
Are you looking for an all-in-one toolkit to build up your dream construct to try expressing your enzymes of choice, tailored to your lab’s resources and needs? A toolkit containing different promoters, RBSs, linkers, tags, reporters? This collection might be for you.
A collection of >100 DNA parts designed to be assembled in one-pot reaction using BsaI-based Golden-Gate assembly. DNA parts are MoClo, SEVA, Loop and CIDAR assembly compatible. The collection includes:
- 3 promoters
- 7 RBS and insulators
- 9 purification tags, 3 localization signals
- 5 cleavage sites, flexible linkers
- 23 genes of which 5 are reporters and 18 are used in diagnostics
- 3 destination vectors
The Protein Expression Toolkit is shipped as purified and dried down plasmid DNA stained with Cresol Red in two 96-well plates. Cresol Red will not impact plasmid transformation.
What can it be used for:
Use the DNA parts of the collection to assemble the expression plasmids for the protein of your choice, tailored to the resources present in your lab. The collection enables thousands of combinations for each gene of choice. Full power of the collection can be explored using pipetting robots. The collection is compatible with all the CDSs present in the Open Enzyme collections.
Assembly Scheme
If you've already received the kit, check out the layout for Plate 1 and Plate 2.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
If you are interested in requesting additional parts to be added to this toolkit, complete this request form.
Genes
Gene | Name | NCBI ID | Freegenes ID |
---|---|---|---|
CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | BBF10K_003332 | |
CE_sarcGFP | CE_sarcGFP | BBF10K_003356 | |
CE_beta-galactosidase | CE_beta-galactosidase | BBF10K_003366 | |
AF_lacZ pDest | AF_lacZ pDest | N/A | BBF10K_003373 |
AE_lacZ pDest | AE_lacZ pDest | N/A | BBF10K_003375 |
AF_high copy (pUC) ori/KanR | AF_high copy (pUC) ori/KanR | N/A | BBF10K_003376 |
AF_medium copy (pET) ori/KanR | AF_medium copy (pET) ori/KanR | N/A | BBF10K_003377 |
AB_T7 | AB_T7 | N/A | BBF10K_003378 |
AB_T7_lacO | AB_T7_lacO | N/A | BBF10K_003379 |
AB_pTac | AB_pTac | N/A | BBF10K_003380 |
BT1_BCD2 | BT1_BCD2 | N/A | BBF10K_003384 |
BT1_Riboj54_F1 | BT1_Riboj54_F1 | N/A | BBF10K_003385 |
BT1_Riboj_B34 | BT1_Riboj_B34 | N/A | BBF10K_003386 |
BT1_BCD12 | BT1_BCD12 | N/A | BBF10K_003387 |
BT1_BCD23 | BT1_BCD23 | N/A | BBF10K_003388 |
BT1_BCD2_His | BT1_BCD2_His | N/A | BBF10K_003389 |
BT1_RiboJ_B34_His | BT1_RiboJ_B34_His | N/A | BBF10K_003390 |
BT1_BCD2_DsbA | BT1_BCD2_DsbA | N/A | BBF10K_003391 |
BT1_BCD2_OmpT | BT1_BCD2_OmpT | N/A | BBF10K_003392 |
BT1_BCD2_pelB | BT1_BCD2_pelB | N/A | BBF10K_003393 |
T1T2_Calm | T1T2_Calm | N/A | BBF10K_003395 |
T1T2_CBD | T1T2_CBD | N/A | BBF10K_003396 |
T1T2_Cex | T1T2_Cex | N/A | BBF10K_003397 |
T1T2_MBP | T1T2_MBP | N/A | BBF10K_003398 |
T1T2_R5 | T1T2_R5 | N/A | BBF10K_003399 |
T1T2_GST | T1T2_GST | N/A | BBF10K_003400 |
T1T2_CipA | T1T2_CipA | N/A | BBF10K_003401 |
T1T2_S-Tag | T1T2_S-Tag | N/A | BBF10K_003402 |
T1T2_GB1 | T1T2_GB1 | N/A | BBF10K_003403 |
T1T2_GS flexible | T1T2_GS flexible | N/A | BBF10K_003404 |
T1T3_GS flexible | T1T3_GS flexible | N/A | BBF10K_003405 |
T1C_GS flexible | T1C_GS flexible | N/A | BBF10K_003406 |
T1C_His_TEVcut | T1C_His_TEVcut | N/A | BBF10K_003407 |
BT2_BCD2_His | BT2_BCD2_His | N/A | BBF10K_003408 |
BT2_RiboJ_B34 | BT2_RiboJ_B34 | N/A | BBF10K_003409 |
BC_B0034 | BC_B0034 | N/A | BBF10K_003410 |
BC_B0032 | BC_B0032 | N/A | BBF10K_003411 |
BC_RiboJ_RBS64 | BC_RiboJ_RBS64 | N/A | BBF10K_003412 |
BC_RiboJ_B34 | BC_RiboJ_B34 | N/A | BBF10K_003413 |
BC_BCD23 | BC_BCD23 | N/A | BBF10K_003414 |
BC_BCD12 | BC_BCD12 | N/A | BBF10K_003415 |
BC_BCD2 | BC_BCD2 | N/A | BBF10K_003416 |
T2C_GS flexible | T2C_GS flexible | N/A | BBF10K_003417 |
T2C_TEVcut | T2C_TEVcut | N/A | BBF10K_003418 |
BC_BCD2_His_TEVcut | BC_BCD2_His_TEVcut | N/A | BBF10K_003419 |
BC_BCD2_His_fuGFP_TEVcut | BC_BCD2_His_fuGFP_TEVcut | N/A | BBF10K_003420 |
T2T3_int_∆I | T2T3_int_∆I | BBF10K_003421 | |
T2T3_SUMO | T2T3_SUMO | BBF10K_003422 | |
T2T3_fuGFP | T2T3_fuGFP | BBF10K_003423 | |
T2T3_AmilCP | T2T3_AmilCP | BBF10K_003424 | |
T2T3_GS flexible | T2T3_GS flexible | BBF10K_003425 | |
T3C_SUMO | T3C_SUMO | BBF10K_003426 | |
T3C_TEV | T3C_TEV | BBF10K_003427 | |
T3C_int_∆I | T3C_int_∆I | BBF10K_003428 | |
T3C_fuGFP | T3C_fuGFP | BBF10K_003429 | |
T3C_GS flexible linker | T3C_GS flexible linker | N/A | BBF10K_003430 |
CD_OMMLV | CD_OMMLV | BBF10K_003431 | |
CD_HIVRT | CD_HIVRT | BBF10K_003432 | |
CD_Bst-LF | CD_Bst-LF | BBF10K_003433 | |
CD_OpenVent | CD_OpenVent | BBF10K_003434 | |
CD_RNAseInhibitor | CD_RNAseInhibitor | BBF10K_003436 | |
CD_gp32 | CD_gp32 | BBF10K_003437 | |
CD_BsuLF | CD_BsuLF | BBF10K_003438 | |
CD_UvsY | CD_UvsY | BBF10K_003439 | |
CD_UvsX | CD_UvsX | BBF10K_003440 | |
CE_T7RNApol_Stop | CE_T7RNApol_Stop | BBF10K_003441 | |
CD_PBCV-1_ligase | CD_PBCV-1_ligase | BBF10K_003442 | |
CD_HRP | CD_HRP | BBF10K_003443 | |
CD_TEV_protease | CD_TEV_protease | BBF10K_003444 | |
CD_SUMO_protease | CD_SUMO_protease | BBF10K_003445 | |
CD_Calmodulin | CD_Calmodulin | BBF10K_003446 | |
CD_fuGFP | CD_fuGFP | BBF10K_003447 | |
CD_AmilCP | CD_AmilCP | BBF10K_003448 | |
CD_TthRNAseH | CD_TthRNAseH | BBF10K_003449 | |
CD_EcRNAseH | CD_EcRNAseH | BBF10K_003450 | |
DT4_Int_SaltS | DT4_Int_SaltS | N/A | BBF10K_003451 |
DT4_Int_ThiolS | DT4_Int_ThiolS | N/A | BBF10K_003452 |
DT4_TEV | DT4_TEV | N/A | BBF10K_003453 |
DT4_fuGFP | DT4_fuGFP | N/A | BBF10K_003454 |
DT4_GS flexible linker | DT4_GS flexible linker | N/A | BBF10K_003455 |
DT5_TEVcut | DT5_TEVcut | N/A | BBF10K_003457 |
DF_TEVcut_His_Stop_TZ | DF_TEVcut_His_Stop_TZ | N/A | BBF10K_003459 |
DF_TEVcut_fuGFP_His_Stop_TZ | DF_TEVcut_fuGFP_His_Stop_TZ | N/A | BBF10K_003460 |
T4T5_Int_ThiolS | T4T5_Int_ThiolS | N/A | BBF10K_003461 |
T4T5_Int_SaltS | T4T5_Int_SaltS | N/A | BBF10K_003462 |
T4T5_AmilCP | T4T5_AmilCP | N/A | BBF10K_003463 |
T4T5_fuGFP | T4T5_fuGFP | N/A | BBF10K_003464 |
T4T5_GS flexible | T4T5_GS flexible | N/A | BBF10K_003465 |
T4E_3xStop | T4E_3xStop | N/A | BBF10K_003466 |
T5E_6H | T5E_6H | N/A | BBF10K_003467 |
T5E_Calm | T5E_Calm | N/A | BBF10K_003468 |
T5E_CBD | T5E_CBD | N/A | BBF10K_003469 |
T5E_Cex | T5E_Cex | N/A | BBF10K_003470 |
T5E_MBP | T5E_MBP | N/A | BBF10K_003471 |
T5E_R5 | T5E_R5 | N/A | BBF10K_003472 |
T5E_CipA | T5E_CipA | N/A | BBF10K_003473 |
T5E_3xStop | T5E_3xStop | N/A | BBF10K_003474 |
EF_ECK9600 | EF_ECK9600 | N/A | BBF10K_003475 |
EF_T7term | EF_T7term | N/A | BBF10K_003476 |
EF_TZ | EF_TZ | N/A | BBF10K_003477 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.Name | Contact | Country |
---|---|---|
John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
Nicholas White | nickk.white {at} gmail {dot} com | United States |
Shivang Joshi | https://www.bacto.bio | United Kingdom |
Ferdinand Molnár | NA | Kazakhstan |
Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
James Altamirano | James_altam {at} yahoo {dot} com | United States |
Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
Jennifer Hsieh | j.hsieh {at} kurabiotech {dot} com | Chile |
Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
Alexandr Dorif | dorif11 {at} gmail {dot} com | Moldova, Republic of |
Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
Stephen Klusza | smklusza {at} gmail {dot} com | United States |
Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
Shu Li | shu {at} petribio {dot} com | United States |
Ery Fukushima | ery.fukushima {at} ikiam {dot} edu {dot} ec | Ecuador |
Emin Bursa | NA | United States |
Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
Stephen Klusza | StephenKlusza {at} clayton {dot} edu | United States |
Muhammad Shoaib | mshoaib {at} lums {dot} edu {dot} pk | Pakistan |
Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
Juan Sebastian Alvarez | juan.alvarez1 {at} ucalgary {dot} ca | Canada |
Dong Nguyen Tam | NA | Vietnam |
Ashton Cropp | tacropp {at} vcu {dot} edu | United States |
Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
Rodrigo Caroca | rcaroca {at} uazuay {dot} edu {dot} ec | Ecuador |
Tri Ngo | NA | United States |
Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
Amjed Alsultan | amjed.talib {at} qu {dot} edu {dot} iq | Iraq |
Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
Jose David Rosales | https://www.linkedin.com/in/david-rosales-574a7151/ | United States |
Matias Cabeza | matiascabeza {at} gmail {dot} com | Argentina |
Kheng Oon Low | khengoon {at} nibm {dot} my | Malaysia |
Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
Vincent Paris | NA | United States |
Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
Krishna Gupta | gupta-krishna {at} ipfdd {dot} de | Germany |
Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
TING-WEI LIN | weitinglin66 {at} gmail {dot} com | Taiwan |
Peer Schenk | Reuben.brown {at} uq {dot} edu {dot} au | Australia |
Mariela Escobar | maru {at} michroma {dot} co | Argentina |
Peter Rootes | root0059 {at} umn {dot} edu | United States |
Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
Aileen Button | acbutton {at} ucsd {dot} edu | United States |
Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
Vitaliy Strochkov | NA | Kazakhstan |
semih cagan | semihcgn {at} gmail {dot} com | Turkey |
Andrei Berasneu | andreyberesnevson {at} gmail {dot} com | Poland |
Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
Kyle Erlenbeck | krerlenbeck {at} dcsdk12 {dot} org | United States |
Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
Download all of this information as a CSV from our GitHub.