Mycoplasma pneumoniae is a close relative to Mycoplasma mycoides and Mycoplasma genitalium, and is known to cause disease in humans. Being able to rapidly test differences between those 3 genomes is necessary for synthetic genome research, and the genes themselves are important for biomedical research to find new drug targets. However, there is no resource for Mycoplasma pneumoniae genes to conduct this research. To enable comparative synthetic genome research and biomedical advancements, FreeGenes has synthesized 189 of 690 Mycoplasma pneumoniae M129 genes, codon optimized for Escherichia coli. Hopefully, by supporting Mycoplasma research, FreeGenes can encourage synthetic biology laboratories to conduct synthetic genome research.
What is it
This is a collection of as many protein coding sequences from Mycoplasma pneumoniae M129 as FreeGenes could clone in a single round of cloning. They are all standardized for MoClo assembly and codon optimized for Escherichia coli.
What can it be used for
There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Mycoplasma genome.
Shipped as purified and dried down plasmid DNA stained with Cresol Red in two 96-well plates. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation. This product is made available under the unilateral OpenMTA.
Where I can find more information
- Uniprot Proteome (https://www.uniprot.org/proteomes/UP000000808)
- KEGG GENOME (https://www.genome.jp/kegg-bin/show_organism?org=mpn)
- Genbank (https://www.ncbi.nlm.nih.gov/nuccore/NC_000912.1)
189 unique parts
Instructions for Use:
Transform into Top10 E. coli or equivalent. Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.
PlateSet: Mpneumonia-0 Mpneumonia-1
Genes
| Gene | Name | NCBI ID | Freegenes ID |
|---|---|---|---|
| xdj1 | DnaJ domain-containing protein | NP_109690.1 | BBF10K_002428 |
| yabD | deoxyribonuclease | NP_109697.1 | BBF10K_002435 |
| MPN013 | hypothetical protein | NP_109701.1 | BBF10K_002439 |
| rpoE | DNA-directed RNA polymerase subunit delta | NP_109712.1 | BBF10K_002450 |
| MPN032 | hypothetical protein | NP_109720.1 | BBF10K_002458 |
| upp | uracil phosphoribosyltransferase | NP_109721.1 | BBF10K_002459 |
| MPN038 | hypothetical protein | NP_109726.1 | BBF10K_002464 |
| glpF | glycerol uptake facilitator | NP_109731.1 | BBF10K_002466 |
| MPN047 | nicotinate phosphoribosyltransferase | NP_109735.1 | BBF10K_002471 |
| glpK | glycerol kinase | NP_109738.1 | BBF10K_002476 |
| MPN054 | hypothetical protein | NP_109742.1 | BBF10K_002480 |
| potB | spermidine/putrescine transport system permease | NP_109744.1 | BBF10K_002482 |
| gcp | tRNA N6-adenosine threonylcarbamoyltransferase | NP_109747.1 | BBF10K_002485 |
| metX | S-adenosylmethionine synthase | NP_109748.1 | BBF10K_002486 |
| deoD | purine nucleoside phosphorylase | NP_109750.1 | BBF10K_002489 |
| deoA | thymidine phosphorylase | NP_109752.1 | BBF10K_002491 |
| yabF | ribonuclease M5 | NP_109760.1 | BBF10K_002499 |
| fruK | 1-phosphofructokinase | NP_109767.1 | BBF10K_002506 |
| MPN083 | lipoprotein | NP_109771.1 | BBF10K_002510 |
| MPN085 | hypothetical protein | NP_109773.1 | BBF10K_002512 |
| MPN087 | hypothetical protein | NP_109775.1 | BBF10K_002514 |
| hsdS | hypothetical protein | NP_109777.1 | BBF10K_002516 |
| MPN094 | hypothetical protein | NP_109782.1 | BBF10K_002518 |
| MPN095 | hypothetical protein | NP_109783.1 | BBF10K_002519 |
| MPN096 | hypothetical protein | NP_109784.1 | BBF10K_002520 |
| MPN101 | hypothetical protein | NP_109789.1 | BBF10K_002522 |
| MPN103 | hypothetical protein | NP_109791.1 | BBF10K_002523 |
| MPN104 | hypothetical protein | NP_109792.1 | BBF10K_002524 |
| pheS | phenylalanine--tRNA ligase subunit alpha | NP_109793.1 | BBF10K_002526 |
| MPN110 | hypothetical protein | NP_109798.1 | BBF10K_002531 |
| MPN112 | hypothetical protein | NP_109800.1 | BBF10K_002533 |
| infC | translation initiation factor IF-3 | NP_109803.1 | BBF10K_002535 |
| MPN126 | metallophosphoesterase | NP_109814.1 | BBF10K_002546 |
| MPN129 | hypothetical protein | NP_109817.1 | BBF10K_002547 |
| MPN130 | hypothetical protein | NP_109818.1 | BBF10K_002548 |
| ugpC | ABC transporter ATP-binding protein | NP_109822.1 | BBF10K_002552 |
| ugpE | ABC transporter permease | NP_109824.1 | BBF10K_002555 |
| MPN138 | hypothetical protein | NP_109826.1 | BBF10K_002557 |
| MPN145 | hypothetical protein | NP_109833.1 | BBF10K_002564 |
| rbfA | ribosome-binding factor A | NP_109844.1 | BBF10K_002575 |
| yaaC | bifunctional riboflavin kinase/FMNadenylyltransferase | NP_109846.1 | BBF10K_002577 |
| MPN160 | hypothetical protein | NP_109848.1 | BBF10K_002579 |
| MPN162 | hypothetical protein | NP_109850.1 | BBF10K_002581 |
| MPN163 | hypothetical protein | NP_109851.1 | BBF10K_002582 |
| rplW | 50S ribosomal protein L23 | NP_109855.1 | BBF10K_002586 |
| rpsC | 30S ribosomal protein S3 | NP_109859.1 | BBF10K_002590 |
| rplP | 50S ribosomal protein L16 | NP_109860.1 | BBF10K_002591 |
| rpmC | 50S ribosomal protein L29 | NP_109861.1 | BBF10K_002592 |
| rplN | 50S ribosomal protein L14 | NP_109863.1 | BBF10K_002594 |
| rplO | 50S ribosomal protein L15 | NP_109871.1 | BBF10K_002603 |
| secY | preprotein translocase subunit SecY | NP_109872.1 | BBF10K_002604 |
| adk | adenylate kinase | NP_109873.1 | BBF10K_002605 |
| rpoA | DNA-directed RNA polymerase subunit alpha | NP_109879.1 | BBF10K_002611 |
| rpsB | 30S ribosomal protein S2 | NP_109896.1 | BBF10K_002630 |
| MPN212 | hypothetical protein | NP_109900.1 | BBF10K_002634 |
| MPN214 | hypothetical protein | NP_109902.1 | BBF10K_002636 |
| oppB | oligopeptide ABC transporter permease | NP_109903.1 | BBF10K_002637 |
| oppD | oligopeptide ABC transporter ATP-bindingprotein | NP_109905.1 | BBF10K_002639 |
| rplK | 50S ribosomal protein L11 | NP_109907.1 | BBF10K_002642 |
| rplA | 50S ribosomal protein L1 | NP_109908.1 | BBF10K_002643 |
| lgt | prolipoprotein diacylglyceryl transferase | NP_109912.1 | BBF10K_002647 |
| ssb | single-stranded DNA binding protein | NP_109917.1 | BBF10K_002653 |
| rplI | 50S ribosomal protein L9 | NP_109919.1 | BBF10K_002655 |
| gatB | aspartyl/glutamyl-tRNA amidotransferase subunitB | NP_109926.1 | BBF10K_002662 |
| MPN239 | hypothetical protein | NP_109927.1 | BBF10K_002663 |
| trxB | thioredoxin reductase | NP_109928.1 | BBF10K_002664 |
| gmk | guanylate kinase | NP_109934.1 | BBF10K_002670 |
| yjeQ | ribosome biogenesis GTPase RsgA | NP_109937.1 | BBF10K_002673 |
| galE | UDP-glucose 4-epimerase | NP_109945.1 | BBF10K_002683 |
| MPN259 | ABC transporter permease | NP_109947.1 | BBF10K_002685 |
| rbsC | ABC transporter permease | NP_109948.1 | BBF10K_002686 |
| MPN264 | hypothetical protein | NP_109952.1 | BBF10K_002690 |
| trpS | tryptophan--tRNA ligase | NP_109953.1 | BBF10K_002691 |
| MPN268 | PTS system transporter subunit IIBC | NP_109956.1 | BBF10K_002694 |
| MPN271 | lipoprotein | NP_109959.1 | BBF10K_002698 |
| hit1 | hydrolase | NP_109961.1 | BBF10K_002701 |
| MPN276 | hypothetical protein | NP_109964.1 | BBF10K_002704 |
| prrB | type I restriction-modification systemspecificity protein | NP_109973.2 | BBF10K_002712 |
| MPN290 | hypothetical protein | NP_109978.1 | BBF10K_002718 |
| yceC | RNA pseudouridine synthase | NP_109980.1 | BBF10K_002720 |
| MPN295 | hypothetical protein | NP_109983.1 | BBF10K_002723 |
| acpS | holo-ACP synthase | NP_109986.1 | BBF10K_002726 |
| pfkA | ATP-dependent 6-phosphofructokinase | NP_109990.1 | BBF10K_002730 |
| arcC | carbamate kinase | NP_109995.1 | BBF10K_002733 |
| mraW | rRNA small subunit methyltransferase H | NP_110003.1 | BBF10K_002741 |
| nrdF | ribonucleotide-diphosphate reductase subunitbeta | NP_110010.1 | BBF10K_002749 |
| nrdI | ribonucleotide reductase stimulatory protein | NP_110011.1 | BBF10K_002750 |
| rplU | 50S ribosomal protein L21 | NP_110013.1 | BBF10K_002752 |
| rpmA | 50S ribosomal protein L27 | NP_110015.1 | BBF10K_002754 |
| MPN329 | ferric uptake regulator | NP_110017.1 | BBF10K_002756 |
| tig | trigger factor | NP_110019.1 | BBF10K_002758 |
| bcrA | ABC transporter ATP-binding protein | NP_110022.1 | BBF10K_002761 |
| MPN337 | hypothetical protein | NP_110025.1 | BBF10K_002764 |
| MPN338 | hypothetical protein | NP_110026.1 | BBF10K_002765 |
| MPN344 | hypothetical protein | NP_110032.1 | BBF10K_002771 |
| MPN348 | 5-formyltetrahydrofolate cyclo-ligase | NP_110036.1 | BBF10K_002774 |
| ygiH | glycerol-3-phosphate acyltransferase | NP_110038.1 | BBF10K_002776 |
| MPN351 | Putative tRNA methyltransferase MPN_351 | NP_110039.1 | BBF10K_002777 |
| MPN359 | hypothetical protein | NP_110047.1 | BBF10K_002786 |
| fpg | formamidopyrimidine-DNA glycosylase | NP_110068.1 | BBF10K_002805 |
| MPN387 | hypothetical protein | NP_110075.1 | BBF10K_002812 |
| lplA | lipoate--protein ligase | NP_110077.1 | BBF10K_002813 |
| pdhD | pyruvate dehydrogenase complex dihydrolipoamidedehydrogenase | NP_110078.1 | BBF10K_002814 |
| pdhB | pyruvate dehydrogenase complex component E1subunit beta | NP_110080.1 | BBF10K_002817 |
| greA | transcription elongation factor GreA | NP_110089.1 | BBF10K_002827 |
| proS | proline--tRNA ligase | NP_110090.1 | BBF10K_002828 |
| MPN403 | hypothetical protein | NP_110091.1 | BBF10K_002829 |
| MPN408 | lipoprotein | NP_110096.1 | BBF10K_002836 |
| MPN410 | hypothetical protein | NP_110098.1 | BBF10K_002838 |
| MPN414 | hypothetical protein | NP_110102.1 | BBF10K_002843 |
| P29 | ABC transporter ATP-binding protein | NP_110104.1 | BBF10K_002845 |
| MPN419 | Holliday junction resolvase | NP_110106.1 | BBF10K_002847 |
| glpQ | glycerophosphoryl diester phosphodiesterase | NP_110108.1 | BBF10K_002849 |
| yidA | phosphatase | NP_110115.1 | BBF10K_002856 |
| cbiO | cobalt ABC transporter ATP-binding protein | NP_110121.1 | BBF10K_002862 |
| come3 | hypothetical protein | NP_110139.1 | BBF10K_002877 |
| MPN462 | hypothetical protein | NP_110150.1 | BBF10K_002885 |
| MPN469 | hypothetical protein | NP_110157.1 | BBF10K_002890 |
| degV | hypothetical protein | NP_110160.1 | BBF10K_002893 |
| MPN478 | transcriptional regulator | NP_110166.1 | BBF10K_002900 |
| MPN479 | FMN-dependent NADH-azoreductase | NP_110167.1 | BBF10K_002901 |
| yibD | diacylglycerol beta-glycosyltransferase | NP_110171.1 | BBF10K_002906 |
| MPN484 | hypothetical protein | NP_110172.1 | BBF10K_002907 |
| MPN488 | hypothetical protein | NP_110176.1 | BBF10K_002910 |
| recA | recombinase A | NP_110178.1 | BBF10K_002914 |
| MPN491 | hypothetical protein | NP_110179.1 | BBF10K_002915 |
| yjfU | PTS system ascorbate-specific transportersubunit IIA | NP_110182.1 | BBF10K_002919 |
| araD | L-ribulose-5-phosphate 4-epimerase | NP_110186.1 | BBF10K_002923 |
| MPN501 | hypothetical protein | NP_110189.1 | BBF10K_002926 |
| MPN504 | hypothetical protein | NP_110192.1 | BBF10K_002928 |
| MPN512 | hypothetical protein | NP_110200.1 | BBF10K_002937 |
| MPN513 | hypothetical protein | NP_110201.1 | BBF10K_002938 |
| MPN514 | hypothetical protein | NP_110202.1 | BBF10K_002939 |
| MPN517 | hypothetical protein | NP_110205.1 | BBF10K_002942 |
| ygl3 | Putative tRNA (cytidine(34)-2'-O)-methyltransferase | NP_110209.1 | BBF10K_002946 |
| MPN524 | hypothetical protein | NP_110212.1 | BBF10K_002949 |
| MPN527 | hypothetical protein | NP_110215.1 | BBF10K_002952 |
| ppa | inorganic pyrophosphatase | NP_110216.1 | BBF10K_002953 |
| MPN530 | hypothetical protein | NP_110219.1 | BBF10K_002956 |
| ackA | acetate kinase | NP_110222.1 | BBF10K_002959 |
| MPN534 | hypothetical protein | NP_110223.1 | BBF10K_002960 |
| fmt | methionyl-tRNA formyltransferase | NP_110232.1 | BBF10K_002971 |
| plsX | phosphate acyltransferase | NP_110235.1 | BBF10K_002974 |
| thrSv | threonine--tRNA ligase | NP_110242.1 | BBF10K_002981 |
| MPN554 | singe-stranded DNA-binding protein | NP_110243.1 | BBF10K_002982 |
| argS | arginine--tRNA ligase | NP_110245.1 | BBF10K_002984 |
| gidB | rRNA small subunit methyltransferase G | NP_110247.1 | BBF10K_002986 |
| MPN559 | hypothetical protein | NP_110248.1 | BBF10K_002987 |
| udk | uridine kinase | NP_110250.1 | BBF10K_002989 |
| outB | NH(3)-dependent NAD(+) synthetase | NP_110251.1 | BBF10K_002990 |
| MPN565 | hypothetical protein | NP_110254.2 | BBF10K_002993 |
| glpQ | glycerophosphoryl diester phosphodiesterase | NP_110255.1 | BBF10K_002994 |
| MPN572 | cytosol aminopeptidase | NP_110261.1 | BBF10K_003000 |
| MPN578 | hypothetical protein | NP_110267.1 | BBF10K_003006 |
| MPN579 | hypothetical protein | NP_110268.1 | BBF10K_003007 |
| MPN580 | hypothetical protein | NP_110269.1 | BBF10K_003008 |
| MPN581 | hypothetical protein | NP_110270.1 | BBF10K_003009 |
| MPN584 | hypothetical protein | NP_110273.1 | BBF10K_003013 |
| MPN586 | hypothetical protein | NP_110275.1 | BBF10K_003015 |
| MPN587 | lipoprotein | NP_110276.1 | BBF10K_003016 |
| MPN589 | hypothetical protein | NP_110278.1 | BBF10K_003018 |
| MPN591 | hypothetical protein | NP_110280.1 | BBF10K_003020 |
| MPN593 | hypothetical protein | NP_110282.1 | BBF10K_003023 |
| atpC | ATP synthase subunit epsilon | NP_110286.1 | BBF10K_003027 |
| atpD | ATP synthase subunit beta | NP_110287.1 | BBF10K_003028 |
| atpH | ATP synthase subunit delta | NP_110290.1 | BBF10K_003031 |
| MPN605 | hypothetical protein | NP_110294.1 | BBF10K_003035 |
| eno | enolase | NP_110295.1 | BBF10K_003036 |
| pstB | phosphate ABC transporter ATP-binding protein | NP_110298.1 | BBF10K_003039 |
| MPN613 | hypothetical protein | NP_110302.1 | BBF10K_003043 |
| MPN614 | hypothetical protein | NP_110303.1 | BBF10K_003044 |
| rpsI | 30S ribosomal protein S9 | NP_110305.1 | BBF10K_003046 |
| rplM | 50S ribosomal protein L13 | NP_110306.1 | BBF10K_003047 |
| MPN626 | hypothetical protein | NP_110315.1 | BBF10K_003056 |
| ptsI | phosphoenolpyruvate-protein phosphotransferase | NP_110316.1 | BBF10K_003057 |
| tpiA | triosephosphate isomerase | NP_110318.1 | BBF10K_003059 |
| MPN638 | type I restriction-modification systemspecificity protein | NP_110327.1 | BBF10K_003066 |
| MPN641 | lipoprotein | NP_110330.1 | BBF10K_003069 |
| MPN642 | lipoprotein | NP_110331.1 | BBF10K_003070 |
| MPN644 | lipoprotein | NP_110333.1 | BBF10K_003072 |
| MPN648 | hypothetical protein | NP_110337.1 | BBF10K_003076 |
| mtlA | PTS system mannitol-specific transporter subunitIIBC | NP_110340.1 | BBF10K_003079 |
| MPN657 | hypothetical protein | NP_110346.1 | BBF10K_003086 |
| trmD | NP_110348.1 | BBF10K_003088 | |
| pilB | methionine sulfoxide reductase B | NP_110351.1 | BBF10K_003091 |
| degV | hypothetical protein | NP_110353.1 | BBF10K_003093 |
| MPN666 | hypothetical protein | NP_110355.1 | BBF10K_003095 |
| MPN673 | hypothetical protein | NP_110362.1 | BBF10K_003103 |
| cysA | ABC transporter ATP-binding protein | NP_110374.1 | BBF10K_003114 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Amjed Alsultan | amjed.talib {at} qu {dot} edu {dot} iq | Iraq |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Ery Fukushima | ery.fukushima {at} ikiam {dot} edu {dot} ec | Ecuador |
| Rodolfo Marcolino | rodolfo.marcolino {at} me {dot} com | Argentina |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
Download all of this information as a CSV from our GitHub.