The Open Reporter Collection includes 42 commonly used reporter genes used in all kinds of biology labs for a variety of techniques including measuring gene expression, diagnostics, verifying successful transformations and more. The collection includes chromoproteins (which produce a colour that is visible to the naked eye), fluorescent proteins (that require excitation by a certain wavelength of light and emit another wavelength), luciferases (which emit bioluminscence) and other enzymes that enable colorimetric detection of a substrate by changing its color, for example horseradish peroxidase which classically turns a chemical substrate bright pink in very common antibody-based assays such as ELISA.
What can the collection be used for?
There are so many uses for reporter genes that parts of this collection will be useful for almost any lab performing cell or molecular biology. We have provided reporters with a variety of spectra, output and maturation times to cater for specific research uses. Importantly, we cover a wide variety of colours that are visible to the naked eye and using fluorescent detection, making this collection useful not only for research but also for education and even bioart. All reporters are off-patent and can also be used commercially, which is good news for small biotech companies.
Open Reporters are not provided in expression vectors. In order to see a color, you must subclone your gene of interest into an expression vector.
More information can be found at the Open Bioeconomy Lab Website. A plate map can be found here. All genes are present on the plate in E. coli glycerol stocks.
This product is made available under the unilateral OpenMTA.
Prior Kit Versions
If you received an earlier version of this kit labeled "Open Enzymes (Part 2)/Open Reporters, you can find the platemap here.
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| cjBlue/ BioBrick K592011 | cjBlue/ BioBrick K592011 | BBF10K_003331 |
| spisPink/spisCP Biobrick K1033925 | spisPink/spisCP Biobrick K1033925 | BBF10K_003333 |
| amilCP Biobrick K592009 | amilCP Biobrick K592009 | BBF10K_003334 |
| gfasPurple/gfasCP Biobrick K1033918 | gfasPurple/gfasCP Biobrick K1033918 | BBF10K_003335 |
| ahyaCP | ahyaCP | BBF10K_003336 |
| gdjiCP | gdjiCP | BBF10K_003337 |
| meffCP | meffCP | BBF10K_003338 |
| fuGFP | fuGFP | BBF10K_003339 |
| amilCFP | amilCFP | BBF10K_003340 |
| anobCFP2 | anobCFP2 | BBF10K_003341 |
| meffCFP | meffCFP | BBF10K_003342 |
| mmilCFP | mmilCFP | BBF10K_003343 |
| meleCFP | meleCFP | BBF10K_003344 |
| efasCFP | efasCFP | BBF10K_003345 |
| aacuGFP1 | aacuGFP1 | BBF10K_003346 |
| aacuGFP2 | aacuGFP2 | BBF10K_003347 |
| afraGFP | afraGFP | BBF10K_003348 |
| amilGFP Biobrick K592010 | amilGFP Biobrick K592010 | BBF10K_003349 |
| eechGFP1 | eechGFP1 | BBF10K_003350 |
| eechGFP2 | eechGFP2 | BBF10K_003351 |
| eechGFP3 | eechGFP3 | BBF10K_003352 |
| efasGFP | efasGFP | BBF10K_003353 |
| gfasGFP | gfasGFP | BBF10K_003354 |
| palmGFP | palmGFP | BBF10K_003355 |
| amilRFP | amilRFP | BBF10K_003357 |
| meffRed/meffRFP Biobricks K592012 | meffRed/meffRFP Biobricks K592012 | BBF10K_003358 |
| meleRFP | meleRFP | BBF10K_003360 |
| scubRFP | scubRFP | BBF10K_003361 |
| eforRed/eforCP Biobrick K592012 | eforRed/eforCP Biobrick K592012 | BBF10K_003362 |
| asPink/asFP595/asCP BioBrick K1033933 | asPink/asFP595/asCP BioBrick K1033933 | BBF10K_003363 |
| amajLime/amajCFP/amFP486 BioBrick K1033916 | amajLime/amajCFP/amFP486 BioBrick K1033916 | BBF10K_003364 |
| EiraCFP Biobrick J97000 | EiraCFP Biobrick J97000 | BBF10K_003365 |
| HRP | horseradish peroxidase (HRP) | BBF10K_003367 |
| Firefly Luciferase [Photinus pyralis] (Fluc) | Firefly Luciferase [Photinus pyralis] (Fluc) | BBF10K_003368 |
| Luciferase [Luciola cruciata] (Japanese firefly) | Luciferase [Luciola cruciata] (Japanese firefly) | BBF10K_003369 |
| Renilla luciferase | Renilla luciferase | BBF10K_003370 |
| beta-lactamase | beta-lactamase | BBF10K_003371 |
| CIAP | Calf Intestine Alkaline phosphatase (CIAP) | BBF10K_003372 |
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact |
|---|---|
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org |
| Vitaliy Strochkov | NA |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu |
| Keoni Gandall | koeng101 {at} gmail {dot} com |
| James Altamirano | James_altam {at} utexas {dot} edu |