful misconduct of the Provider.
No Warranties: Any Material delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. THE PROVIDER MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE MATERIAL WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS.

DEFINITIONS:

Material: Original Material, Progeny, and Unmodified Derivatives. The Material shall not include: (a) Modifications, or (b) other substances created by the Recipient through the use of the Material, which are not Modifications, Progeny, or Unmodified Derivatives.
Progeny: Unmodified descendant from the Material, such as virus from virus, cell from cell, or organism from organism.
Unmodified Derivatives: Substances created by the Recipient which constitute an unmodified functional subunit or product expressed by the Original Material. Some examples include: subclones of unmodified cell lines, purified or fractionated subsets of the Original Material, proteins expressed by DNA/RNA supplied by Provider, or monoclonal antibodies secreted by a hybridoma cell line.
Modifications: Substances created by the Recipient which contain/incorporate the Material.