
Are you looking for an all-in-one toolkit to build up your dream construct to try expressing your enzymes of choice, tailored to your lab’s resources and needs? A toolkit containing different promoters, RBSs, linkers, tags, reporters? This collection might be for you.
A collection of >100 DNA parts designed to be assembled in one-pot reaction using BsaI-based Golden-Gate assembly. DNA parts are MoClo, SEVA, Loop and CIDAR assembly compatible. The collection includes:
- 3 promoters
- 7 RBS and insulators
- 9 purification tags, 3 localization signals
- 5 cleavage sites, flexible linkers
- 23 genes of which 5 are reporters and 18 are used in diagnostics
- 2 destination vectors with pink or blue color screening.
What can it be used for:
Use the DNA parts of the collection to assemble the expression plasmids for the protein of your choice, tailored to the resources present in your lab. The collection enables thousands of combinations for each gene of choice. Full power of the collection can be explored using pipetting robots. The collection is compatible with all the CDSs present in the Open Enzyme collections.
Assembly Scheme
If you've already received the kit, check out the layout for Plate 1 and Plate 2.
This product is made available under the unilateral OpenMTA.
If you are interested in requesting additional parts to be added to this toolkit, complete this request form.
Genes
Gene | Name | NCBI ID |
---|---|---|
CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | |
CE_sarcGFP | CE_sarcGFP | |
CE_beta-galactosidase | CE_beta-galactosidase | |
AF_lacZ pDest | AF_lacZ pDest | N/A |
AE_lacZ pDest | AE_lacZ pDest | N/A |
AF_high copy (pUC) ori/KanR | AF_high copy (pUC) ori/KanR | N/A |
AF_medium copy (pET) ori/KanR | AF_medium copy (pET) ori/KanR | N/A |
AB_T7 | AB_T7 | N/A |
AB_T7_lacO | AB_T7_lacO | N/A |
AB_pTac | AB_pTac | N/A |
BN1_BCD2 | BN1_BCD2 | N/A |
BN1_Riboj54_F1 | BN1_Riboj54_F1 | N/A |
BN1_Riboj_B34 | BN1_Riboj_B34 | N/A |
BN1_BCD12 | BN1_BCD12 | N/A |
BN1_BCD23 | BN1_BCD23 | N/A |
BN1_BCD2_His | BN1_BCD2_His | N/A |
BN1_RiboJ_B34_His | BN1_RiboJ_B34_His | N/A |
BN1_BCD2_DsbA | BN1_BCD2_DsbA | N/A |
BN1_BCD2_OmpT | BN1_BCD2_OmpT | N/A |
BN1_BCD2_pelB | BN1_BCD2_pelB | N/A |
N1N2_Calm | N1N2_Calm | N/A |
N1N2_CBD | N1N2_CBD | N/A |
N1N2_Cex | N1N2_Cex | N/A |
N1N2_MBP | N1N2_MBP | N/A |
N1N2_R5 | N1N2_R5 | N/A |
N1N2_GST | N1N2_GST | N/A |
N1N2_CipA | N1N2_CipA | N/A |
N1N2_S-Tag | N1N2_S-Tag | N/A |
N1N2_GB1 | N1N2_GB1 | N/A |
N1N2_GS flexible | N1N2_GS flexible | N/A |
N1N3_GS flexible | N1N3_GS flexible | N/A |
N1C_GS flexible | N1C_GS flexible | N/A |
N1C_His_TEVcut | N1C_His_TEVcut | N/A |
BN2_BCD2_His | BN2_BCD2_His | N/A |
BN2_RiboJ_B34 | BN2_RiboJ_B34 | N/A |
BC_B0034 | BC_B0034 | N/A |
BC_B0032 | BC_B0032 | N/A |
BC_RiboJ_RBS64 | BC_RiboJ_RBS64 | N/A |
BC_RiboJ_B34 | BC_RiboJ_B34 | N/A |
BC_BCD23 | BC_BCD23 | N/A |
BC_BCD12 | BC_BCD12 | N/A |
BC_BCD2 | BC_BCD2 | N/A |
N2C_GS flexible | N2C_GS flexible | N/A |
N2C_TEVcut | N2C_TEVcut | N/A |
BC_BCD2_His_TEVcut | BC_BCD2_His_TEVcut | N/A |
BC_BCD2_His_fuGFP_TEVcut | BC_BCD2_His_fuGFP_TEVcut | N/A |
N2N3_int_∆I | N2N3_int_∆I | |
N2N3_SUMO | N2N3_SUMO | |
N2N3_fuGFP | N2N3_fuGFP | |
N2N3_AmilCP | N2N3_AmilCP | |
N2N3_GS flexible | N2N3_GS flexible | |
N3C_SUMO | N3C_SUMO | |
N3C_TEV | N3C_TEV | |
N3C_int_∆I | N3C_int_∆I | |
N3C_fuGFP | N3C_fuGFP | |
N3C_GS flexible linker | N3C_GS flexible linker | N/A |
CD_OMMLV | CD_OMMLV | |
CD_HIVRT | CD_HIVRT | |
CD_Bst-LF | CD_Bst-LF | |
CD_OpenVent | CD_OpenVent | |
CD_RNAseInhibitor | CD_RNAseInhibitor | |
CD_gp32 | CD_gp32 | |
CD_BsuLF | CD_BsuLF | |
CD_UvsY | CD_UvsY | |
CD_UvsX | CD_UvsX | |
CD_PBCV-1_ligase | CD_PBCV-1_ligase | |
CD_HRP | CD_HRP | |
CD_TEV_protease | CD_TEV_protease | |
CD_SUMO_protease | CD_SUMO_protease | |
CD_Calmodulin | CD_Calmodulin | |
CD_fuGFP | CD_fuGFP | |
CD_AmilCP | CD_AmilCP | |
CD_TthRNAseH | CD_TthRNAseH | |
CD_EcRNAseH | CD_EcRNAseH | |
DN4_Int_SaltS | DN4_Int_SaltS | N/A |
DN4_Int_ThiolS | DN4_Int_ThiolS | N/A |
DN4_TEV | DN4_TEV | N/A |
DN4_fuGFP | DN4_fuGFP | N/A |
DN4_GS flexible linker | DN4_GS flexible linker | N/A |
DN5_TEVcut | DN5_TEVcut | N/A |
DF_TEVcut_His_Stop_TZ | DF_TEVcut_His_Stop_TZ | N/A |
DF_TEVcut_fuGFP_His_Stop_TZ | DF_TEVcut_fuGFP_His_Stop_TZ | N/A |
N4N5_Int_ThiolS | N4N5_Int_ThiolS | N/A |
N4N5_Int_SaltS | N4N5_Int_SaltS | N/A |
N4N5_AmilCP | N4N5_AmilCP | N/A |
N4N5_fuGFP | N4N5_fuGFP | N/A |
N4N5_GS flexible | N4N5_GS flexible | N/A |
N4E_3xStop | N4E_3xStop | N/A |
N5E_6H | N5E_6H | N/A |
N5E_Calm | N5E_Calm | N/A |
N5E_CBD | N5E_CBD | N/A |
N5E_Cex | N5E_Cex | N/A |
N5E_MBP | N5E_MBP | N/A |
N5E_R5 | N5E_R5 | N/A |
N5E_CipA | N5E_CipA | N/A |
N5E_3xStop | N5E_3xStop | N/A |
EF_ECK9600 | EF_ECK9600 | N/A |
EF_T7term | EF_T7term | N/A |
EF_TZ | EF_TZ | N/A |
AF_spisPink pDest | AF_spisPink pDest | N/A |