The Open Reporter Collection includes 32 commonly used reporter genes used in all kinds of biology labs for a variety of techniques including measuring gene expression, diagnostics, verifying successful transformations and more. The collection includes chromoproteins (which produce a colour that is visible to the naked eye), fluorescent proteins (that require excitation by a certain wavelength of light and emit another wavelength), luciferases (which emit bioluminscence) and other enzymes that enable colorimetric detection of a substrate by changing its color, for example horseradish peroxidase which classically turns a chemical substrate bright pink in very common antibody-based assays such as ELISA.
What can the collection be used for?
There are so many uses for reporter genes that parts of this collection will be useful for almost any lab performing cell or molecular biology. We have provided reporters with a variety of spectra, output and maturation times to cater for specific research uses. Importantly, we cover a wide variety of colours that are visible to the naked eye and using fluorescent detection, making this collection useful not only for research but also for education and even bioart. All reporters are off-patent and can also be used commercially, which is good news for small biotech companies.
Open Reporters are not provided in expression vectors. In order to see a color, you must subclone your gene of interest into an expression vector.
More information can be found at the Open Bioeconomy Lab Website. A plate map can be found here.
This kit is included in kit plate 2 of the 2022 iGEM distribution. The plate map for kit plate 2 of the iGEM 2022 distribution can be found here.
This product is made available under the unilateral OpenMTA.
Open Reporters is shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
Prior Kit Versions
If you received an earlier version of this kit labeled "Open Enzymes (Part 2)/Open Reporters, you can find the platemap here.
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| cjBlue/ BioBrick K592011 | cjBlue/ BioBrick K592011 | BBF10K_003331 |
| gfasPurple/gfasCP Biobrick K1033918 | gfasPurple/gfasCP Biobrick K1033918 | BBF10K_003335 |
| meffCP | meffCP | BBF10K_003338 |
| meffCFP | meffCFP | BBF10K_003342 |
| mmilCFP | mmilCFP | BBF10K_003343 |
| meleCFP | meleCFP | BBF10K_003344 |
| efasCFP | efasCFP | BBF10K_003345 |
| aacuGFP1 | aacuGFP1 | BBF10K_003346 |
| aacuGFP2 | aacuGFP2 | BBF10K_003347 |
| afraGFP | afraGFP | BBF10K_003348 |
| amilGFP Biobrick K592010 | amilGFP Biobrick K592010 | BBF10K_003349 |
| eechGFP1 | eechGFP1 | BBF10K_003350 |
| eechGFP2 | eechGFP2 | BBF10K_003351 |
| eechGFP3 | eechGFP3 | BBF10K_003352 |
| efasGFP | efasGFP | BBF10K_003353 |
| gfasGFP | gfasGFP | BBF10K_003354 |
| palmGFP | palmGFP | BBF10K_003355 |
| amilRFP | amilRFP | BBF10K_003357 |
| meffRed/meffRFP Biobricks K592012 | meffRed/meffRFP Biobricks K592012 | BBF10K_003358 |
| meleRFP | meleRFP | BBF10K_003360 |
| scubRFP | scubRFP | BBF10K_003361 |
| eforRed/eforCP Biobrick K592012 | eforRed/eforCP Biobrick K592012 | BBF10K_003362 |
| amajLime/amajCFP/amFP486 BioBrick K1033916 | amajLime/amajCFP/amFP486 BioBrick K1033916 | BBF10K_003364 |
| EiraCFP Biobrick J97000 | EiraCFP Biobrick J97000 | BBF10K_003365 |
| HRP | horseradish peroxidase (HRP) | BBF10K_003367 |
| Firefly Luciferase [Photinus pyralis] (Fluc) | Firefly Luciferase [Photinus pyralis] (Fluc) | BBF10K_003368 |
| Luciferase [Luciola cruciata] (Japanese firefly) | Luciferase [Luciola cruciata] (Japanese firefly) | BBF10K_003369 |
| Renilla luciferase | Renilla luciferase | BBF10K_003370 |
| beta-lactamase | beta-lactamase | BBF10K_003371 |
| CIAP | Calf Intestine Alkaline phosphatase (CIAP) | BBF10K_003372 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Jianxiang Shi | jianxiangshi {at} zzu {dot} edu {dot} cn | China |
| Sarah Ware | sarah.ware {at} bioblaze {dot} org | United States |
| Gülşen Günel | gulsen.gunel {at} ogr {dot} iu {dot} edu {dot} tr | Turkey |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
| Hernán Rebolledo | lenaranjo {at} gmail {dot} com | United States |
| Vitaliy Strochkov | NA | Kazakhstan |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Mark Slabodnick | mmslabodnick {at} knox {dot} edu | United States |
| Emin Bursa | NA | United States |
| Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
| Maira Goytia | mgoytia {at} spelman {dot} edu | United States |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Michael Powers | mpowers9304 {at} gmail {dot} com | United States |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| mehmet tardu | mtardu {at} gmail {dot} com | Turkey |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
| Elena Rosca | erosca {at} ashesi {dot} edu {dot} gh | Ghana |
| Tocvic Meng | Tocvic Meng \ Email: tocvic.meng {at} holocyte {dot} com \ website https://gmexpression {dot} com/ | Australia |
| Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | United States |
| Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Kelly Gallagher | kelly.gallagher {at} oneonta {dot} edu | United States |
| Lisandro Pacheco | lpacheco28 {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| Jose David Rosales | https://www.linkedin.com/in/david-rosales-574a7151/ | United States |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Nadia Odaliz Chamana Chura | nadia.chamana {at} utec {dot} edu {dot} pe | France |
| Moritz Venne | moritz.venne {at} web {dot} de | Germany |
| Amir Kashani | NA | Canada |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Kristel Alman | kristel.alman {at} ut {dot} ee | Estonia |
| SERV0223789 CAROLINE BACQUET | caroline.bacquet {at} ikiam {dot} edu {dot} ec | United States |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Fernan Federici | ffederici {at} bio {dot} puc {dot} cl | Chile |
| Leandro Oliveira (NG) | ll637 {at} cam {dot} ac {dot} uk | United Kingdom |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Simon Hofer | soyouwantalab {at} gmail {dot} com | Austria |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Nikolaus Dietz | nikolaus.dietz {at} unibas {dot} ch | Switzerland |
| Vincent Paris | NA | United States |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| James Altamirano | James_altam {at} utexas {dot} edu | United States |
| Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
| Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
| Arthur Zanetti Nunes Fernandes | fgueiros {at} iq {dot} usp {dot} br | United States |
| Yi Wu | F.Nobrega {at} soton {dot} ac {dot} uk | United Kingdom |
| Anil Challa | akchalla {at} uab {dot} edu | United States |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Lorenzo Zolfanelli | biopunk {at} zolfa {dot} nl | France |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Watanyu Bunsermyos | watanyu.bu {at} ku {dot} th | Thailand |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| Tal Globus | bio {at} talglobus {dot} com | United States |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Jehovani Lopez | jlopez {at} genspace {dot} org | United States |
| Elizabeth Bilsland | bilsland {at} unicamp {dot} br | Brazil |
| Joao Vitor Dutra Molino | candidomolino {at} gmail {dot} com | United States |
| Shawn Lyons | smlyons1 {at} bu {dot} edu | United States |
| Jonathan LeCureux | jlecureu {at} svsuedu | United States |
| Meng Lu | menglu {at} iastate {dot} edu | United States |
| Kevin Correia | kevin {at} kedalionlabs {dot} com | Netherlands |
| Intan Taufik | i.taufik {at} sith {dot} itb {dot} ac {dot} id | Indonesia |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| Elliot Medina | elliot.medina {at} moffitt {dot} org | United States |
| Joey Riepsaame | joey.riepsaame {at} path {dot} ox {dot} ac {dot} uk | United Kingdom |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Carlos Ueira-Vieira | ueira {at} ufu {dot} br | Brazil |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| robson tramontina | robson.tramontina {at} gmail {dot} com | Brazil |
| Dr. Rajashree Patil | rajshree.patil.n {at} gmail {dot} com | India |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| John Allan | john.allan {at} eng {dot} ox {dot} ac {dot} uk | United Kingdom |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| Jorge Marchand | jmarcha {at} uw {dot} edu | United States |
| Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
| Benjamin Loang | benjamin.liang {at} uq {dot} edu {dot} au | Australia |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Emmanuele Severi | emmanuele.severi {at} newcastle {dot} ac {dot} uk | United Kingdom |
| Chris Badenhorst | chris.badenhorst {at} uni-greifswald {dot} de | Germany |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Josephine Labos | N/A | Philippines |
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
Download all of this information as a CSV from our GitHub.