Glowing Plant

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A 3 enzyme pathway coding for fungal bioluminescence, codon optimized for Nicotiana benthamiana in MoClo-compatible high-copy ampicillin resistance backbones.

One of the most requested and inspiring biotech projects has been to make glowing plants. Recently, a group out of Moscow has successfully created a glowing plant that is visible with the naked eye. They were able to do this by using the fungal bioluminescence pathway instead of the bacterial bioluminescence pathway, which increased the efficiency of light production in these eukaryotic organisms.

To learn more, please see the submitted FreeGenes memo, synthesis memos, and other docs:glowing_plant_memo.docx, glowing_plant_synthesis_memo.pdf,

Documentation

This distribution comprises of {13} genetic parts. {7} of these parts are Luz and Luz alternatives, the main light producing enzyme. {3} of these parts are 2 enzymes that complete the biosynthetic pathway necessary to sustain light production. 1 enzyme, HispS, has been split into 2 parts because it was too large for synthesis directly. It can be put back together with BglII or BsaI with the overhang sequence of GATC. The remaining {3} parts are vector components to put test these genes quickly while a FreeGenes Plant toolkit is put together.

All genes are present on the plate in E. coli glycerol stocks. The plate map can be found here.

Full history of modifications are available in the snapgene files in sequence_files.zip. Genbank files are also provided in genbank_files.zip. The exact sequences ordered from Twist are available in glowing_plant_order.csv. The genes are flanked by experimentally validated orthogonal primer pairs, and a I-SceI barcode. Full set of primer pairs for all FreeGenes parts are in a git repo. Detailed information on changes can be found in the curators_notes.md.

This product is made available under the unilateral OpenMTA.

Some or all of these items are for use only as permitted by a research exemption.

Genes

Gene	Name	Freegenes ID
Luz	Fungal Luciferase	BBF10K_003480
Luz-Homologue	Fungal Luciferase-Homologue	BBF10K_003481
Luz-Mc	Mycena citricolor Fungal Luciferase	BBF10K_003482
Luz-Oo	Omphalotus olearius Fungal Luciferase	BBF10K_003483
Luz-RFP	Fungal Luciferase RFP fusion	BBF10K_003484
Luz-Y189F	Fungal Luciferase Y189F pocket mutation	BBF10K_003485
Luz-Y233F	Fungal Luciferase Y233F pocket mutation	BBF10K_003486
H3H	hispidin-3-hydroxylase	BBF10K_003487
HispS_1	hispidin synthase	BBF10K_003488
HispS_2	hispidin synthase	BBF10K_003489
Vector-Luz	Luz test vector	BBF10K_003490
Vector-HispS	HispS test vector	BBF10K_003491
Vector-H3H	H3H test vector	BBF10K_003492

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name	Contact	Country
Amjed Alsultan	amjed.talib {at} qu {dot} edu {dot} iq	Iraq
Ahmed Hegazy	a.hegazy {at} biotec {dot} rwth-aachen {dot} de	Germany
Ben Daniels	bndaniel {at} calpoly {dot} edu	United States
Jordan Gonzalez	jgonzalez {at} thecitizensciencelab {dot} org	United States
Travis Wertz	travis {at} traviswertz {dot} com	United States
James Altamirano	James_altam {at} yahoo {dot} com	United States
Dong Nguyen Tam	NA	Vietnam
Ahmed Atef	ahmed_atefaig2 {at} yahoo {dot} com	United States
Eric Theis	Aracaifon {at} gmail {dot} com	United States

MTA

Unilateral OpenMTA

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