Glowing Plant

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A 3 enzyme pathway coding for fungal bioluminescence, codon optimized for Nicotiana benthamiana in MoClo-compatible high-copy ampicillin resistance backbones.

One of the most requested and inspiring biotech projects has been to make glowing plants. Recently, a group out of Moscow has successfully created a glowing plant that is visible with the naked eye. They were able to do this by using the fungal bioluminescence pathway instead of the bacterial bioluminescence pathway, which increased the efficiency of light production in these eukaryotic organisms.

To learn more, please see the submitted FreeGenes memo, synthesis memos, and other docs:glowing_plant_memo.docx, glowing_plant_synthesis_memo.pdf,

Documentation

This distribution comprises of {13} genetic parts. {7} of these parts are Luz and Luz alternatives, the main light producing enzyme. {3} of these parts are 2 enzymes that complete the biosynthetic pathway necessary to sustain light production. 1 enzyme, HispS, has been split into 2 parts because it was too large for synthesis directly. It can be put back together with BglII or BsaI with the overhang sequence of GATC. The remaining {3} parts are vector components to put test these genes quickly while a FreeGenes Plant toolkit is put together.

Shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation. The plate map can be found here.

Full history of modifications are available in the snapgene files in sequence_files.zip. Genbank files are also provided in genbank_files.zip. The exact sequences ordered from Twist are available in glowing_plant_order.csv. The genes are flanked by experimentally validated orthogonal primer pairs, and a I-SceI barcode. Full set of primer pairs for all FreeGenes parts are in a git repo. Detailed information on changes can be found in the curators_notes.md.

Note: Partial plasmid duplication has been found for BBF10K_003492 (H3H test vector). This partial duplication should not impact transformation, outgrowth or primer attachment.

This product is made available under the unilateral OpenMTA.

Some or all of these items are for use only as permitted by a research exemption.

Genes

Gene	Name	Freegenes ID
Luz	Fungal Luciferase	BBF10K_003480
Luz-Homologue	Fungal Luciferase-Homologue	BBF10K_003481
Luz-Mc	Mycena citricolor Fungal Luciferase	BBF10K_003482
Luz-Oo	Omphalotus olearius Fungal Luciferase	BBF10K_003483
Luz-RFP	Fungal Luciferase RFP fusion	BBF10K_003484
Luz-Y189F	Fungal Luciferase Y189F pocket mutation	BBF10K_003485
Luz-Y233F	Fungal Luciferase Y233F pocket mutation	BBF10K_003486
H3H	hispidin-3-hydroxylase	BBF10K_003487
HispS_1	hispidin synthase	BBF10K_003488
HispS_2	hispidin synthase	BBF10K_003489
Vector-Luz	Luz test vector	BBF10K_003490
Vector-HispS	HispS test vector	BBF10K_003491
Vector-H3H	H3H test vector	BBF10K_003492

Download all of this information as a CSV from our GitHub.

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name	Contact	Country
Jordan Gonzalez	jgonzalez {at} thecitizensciencelab {dot} org	United States
Travis Wertz	travis {at} traviswertz {dot} com	United States
Erman ÖZER	aracnoid {at} gmail {dot} com	Turkey
Jehovani Lopez	NA	United States
Wei-Min Chang	WeiMinChang {at} tmu {dot} edu {dot} tw	Taiwan
Amjed Alsultan	amjed.talib {at} qu {dot} edu {dot} iq	Iraq
Peer Schenk	reuben.brown {at} uqconnect {dot} edu {dot} au	Australia
Ben Daniels	bndaniel {at} calpoly {dot} edu	United States
Dong Nguyen Tam	NA	Vietnam
Cihan Aydin	cihan.aydin {at} medeniyet {dot} edu {dot} tr	Turkey
Ery Fukushima	ery.fukushima {at} ikiam {dot} edu {dot} ec	Ecuador
Kitty Martens	kitmartens {at} gmail {dot} com	Canada
Hernán Rebolledo	lenaranjo {at} gmail {dot} com	United States
James Altamirano	James_altam {at} yahoo {dot} com	United States
Ahmed Hegazy	a.hegazy {at} biotec {dot} rwth-aachen {dot} de	Germany
Mikhail Khvochtchev	mikhail.khv {at} mahidol {dot} edu	Thailand
Ahmed Atef	ahmed_atefaig2 {at} yahoo {dot} com	United States
Eric Theis	Aracaifon {at} gmail {dot} com	United States
Majed Alghamdi	mafa2 {at} le {dot} ac {dot} uk	United Kingdom
Foong-Jing Goh	wind1989 {at} hotmail {dot} com	Taiwan
Sreenivas Eadara	sreeni.eadara {at} gmail {dot} com	United States
Tomasz Jurkowski	jurkowskit {at} cardiff {dot} ac {dot} uk	United Kingdom

Download all of this information as a CSV from our GitHub.

MTA

Unilateral OpenMTA

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Upon acceptance and use of this Material, the Recipient agrees to the following conditions:

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Material: Original Material, Progeny, and Unmodified Derivatives. The Material shall not include: (a) Modifications, or (b) other substances created by the Recipient through the use of the Material, which are not Modifications, Progeny, or Unmodified Derivatives.
Progeny: Unmodified descendant from the Material, such as virus from virus, cell from cell, or organism from organism.
Unmodified Derivatives: Substances created by the Recipient which constitute an unmodified functional subunit or product expressed by the Original Material. Some examples include: subclones of unmodified cell lines, purified or fractionated subsets of the Original Material, proteins expressed by DNA/RNA supplied by Provider, or monoclonal antibodies secreted by a hybridoma cell line.
Modifications: Substances created by the Recipient which contain/incorporate the Material.