COVID-19 is currently destroying hundreds of thousands of lives around the world. Widespread testing for this virus is necessary to control its spread, but in many places, conducting tests costs too much money because of the reagent cost. We have collected expression plasmids for the most expensive test components, the enzymes. With these expression plasmids, users can produce the enzymes needed to do testing locally much cheaper than it would cost to purchase or import them, which we hope will allow for more COVID-19 tests.
What is it:
This is a collection of 5 different protein-coding genes in expression vectors, including 2 reverse transcriptases (HIV-RT and MMLV-RT), two DNA polymerases (Taq and Bst-LF), and one RNA inhibitor. All vectors, except HIV-RT, are ampicillin resistant, with HIV-RT being kanamycin resistant. The RNAse inhibitor plasmid and the Taq polymerase plasmid both use the tac promoter, while the HIV-RT, MMLV-RT, and Bst-LF plasmids use the T7 promoter.
Shipped as purified and dried down DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
Plate map can be found here.
What can it be used for:
These enzymes can generally be used for reverse transcription and PCR or LAMP amplification. Any protocol that requires any of those 3 reactions can use the enzymes expressed in these vectors.
Thanks to:
These materials were created by the Dickinson lab at UC Chicago, Alex Brown at National Jewish Health, John Schloendorn at Gene And Cell Technology, and the Open Bioeconomy Lab at Cambridge University.
Where I can find more information:
- pOpenTaq Protocol
- HIV-RT Protocol
- MMLV-RT Protocol
- Bst-LF Protocol
- RNAse Inhibitor Protocol
- Open Bioeconomy Lab (for troubleshooting MMLV-RT and Bst-LF)
- Certification Data
Here are the genbank format files for each detection enzyme expression vector. You will need the free SnapGene® viewer or other program.
- Genbank file for HIV-RT expression plasmid
- Genbank file for RNAse Inhibitor expression plasmid
- Genbank file for Bst-LF expression plasmid
- Genbank file for MMLV-RT expression plasmid
- Genbank file for Taq expression plasmid
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
The items in this collection can also be obtained from Addgene as individual items:
- Taq polymerase
- RNAse Inhibitor
- Bst Large Fragment
- MMLV Reverse Transcriptase
- HIV Reverse Transcriptase
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| Taq | pOpenTaq | BBF10K_003493 |
| HIV-RT | His-tagged HIV-RT p66 | BBF10K_003494 |
| MMLV-RT | His-tagged MMLV-RT | BBF10K_003495 |
| Bst-LF | Bst-LF DNA polymerase | BBF10K_003496 |
| RNAse Inhibitor | RNAse Inhibitor | BBF10K_003497 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Kyle Erlenbeck | krerlenbeck {at} dcsdk12 {dot} org | United States |
| Louise Dalgaard | ltd {at} ruc {dot} dk | Denmark |
| Tania Pozzo | tania.pozzo {at} gmail {dot} com | Sweden |
| Jianxiang Shi | jianxiangshi {at} zzu {dot} edu {dot} cn | China |
| Ery Fukushima | ery.fukushima {at} ikiam {dot} edu {dot} ec | Ecuador |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Deepak Saini | deepaksaini {at} iisc {dot} ac {dot} in | India |
| Ferdinand Molnár | NA | Kazakhstan |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Wilson Teran | wteran.doc {at} gmail {dot} com | Colombia |
| Jimmy Gledson Hayden Linhares | haydenlinhars {at} gmail {dot} com | Brazil |
| Leandro Oliveira | leandro.licursi {at} ufv {dot} br | United Kingdom |
| Amjed Alsultan | amjed.talib {at} qu {dot} edu {dot} iq | Iraq |
| Dayananda Chandrappa | nanda.daya {at} gmail {dot} com | India |
| Josephine Labos | N/A | Philippines |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
| elwi machado | elwi.machado {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| Benjamin Loang | benjamin.liang {at} uq {dot} edu {dot} au | Australia |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
| Vitaliy Strochkov | NA | Kazakhstan |
| Norhan Hassan | norhan.hassan {at} kaust {dot} edu {dot} sa | Saudi Arabia |
| irfan hussain | irfan {at} bs {dot} qau {dot} edu {dot} pk | Pakistan |
| Thooyavan George | www.hetrogenebiotech.com | India |
| Shibichakravarthy Kannan | shibi.kannan {at} gmail {dot} com | India |
| Codruta Ignea | codruta.ignea {at} mcgill {dot} ca | Canada |
| Ingrid Lorena Jaramillo | Lojadel2 {at} gmail {dot} com | Colombia |
| mehmet tardu | mtardu {at} gmail {dot} com | Turkey |
| Christopher Kinyanjui Kariuki | christopherkinyanjui.kariuki {at} kuleuven {dot} be | Belgium |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Intan Taufik | i.taufik {at} sith {dot} itb {dot} ac {dot} id | Indonesia |
| Johan Sosa | johan {at} thefunquantum {dot} com | United States |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| raghav sridhar | raghav {at} cambrianbioworks {dot} com | India |
| Dong Nguyen Tam | NA | Vietnam |
| Sarah Turcotte | turcottes {at} cbsnews {dot} com | United States |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
Download all of this information as a CSV from our GitHub.