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B. subtilis Essential Genes (E. coli codon optimized)

B. subtilis Essential Genes (E. coli codon optimized)

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261 essential genes from Bacillus subtilis, codon optimized for Escherichia coli in MoClo-compatible high-copy ampicillin resistance backbones.

Discovery of genes essential for life is the first step in creating a truly synthetic organism. The Bacillus subtilis community has done an amazing job in curating a list of essential genes, and while the first published list goes back to 2003, the Bacillus subtilis community has continuously updated this list with results from new experiments. In 2016, a paper was published using a CRISPR-based method for knocking down essential genes in Bacillus subtilis. All the sgRNAs are available through the Bacillus Genetic Stock Center, which would allow for knockdown of native-sequence essential genes. We took the list of essential genes from that 2016 paper and synthesized 261 of the 289 genes through Twist Biosciences.

All the essential genes have been recorded for expression in Escherichia coli and have been made MoClo compatible. There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Bacillus subtilis genome using these genes and the sgRNAs available from the Bacillus Genetic Stock Center. 

Recoding was done using scripts that stochastically assigned codons to amino acids. The codon table used was the FreqB table from "Design Parameters to Control Synthetic Gene Expression in Escherichia coli", which was used because it was shown to express proteins well and was recommended to us by JCVI. All genes were inserted into the BbsI:BbsI cloning sites of pOpen_build, which is individually available through our website.

261 of the 289 essential genes from 2016 essential gene list are included in this distribution. The essential genes included in the distribution at attached in essential_included.csv, while the essential genes not included are in essential_not_included.csv. The spreadsheet BBF_to_uniprot.csv contains links from our genes to outside resources like uniprot, as well as detailed descriptions. In the bsub_essential.zip file are genbank files for every gene.
Shipped as purified and dried down DNA stained with Cresol Red in three 96-well plates. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
The plate map can be found here.

This product is made available under the unilateral OpenMTA Some or all of these items are for use only as permitted by a research exemption.
261 unique parts

Instructions for Use:

Transform into E. coli Top10 cells or equivalent. Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.

PlateSet: Bsubtilis-essentials-1 Bsubtilis-essentials-2 Bsubtilis-essentials-3


Gene Name NCBI ID Freegenes ID
dnaN DNA polymerase III subunit beta NP_387883.1 BBF10K_000750
gyrB DNA gyrase subunit B NP_387887.1 BBF10K_000751
gyrA DNA gyrase subunit A NP_387888.1 BBF10K_000752
guaB Inosine-5'-monophosphate dehydrogenase NP_387890.1 BBF10K_000753
serS serine--tRNA ligase NP_387894.1 BBF10K_000754
dnaX DNA polymerase III subunit gamma/tau NP_387900.2 BBF10K_000755
holB DNA polymerase III subunit delta NP_387912.1 BBF10K_000756
metS methionine--tRNA ligase NP_387919.1 BBF10K_000757
ipk 4-diphosphocytidyl-2C-methyl-D-erythritolkinase NP_387927.1 BBF10K_000758
glmU bifunctional N-acetylglucosamine-1-phosphateuridyltransferase/glucosamine-1-phosphateacetyltransferase NP_387931.1 BBF10K_000759
prs ribose-phosphate pyrophosphokinase NP_387932.1 BBF10K_000760
pth peptidyl-tRNA hydrolase NP_387934.1 BBF10K_000761
divIC cell division protein DivIC NP_387943.1 BBF10K_000762
tilS tRNA(Ile)-lysidine synthase NP_387948.1 BBF10K_000763
hprT hypoxanthine-guanine phosphoribosyltransferase NP_387949.1 BBF10K_000764
sul dihydropteroate synthase NP_387958.1 BBF10K_000765
folB dihydroneopterin aldolase NP_387959.1 BBF10K_000766
folK 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase NP_387960.1 BBF10K_000767
lysS lysine--tRNA ligase NP_387963.1 BBF10K_000768
ispD 2-D-methyl-D-erythritol 4-phosphatecytidylyltransferase NP_387971.1 BBF10K_000769
ispF 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase NP_387972.1 BBF10K_000770
gltX glutamate--tRNA ligase NP_387973.1 BBF10K_000771
cysS cysteine--tRNA ligase NP_387975.1 BBF10K_000772
secE preprotein translocase subunit SecE NP_387981.1 BBF10K_000773
rplJ 50S ribosomal protein L10 NP_387985.2 BBF10K_000774
rplL 50S ribosomal protein L7/L12 NP_387986.1 BBF10K_000775
rpsL 30S ribosomal protein S12 NP_387991.2 BBF10K_000778
rpsG 30S ribosomal protein S7 NP_387992.2 BBF10K_000779
fusA elongation factor G NP_387993.2 BBF10K_000780
tufA elongation factor Tu NP_387994.1 BBF10K_000781
rpsJ 30S ribosomal protein S10 NP_387996.1 BBF10K_000782
rplC 50S ribosomal protein L3 NP_387997.1 BBF10K_000783
rplD 50S ribosomal protein L4 NP_387998.1 BBF10K_000784
rplB 50S ribosomal protein L2 NP_388000.2 BBF10K_000785
rpsS 30S ribosomal protein S19 NP_388001.1 BBF10K_000786
rpsC 30S ribosomal protein S3 NP_388003.2 BBF10K_000787
rplP 50S ribosomal protein L16 NP_388004.1 BBF10K_000788
rpsQ 30S ribosomal protein S17 NP_388006.1 BBF10K_000789
rplN 50S ribosomal protein L14 NP_388007.1 BBF10K_000790
rplX 50S ribosomal protein L24 NP_388008.1 BBF10K_000791
rplE 50S ribosomal protein L5 NP_388009.1 BBF10K_000792
rpsN 30S ribosomal protein S14 NP_388010.1 BBF10K_000793
rpsH 30S ribosomal protein S8 NP_388011.2 BBF10K_000794
rplF 50S ribosomal protein L6 NP_388012.1 BBF10K_000795
rplR 50S ribosomal protein L18 NP_388013.2 BBF10K_000796
rpsE 30S ribosomal protein S5 NP_388014.1 BBF10K_000797
rpmD 50S ribosomal protein L30 NP_388015.1 BBF10K_000798
secY protein translocase subunit SecY NP_388017.1 BBF10K_000799
adk adenylate kinase NP_388018.1 BBF10K_000800
infA translation initiation factor IF-1 NP_388020.1 BBF10K_000802
rpsM 30S ribosomal protein S13 NP_388022.2 BBF10K_000803
rpsK 30S ribosomal protein S11 NP_388023.1 BBF10K_000804
rpoA DNA-directed RNA polymerase subunit alpha NP_388024.1 BBF10K_000805
rplQ 50S ribosomal protein L17 NP_388025.1 BBF10K_000806
rplM 50S ribosomal protein L13 NP_388030.2 BBF10K_000807
rpsI 30S ribosomal protein S9 NP_388031.1 BBF10K_000808
glmM phosphoglucosamine mutase NP_388058.1 BBF10K_000809
glmS glutamine--fructose-6-phosphateaminotransferase NP_388059.1 BBF10K_000810
ycgG hypothetical protein NP_388192.2 BBF10K_000811
nadE NH(3)-dependent NAD(+) synthetase NP_388195.1 BBF10K_000812
aroK shikimate kinase NP_388197.2 BBF10K_000813
ddl D-alanine--D-alanine ligase NP_388337.1 BBF10K_000814
murF UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase NP_388338.1 BBF10K_000815
acpS holo-[acyl-carrier-protein] synthase NP_388343.1 BBF10K_000816
alrA alanine racemase 1 NP_388345.1 BBF10K_000817
yddT hypothetical protein NP_388391.1 BBF10K_000818
ydiC hypothetical protein NP_388473.1 BBF10K_000819
gcp tRNA threonylcarbamoyladenosine biosynthesisprotein Gcp NP_388475.1 BBF10K_000820
groES chaperonin NP_388483.2 BBF10K_000821
groEL chaperonin NP_388484.1 BBF10K_000822
ydiP BsuMI modification methylase subunit YdiP NP_388488.1 BBF10K_000824
ligA DNA ligase NP_388544.1 BBF10K_000826
gatC Glutamyl-tRNA(Gln) amidotransferase subunit C NP_388549.1 BBF10K_000827
gatA Glutamyl-tRNA(Gln) amidotransferase subunit A NP_388550.1 BBF10K_000828
gatB aspartyl/glutamyl-tRNA amidotransferase subunitB NP_388551.2 BBF10K_000829
yerQ diacylglycerol kinase NP_388554.1 BBF10K_000830
yezG hypothetical protein YP_003097692.1 BBF10K_000831
cspR Putative tRNA (cytidine(34)-2'-O)-methyltransferase NP_388774.2 BBF10K_000833
yhdL anti-sigma-M factor YhdL NP_388832.1 BBF10K_000834
plsC 1-acyl-sn-glycerol-3-phosphate acyltransferase NP_388835.1 BBF10K_000835
prsA foldase protein PrsA NP_388876.1 BBF10K_000836
hemE uroporphyrinogen decarboxylase NP_388893.1 BBF10K_000837
hemH ferrochelatase NP_388894.1 BBF10K_000838
hemY protoporphyrinogen oxidase NP_388895.1 BBF10K_000839
yisB hypothetical protein NP_388947.1 BBF10K_000840
fabF 3-oxoacyl-[acyl-carrier-protein] synthase 2 NP_389016.1 BBF10K_000841
trpS tryptophan--tRNA ligase NP_389024.1 BBF10K_000842
ppnK inorganic polyphosphate/ATP-NAD kinase 1 NP_389043.1 BBF10K_000843
hemD hypothetical protein NP_389105.1 BBF10K_000844
yjzJ hypothetical protein YP_003097711.1 BBF10K_000845
xkdB phage-like element PBSX protein XkdB NP_389134.2 BBF10K_000846
xkdC phage-like element PBSX protein XkdC NP_389135.2 BBF10K_000847
mgtE magnesium transporter MgtE NP_389213.1 BBF10K_000848
patA N-acetyl-LL-diaminopimelate aminotransferase NP_389283.2 BBF10K_000849
dapH 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase NP_389301.2 BBF10K_000850
dapL N-acetyldiaminopimelate deacetylase NP_389302.1 BBF10K_000851
rnjA ribonuclease J 1 NP_389336.1 BBF10K_000852
pdhA pyruvate dehydrogenase E1 component subunitalpha NP_389341.1 BBF10K_000853
ylaN hypothetical protein NP_389367.1 BBF10K_000854
ftsW lipid II flippase FtsW NP_389368.1 BBF10K_000855
coaD phosphopantetheine adenylyltransferase NP_389385.1 BBF10K_000856
ftsL cell division protein FtsL NP_389398.1 BBF10K_000857
pbpB penicillin-binding protein 2B NP_389399.2 BBF10K_000858
murE UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase NP_389401.1 BBF10K_000859
mraY phospho-n-acetylmuramoyl-pentapeptide-transferase NP_389402.2 BBF10K_000860
murD UDP-N-acetylmuramoylalanine--D-glutamate ligase NP_389403.1 BBF10K_000861
murG UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase NP_389405.2 BBF10K_000862
murB UDP-N-acetylenolpyruvoylglucosamine reductase NP_389406.1 BBF10K_000863
divIB cell division protein DivIB NP_389407.1 BBF10K_000864
ftsA cell division protein FtsA NP_389411.2 BBF10K_000865
ftsZ cell division protein FtsZ NP_389412.2 BBF10K_000866
ileS isoleucine--tRNA ligase NP_389426.2 BBF10K_000867
coaBC coenzyme A biosynthesis bifunctional proteinCoaBC NP_389452.1 BBF10K_000869
priA Primosomal protein N' NP_389453.1 BBF10K_000870
yloU hypothetical protein NP_389465.1 BBF10K_000872
plsX phosphate acyltransferase NP_389471.1 BBF10K_000873
fabD malonyl CoA-acyl carrier protein transacylase NP_389472.1 BBF10K_000874
fabG 3-oxoacyl-[acyl-carrier-protein] reductase FabG NP_389473.1 BBF10K_000875
acpP acyl carrier protein NP_389474.1 BBF10K_000876
rnc ribonuclease 3 NP_389475.1 BBF10K_000877
ffh signal recognition particle protein NP_389480.1 BBF10K_000879
rpsP 30S ribosomal protein S16 NP_389481.1 BBF10K_000880
trmD tRNA (guanine-N(1)-)-methyltransferase NP_389485.1 BBF10K_000881
rplS 50S ribosomal protein L19 NP_389486.1 BBF10K_000882
rbgA ribosome biogenesis GTPase A NP_389487.1 BBF10K_000883
rpsB 30S ribosomal protein S2 NP_389531.1 BBF10K_000884
tsf elongation factor Ts NP_389532.1 BBF10K_000885
pyrH uridylate kinase NP_389533.2 BBF10K_000886
frr ribosome-recycling factor NP_389534.1 BBF10K_000887
uppS undecaprenyl pyrophosphate synthase NP_389535.1 BBF10K_000888
cdsA phosphatidate cytidylyltransferase NP_389536.1 BBF10K_000889
dxr 1-deoxy-D-xylulose 5-phosphate reductoisomerase NP_389537.2 BBF10K_000890
proS proline--tRNA ligase NP_389539.1 BBF10K_000891
nusA transcription termination/antiterminationprotein NusA NP_389542.1 BBF10K_000893
infB translation initiation factor IF-2 NP_389545.1 BBF10K_000894
ribC riboflavin biosynthesis protein RibC NP_389549.1 BBF10K_000895
rpsO 30S ribosomal protein S15 NP_389550.1 BBF10K_000896
dapG aspartokinase 1 NP_389558.2 BBF10K_000898
pgsA CDP-diacylglycerol--glycerol-3-phosphate3-phosphatidyltransferase NP_389574.2 BBF10K_000900
ymdA ribonuclease Y NP_389578.1 BBF10K_000901
nrdI ribonucleotide reductase stimulatory proteinNrdI NP_389619.1 BBF10K_000902
nrdF ribonucleoside-diphosphate reductase subunitbeta NP_389621.1 BBF10K_000904
yneF hypothetical protein NP_389674.1 BBF10K_000905
yneS glycerol-3-phosphate acyltransferase NP_389689.1 BBF10K_000906
odhB dihydrolipoyllysine-residue succinyltransferasecomponent of 2-oxoglutarate dehydrogenase complex NP_389818.2 BBF10K_000909
yomL hypothetical protein NP_390015.1 BBF10K_000910
sunI hypothetical protein NP_390032.1 BBF10K_000911
dfrA dihydrofolate reductase NP_390064.1 BBF10K_000912
dnaD DNA replication protein DnaD NP_390116.1 BBF10K_000913
panC pantothenate synthetase NP_390123.1 BBF10K_000914
birA bifunctional protein BirA NP_390125.1 BBF10K_000915
cca CCA-adding enzyme NP_390126.1 BBF10K_000916
dapB 4-hydroxy-tetrahydrodipicolinate reductase NP_390130.1 BBF10K_000917
aroE 3-phosphoshikimate 1-carboxyvinyltransferase NP_390141.1 BBF10K_000918
aroF chorismate synthase NP_390152.3 BBF10K_000919
hepT heptaprenyl diphosphate synthase component 2 NP_390155.1 BBF10K_000920
ubiE demethylmenaquinone methyltransferase NP_390156.1 BBF10K_000921
hepS heptaprenyl diphosphate synthase component 1 NP_390157.1 BBF10K_000922
folE GTP cyclohydrolase 1 NP_390159.1 BBF10K_000923
hbs DNA-binding protein HU 1 NP_390160.1 BBF10K_000924
gpsA glycerol-3-phosphate dehydrogenase NP_390164.1 BBF10K_000925
engA GTPase Der NP_390165.1 BBF10K_000926
cmk cytidylate kinase NP_390170.1 BBF10K_000927
resC cytochrome c biogenesis protein ResC NP_390194.2 BBF10K_000928
scpA segregation and condensation protein A NP_390203.1 BBF10K_000930
ypuF hypothetical protein NP_390204.1 BBF10K_000931
yqjK ribonuclease Z NP_390265.1 BBF10K_000932
dxs 1-deoxy-D-xylulose-5-phosphate synthase NP_390307.1 BBF10K_000933
folD bifunctional protein FolD NP_390311.1 BBF10K_000934
yqhY hypothetical protein NP_390313.2 BBF10K_000935
accC acetyl-CoA carboxylase biotin carboxylasesubunit NP_390314.2 BBF10K_000936
accB acetyl-CoA carboxylase biotin carboxyl carrierprotein subunit NP_390315.1 BBF10K_000937
ispG 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) NP_390386.1 BBF10K_000938
ispH 4-hydroxy-3-methylbut-2-enyl diphosphatereductase NP_390395.2 BBF10K_000939
dnaG DNA primase NP_390400.2 BBF10K_000941
glyS glycine--tRNA ligase beta subunit NP_390404.2 BBF10K_000942
glyQ glycine--tRNA ligase alpha subunit NP_390405.1 BBF10K_000943
era GTPase Era NP_390407.1 BBF10K_000944
holA hypothetical protein NP_390434.1 BBF10K_000945
nadD nicotinate-nucleotide adenylyltransferase NP_390442.1 BBF10K_000946
yqeH hypothetical protein NP_390445.1 BBF10K_000947
yqaE XRE family transcriptional regulator NP_390512.1 BBF10K_000948
alaS alanine--tRNA ligase NP_390618.1 BBF10K_000949
mnmA tRNA-specific 2-thiouridylase MnmA NP_390627.3 BBF10K_000950
aspS aspartate--tRNA ligase NP_390633.1 BBF10K_000951
nifS cysteine desulfurase NifS NP_390666.1 BBF10K_000953
rpmA 50S ribosomal protein L27 NP_390672.1 BBF10K_000954
rplU 50S ribosomal protein L21 NP_390674.1 BBF10K_000955
mreD rod shape-determining protein MreD NP_390679.1 BBF10K_000956
mreC cell shape-determining protein MreC NP_390680.2 BBF10K_000957
mreB rod shape-determining protein MreB NP_390681.2 BBF10K_000958
folC folylpolyglutamate synthase NP_390686.1 BBF10K_000959
hemB delta-aminolevulinic acid dehydratase NP_390691.1 BBF10K_000961
hemC porphobilinogen deaminase NP_390693.1 BBF10K_000962
hemA glutamyl-tRNA reductase NP_390695.1 BBF10K_000963
engB GTP-binding protein EngB NP_390697.1 BBF10K_000964
racE glutamate racemase NP_390717.2 BBF10K_000965
trxA thioredoxin NP_390728.1 BBF10K_000966
pheT phenylalanine--tRNA ligase beta subunit NP_390741.1 BBF10K_000967
pheS phenylalanine--tRNA ligase alpha subunit NP_390742.1 BBF10K_000968
ysgA tRNA/rRNA methyltransferase YsgA NP_390743.1 BBF10K_000969
rplT 50S ribosomal protein L20 NP_390763.1 BBF10K_000970
infC translation initiation factor IF-3 NP_390765.1 BBF10K_000971
dnaI primosomal protein DnaI NP_390776.1 BBF10K_000972
dnaB replication initiation and membrane attachmentprotein NP_390777.1 BBF10K_000973
coaE dephospho-CoA kinase NP_390784.1 BBF10K_000975
accA acetyl-CoA carboxylase carboxyltransferasesubunit alpha NP_390798.1 BBF10K_000976
accD acetyl-CoA carboxylase carboxyltransferasesubunit beta NP_390799.2 BBF10K_000977
rpsD 30S ribosomal protein S4 NP_390844.1 BBF10K_000979
tyrS tyrosine--tRNA ligase 1 NP_390845.1 BBF10K_000980
murC UDP-N-acetylmuramate--L-alanine ligase NP_390857.1 BBF10K_000981
asnB asparagine synthetase 1 NP_390932.1 BBF10K_000983
metK S-adenosylmethionine synthase NP_390933.1 BBF10K_000984
mntA manganese-binding lipoprotein MntA NP_390955.1 BBF10K_000985
menC o-succinylbenzoate synthase NP_390956.1 BBF10K_000986
menE 2-succinylbenzoate-CoA ligase NP_390957.1 BBF10K_000987
menD 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase NP_390960.1 BBF10K_000989
mrpA Na(+)/H(+) antiporter subunit A NP_391038.2 BBF10K_000990
mrpB Na(+)/H(+) antiporter subunit B NP_391039.1 BBF10K_000991
mrpC Na(+)/H(+) antiporter subunit C NP_391040.2 BBF10K_000992
mrpD Na(+)/H(+) antiporter subunit D NP_391041.3 BBF10K_000993
mrpE Na(+)/H(+) antiporter subunit E YP_054591.2 BBF10K_000994
mrpF Na(+)/H(+) antiporter subunit F NP_391043.2 BBF10K_000995
mrpG Na(+)/H(+) antiporter subunit G NP_391044.1 BBF10K_000996
yumC ferredoxin--NADP reductase 2 NP_391091.2 BBF10K_000997
dapF diaminopimelate epimerase NP_391097.1 BBF10K_000998
sufB FeS cluster assembly protein SufB NP_391146.1 BBF10K_000999
iscU NifU-like protein NP_391147.1 BBF10K_001000
sufS cysteine desulfurase NP_391148.1 BBF10K_001001
sufD FeS cluster assembly protein SufD NP_391149.1 BBF10K_001002
sufC ABC transporter ATP-binding protein NP_391150.1 BBF10K_001003
rghRA HTH-type transcriptional repressor RghR NP_391246.1 BBF10K_001004
eno enolase NP_391270.1 BBF10K_001005
pgm 2,3-bisphosphoglycerate-independent phosphoglycerate mutase NP_391271.1 BBF10K_001006
gapA glyceraldehyde-3-phosphate dehydrogenase 1 NP_391274.1 BBF10K_001007
trxB thioredoxin NP_391359.1 BBF10K_001008
prfB peptide chain release factor 2 NP_391409.1 BBF10K_001009
secA protein translocase subunit SecA NP_391410.1 BBF10K_001010
tagO undecaprenyl-phosphate N-acetylglucosaminyl1-phosphate transferase NP_391433.1 BBF10K_001011
tuaB teichuronic acid biosynthesis protein TuaB NP_391440.1 BBF10K_001012
mnaA UDP-N-acetylglucosamine 2-epimerase NP_391446.1 BBF10K_001013
tagH teichoic acids export ATP-binding protein TagH NP_391451.1 BBF10K_001014
tagG teichoic acid translocation permease proteinTagG NP_391452.1 BBF10K_001015
tagF CDP-glycerol:poly NP_391453.1 BBF10K_001016
tagD glycerol-3-phosphate cytidylyltransferase NP_391455.1 BBF10K_001017
tagA N-acetylmannosaminyltransferase NP_391456.1 BBF10K_001018
tagB CDP-glycerol:glycerophosphateglycerophosphotransferase NP_391457.1 BBF10K_001019
mbl MreB-like protein NP_391522.2 BBF10K_001020
murAA UDP-N-acetylglucosamine1-carboxyvinyltransferase 1 NP_391557.1 BBF10K_001021
prfA peptide chain release factor 1 NP_391582.1 BBF10K_001023
pyrG CTP synthase NP_391596.1 BBF10K_001024
rodA rod shape-determining protein RodA NP_391691.1 BBF10K_001025
menA 1,4-dihydroxy-2-naphthoate octaprenyltransferase NP_391728.1 BBF10K_001026
yxlC hypothetical protein NP_391748.1 BBF10K_001027
walK sensor histidine kinase NP_391920.1 BBF10K_001028
walR transcriptional regulator NP_391921.1 BBF10K_001029
dnaC replicative DNA helicase NP_391924.2 BBF10K_001030
ppaC manganese-dependent inorganic pyrophosphatase NP_391935.1 BBF10K_001031
rpsR 30S ribosomal protein S18 NP_391969.2 BBF10K_001032
ssbA single-stranded DNA-binding protein NP_391970.1 BBF10K_001033
rnpA ribonuclease P protein component NP_391985.1 BBF10K_001034

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Name Contact Country
Tri Ngo NA United States
Amjed Alsultan amjed.talib {at} qu {dot} edu {dot} iq Iraq
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org United States
Ery Fukushima ery.fukushima {at} ikiam {dot} edu {dot} ec Ecuador
Juan Vicente Farizano juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar Argentina
Jimmy Linhares Synbioamazonas {at} gmail {dot} com Brazil
Shobha KrupaRani pshobha {at} ccmb {dot} res {dot} in India
Han Teng Wong wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg Singapore
Juan Sebastian Alvarez juan.alvarez1 {at} ucalgary {dot} ca Canada
Dong Nguyen Tam NA Vietnam
Majed Alghamdi mafa2 {at} le {dot} ac {dot} uk United Kingdom

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