COVID-19 Diagnostic Toolkit Enzymes

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COVID-19 is currently destroying hundreds of thousands of lives around the world. Widespread testing for this virus is necessary to control its spread, but in many places, conducting tests costs too much money because of the reagent cost. We have collected expression plasmids for the most expensive test components, the enzymes. With these expression plasmids, users can produce the enzymes needed to do testing locally much cheaper than it would cost to purchase or import them, which we hope will allow for more COVID-19 tests.

What is it:
This is a collection of 5 different protein-coding genes in expression vectors, including 2 reverse transcriptases (HIV-RT and MMLV-RT), two DNA polymerases (Taq and Bst-LF), and one RNA inhibitor. All vectors, except HIV-RT, are ampicillin resistant, with HIV-RT being kanamycin resistant. The RNAse inhibitor plasmid and the Taq polymerase plasmid both use the tac promoter, while the HIV-RT, MMLV-RT, and Bst-LF plasmids use the T7 promoter.

Shipped as purified and dried down DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.

Plate map can be found here.

What can it be used for:
These enzymes can generally be used for reverse transcription and PCR or LAMP amplification. Any protocol that requires any of those 3 reactions can use the enzymes expressed in these vectors.

Thanks to:
These materials were created by the Dickinson lab at UC Chicago, Alex Brown at National Jewish Health, John Schloendorn at Gene And Cell Technology, and the Open Bioeconomy Lab at Cambridge University.

Where I can find more information:

pOpenTaq Protocol
HIV-RT Protocol
MMLV-RT Protocol
Bst-LF Protocol
RNAse Inhibitor Protocol
Open Bioeconomy Lab (for troubleshooting MMLV-RT and Bst-LF)
Certification Data

Here are the genbank format files for each detection enzyme expression vector. You will need the free SnapGene® viewer or other program.

This product is made available under the unilateral OpenMTA.

Some or all of these items are for use only as permitted by a research exemption.

The items in this collection can also be obtained from Addgene as individual items:

Genes

Gene	Name	Freegenes ID
Taq	pOpenTaq	BBF10K_003493
HIV-RT	His-tagged HIV-RT p66	BBF10K_003494
MMLV-RT	His-tagged MMLV-RT	BBF10K_003495
Bst-LF	Bst-LF DNA polymerase	BBF10K_003496
RNAse Inhibitor	RNAse Inhibitor	BBF10K_003497

Download all of this information as a CSV from our GitHub.

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name	Contact	Country
Jordan Gonzalez	jgonzalez {at} thecitizensciencelab {dot} org	United States
Jose David Rosales	jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/	Venezuela
Louise Dalgaard	ltd {at} ruc {dot} dk	Denmark
Amjed Alsultan	amjed.talib {at} qu {dot} edu {dot} iq	Iraq
Deepak Saini	deepaksaini {at} iisc {dot} ac {dot} in	India
Norhan Hassan	norhan.hassan {at} kaust {dot} edu {dot} sa	Saudi Arabia
Kyle Erlenbeck	krerlenbeck {at} dcsdk12 {dot} org	United States
Vitaliy Strochkov	NA	Kazakhstan
Dong Nguyen Tam	NA	Vietnam
Ferdinand Molnár	NA	Kazakhstan
Christopher Kinyanjui Kariuki	christopherkinyanjui.kariuki {at} kuleuven {dot} be	Belgium
Jimmy Gledson Hayden Linhares	haydenlinhars {at} gmail {dot} com	Brazil
Emre Yörük	unalsergen {at} gmail {dot} com	Turkey
Ery Fukushima	ery.fukushima {at} ikiam {dot} edu {dot} ec	Ecuador
Ahmed Atef	ahmed_atefaig2 {at} yahoo {dot} com	Saudi Arabia
Oskar Zeballos	zebsamosk {at} gmail {dot} com	Bolivia
Kenneth Frimpong	ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh	Ghana
Johan Sosa	johan {at} thefunquantum {dot} com	United States
Dayananda Chandrappa	nanda.daya {at} gmail {dot} com	India
Ingrid Lorena Jaramillo	Lojadel2 {at} gmail {dot} com	Colombia
Irfan Hussain	irfan {at} bs {dot} qau {dot} edu {dot} pk	Pakistan
Shibichakravarthy Kannan	shibi.kannan {at} gmail {dot} com	India
Codruta Ignea	codruta.ignea {at} mcgill {dot} ca	Canada
Matthew Phanchana	matthew.pha {at} mahidol {dot} edu	Thailand
Benjamin Loang	benjamin.liang {at} uq {dot} edu {dot} au	Australia
Han Teng Wong	wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg	Singapore
Leandro Oliveira	leandro.licursi {at} ufv {dot} br	United Kingdom
raghav sridhar	raghav {at} cambrianbioworks {dot} com	India
Tania Pozzo	tania.pozzo {at} gmail {dot} com	Sweden
Majed Alghamdi	mafa2 {at} le {dot} ac {dot} uk	United Kingdom
Wilson Teran	wteran.doc {at} gmail {dot} com	Colombia

Download all of this information as a CSV from our GitHub.

MTA

Unilateral OpenMTA

NOTICE OF CONDITIONS

Upon acceptance and use of this Material, the Recipient agrees to the following conditions:

Use: The Recipient shall use, store, and dispose of the Material and any Modifications in accordance with good laboratory practice and shall ensure compliance with all applicable laws, rules, and regulations, including laws, rules, and regulations governing export control and safety.
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