COVID-19 Diagnostic Toolkit Enzymes

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COVID-19 is currently destroying hundreds of thousands of lives around the world. Widespread testing for this virus is necessary to control its spread, but in many places, conducting tests costs too much money because of the reagent cost. We have collected expression plasmids for the most expensive test components, the enzymes. With these expression plasmids, users can produce the enzymes needed to do testing locally much cheaper than it would cost to purchase or import them, which we hope will allow for more COVID-19 tests.

What is it:
This is a collection of 5 different protein-coding genes in expression vectors. We have 2 reverse transcriptases (HIV-RT and MMLV-RT), two DNA polymerases (Taq and Bst-LF), and one RNA inhibitor. All vectors, except HIV-RT, are ampicillin resistant, with HIV-RT being kanamycin resistant. The RNAse inhibitor plasmid and the Taq polymerase plasmid both use the tac promoter, while the HIV-RT, MMLV-RT, and Bst-LF plasmids use the T7 promoter.

Unlike most of the collections that FreeGenes offers, all 5 expression vectors will be shipped as raw DNA. You do not need a -80C freezer to store this kit. If you are asked at checkout whether you possess a -80C freezer, you can still receive this kit if you answer 'no.'

What can it be used for:
These enzymes can generally be used for reverse transcription and PCR or LAMP amplification. Any protocol that requires any of those 3 reactions can use the enzymes expressed in these vectors.

Thanks to:
These materials were created by the Dickinson lab at UC Chicago, Alex Brown at National Jewish Health, John Schloendorn at Gene And Cell Technology, and the Open Bioeconomy Lab at Cambridge University.

Where I can find more information:

pOpenTaq Protocol
HIV-RT Protocol
MMLV-RT Protocol
Bst-LF Protocol
RNAse Inhibitor Protocol
Open Bioeconomy Lab (for troubleshooting MMLV-RT and Bst-LF)
Certification Data

Here are the genbank format files for each detection enzyme expression vector. You will need the free SnapGene® viewer or other program.

This product is made available under the unilateral OpenMTA.

Some or all of these items are for use only as permitted by a research exemption.

The items in this collection can also be obtained from Addgene as individual items:

Genes

Gene	Name	Freegenes ID
Taq	pOpenTaq	BBF10K_003493
HIV-RT	His-tagged HIV-RT p66	BBF10K_003494
MMLV-RT	His-tagged MMLV-RT	BBF10K_003495
Bst-LF	Bst-LF DNA polymerase	BBF10K_003496
RNAse Inhibitor	RNAse Inhibitor	BBF10K_003497

Download all of this information as a CSV from our GitHub.

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name	Contact	Country
Norhan Hassan	norhan.hassan {at} kaust {dot} edu {dot} sa	Saudi Arabia
Amjed Alsultan	amjed.talib {at} qu {dot} edu {dot} iq	Iraq
Ingrid Lorena Jaramillo	Lojadel2 {at} gmail {dot} com	Colombia
Vitaliy Strochkov	NA	Kazakhstan
Kenneth Frimpong	ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh	Ghana
Tania Pozzo	tania.pozzo {at} gmail {dot} com	Sweden
Wilson Teran	wteran.doc {at} gmail {dot} com	Colombia
Louise Dalgaard	ltd {at} ruc {dot} dk	Denmark
Ahmed Atef	ahmed_atefaig2 {at} yahoo {dot} com	Saudi Arabia
Dong Nguyen Tam	NA	Vietnam
Christopher Kinyanjui Kariuki	christopherkinyanjui.kariuki {at} kuleuven {dot} be	Belgium
Leandro Oliveira	leandro.licursi {at} ufv {dot} br	United Kingdom
Oskar Zeballos	zebsamosk {at} gmail {dot} com	Bolivia
Jose David Rosales	jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/	Venezuela
Jordan Gonzalez	jgonzalez {at} thecitizensciencelab {dot} org	United States
Matthew Phanchana	matthew.pha {at} mahidol {dot} edu	Thailand
Emre Yörük	unalsergen {at} gmail {dot} com	Turkey
Han Teng Wong	wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg	Singapore
raghav sridhar	raghav {at} cambrianbioworks {dot} com	India
Deepak Saini	deepaksaini {at} iisc {dot} ac {dot} in	India
Majed Alghamdi	mafa2 {at} le {dot} ac {dot} uk	United Kingdom
Jimmy Gledson Hayden Linhares	haydenlinhars {at} gmail {dot} com	Brazil
Johan Sosa	johan {at} thefunquantum {dot} com	United States
Ferdinand Molnár	NA	Kazakhstan
Ery Fukushima	ery.fukushima {at} ikiam {dot} edu {dot} ec	Ecuador
Dayananda Chandrappa	nanda.daya {at} gmail {dot} com	India

Download all of this information as a CSV from our GitHub.

MTA

Unilateral OpenMTA

NOTICE OF CONDITIONS

Upon acceptance and use of this Material, the Recipient agrees to the following conditions:

Use: The Recipient shall use, store, and dispose of the Material and any Modifications in accordance with good laboratory practice and shall ensure compliance with all applicable laws, rules, and regulations, including laws, rules, and regulations governing export control and safety.
Attribution: The Recipient has no obligation to acknowledge the Provider
Distribution: The Recipient may distribute the Material and substances created by the Recipient through use of the Material, including Progeny, Unmodified Derivatives, and Modifications, without requesting consent from the Provider. The Provider DISCLAIMS ALL RIGHTS of notification regarding any distribution of the Material by the Recipient to third parties.
Fees: The Material is provided at no cost, or with an optional transmittal fee solely to reimburse the Provider for its preparation and distribution costs.
Intellectual Property: The Provider disclaims all intellectual and other property rights to the supplied Materials. Should Recipient use the Materials to develop novel products or processes, the Provider will not at any time claim downstream rights to these products or processes, or to any subsequent innovations that flow from them.
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DEFINITIONS:

Material: Original Material, Progeny, and Unmodified Derivatives. The Material shall not include: (a) Modifications, or (b) other substances created by the Recipient through the use of the Material, which are not Modifications, Progeny, or Unmodified Derivatives.
Progeny: Unmodified descendant from the Material, such as virus from virus, cell from cell, or organism from organism.
Unmodified Derivatives: Substances created by the Recipient which constitute an unmodified functional subunit or product expressed by the Original Material. Some examples include: subclones of unmodified cell lines, purified or fractionated subsets of the Original Material, proteins expressed by DNA/RNA supplied by Provider, or monoclonal antibodies secreted by a hybridoma cell line.
Modifications: Substances created by the Recipient which contain/incorporate the Material.