Glowing Plant

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A 3 enzyme pathway coding for fungal bioluminescence, codon optimized for Nicotiana benthamiana in MoClo-compatible high-copy ampicillin resistance backbones.

One of the most requested and inspiring biotech projects has been to make glowing plants. Recently, a group out of Moscow has successfully created a glowing plant that is visible with the naked eye. They were able to do this by using the fungal bioluminescence pathway instead of the bacterial bioluminescence pathway, which increased the efficiency of light production in these eukaryotic organisms.

To learn more, please see the submitted FreeGenes memo, synthesis memos, and other docs:glowing_plant_memo.docx, glowing_plant_synthesis_memo.pdf,

Documentation

This distribution comprises of {13} genetic parts. {7} of these parts are Luz and Luz alternatives, the main light producing enzyme. {3} of these parts are 2 enzymes that complete the biosynthetic pathway necessary to sustain light production. 1 enzyme, HispS, has been split into 2 parts because it was too large for synthesis directly. It can be put back together with BglII or BsaI with the overhang sequence of GATC. The remaining {3} parts are vector components to put test these genes quickly while a FreeGenes Plant toolkit is put together.

The plate map can be found here.

Full history of modifications are available in the snapgene files in sequence_files.zip. Genbank files are also provided in genbank_files.zip. The exact sequences ordered from Twist are available in glowing_plant_order.csv. The genes are flanked by experimentally validated orthogonal primer pairs, and a I-SceI barcode. Full set of primer pairs for all FreeGenes parts are in a git repo. Detailed information on changes can be found in the curators_notes.md.

This product is made available under the unilateral OpenMTA.

Genes

Gene	Name
Luz	Fungal Luciferase
Luz-Homologue	Fungal Luciferase-Homologue
Luz-Mc	Mycena citricolor Fungal Luciferase
Luz-Oo	Omphalotus olearius Fungal Luciferase
Luz-RFP	Fungal Luciferase RFP fusion
Luz-Y189F	Fungal Luciferase Y189F pocket mutation
Luz-Y233F	Fungal Luciferase Y233F pocket mutation
H3H	hispidin-3-hydroxylase
HispS_1	hispidin synthase
HispS_2	hispidin synthase
Vector-Luz	Luz test vector
Vector-HispS	HispS test vector
Vector-H3H	H3H test vector

MTA

Unilateral OpenMTA

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