Mycoplasma genitalium genes (E. coli codon optimized)

Mycoplasma genitalium genes (E. coli codon optimized)

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Mycoplasma genitalium has the smallest genome of any known free-living bacteria, but grows extremely slowly (1 month per reculture) and is Biosafety Level 2. This makes studying its genes difficult for most labs, even though it is one of the best examples of minimal genomes in nature. In order to solve this, FreeGenes has synthesized 139 of 514 Mycoplasma genitalium G37 genes, codon optimized for Escherichia coli. This will allow in-vitro or in-vivo studies of Mycoplasma genitalium’s genes in the most widely available and used host organism. Hopefully, by supporting Mycoplasma research, FreeGenes can encourage synthetic biology laboratories to conduct synthetic genome research.

What is it
This is a collection of as many protein coding sequences from Mycoplasma genitalium G37 as FreeGenes could clone in a single round of cloning. They are all standardized for MoClo assembly and codon optimized for Escherichia coli.

What can it be used for
There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Mycoplasma genome.

This product is made available under the unilateral OpenMTA

All genes are present on the plate in E. coli glycerol stocks. Some or all of these items are for use only as permitted by a research exemption.


Where I can find more information

139 unique parts

Instructions for Use:

Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.

 Plate Layout

PlateSet: Mgenitalium-0 Mgenitalium-1


Gene Name NCBI ID
MG_RS00045 deoxyribonuclease WP_009885976.1
MG_RS00050 DNA primase-like protein WP_010869288.1
MG_RS00055 hypothetical protein WP_009885973.1
MG_RS00100 proline iminopeptidase WP_009885920.1
MG_RS00145 DJ-1 family protein WP_009885913.1
MG_RS00180 aspartate--tRNA ligase WP_009885700.1
MG_RS00185 nicotinate phosphoribosyltransferase WP_029329854.1
MG_RS00260 thymidine phosphorylase WP_009885713.1
MG_RS00280 preprotein translocase subunit SecE WP_010869309.1
MG_RS00285 hypothetical protein WP_009885720.1
MG_RS00295 ribonuclease M5 WP_009885722.1
MG_RS00310 glycosyl transferase family A WP_010869310.1
MG_RS00365 hypothetical protein WP_009885732.1
MG_RS00420 hypothetical protein WP_010869319.1
MG_RS00460 50S ribosomal protein L1 WP_010869323.1
MG_RS00500 30S ribosomal protein S6 WP_009885647.1
MG_RS00505 single-stranded DNA-binding protein WP_010869327.1
MG_RS00515 50S ribosomal protein L9 WP_009885649.1
MG_RS00520 replicative DNA helicase WP_010869329.1
MG_RS00575 ribonuclease R WP_009885662.1
MG_RS00580 TIGR00159 family protein WP_009885663.1
MG_RS00595 protein phosphatase WP_010869334.1
MG_RS00610 glucose-6-phosphate isomerase WP_010869336.1
MG_RS00615 ribulose-phosphate 3-epimerase WP_009885669.1
MG_RS00625 CDP-diacylglycerol--glycerol-3-phosphate3-phosphatidyltransferase WP_081440607.1
MG_RS00630 amidohydrolase WP_009885673.1
MG_RS00640 hypothetical protein WP_010869339.1
MG_RS00675 thiol reductase thioredoxin WP_010869343.1
MG_RS00680 Cof-type HAD-IIB family hydrolase WP_010869344.1
MG_RS00685 tryptophan--tRNA ligase WP_009885680.1
MG_RS00715 hypothetical protein WP_014893951.1
MG_RS00725 DUF1600 domain-containing protein WP_009885689.1
MG_RS00730 Uncharacterized protein MG134 WP_010869347.1
MG_RS00760 hypothetical protein WP_041593667.1
MG_RS00795 hypothetical protein WP_081440608.1
MG_RS00820 ribosome-binding factor A WP_009885829.1
MG_RS00845 DUF2130 domain-containing protein WP_010869355.1
MG_RS00855 hypothetical protein WP_010869357.1
MG_RS00865 50S ribosomal protein L3 WP_010869358.1
MG_RS00870 50S ribosomal protein L4 WP_009885836.1
MG_RS00875 50S ribosomal protein L23 WP_009885837.1
MG_RS00890 50S ribosomal protein L22 WP_009885840.1
MG_RS00905 50S ribosomal protein L29 WP_009885843.1
MG_RS00915 50S ribosomal protein L14 WP_009885845.1
MG_RS00940 50S ribosomal protein L6 WP_010869360.1
MG_RS00945 50S ribosomal protein L18 WP_009885853.1
MG_RS00950 30S ribosomal protein S5 WP_009885854.1
MG_RS00955 50S ribosomal protein L15 WP_010869361.1
MG_RS00965 adenylate kinase WP_009885856.1
MG_RS00980 30S ribosomal protein S13 WP_009885860.1
MG_RS00990 DNA-directed RNA polymerase subunit alpha WP_009885862.1
MG_RS00995 50S ribosomal protein L17 WP_010869362.1
MG_RS01025 adenine methylase WP_010869363.1
MG_RS01035 adhesin WP_041593682.1
MG_RS01040 hypothetical protein WP_049759242.1
MG_RS01050 hypothetical protein WP_010869364.1
MG_RS01060 ABC transporter permease WP_009886002.1
MG_RS01090 adhesin WP_041593671.1
MG_RS01140 hypothetical protein WP_010869370.1
MG_RS01175 Uncharacterized protein MG208 WP_009885745.1
MG_RS01180 RNA pseudouridine synthase WP_009885746.1
MG_RS01185 signal peptidase II WP_009885747.1
MG_RS01200 DivIVA domain-containing protein WP_009885750.1
MG_RS01210 1-acyl-sn-glycerol-3-phosphate acyltransferase WP_009885752.1
MG_RS01220 segregation/condensation protein B WP_009885754.1
MG_RS01290 hypothetical protein WP_010869379.1
MG_RS01305 division/cell wall cluster transcriptionalrepressor MraZ WP_010869380.1
MG_RS01310 ribosomal RNA small subunit methyltransferase H WP_009885760.1
MG_RS01355 class Ib ribonucleoside-diphosphate reductaseassembly flavoprotein NrdI WP_010869386.1
MG_RS01365 50S ribosomal protein L21 WP_009885769.1
MG_RS01380 endonuclease WP_009885772.1
MG_RS01385 transcriptional repressor WP_009885773.1
MG_RS01450 RNA polymerase sigma factor SigA WP_009885787.1
MG_RS01465 Uncharacterized tRNA/rRNA methyltransferase MG252 WP_010869393.1
MG_RS01470 cysteine--tRNA ligase WP_014894005.1
MG_RS01525 hypothetical protein WP_041593673.1
MG_RS01530 adhesin WP_041593674.1
MG_RS01570 dephospho-CoA kinase WP_009885895.1
MG_RS01585 hypothetical protein WP_009885898.1
MG_RS01600 hypothetical protein WP_009885901.1
MG_RS01625 pyruvate dehydrogenase E1 component subunitalpha WP_009885906.1
MG_RS01640 hypothetical protein WP_010869403.1
MG_RS01645 Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase WP_009885909.1
MG_RS01650 hypothetical protein WP_010869404.1
MG_RS01720 hypothetical protein WP_041593676.1
MG_RS01745 high affinity transport system protein p37 WP_009885873.1
MG_RS01760 Holliday junction resolvase RuvX WP_010869411.1
MG_RS01775 MFS transporter WP_014894023.1
MG_RS01805 phosphoglycerate kinase WP_010869417.1
MG_RS01860 30S ribosomal protein S4 WP_009885889.1
MG_RS01880 hypothetical protein WP_009885989.1
MG_RS01885 hypothetical protein WP_009885990.1
MG_RS01890 Cytadherence high molecular weight protein 3 WP_010869426.1
MG_RS01970 TrkA family potassium uptake protein WP_010869432.1
MG_RS01975 hypothetical protein WP_014894034.1
MG_RS02000 hypothetical protein WP_010869434.1
MG_RS02045 diacylglycerol beta-glycosyltransferase WP_010869439.1
MG_RS02055 iron-sulfur cluster scaffold-like protein WP_009885968.1
MG_RS02065 protein RecA WP_009885970.1
MG_RS02070 hypothetical protein WP_041593679.1
MG_RS02840 adhesin WP_081440609.1
MG_RS02080 adhesin WP_041593680.1
MG_RS02120 tRNA (guanine-N(7)-)-methyltransferase WP_009885805.1
MG_RS02130 hypothetical protein WP_009885806.1
MG_RS02140 hypothetical protein WP_009885807.1
MG_RS02145 hypothetical protein WP_010869445.1
MG_RS02160 inorganic pyrophosphatase WP_009885809.1
MG_RS02165 Holliday junction DNA helicase RecU WP_009885810.1
MG_RS02175 DUF1951 domain-containing protein WP_009885812.1
MG_RS02180 chaperone protein ClpB WP_010869446.1
MG_RS02190 acetate kinase WP_009885814.1
MG_RS02215 DNA polymerase IV WP_010869448.1
MG_RS02220 50S ribosomal protein L10 WP_009885818.1
MG_RS02260 DAK2 domain-containing protein WP_010869453.1
MG_RS02265 RluA family pseudouridine synthase WP_014894681.1
MG_RS02270 bifunctional oligoribonuclease/PAP phosphataseNrnA WP_010869455.1
MG_RS02310 tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG WP_010869458.1
MG_RS02320 hypothetical protein WP_010869459.1
MG_RS02350 hypothetical protein WP_009885949.1
MG_RS02370 hypothetical protein WP_009885633.1
MG_RS02390 co-chaperone GroES WP_010869464.1
MG_RS02410 ribose 5-phosphate isomerase B WP_009885626.1
MG_RS02445 ATP synthase subunit B WP_009885620.1
MG_RS02450 ATP synthase subunit C WP_009885619.1
MG_RS02470 Peptide methionine sulfoxide reductase MsrA WP_010869470.1
MG_RS02525 hypothetical protein WP_010869478.1
MG_RS02550 sigma-70 family RNA polymerase sigma factor WP_009885604.1
MG_RS02580 UMP kinase WP_009885599.1
MG_RS02600 hypothetical protein WP_009885595.1
MG_RS02610 hypothetical protein WP_009885592.1
MG_RS02630 tRNA (guanine-N(1)-)-methyltransferase WP_010869485.1
MG_RS02645 Peptide methionine sulfoxide reductase MsrB WP_009885587.1
MG_RS02675 organic hydroperoxide reductase OsmC/OhrA WP_041361276.1
MG_RS02685 hypothetical protein WP_009885577.1
MG_RS02700 hypoxanthine-guanine phosphoribosyltransferase WP_009885573.1
MG_RS02705 hypothetical protein WP_009885572.1
MG_RS02720 glutamate--tRNA ligase WP_009885570.1
MG_RS02730 protein translocase component YidC WP_009885569.1
MG_RS02765 hypothetical protein WP_009885564.1


The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact
Amjed Alsultan amjed.talib {at} qu {dot} edu {dot} iq
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org