Are you looking for an all-in-one toolkit to build up your dream construct to try expressing your enzymes of choice, tailored to your lab’s resources and needs? A toolkit containing different promoters, RBSs, linkers, tags, reporters? This collection might be for you.
A collection of >100 DNA parts designed to be assembled in one-pot reaction using BsaI-based Golden-Gate assembly. DNA parts are MoClo, SEVA, Loop and CIDAR assembly compatible. The collection includes:
- 3 promoters
- 7 RBS and insulators
- 9 purification tags, 3 localization signals
- 5 cleavage sites, flexible linkers
- 23 genes of which 5 are reporters and 18 are used in diagnostics
- 3 destination vectors
The Protein Expression Toolkit is shipped as purified and dried down plasmid DNA stained with Cresol Red in two 96-well plates. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
What can it be used for:
Use the DNA parts of the collection to assemble the expression plasmids for the protein of your choice, tailored to the resources present in your lab. The collection enables thousands of combinations for each gene of choice. Full power of the collection can be explored using pipetting robots. The collection is compatible with all the CDSs present in the Open Enzyme collections.
Assembly Scheme
If you've already received the kit, check out the layout for Plate 1 and Plate 2.
This kit is included in kit plate 2 of the 2022 iGEM distribution. The plate map for kit plate 2 of the iGEM 2022 distribution can be found here.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
If you are interested in requesting additional parts to be added to this toolkit, complete this request form.
Genes
| Gene | Name | NCBI ID | Freegenes ID |
|---|---|---|---|
| CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | BBF10K_003332 | |
| CE_sarcGFP | CE_sarcGFP | BBF10K_003356 | |
| CE_beta-galactosidase | CE_beta-galactosidase | BBF10K_003366 | |
| AF_lacZ pDest | AF_lacZ pDest | N/A | BBF10K_003373 |
| AE_lacZ pDest | AE_lacZ pDest | N/A | BBF10K_003375 |
| AF_high copy (pUC) ori/KanR | AF_high copy (pUC) ori/KanR | N/A | BBF10K_003376 |
| AF_medium copy (pET) ori/KanR | AF_medium copy (pET) ori/KanR | N/A | BBF10K_003377 |
| AB_T7 | AB_T7 | N/A | BBF10K_003378 |
| AB_T7_lacO | AB_T7_lacO | N/A | BBF10K_003379 |
| AB_pTac | AB_pTac | N/A | BBF10K_003380 |
| BT1_BCD2 | BT1_BCD2 | N/A | BBF10K_003384 |
| BT1_Riboj54_F1 | BT1_Riboj54_F1 | N/A | BBF10K_003385 |
| BT1_Riboj_B34 | BT1_Riboj_B34 | N/A | BBF10K_003386 |
| BT1_BCD12 | BT1_BCD12 | N/A | BBF10K_003387 |
| BT1_BCD23 | BT1_BCD23 | N/A | BBF10K_003388 |
| BT1_BCD2_His | BT1_BCD2_His | N/A | BBF10K_003389 |
| BT1_RiboJ_B34_His | BT1_RiboJ_B34_His | N/A | BBF10K_003390 |
| BT1_BCD2_DsbA | BT1_BCD2_DsbA | N/A | BBF10K_003391 |
| BT1_BCD2_OmpT | BT1_BCD2_OmpT | N/A | BBF10K_003392 |
| BT1_BCD2_pelB | BT1_BCD2_pelB | N/A | BBF10K_003393 |
| T1T2_Calm | T1T2_Calm | N/A | BBF10K_003395 |
| T1T2_CBD | T1T2_CBD | N/A | BBF10K_003396 |
| T1T2_Cex | T1T2_Cex | N/A | BBF10K_003397 |
| T1T2_MBP | T1T2_MBP | N/A | BBF10K_003398 |
| T1T2_R5 | T1T2_R5 | N/A | BBF10K_003399 |
| T1T2_GST | T1T2_GST | N/A | BBF10K_003400 |
| T1T2_CipA | T1T2_CipA | N/A | BBF10K_003401 |
| T1T2_S-Tag | T1T2_S-Tag | N/A | BBF10K_003402 |
| T1T2_GB1 | T1T2_GB1 | N/A | BBF10K_003403 |
| T1T2_GS flexible | T1T2_GS flexible | N/A | BBF10K_003404 |
| T1T3_GS flexible | T1T3_GS flexible | N/A | BBF10K_003405 |
| T1C_GS flexible | T1C_GS flexible | N/A | BBF10K_003406 |
| T1C_His_TEVcut | T1C_His_TEVcut | N/A | BBF10K_003407 |
| BT2_BCD2_His | BT2_BCD2_His | N/A | BBF10K_003408 |
| BT2_RiboJ_B34 | BT2_RiboJ_B34 | N/A | BBF10K_003409 |
| BC_B0034 | BC_B0034 | N/A | BBF10K_003410 |
| BC_B0032 | BC_B0032 | N/A | BBF10K_003411 |
| BC_RiboJ_RBS64 | BC_RiboJ_RBS64 | N/A | BBF10K_003412 |
| BC_RiboJ_B34 | BC_RiboJ_B34 | N/A | BBF10K_003413 |
| BC_BCD23 | BC_BCD23 | N/A | BBF10K_003414 |
| BC_BCD12 | BC_BCD12 | N/A | BBF10K_003415 |
| BC_BCD2 | BC_BCD2 | N/A | BBF10K_003416 |
| T2C_GS flexible | T2C_GS flexible | N/A | BBF10K_003417 |
| T2C_TEVcut | T2C_TEVcut | N/A | BBF10K_003418 |
| BC_BCD2_His_TEVcut | BC_BCD2_His_TEVcut | N/A | BBF10K_003419 |
| BC_BCD2_His_fuGFP_TEVcut | BC_BCD2_His_fuGFP_TEVcut | N/A | BBF10K_003420 |
| T2T3_int_∆I | T2T3_int_∆I | BBF10K_003421 | |
| T2T3_SUMO | T2T3_SUMO | BBF10K_003422 | |
| T2T3_fuGFP | T2T3_fuGFP | BBF10K_003423 | |
| T2T3_AmilCP | T2T3_AmilCP | BBF10K_003424 | |
| T2T3_GS flexible | T2T3_GS flexible | BBF10K_003425 | |
| T3C_SUMO | T3C_SUMO | BBF10K_003426 | |
| T3C_TEV | T3C_TEV | BBF10K_003427 | |
| T3C_int_∆I | T3C_int_∆I | BBF10K_003428 | |
| T3C_fuGFP | T3C_fuGFP | BBF10K_003429 | |
| T3C_GS flexible linker | T3C_GS flexible linker | N/A | BBF10K_003430 |
| CD_OMMLV | CD_OMMLV | BBF10K_003431 | |
| CD_HIVRT | CD_HIVRT | BBF10K_003432 | |
| CD_Bst-LF | CD_Bst-LF | BBF10K_003433 | |
| CD_OpenVent | CD_OpenVent | BBF10K_003434 | |
| CD_RNAseInhibitor | CD_RNAseInhibitor | BBF10K_003436 | |
| CD_gp32 | CD_gp32 | BBF10K_003437 | |
| CD_BsuLF | CD_BsuLF | BBF10K_003438 | |
| CD_UvsY | CD_UvsY | BBF10K_003439 | |
| CD_UvsX | CD_UvsX | BBF10K_003440 | |
| CE_T7RNApol_Stop | CE_T7RNApol_Stop | BBF10K_003441 | |
| CD_PBCV-1_ligase | CD_PBCV-1_ligase | BBF10K_003442 | |
| CD_HRP | CD_HRP | BBF10K_003443 | |
| CD_TEV_protease | CD_TEV_protease | BBF10K_003444 | |
| CD_SUMO_protease | CD_SUMO_protease | BBF10K_003445 | |
| CD_Calmodulin | CD_Calmodulin | BBF10K_003446 | |
| CD_fuGFP | CD_fuGFP | BBF10K_003447 | |
| CD_AmilCP | CD_AmilCP | BBF10K_003448 | |
| CD_TthRNAseH | CD_TthRNAseH | BBF10K_003449 | |
| CD_EcRNAseH | CD_EcRNAseH | BBF10K_003450 | |
| DT4_Int_SaltS | DT4_Int_SaltS | N/A | BBF10K_003451 |
| DT4_Int_ThiolS | DT4_Int_ThiolS | N/A | BBF10K_003452 |
| DT4_TEV | DT4_TEV | N/A | BBF10K_003453 |
| DT4_fuGFP | DT4_fuGFP | N/A | BBF10K_003454 |
| DT4_GS flexible linker | DT4_GS flexible linker | N/A | BBF10K_003455 |
| DT5_TEVcut | DT5_TEVcut | N/A | BBF10K_003457 |
| DF_TEVcut_His_Stop_TZ | DF_TEVcut_His_Stop_TZ | N/A | BBF10K_003459 |
| DF_TEVcut_fuGFP_His_Stop_TZ | DF_TEVcut_fuGFP_His_Stop_TZ | N/A | BBF10K_003460 |
| T4T5_Int_ThiolS | T4T5_Int_ThiolS | N/A | BBF10K_003461 |
| T4T5_Int_SaltS | T4T5_Int_SaltS | N/A | BBF10K_003462 |
| T4T5_AmilCP | T4T5_AmilCP | N/A | BBF10K_003463 |
| T4T5_fuGFP | T4T5_fuGFP | N/A | BBF10K_003464 |
| T4T5_GS flexible | T4T5_GS flexible | N/A | BBF10K_003465 |
| T4E_3xStop | T4E_3xStop | N/A | BBF10K_003466 |
| T5E_6H | T5E_6H | N/A | BBF10K_003467 |
| T5E_Calm | T5E_Calm | N/A | BBF10K_003468 |
| T5E_CBD | T5E_CBD | N/A | BBF10K_003469 |
| T5E_Cex | T5E_Cex | N/A | BBF10K_003470 |
| T5E_MBP | T5E_MBP | N/A | BBF10K_003471 |
| T5E_R5 | T5E_R5 | N/A | BBF10K_003472 |
| T5E_CipA | T5E_CipA | N/A | BBF10K_003473 |
| T5E_3xStop | T5E_3xStop | N/A | BBF10K_003474 |
| EF_ECK9600 | EF_ECK9600 | N/A | BBF10K_003475 |
| EF_T7term | EF_T7term | N/A | BBF10K_003476 |
| EF_TZ | EF_TZ | N/A | BBF10K_003477 |
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Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Yi Zhang | www.pharmozyme.com | United States |
| Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
| TING-WEI LIN | weitinglin66 {at} gmail {dot} com | Taiwan |
| Gülşen Günel | gulsen.gunel {at} ogr {dot} iu {dot} edu {dot} tr | Turkey |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
| Ferdinand Molnár | NA | Kazakhstan |
| Daniel Fernandez | NA | United States |
| Vitaliy Strochkov | NA | Kazakhstan |
| Stephen Klusza | StephenKlusza {at} clayton {dot} edu | United States |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Ery Fukushima | ery.fukushima {at} ikiam {dot} edu {dot} ec | Ecuador |
| Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
| Andrei Berasneu | andreyberesnevson {at} gmail {dot} com | Poland |
| Emin Bursa | NA | United States |
| Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
| Kyle Erlenbeck | krerlenbeck {at} dcsdk12 {dot} org | United States |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Tocvic Meng | Tocvic Meng \ Email: tocvic.meng {at} holocyte {dot} com \ website https://gmexpression {dot} com/ | Australia |
| Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | United States |
| Ashton Cropp | tacropp {at} vcu {dot} edu | United States |
| Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Kelly Gallagher | kelly.gallagher {at} oneonta {dot} edu | United States |
| Lisandro Pacheco | lpacheco28 {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| elwi machado | elwi.machado {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| Alexandr Dorif | dorif11 {at} gmail {dot} com | Moldova, Republic of |
| Jose David Rosales | https://www.linkedin.com/in/david-rosales-574a7151/ | United States |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Moritz Venne | moritz.venne {at} web {dot} de | Germany |
| Amir Kashani | NA | Canada |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| William Fried | uscigem {at} usc {dot} edu | United States |
| Matias Cabeza | matiascabeza {at} gmail {dot} com | Argentina |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| semih cagan | semihcgn {at} gmail {dot} com | Turkey |
| Simon Hofer | soyouwantalab {at} gmail {dot} com | Austria |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Tri Ngo | NA | United States |
| Nikolaus Dietz | nikolaus.dietz {at} unibas {dot} ch | Switzerland |
| Vincent Paris | NA | United States |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
| Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
| Arthur Zanetti Nunes Fernandes | fgueiros {at} iq {dot} usp {dot} br | United States |
| Marc Juul | marc {at} juul {dot} io | United States |
| Anil Challa | akchalla {at} uab {dot} edu | United States |
| Wapouo Fadanka Stephane | mboalab {at} gmail {dot} com | Republic of Cameroon |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Muzaffar Muminov | muminov.imbio {at} gmail {dot} com | Uzbekistan |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Watanyu Bunsermyos | watanyu.bu {at} ku {dot} th | Thailand |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| Tal Globus | bio {at} talglobus {dot} com | United States |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Elizabeth Bilsland | bilsland {at} unicamp {dot} br | Brazil |
| Jonathan LeCureux | jlecureu {at} svsuedu | United States |
| Shu Li | shu {at} petribio {dot} com | United States |
| Meng Lu | menglu {at} iastate {dot} edu | United States |
| Peer Schenk | Reuben.brown {at} uq {dot} edu {dot} au | Australia |
| Intan Taufik | i.taufik {at} sith {dot} itb {dot} ac {dot} id | Indonesia |
| Andrew Gray | andrewg {at} bioq {dot} org {dot} au | Australia |
| Nick McGregor | mcgregn2 {at} gmail {dot} com | Canada |
| James Altamirano | James_altam {at} yahoo {dot} com | United States |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| Amjed Alsultan | amjed.talib {at} qu {dot} edu {dot} iq | Iraq |
| Jennifer Hsieh | j.hsieh {at} kurabiotech {dot} com | Chile |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| Dayananda Chandrappa | e2ebiotech {at} gmail {dot} com | India |
| Krishna Gupta | gupta-krishna {at} ipfdd {dot} de | Germany |
| Kheng Oon Low | khengoon {at} nibm {dot} my | Malaysia |
| robson tramontina | robson.tramontina {at} gmail {dot} com | Brazil |
| Patrik D'haeseleer | patrikd {at} gmail {dot} com | United States |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| John Allan | john.allan {at} eng {dot} ox {dot} ac {dot} uk | United Kingdom |
| Michael Miller | michael.j.miller {at} uon {dot} edu {dot} au | Australia |
| Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Juan Sebastian Alvarez | juan.alvarez1 {at} ucalgary {dot} ca | Canada |
| Nicholas White | nickk.white {at} gmail {dot} com | United States |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Dong Nguyen Tam | NA | Vietnam |
| Josephine Labos | N/A | Philippines |
| John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Rodrigo Caroca | rcaroca {at} uazuay {dot} edu {dot} ec | Ecuador |
| Muhammad Shoaib | mshoaib {at} lums {dot} edu {dot} pk | Pakistan |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
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