The Open Reporter Collection includes 32 commonly used reporter genes used in all kinds of biology labs for a variety of techniques including measuring gene expression, diagnostics, verifying successful transformations and more. The collection includes chromoproteins (which produce a colour that is visible to the naked eye), fluorescent proteins (that require excitation by a certain wavelength of light and emit another wavelength), luciferases (which emit bioluminscence) and other enzymes that enable colorimetric detection of a substrate by changing its color, for example horseradish peroxidase which classically turns a chemical substrate bright pink in very common antibody-based assays such as ELISA.
What can the collection be used for?
There are so many uses for reporter genes that parts of this collection will be useful for almost any lab performing cell or molecular biology. We have provided reporters with a variety of spectra, output and maturation times to cater for specific research uses. Importantly, we cover a wide variety of colours that are visible to the naked eye and using fluorescent detection, making this collection useful not only for research but also for education and even bioart. All reporters are off-patent and can also be used commercially, which is good news for small biotech companies.
Open Reporters are not provided in expression vectors. In order to see a color, you must subclone your gene of interest into an expression vector.
More information can be found at the Open Bioeconomy Lab Website. A plate map can be found here.
This product is made available under the unilateral OpenMTA.
Open Reporters is shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Cresol Red will not impact plasmid transformation.
Prior Kit Versions
If you received an earlier version of this kit labeled "Open Enzymes (Part 2)/Open Reporters, you can find the platemap here.
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| cjBlue/ BioBrick K592011 | cjBlue/ BioBrick K592011 | BBF10K_003331 |
| gfasPurple/gfasCP Biobrick K1033918 | gfasPurple/gfasCP Biobrick K1033918 | BBF10K_003335 |
| meffCP | meffCP | BBF10K_003338 |
| meffCFP | meffCFP | BBF10K_003342 |
| mmilCFP | mmilCFP | BBF10K_003343 |
| meleCFP | meleCFP | BBF10K_003344 |
| efasCFP | efasCFP | BBF10K_003345 |
| aacuGFP1 | aacuGFP1 | BBF10K_003346 |
| aacuGFP2 | aacuGFP2 | BBF10K_003347 |
| afraGFP | afraGFP | BBF10K_003348 |
| amilGFP Biobrick K592010 | amilGFP Biobrick K592010 | BBF10K_003349 |
| eechGFP1 | eechGFP1 | BBF10K_003350 |
| eechGFP2 | eechGFP2 | BBF10K_003351 |
| eechGFP3 | eechGFP3 | BBF10K_003352 |
| efasGFP | efasGFP | BBF10K_003353 |
| gfasGFP | gfasGFP | BBF10K_003354 |
| palmGFP | palmGFP | BBF10K_003355 |
| amilRFP | amilRFP | BBF10K_003357 |
| meffRed/meffRFP Biobricks K592012 | meffRed/meffRFP Biobricks K592012 | BBF10K_003358 |
| meleRFP | meleRFP | BBF10K_003360 |
| scubRFP | scubRFP | BBF10K_003361 |
| eforRed/eforCP Biobrick K592012 | eforRed/eforCP Biobrick K592012 | BBF10K_003362 |
| amajLime/amajCFP/amFP486 BioBrick K1033916 | amajLime/amajCFP/amFP486 BioBrick K1033916 | BBF10K_003364 |
| EiraCFP Biobrick J97000 | EiraCFP Biobrick J97000 | BBF10K_003365 |
| HRP | horseradish peroxidase (HRP) | BBF10K_003367 |
| Firefly Luciferase [Photinus pyralis] (Fluc) | Firefly Luciferase [Photinus pyralis] (Fluc) | BBF10K_003368 |
| Luciferase [Luciola cruciata] (Japanese firefly) | Luciferase [Luciola cruciata] (Japanese firefly) | BBF10K_003369 |
| Renilla luciferase | Renilla luciferase | BBF10K_003370 |
| beta-lactamase | beta-lactamase | BBF10K_003371 |
| CIAP | Calf Intestine Alkaline phosphatase (CIAP) | BBF10K_003372 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Jehovani Lopez | jlopez {at} genspace {dot} org | United States |
| Chris Badenhorst | chris.badenhorst {at} uni-greifswald {dot} de | Germany |
| Kevin Correia | kevin {at} kedalionlabs {dot} com | Netherlands |
| SERV0223789 CAROLINE BACQUET | caroline.bacquet {at} ikiam {dot} edu {dot} ec | United States |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Jorge Marchand | jmarcha {at} uw {dot} edu | United States |
| Kristel Alman | kristel.alman {at} ut {dot} ee | Estonia |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Emin Bursa | NA | United States |
| Lorenzo Zolfanelli | biopunk {at} zolfa {dot} nl | France |
| Nadia Odaliz Chamana Chura | nadia.chamana {at} utec {dot} edu {dot} pe | France |
| Benjamin Loang | benjamin.liang {at} uq {dot} edu {dot} au | Australia |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
| Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| Yi Wu | F.Nobrega {at} soton {dot} ac {dot} uk | United Kingdom |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| Maira Goytia | mgoytia {at} spelman {dot} edu | United States |
| Leandro Oliveira (NG) | ll637 {at} cam {dot} ac {dot} uk | United Kingdom |
| Fernan Federici | ffederici {at} bio {dot} puc {dot} cl | Chile |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
| Jose David Rosales | https://www.linkedin.com/in/david-rosales-574a7151/ | United States |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Vincent Paris | NA | United States |
| Elliot Medina | elliot.medina {at} moffitt {dot} org | United States |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Hernán Rebolledo | lenaranjo {at} gmail {dot} com | United States |
| Joey Riepsaame | joey.riepsaame {at} path {dot} ox {dot} ac {dot} uk | United Kingdom |
| Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Sarah Ware | sarah.ware {at} bioblaze {dot} org | United States |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| James Altamirano | James_altam {at} utexas {dot} edu | United States |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Joao Vitor Dutra Molino | candidomolino {at} gmail {dot} com | United States |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
| Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Vitaliy Strochkov | NA | Kazakhstan |
| Carlos Ueira-Vieira | ueira {at} ufu {dot} br | Brazil |
| Mark Slabodnick | mmslabodnick {at} knox {dot} edu | United States |
| Dr. Rajashree Patil | rajshree.patil.n {at} gmail {dot} com | India |
Download all of this information as a CSV from our GitHub.