JCVI-Syn3.0 genes (E. coli codon optimized)

Do you want to study and experiment with minimal genomes? Determine the purpose of unknown genes of essential function? Then look no further!

JCVI-Syn3.0 genes (E. coli codon optimized) is a collection of 395 essential MoClo-compatible genes from synthetic bacterium JCVI-Syn3A, recoded for expression in E. coli. With this collection, you can attempt to to recreate essential gene pathways in-vitro, purify the proteins for functional studies, or create a synthetic MoClo-compatible Mycoplasma genome.

What is JCVI-Syn3A?

For nearly 20 years, the J. Craig Venter Institute (JCVI) worked on creating a minimal synthetic bacterial genome, which they finally achieved in 2016. They named the novel synthetic organism JCVI-Syn3.0. JCVI-Syn3A, which is JCVI-Syn3.0 with 16 additional genes, is more commonly used because it has a more stable phenotype[1]. All 493 genes in JCVI-Syn3.0 are essential for growth and 149 of those genes' functions are unknown.

Collection Design

Notes

[1] John Glass, one of the leaders of the minimal genome project, noted: "Note that 2 of the genes we added to JCVI-syn3.0 to make JCVI-syn3A, which has a more stable phenotype, are pseudogenes. I see no reason for you to remake those genes since they can just be PCR amplified from genomic DNA... Note that one of those pseudogenes appears to actually be synthesized. JCVISYN3A_0602, which is a pseudogene. We know that if this gene is removed from JCVI-syn3A we get the giant cell phenotype rather than the more normal JCVI-syn3A phenotype. We know because of multiple genome sequences, that there is a frameshift in the gene relative to the same gene in other Mycoplasma mycoides strains. Still, it could be that there is some RNA editing to produce the protein. Thus, I would like you to make the protein that we think JCVISYN3A_0602 was supposed to encode."

This product is made available under the unilateral OpenMTA.

Some or all of these items are for use only as permitted by a research exemption.

Shipped as purified and dried down plasmid DNA stained with Cresol Red in five 96-well plates. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.  Plate Layouts

Prior Kit Versions

If your plates were created before July 26 2021, plate layouts can be found here.

Instructions for Use:

Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.