Open Yeast Collection

Currently, there are no truly free open source synthetic biology toolkits for engineering yeast. This inhibits innovation and restricts access to these powerful organisms as platforms for biotechnology. The Open Yeast Collection is a free and open DNA toolkit for engineering Saccharomyces cerevisiae and Pichia pastoris, enabling anyone to have a complete and robust toolkit to build new metabolic pathways or produce enzymes and proteins of interest.

Saccharomyces cerevisiae (brewers yeast) is one of the most useful microorganisms to humans with usage in brewing beer, making wine, and baking bread. In addition to these traditional uses, this yeast has been instrumental in biotechnology, being one of the primary model organisms used for industrial fermentation of bioengineered metabolic pathways. Pichia pastoris (Komagataella pastoris) is a species of methylotrophic yeast is frequently used as an expression system for the production of heterologous proteins. 

What can it be used for?

The Open Yeast Collection is a foundational and enabling framework for contributing to the creation of an open, sustainable and equitable bioeconomy. More specifically Open Yeast Collection permits the building of plasmids from free, reusable and redistributable genetic parts for genetically modifying Saccharomyces cerevisiae or for the production of heterologous proteins in Pichia. The plasmids can be used for basic education and research (e.g. protein-protein interactions via yeast-2-hybrid) or for building multi-enzyme metabolic pathways to create a wide variety of natural/commodity chemicals as well as fine and specialized chemicals such as pharmaceuticals. This will appeal to a wide variety of users from educators & students, community-based & academic researchers and bio-entrepreneurs.

What is included?

The Open Yeast Collection contains one (1) 96-well plate holding 92 DNA parts as raw plasmid DNA, each in the standard FreeGenes level 0 pOpen-v3 plasmid and are all AmpR.

19 Yeast Promoters

14 CDS (genes), 2 secretion tags, 1 nuclear localization signal

9 Yeast Terminators

8 Yeast Selection Markers

7 Pairs of 5' and 3' homology regions for genome integration

3 Yeast Origins and 1 Origin of Transfer

14 Left and Right Assembly Connectors for level 2 multi-gene assembly

2 E. coli selection merkers and 2 origins of replication

3 Assembly bridges for optional bypassing of assembly positions.

Where I can find more information?

Open Biofoundry  is the new home for information on the Open Yeast Collection. Watch and contribute to make the collection flourish.

The Reagent Collaboration Network Forum hosts scientists from across the world seeking to support each other in accessing reagents for research and diagnostics, through sharing and partnership.

Open Yeast Collection memo

John Dueber's Yeast Toolkit, released under the restrictive UBMTA, inspired the Open Yeast Collection. The two toolkits use different assembly standards but understanding YTK helps you to understand OYC.

A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly 

This product is designed by Scott Pownall, PhD, with assistance from Isaac Larkin, PhD, on the Pichia parts. 

These parts are made available under the unilateral OpenMTA 

The Open Yeast Collection contains one (1) 96-well plate holding 92 DNA parts as raw plasmid DNA.

Plate map is found: here.

This kit is included in kit plate 2 of the 2022 iGEM distribution. The plate map for kit plate 2 of the iGEM 2022 distribution can be found here. 

Unilateral OpenMTA

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