E. coli Protein Expression Toolkit

Regular price: $0.00
Sale price: $0.00
Regular price
Unit price: per

Free Shipping

Are you looking for an all-in-one toolkit to build up your dream construct to try expressing your enzymes of choice, tailored to your lab’s resources and needs? A toolkit containing different promoters, RBSs, linkers, tags, reporters? This collection might be for you.

A collection of >100 DNA parts designed to be assembled in one-pot reaction using BsaI-based Golden-Gate assembly. DNA parts are MoClo, SEVA, Loop and CIDAR assembly compatible. The collection includes:

3 promoters
7 RBS and insulators
9 purification tags, 3 localization signals
5 cleavage sites, flexible linkers
23 genes of which 5 are reporters and 18 are used in diagnostics
2 destination vectors with pink or blue color screening.

What can it be used for:

Use the DNA parts of the collection to assemble the expression plasmids for the protein of your choice, tailored to the resources present in your lab. The collection enables thousands of combinations for each gene of choice. Full power of the collection can be explored using pipetting robots. The collection is compatible with all the CDSs present in the Open Enzyme collections.

Assembly Scheme

If you've already received the kit, check out the layout for Plate 1 and Plate 2.

This product is made available under the unilateral OpenMTA.

If you are interested in requesting additional parts to be added to this toolkit, complete this request form.

Genes

Gene	Name	NCBI ID
CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902	CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902
CE_sarcGFP	CE_sarcGFP
CE_beta-galactosidase	CE_beta-galactosidase
AF_lacZ pDest	AF_lacZ pDest	N/A
AE_lacZ pDest	AE_lacZ pDest	N/A
AF_high copy (pUC) ori/KanR	AF_high copy (pUC) ori/KanR	N/A
AF_medium copy (pET) ori/KanR	AF_medium copy (pET) ori/KanR	N/A
AB_T7	AB_T7	N/A
AB_T7_lacO	AB_T7_lacO	N/A
AB_pTac	AB_pTac	N/A
BN1_BCD2	BN1_BCD2	N/A
BN1_Riboj54_F1	BN1_Riboj54_F1	N/A
BN1_Riboj_B34	BN1_Riboj_B34	N/A
BN1_BCD12	BN1_BCD12	N/A
BN1_BCD23	BN1_BCD23	N/A
BN1_BCD2_His	BN1_BCD2_His	N/A
BN1_RiboJ_B34_His	BN1_RiboJ_B34_His	N/A
BN1_BCD2_DsbA	BN1_BCD2_DsbA	N/A
BN1_BCD2_OmpT	BN1_BCD2_OmpT	N/A
BN1_BCD2_pelB	BN1_BCD2_pelB	N/A
N1N2_Calm	N1N2_Calm	N/A
N1N2_CBD	N1N2_CBD	N/A
N1N2_Cex	N1N2_Cex	N/A
N1N2_MBP	N1N2_MBP	N/A
N1N2_R5	N1N2_R5	N/A
N1N2_GST	N1N2_GST	N/A
N1N2_CipA	N1N2_CipA	N/A
N1N2_S-Tag	N1N2_S-Tag	N/A
N1N2_GB1	N1N2_GB1	N/A
N1N2_GS flexible	N1N2_GS flexible	N/A
N1N3_GS flexible	N1N3_GS flexible	N/A
N1C_GS flexible	N1C_GS flexible	N/A
N1C_His_TEVcut	N1C_His_TEVcut	N/A
BN2_BCD2_His	BN2_BCD2_His	N/A
BN2_RiboJ_B34	BN2_RiboJ_B34	N/A
BC_B0034	BC_B0034	N/A
BC_B0032	BC_B0032	N/A
BC_RiboJ_RBS64	BC_RiboJ_RBS64	N/A
BC_RiboJ_B34	BC_RiboJ_B34	N/A
BC_BCD23	BC_BCD23	N/A
BC_BCD12	BC_BCD12	N/A
BC_BCD2	BC_BCD2	N/A
N2C_GS flexible	N2C_GS flexible	N/A
N2C_TEVcut	N2C_TEVcut	N/A
BC_BCD2_His_TEVcut	BC_BCD2_His_TEVcut	N/A
BC_BCD2_His_fuGFP_TEVcut	BC_BCD2_His_fuGFP_TEVcut	N/A
N2N3_int_∆I	N2N3_int_∆I
N2N3_SUMO	N2N3_SUMO
N2N3_fuGFP	N2N3_fuGFP
N2N3_AmilCP	N2N3_AmilCP
N2N3_GS flexible	N2N3_GS flexible
N3C_SUMO	N3C_SUMO
N3C_TEV	N3C_TEV
N3C_int_∆I	N3C_int_∆I
N3C_fuGFP	N3C_fuGFP
N3C_GS flexible linker	N3C_GS flexible linker	N/A
CD_OMMLV	CD_OMMLV
CD_HIVRT	CD_HIVRT
CD_Bst-LF	CD_Bst-LF
CD_OpenVent	CD_OpenVent
CD_RNAseInhibitor	CD_RNAseInhibitor
CD_gp32	CD_gp32
CD_BsuLF	CD_BsuLF
CD_UvsY	CD_UvsY
CD_UvsX	CD_UvsX
CD_PBCV-1_ligase	CD_PBCV-1_ligase
CD_HRP	CD_HRP
CD_TEV_protease	CD_TEV_protease
CD_SUMO_protease	CD_SUMO_protease
CD_Calmodulin	CD_Calmodulin
CD_fuGFP	CD_fuGFP
CD_AmilCP	CD_AmilCP
CD_TthRNAseH	CD_TthRNAseH
CD_EcRNAseH	CD_EcRNAseH
DN4_Int_SaltS	DN4_Int_SaltS	N/A
DN4_Int_ThiolS	DN4_Int_ThiolS	N/A
DN4_TEV	DN4_TEV	N/A
DN4_fuGFP	DN4_fuGFP	N/A
DN4_GS flexible linker	DN4_GS flexible linker	N/A
DN5_TEVcut	DN5_TEVcut	N/A
DF_TEVcut_His_Stop_TZ	DF_TEVcut_His_Stop_TZ	N/A
DF_TEVcut_fuGFP_His_Stop_TZ	DF_TEVcut_fuGFP_His_Stop_TZ	N/A
N4N5_Int_ThiolS	N4N5_Int_ThiolS	N/A
N4N5_Int_SaltS	N4N5_Int_SaltS	N/A
N4N5_AmilCP	N4N5_AmilCP	N/A
N4N5_fuGFP	N4N5_fuGFP	N/A
N4N5_GS flexible	N4N5_GS flexible	N/A
N4E_3xStop	N4E_3xStop	N/A
N5E_6H	N5E_6H	N/A
N5E_Calm	N5E_Calm	N/A
N5E_CBD	N5E_CBD	N/A
N5E_Cex	N5E_Cex	N/A
N5E_MBP	N5E_MBP	N/A
N5E_R5	N5E_R5	N/A
N5E_CipA	N5E_CipA	N/A
N5E_3xStop	N5E_3xStop	N/A
EF_ECK9600	EF_ECK9600	N/A
EF_T7term	EF_T7term	N/A
EF_TZ	EF_TZ	N/A
AF_spisPink pDest	AF_spisPink pDest	N/A

Back to Open Bioeconomy Genes, Plasmids, etc.

MTA

Unilateral OpenMTA

NOTICE OF CONDITIONS

Upon acceptance and use of this Material, the Recipient agrees to the following conditions:

Use: The Recipient shall use, store, and dispose of the Material and any Modifications in accordance with good laboratory practice and shall ensure compliance with all applicable laws, rules, and regulations, including laws, rules, and regulations governing export control and safety.
Attribution: The Recipient has no obligation to acknowledge the Provider
Distribution: The Recipient may distribute the Material and substances created by the Recipient through use of the Material, including Progeny, Unmodified Derivatives, and Modifications, without requesting consent from the Provider. The Provider DISCLAIMS ALL RIGHTS of notification regarding any distribution of the Material by the Recipient to third parties.
Fees: The Material is provided at no cost, or with an optional transmittal fee solely to reimburse the Provider for its preparation and distribution costs.
Intellectual Property: The Provider disclaims all intellectual and other property rights to the supplied Materials. Should Recipient use the Materials to develop novel products or processes, the Provider will not at any time claim downstream rights to these products or processes, or to any subsequent innovations that flow from them.
No Implied License: Except for the rights expressly granted herein, the Recipient agrees that no other rights or licenses, whether express or implied, are granted to the Recipient under any patent, patent application, or other proprietary right of the Provider. As between the parties, each retains all right, title, and interest in works and inventions made by its personnel, and nothing herein shall be construed to transfer ownership of any invention, patent, patent application, or other proprietary right.
Liability: Except to the extent prohibited by law, the Recipient assumes all liability for damages that may arise from the use, storage or disposal of the Material. The Provider will not be liable to the Recipient for any loss, claim or demand made by the Recipient, or made against the Recipient by any other party, due to or arising from the use of the Material by the Recipient, except to the extent permitted by law when caused by the gross negligence or willful misconduct of the Provider.
No Warranties: Any Material delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. THE PROVIDER MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE MATERIAL WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS.

DEFINITIONS:

Material: Original Material, Progeny, and Unmodified Derivatives. The Material shall not include: (a) Modifications, or (b) other substances created by the Recipient through the use of the Material, which are not Modifications, Progeny, or Unmodified Derivatives.
Progeny: Unmodified descendant from the Material, such as virus from virus, cell from cell, or organism from organism.
Unmodified Derivatives: Substances created by the Recipient which constitute an unmodified functional subunit or product expressed by the Original Material. Some examples include: subclones of unmodified cell lines, purified or fractionated subsets of the Original Material, proteins expressed by DNA/RNA supplied by Provider, or monoclonal antibodies secreted by a hybridoma cell line.
Modifications: Substances created by the Recipient which contain/incorporate the Material.