Are you looking for an all-in-one toolkit to build up your dream construct to try expressing your enzymes of choice, tailored to your lab’s resources and needs? A toolkit containing different promoters, RBSs, linkers, tags, reporters? This collection might be for you.
A collection of >100 DNA parts designed to be assembled in one-pot reaction using BsaI-based Golden-Gate assembly. DNA parts are MoClo, SEVA, Loop and CIDAR assembly compatible. The collection includes:
- 3 promoters
- 7 RBS and insulators
- 9 purification tags, 3 localization signals
- 5 cleavage sites, flexible linkers
- 23 genes of which 5 are reporters and 18 are used in diagnostics
- 3 destination vectors
The Protein Expression Toolkit is shipped as purified and dried down plasmid DNA stained with Cresol Red in two 96-well plates. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
What can it be used for:
Use the DNA parts of the collection to assemble the expression plasmids for the protein of your choice, tailored to the resources present in your lab. The collection enables thousands of combinations for each gene of choice. Full power of the collection can be explored using pipetting robots. The collection is compatible with all the CDSs present in the Open Enzyme collections.
Assembly Scheme
If you've already received the kit, check out the layout for Plate 1 and Plate 2.
This kit is included in kit plate 2 of the 2022 iGEM distribution. The plate map for kit plate 2 of the iGEM 2022 distribution can be found here.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
If you are interested in requesting additional parts to be added to this toolkit, complete this request form.
Genes
| Gene | Name | NCBI ID | Freegenes ID |
|---|---|---|---|
| CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | BBF10K_003332 | |
| CE_sarcGFP | CE_sarcGFP | BBF10K_003356 | |
| CE_beta-galactosidase | CE_beta-galactosidase | BBF10K_003366 | |
| AF_lacZ pDest | AF_lacZ pDest | N/A | BBF10K_003373 |
| AE_lacZ pDest | AE_lacZ pDest | N/A | BBF10K_003375 |
| AF_high copy (pUC) ori/KanR | AF_high copy (pUC) ori/KanR | N/A | BBF10K_003376 |
| AF_medium copy (pET) ori/KanR | AF_medium copy (pET) ori/KanR | N/A | BBF10K_003377 |
| AB_T7 | AB_T7 | N/A | BBF10K_003378 |
| AB_T7_lacO | AB_T7_lacO | N/A | BBF10K_003379 |
| AB_pTac | AB_pTac | N/A | BBF10K_003380 |
| BT1_BCD2 | BT1_BCD2 | N/A | BBF10K_003384 |
| BT1_Riboj54_F1 | BT1_Riboj54_F1 | N/A | BBF10K_003385 |
| BT1_Riboj_B34 | BT1_Riboj_B34 | N/A | BBF10K_003386 |
| BT1_BCD12 | BT1_BCD12 | N/A | BBF10K_003387 |
| BT1_BCD23 | BT1_BCD23 | N/A | BBF10K_003388 |
| BT1_BCD2_His | BT1_BCD2_His | N/A | BBF10K_003389 |
| BT1_RiboJ_B34_His | BT1_RiboJ_B34_His | N/A | BBF10K_003390 |
| BT1_BCD2_DsbA | BT1_BCD2_DsbA | N/A | BBF10K_003391 |
| BT1_BCD2_OmpT | BT1_BCD2_OmpT | N/A | BBF10K_003392 |
| BT1_BCD2_pelB | BT1_BCD2_pelB | N/A | BBF10K_003393 |
| T1T2_Calm | T1T2_Calm | N/A | BBF10K_003395 |
| T1T2_CBD | T1T2_CBD | N/A | BBF10K_003396 |
| T1T2_Cex | T1T2_Cex | N/A | BBF10K_003397 |
| T1T2_MBP | T1T2_MBP | N/A | BBF10K_003398 |
| T1T2_R5 | T1T2_R5 | N/A | BBF10K_003399 |
| T1T2_GST | T1T2_GST | N/A | BBF10K_003400 |
| T1T2_CipA | T1T2_CipA | N/A | BBF10K_003401 |
| T1T2_S-Tag | T1T2_S-Tag | N/A | BBF10K_003402 |
| T1T2_GB1 | T1T2_GB1 | N/A | BBF10K_003403 |
| T1T2_GS flexible | T1T2_GS flexible | N/A | BBF10K_003404 |
| T1T3_GS flexible | T1T3_GS flexible | N/A | BBF10K_003405 |
| T1C_GS flexible | T1C_GS flexible | N/A | BBF10K_003406 |
| T1C_His_TEVcut | T1C_His_TEVcut | N/A | BBF10K_003407 |
| BT2_BCD2_His | BT2_BCD2_His | N/A | BBF10K_003408 |
| BT2_RiboJ_B34 | BT2_RiboJ_B34 | N/A | BBF10K_003409 |
| BC_B0034 | BC_B0034 | N/A | BBF10K_003410 |
| BC_B0032 | BC_B0032 | N/A | BBF10K_003411 |
| BC_RiboJ_RBS64 | BC_RiboJ_RBS64 | N/A | BBF10K_003412 |
| BC_RiboJ_B34 | BC_RiboJ_B34 | N/A | BBF10K_003413 |
| BC_BCD23 | BC_BCD23 | N/A | BBF10K_003414 |
| BC_BCD12 | BC_BCD12 | N/A | BBF10K_003415 |
| BC_BCD2 | BC_BCD2 | N/A | BBF10K_003416 |
| T2C_GS flexible | T2C_GS flexible | N/A | BBF10K_003417 |
| T2C_TEVcut | T2C_TEVcut | N/A | BBF10K_003418 |
| BC_BCD2_His_TEVcut | BC_BCD2_His_TEVcut | N/A | BBF10K_003419 |
| BC_BCD2_His_fuGFP_TEVcut | BC_BCD2_His_fuGFP_TEVcut | N/A | BBF10K_003420 |
| T2T3_int_∆I | T2T3_int_∆I | BBF10K_003421 | |
| T2T3_SUMO | T2T3_SUMO | BBF10K_003422 | |
| T2T3_fuGFP | T2T3_fuGFP | BBF10K_003423 | |
| T2T3_AmilCP | T2T3_AmilCP | BBF10K_003424 | |
| T2T3_GS flexible | T2T3_GS flexible | BBF10K_003425 | |
| T3C_SUMO | T3C_SUMO | BBF10K_003426 | |
| T3C_TEV | T3C_TEV | BBF10K_003427 | |
| T3C_int_∆I | T3C_int_∆I | BBF10K_003428 | |
| T3C_fuGFP | T3C_fuGFP | BBF10K_003429 | |
| T3C_GS flexible linker | T3C_GS flexible linker | N/A | BBF10K_003430 |
| CD_OMMLV | CD_OMMLV | BBF10K_003431 | |
| CD_HIVRT | CD_HIVRT | BBF10K_003432 | |
| CD_Bst-LF | CD_Bst-LF | BBF10K_003433 | |
| CD_OpenVent | CD_OpenVent | BBF10K_003434 | |
| CD_RNAseInhibitor | CD_RNAseInhibitor | BBF10K_003436 | |
| CD_gp32 | CD_gp32 | BBF10K_003437 | |
| CD_BsuLF | CD_BsuLF | BBF10K_003438 | |
| CD_UvsY | CD_UvsY | BBF10K_003439 | |
| CD_UvsX | CD_UvsX | BBF10K_003440 | |
| CE_T7RNApol_Stop | CE_T7RNApol_Stop | BBF10K_003441 | |
| CD_PBCV-1_ligase | CD_PBCV-1_ligase | BBF10K_003442 | |
| CD_HRP | CD_HRP | BBF10K_003443 | |
| CD_TEV_protease | CD_TEV_protease | BBF10K_003444 | |
| CD_SUMO_protease | CD_SUMO_protease | BBF10K_003445 | |
| CD_Calmodulin | CD_Calmodulin | BBF10K_003446 | |
| CD_fuGFP | CD_fuGFP | BBF10K_003447 | |
| CD_AmilCP | CD_AmilCP | BBF10K_003448 | |
| CD_TthRNAseH | CD_TthRNAseH | BBF10K_003449 | |
| CD_EcRNAseH | CD_EcRNAseH | BBF10K_003450 | |
| DT4_Int_SaltS | DT4_Int_SaltS | N/A | BBF10K_003451 |
| DT4_Int_ThiolS | DT4_Int_ThiolS | N/A | BBF10K_003452 |
| DT4_TEV | DT4_TEV | N/A | BBF10K_003453 |
| DT4_fuGFP | DT4_fuGFP | N/A | BBF10K_003454 |
| DT4_GS flexible linker | DT4_GS flexible linker | N/A | BBF10K_003455 |
| DT5_TEVcut | DT5_TEVcut | N/A | BBF10K_003457 |
| DF_TEVcut_His_Stop_TZ | DF_TEVcut_His_Stop_TZ | N/A | BBF10K_003459 |
| DF_TEVcut_fuGFP_His_Stop_TZ | DF_TEVcut_fuGFP_His_Stop_TZ | N/A | BBF10K_003460 |
| T4T5_Int_ThiolS | T4T5_Int_ThiolS | N/A | BBF10K_003461 |
| T4T5_Int_SaltS | T4T5_Int_SaltS | N/A | BBF10K_003462 |
| T4T5_AmilCP | T4T5_AmilCP | N/A | BBF10K_003463 |
| T4T5_fuGFP | T4T5_fuGFP | N/A | BBF10K_003464 |
| T4T5_GS flexible | T4T5_GS flexible | N/A | BBF10K_003465 |
| T4E_3xStop | T4E_3xStop | N/A | BBF10K_003466 |
| T5E_6H | T5E_6H | N/A | BBF10K_003467 |
| T5E_Calm | T5E_Calm | N/A | BBF10K_003468 |
| T5E_CBD | T5E_CBD | N/A | BBF10K_003469 |
| T5E_Cex | T5E_Cex | N/A | BBF10K_003470 |
| T5E_MBP | T5E_MBP | N/A | BBF10K_003471 |
| T5E_R5 | T5E_R5 | N/A | BBF10K_003472 |
| T5E_CipA | T5E_CipA | N/A | BBF10K_003473 |
| T5E_3xStop | T5E_3xStop | N/A | BBF10K_003474 |
| EF_ECK9600 | EF_ECK9600 | N/A | BBF10K_003475 |
| EF_T7term | EF_T7term | N/A | BBF10K_003476 |
| EF_TZ | EF_TZ | N/A | BBF10K_003477 |
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Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Kelly Gallagher | kelly.gallagher {at} oneonta {dot} edu | United States |
| Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
| Ferdinand Molnár | NA | Kazakhstan |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Emin Bursa | NA | United States |
| James Altamirano | James_altam {at} yahoo {dot} com | United States |
| Kheng Oon Low | khengoon {at} nibm {dot} my | Malaysia |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | United States |
| Anil Challa | akchalla {at} uab {dot} edu | United States |
| Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
| Michael Miller | michael.j.miller {at} uon {dot} edu {dot} au | Australia |
| elwi machado | elwi.machado {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Peer Schenk | Reuben.brown {at} uq {dot} edu {dot} au | Australia |
| Matias Cabeza | matiascabeza {at} gmail {dot} com | Argentina |
| William Fried | uscigem {at} usc {dot} edu | United States |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| John Allan | john.allan {at} eng {dot} ox {dot} ac {dot} uk | United Kingdom |
| Moritz Venne | moritz.venne {at} web {dot} de | Germany |
| Andrei Berasneu | andreyberesnevson {at} gmail {dot} com | Poland |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Gülşen Günel | gulsen.gunel {at} ogr {dot} iu {dot} edu {dot} tr | Turkey |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Lisandro Pacheco | lpacheco28 {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| Ashton Cropp | tacropp {at} vcu {dot} edu | United States |
| Tocvic Meng | Tocvic Meng \ Email: tocvic.meng {at} holocyte {dot} com \ website https://gmexpression {dot} com/ | Australia |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Yi Zhang | www.pharmozyme.com | United States |
| Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| Daniel Fernandez | NA | United States |
| Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
| John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Tri Ngo | NA | United States |
| Dong Nguyen Tam | NA | Vietnam |
| Intan Taufik | i.taufik {at} sith {dot} itb {dot} ac {dot} id | Indonesia |
| Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
| Josephine Labos | N/A | Philippines |
| robson tramontina | robson.tramontina {at} gmail {dot} com | Brazil |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Nikolaus Dietz | nikolaus.dietz {at} unibas {dot} ch | Switzerland |
| Jose David Rosales | https://www.linkedin.com/in/david-rosales-574a7151/ | United States |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Nick McGregor | mcgregn2 {at} gmail {dot} com | Canada |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Alexandr Dorif | dorif11 {at} gmail {dot} com | Moldova, Republic of |
| Andrew Gray | andrewg {at} bioq {dot} org {dot} au | Australia |
| Amir Kashani | NA | Canada |
| Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
| Simon Hofer | soyouwantalab {at} gmail {dot} com | Austria |
| Elizabeth Bilsland | bilsland {at} unicamp {dot} br | Brazil |
| Juan Sebastian Alvarez | juan.alvarez1 {at} ucalgary {dot} ca | Canada |
| Meng Lu | menglu {at} iastate {dot} edu | United States |
| Muhammad Shoaib | mshoaib {at} lums {dot} edu {dot} pk | Pakistan |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| semih cagan | semihcgn {at} gmail {dot} com | Turkey |
| Vincent Paris | NA | United States |
| Stephen Klusza | StephenKlusza {at} clayton {dot} edu | United States |
| Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| Tal Globus | bio {at} talglobus {dot} com | United States |
| Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Ery Fukushima | ery.fukushima {at} ikiam {dot} edu {dot} ec | Ecuador |
| Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Wapouo Fadanka Stephane | mboalab {at} gmail {dot} com | Republic of Cameroon |
| Krishna Gupta | gupta-krishna {at} ipfdd {dot} de | Germany |
| Watanyu Bunsermyos | watanyu.bu {at} ku {dot} th | Thailand |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Kyle Erlenbeck | krerlenbeck {at} dcsdk12 {dot} org | United States |
| Nicholas White | nickk.white {at} gmail {dot} com | United States |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
| Patrik D'haeseleer | patrikd {at} gmail {dot} com | United States |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Amjed Alsultan | amjed.talib {at} qu {dot} edu {dot} iq | Iraq |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| TING-WEI LIN | weitinglin66 {at} gmail {dot} com | Taiwan |
| Marc Juul | marc {at} juul {dot} io | United States |
| Rodrigo Caroca | rcaroca {at} uazuay {dot} edu {dot} ec | Ecuador |
| Jonathan LeCureux | jlecureu {at} svsuedu | United States |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Arthur Zanetti Nunes Fernandes | fgueiros {at} iq {dot} usp {dot} br | United States |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Muzaffar Muminov | muminov.imbio {at} gmail {dot} com | Uzbekistan |
| Dayananda Chandrappa | e2ebiotech {at} gmail {dot} com | India |
| Jennifer Hsieh | j.hsieh {at} kurabiotech {dot} com | Chile |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| Vitaliy Strochkov | NA | Kazakhstan |
| Shu Li | shu {at} petribio {dot} com | United States |
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