The Open Enzyme Collection consists of 81 genes for enzymes that are "workhorses" of molecular biology, including DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases and restriction enzymes. The enzymes are useful in both basic and applied fields as diverse as genomics, diagnostics, biodiversity, synthetic biology, DNA origami, immunology, biochemistry and more.
The foundational technologies enabled by this collection of include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and dNTP synthesis. Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve functionality.
The Open Bioeconomy Lab designed and codon-optimized the Open Enzymes parts for expression in Escherichia coli bacteria. All enzymes are obtained from expired patents or for various reasons are not encumbered by patent rights.
What can the collection be used for?
Researchers can use the collection as the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice and then transforming into E. coli bacteria. This can help reduce lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder to obtain. The enzymes can also be used as a base to improve functionality or add new functions through genetic engineering and directed evolution.
Open Enzymes are not provided in expression vectors. In order to produce an enzyme, you must subclone your gene of interest into an expression vector. Open Enzymes are also compatible with the E. coli Protein Expression Toolkit (all parts have CE overhangs). More information about the collection can be obtained from the Open Bioeconomy Lab.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
Instructions for Use:
Open Enzymes is Shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Cresol Red will not impact plasmid transformation.
Transform into Top10 E. coli or equivalent. Pick a colony and culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.
You may find an expression guide on the Open Bioeconomy Lab website.
The current KOD polymerase gene (Well F1) produces a nonfunctional protein. We are working to replace the polymerase gene with a functional version. Thank you to Tomas Moravec for pointing out the issue!
If you have already received the collection, check out the plate layout.
1 Plate in Set
Prior Kit Versions:
If your plate is labeled "Open Enzymes Part 1" or "openenzymes_1," you can find a plate map here.
If your plate is labeled "Open Enzymes Part 2," you can find a plate map here.
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| 9N7polA | 9°N-7 DNA polymerase | BBF10K_003247 |
| K12polLF | DNA Polymerase I, Large (Klenow) Fragment | BBF10K_003248 |
| K12polLF (exo) | DNA Polymerase I, Large (Klenow) Fragment (3′→5′ exo-) | BBF10K_003249 |
| ECOpolA | E. coli DNA Polymerase I | BBF10K_003250 |
| KTHEAQpol | KlenTaq1 polymerase | BBF10K_003251 |
| KODpol | KOD DNA polymerase | BBF10K_003252 |
| Pwopol | Pwo DNA Polymerase | BBF10K_003254 |
| GBDpol (exo) | Pyrococcus Sp. Heat-Stable (exo–) DNA Polymerase (Deep Vent™ (exo–) DNA Polymerase at NEB) | BBF10K_003255 |
| GBDpol | Pyrococcus Sp. Heat-Stable DNA Polymerase (Deep Vent™ at NEB) | BBF10K_003256 |
| THEAQpolA | Taq DNA Polymerase | BBF10K_003257 |
| Tlipol (exo) | Tli DNA polymerase (exo-) (available from NEB as VentR® (exo-) DNA Polymerase) | BBF10K_003259 |
| TthpolA | Tth DNA polymerase | BBF10K_003260 |
| Bstpol | Bst DNA Polymerase, Full Length | BBF10K_003261 |
| BstpolLF | Bst DNA Polymerase, Large Fragment | BBF10K_003262 |
| BsupolLF | Bsu DNA Polymerase I, Large Fragment | BBF10K_003263 |
| phi29pol | phi29 DNA Polymerase | BBF10K_003264 |
| 9N7polA (CT) | 9°N-7 DNA Polymerase chain terminating (NEB > Therminator DNA Pol) | BBF10K_003265 |
| dbh | Sulfolobus DNA Polymerase IV | BBF10K_003266 |
| T4gene43 | T4 DNA Polymerase | BBF10K_003267 |
| T7gene5 | T7 DNA Polymerase (unmodified) | BBF10K_003268 |
| T5gene122 | T5 DNA Polymerase | BBF10K_003269 |
| T4gene30 | T4-DNA Ligase | BBF10K_003270 |
| ECOligA | E. coli DNA Ligase | BBF10K_003271 |
| HSlig3 | DNA Lligase III | BBF10K_003272 |
| HSlig4 | DNA Ligase IV | BBF10K_003273 |
| 9N7lig | 9 Degrees North DNA Ligase | BBF10K_003274 |
| Pfulig | Pfu DNA Ligase | BBF10K_003275 |
| THEAQlig | Taq DNA Ligase | BBF10K_003276 |
| T4gene63 | T4 RNA Ligase 1 | BBF10K_003277 |
| ALPI | CIP (calf intestinal phosphatase) | BBF10K_003278 |
| RNAse A | RNAse A | BBF10K_003279 |
| RNAse H | RNAse H | BBF10K_003280 |
| EcoRIR | EcoRI restriction enzyme | BBF10K_003281 |
| EcoRIM | EcoRI methyltransferase | BBF10K_003282 |
| PstIR | PstI restriction enzyme | BBF10K_003283 |
| PstIM | PstI methyltransferase | BBF10K_003284 |
| MMLV_RT (mut H) | Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations) | BBF10K_003285 |
| MMLV_RT (lack H) | Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) (SuperScriptII) | BBF10K_003286 |
| TthRT | Thermus thermophilus (Tth) RT | BBF10K_003287 |
| T3RNAp | T3 RNA Polymerase | BBF10K_003289 |
| T7RNAp | T7 RNA Polymerase | BBF10K_003290 |
| SP6RNAp | SP6 RNA Polymerase | BBF10K_003291 |
| Eco31I | Eco31I restriction enzyme | BBF10K_003292 |
| Eco31IA | Eco31IA methyltransferase | BBF10K_003293 |
| Eco31IB | Eco31IB methyltransferase | BBF10K_003294 |
| SapIR | SapI restriction enzyme | BBF10K_003295 |
| SapIM1 | M1.SapI methyltransferase | BBF10K_003296 |
| SapIM2 | M2.SapI methyltransferase | BBF10K_003297 |
| XbaIR | XbaI restriction enzyme | BBF10K_003298 |
| XbaIM | M.XbaI methyltransferase | BBF10K_003299 |
| NotIR (GC) | NotI restriction enzyme | BBF10K_003300 |
| NotIM | M.NotI methyltransferase | BBF10K_003301 |
| SfiIR (cDNA) | SfiI (used to create cDNA libraries) | BBF10K_003302 |
| SfiIM | M.SfiI methyltransferase | BBF10K_003303 |
| EcoRV | EcoRV restriction enzyme | BBF10K_003304 |
| EcoRVM | M.EcoRV methyltransferase | BBF10K_003305 |
| NcoIR | NcoI restriction enzyme | BBF10K_003306 |
| NcoIM | M.NcoI methyltransferase | BBF10K_003307 |
| HindIIIR | HindIII restriction enzyme | BBF10K_003308 |
| HindIIIM | M.HindIII methyltransferase | BBF10K_003309 |
| DpnIR | DpnI restriction enzyme | BBF10K_003310 |
| Bst HF | Bst polymerase, High fidelity | BBF10K_003311 |
| ETSSB | Extreme Thermostable Single-Stranded DNA Binding Protein | BBF10K_003312 |
| T5gene12/13 | T5 Exonuclease | BBF10K_003313 |
| TthligK294R | Tth DNA Ligase K294R | BBF10K_003314 |
| dromedNK | dNK from drosophila | BBF10K_003315 |
| TEV protease | TEV protease | BBF10K_003316 |
| T4 PNK | T4 Polynucleotide Kinase | BBF10K_003317 |
| T4-BGT | T4 Phage β-glucosyltransferase | BBF10K_003318 |
| T7EI | T7EI (T7 Endonuclease I) | BBF10K_003319 |
| T4 PGD | T4 PGD (T4 Endonuclease V) | BBF10K_003320 |
| T4 g32 | T4 gene 32 | BBF10K_003321 |
| P45 | P45 (ArchaeMaxx/PfuTurbo) | BBF10K_003322 |
| BovDNTT | Bovine DNTT | BBF10K_003323 |
| DNA Topoisomerase 1B Vaccinia Virus | DNA Topoisomerase 1B Vaccinia Virus | BBF10K_003324 |
| lambda red operon | lambda red operon (exo, bet, gam) | BBF10K_003325 |
| UDG | Uracil DNA glycosylase | BBF10K_003326 |
| Fpg | Formamidopyrimidine DNA Glycosylase | BBF10K_003327 |
| Endonuclease IV E. coli | Endonuclease IV E. coli | BBF10K_003328 |
| Endonuclease VIII E. coli | Endonuclease VIII E. coli | BBF10K_003329 |
| Exonuclease III E. coli | Exonuclease III E. coli | BBF10K_003330 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Paulina Ejsmont | research.lab {at} cri-paris {dot} org | France |
| Jehovani Lopez | jlopez {at} genspace {dot} org | United States |
| Maan Neamah | maanm.neamah {at} uokufa {dot} edu {dot} iq | Iraq |
| Bronagh Hogan | wolfgang.fischle {at} kaust {dot} edu {dot} sa | Saudi Arabia |
| Jorge Marchand | jmarcha {at} uw {dot} edu | United States |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Alexandr Dorif | dorif11 {at} gmail {dot} com | Moldova, Republic of |
| Marko Komlosh | markokomlosh {at} gmail {dot} com | Serbia |
| Juan Sebastian Alvarez | juan.alvarez1 {at} ucalgary {dot} ca | Canada |
| Ashton Cropp | tacropp {at} vcu {dot} edu | United States |
| Tri Ngo | NA | United States |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Vincent Paris | NA | United States |
| Stijn Tobias de Vries | stijn.vries {at} mpi-marburg {dot} mpg {dot} de | Germany |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Vitaliy Strochkov | NA | Kazakhstan |
| Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
| Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Florian Leiß-Maier | florian.leiss-maier {at} tum {dot} de | Germany |
| Nicholas White | nickk.white {at} gmail {dot} com | United States |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Ferdinand Molnár | NA | Kazakhstan |
| Danielle Pedrolli | danielle.pedrolli {at} unesp {dot} br | Brazil |
| Jennifer Hsieh | j.hsieh {at} kurabiotech {dot} com | Chile |
| Long Nguyen | Lnguyen2 {at} ufl {dot} edu | United States |
| Emin Bursa | NA | United States |
| Muhammad Saad Rehmani | saadrehmani88 {at} hotmail {dot} com | China |
| Fernando Bustos | fernando.bustos {at} genosur {dot} com | Chile |
| Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Ivan Slovokhotov | ivan_slovohotov {at} mail {dot} ru | Russia |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Maira Goytia | mgoytia {at} spelman {dot} edu | United States |
| Leandro Oliveira (NG) | ll637 {at} cam {dot} ac {dot} uk | United Kingdom |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Kheng Oon Low | khengoon {at} nibm {dot} my | Malaysia |
| Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
| Ellen Jorgensen | ejorgensen {at} biotechwithoutborders {dot} org | United States |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| Phil Jensik | NA | United States |
| Deepak Saini | deepaksaini {at} iisc {dot} ac {dot} in | India |
| Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
| Cole LaBonne | colelabonne {at} hotmail {dot} com | United States |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| Codruta Ignea | codruta.ignea {at} mcgill {dot} ca | Canada |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| Keith Gagnon | ktgagnon {at} gmail {dot} com | United States |
| Kristel Alman | kristel.alman {at} ut {dot} ee | Estonia |
| Wapouo Fadanka Stephane | mboalab {at} gmail {dot} com | Republic of Cameroon |
| Dayananda Chandrappa | nanda.daya {at} gmail {dot} com | India |
| Benjamin Loang | benjamin.liang {at} uq {dot} edu {dot} au | Australia |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Stephen Klusza | StephenKlusza {at} clayton {dot} edu | United States |
| Muhammad Shoaib | mshoaib {at} lums {dot} edu {dot} pk | Pakistan |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| Zhiyong Lei | zlei {at} umcutrecht {dot} nl | Netherlands |
| Richard Jones | rpj1234rpj {at} gmail {dot} com | United States |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| David Truong | www.truonglab.com | United States |
| Rodolfo Marcolino | rodolfo.marcolino {at} me {dot} com | Argentina |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
| Ingrid Lorena Jaramillo | Lojadel2 {at} gmail {dot} com | Colombia |
| Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
| Bingbing Wan | wanb {at} sjtu {dot} edu {dot} cn | China |
| Andrei Berasneu | a.berasneu {at} kleverlab {dot} pl | Poland |
| Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
| James Altamirano | James_altam {at} utexas {dot} edu | United States |
| Joao Vitor Dutra Molino | candidomolino {at} gmail {dot} com | United States |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Andrei Berasneu | andreyberesnevson {at} gmail {dot} com | Poland |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | United States |
| Matthew Thompson | matthew.thompson-12 {at} postgrad {dot} manchester {dot} ac {dot} uk | United Kingdom |
| Chris Badenhorst | chris.badenhorst {at} uni-greifswald {dot} de | Germany |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
| Franklin Nobrega | f.nobrega {at} soton {dot} ac {dot} uk | United Kingdom |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Dong Nguyen Tam | NA | Vietnam |
| Norhan Hassan | norhan.hassan {at} kaust {dot} edu {dot} sa | Saudi Arabia |
| Kulvinder Gill | kanwar {at} geneshifters {dot} com | United States |
| Ben Davis | bed58 {at} aber {dot} ac {dot} uk | United Kingdom |
| Julio Bonilla | jabonill {at} espol {dot} edu {dot} ec | Ecuador |
| Adrian Filips | tada {at} specyal {dot} com | Canada |
| Joey Riepsaame | joey.riepsaame {at} path {dot} ox {dot} ac {dot} uk | United Kingdom |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Joel Graff | jgraff {at} mtech {dot} edu | United States |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Sarah Ware | sarah.ware {at} bioblaze {dot} org | United States |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
| semih cagan | semihcgn {at} gmail {dot} com | Turkey |
| Friede van der Berg | 21555230 {at} sun {dot} ac {dot} za | South Africa |
Download all of this information as a CSV from our GitHub.