395 essential genes from synthetic bacterium JCVI-Syn3A, codon optimized for Escherichia coli in MoClo-compatible high-copy ampicillin resistance backbones. Shipped in Escherichia coli Top10 in 6 96 well plates.
For nearly 20 years, the J. Craig Venter Institute (JCVI) worked on creating a minimal synthetic bacterial genome, which they finally achieved in 2016. They named it JCVI-Syn3.0, though the organism JCVI-Syn3A, which is JCVI-Syn3.0 with 16 addition genes, is more commonly used because it has a more stable phenotype[1]. All 493 genes in JCVI-Syn3.0 are essential for growth and 149 of those gene's functions are unknown.
All the essential genes have been recoded for expression in Escherichia coli and have been made MoClo compatible. There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Mycoplasma genome.
Recoding was done using scripts that stochastically assigned codons to amino acids. The codon table used was the FreqB table from "Design Parameters to Control Synthetic Gene Expression in Escherichia coli", which was used because it was shown to express proteins well and was recommended to us by JCVI. All genes where inserted into the BbsI:BbsI cloning sites of pOpen_build, which is individually available through our website. 395 of the 510 JCVI-Syn3A genes are included in this distribution, 14 of which do not exist in JCVI-Syn3.0. A gene map of this distribution is attached called "freegenes-jcvi-syn3a.csv". The genes added to JCVI-Syn3.0 to make JCVI-Syn3A are attached in excel spreadsheet "Genes added to JCVI-syn3.0 to make JCVI-syn3A.xlsx", which was kindly provided by John Glass at JCVI.
NOTES:
[1] John Glass, one of the leaders of the minimal genome project, noted: "Note that 2 of the genes we added to JCVI-syn3.0 to make JCVI-syn3A, which has a more stable phenotype, are pseudogenes. I see no reason for you to remake those genes since they can just be PCR amplified from genomic DNA; although Drew may think we should make them anyway. Note that one of those pseudogenes appears to actually be synthesized. JCVISYN3A_0602, which is a pseudogene. We know that if this gene is removed from JCVI-syn3A we get the giant cell phenotype rather than the more normal JCVI-syn3A phenotype. We know because of multiple genome sequences, that there is a frameshift in the gene relative to the same gene in other Mycoplasma mycoides strains. Still, it could be that there is some RNA editing to produce the protein. Thus, I would like you to make the protein that we think JCVISYN3A_0602 was supposed to encode."
This product is made available under the unilateral OpenMTA.
395 unique parts
Plate Layout
Instructions for Use:
Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.
PlateSet: FG_JCVISYN3-distro-5 FG_JCVISYN3-distro-3 FG_JCVISYN3-distro-1 FG_JCVISYN3-distro-2 FG_JCVISYN3-distro-0 FG_JCVISYN3-distro-4
Genes
| Gene | Name | NCBI ID |
|---|---|---|
| MMSYN1_0001 | Chromosomal replication initiator protein DnaA | AMW76285.1 |
| MMSYN1_0002 | AMW76286.1 | |
| MMSYN1_0003 | Ribonuclease M5 | AMW76287.1 |
| MMSYN1_0005 | Uncharacterized protein | AMW76289.1 |
| MMSYN1_0007 | AMW76291.1 | |
| MMSYN1_0008 | ABC transporter permease | AMW76292.1 |
| MMSYN1_0009 | AMW76293.1 | |
| MMSYN1_0010 | AMW76294.1 | |
| MMSYN1_0011 | AMW76295.1 | |
| MMSYN1_0012 | AMW76296.1 | |
| MMSYN1_0029 | FMN-dependent NADH-azoreductase | AMW76300.1 |
| MMSYN1_0033 | AMW76302.1 | |
| MMSYN1_0034 | AMW76303.1 | |
| MMSYN1_0039 | AMW76304.1 | |
| MMSYN1_0040 | AMW76305.1 | |
| MMSYN1_0042 | AMW76306.1 | |
| MMSYN1_0043 | tRNA1(Val) (Adenine(37)-N6)-methyltransferase | AMW76307.1 |
| MMSYN1_0044 | DNA polymerase III delta subunit | AMW76308.1 |
| MMSYN1_0045 | AMW76309.1 | |
| MMSYN1_0046 | Recombination protein RecR | AMW76310.1 |
| MMSYN1_0047 | DNA polymerase III subunit gamma/tau | AMW76311.1 |
| MMSYN1_0054 | Peroxiredoxin | AMW76312.1 |
| MMSYN1_0060 | AMW76313.1 | |
| MMSYN1_0061 | Serine--tRNA ligase | AMW76314.1 |
| MMSYN1_0063 | tRNA-dihydrouridine synthase | AMW76315.1 |
| MMSYN1_0065 | Thioredoxin | AMW76317.1 |
| MMSYN1_0066 | AMW76318.1 | |
| MMSYN1_0077 | Cof-type HAD-IIB family hydrolase | AMW76320.1 |
| MMSYN1_0079 | tRNA N6-adenosine threonylcarbamoyltransferase | AMW76321.1 |
| MMSYN1_0094 | Putative membrane protein | AMW76325.1 |
| MMSYN1_0097 | ~5'-3' exonuclease | AMW76327.1 |
| MMSYN1_0106 | AMW76329.1 | |
| MMSYN1_0107 | Transcription antitermination protein NusB | AMW76330.1 |
| MMSYN1_0108 | AMW76331.1 | |
| MMSYN1_0109 | Probable endonuclease 4 | AMW76332.1 |
| MMSYN1_0113 | AMW76333.1 | |
| MMSYN1_0115 | AMW76335.1 | |
| MMSYN1_0116 | Uncharacterized protein | AMW76336.1 |
| MMSYN1_0117 | Glycerol-3-phosphate acyltransferase | AMW76337.1 |
| MMSYN1_0126 | Glutamate--tRNA ligase | AMW76338.1 |
| MMSYN1_0127 | HD domain-containing protein | AMW76339.1 |
| MMSYN1_0128 | DNA-directed RNA polymerase subunit delta | AMW76340.1 |
| MMSYN1_0129 | AMW76341.1 | |
| MMSYN1_0131 | Fructose-1 | AMW76342.1 |
| MMSYN1_0132 | AMW76343.1 | |
| MMSYN1_0133 | Peptidase_S8 domain-containing protein | AMW76344.1 |
| MMSYN1_0138 | Uncharacterized protein | AMW76346.1 |
| MMSYN1_0139 | Bifunctional oligoribonuclease/PAP phosphatase NrnA | AMW76347.1 |
| MMSYN1_0141 | Peptide chain release factor 1 | AMW76349.1 |
| MMSYN1_0142 | Release factor glutamine methyltransferase | AMW76350.1 |
| MMSYN1_0143 | AMW76351.1 | |
| MMSYN1_0144 | YrdC-like domain-containing protein | AMW76352.1 |
| MMSYN1_0145 | AMW76353.1 | |
| MMSYN1_0146 | AMW76354.1 | |
| MMSYN1_0147 | AMW76355.1 | |
| MMSYN1_0148 | 30S ribosomal protein S12 | AMW76356.1 |
| MMSYN1_0150 | Elongation factor G | AMW76358.1 |
| MMSYN1_0151 | Elongation factor Tu | AMW76359.1 |
| MMSYN1_0154 | Leucyl aminopeptidase | AMW76531.1 |
| MMSYN1_0163 | AMW76360.1 | |
| MMSYN1_0164 | AMW76361.1 | |
| MMSYN1_0165 | ABC transporter permease | AMW76362.1 |
| MMSYN1_0166 | ABC transporter permease | AMW76363.1 |
| MMSYN1_0167 | Peptide ABC transporter ATP-binding protein | AMW76364.1 |
| MMSYN1_0168 | AMW76365.1 | |
| MMSYN1_0196 | AMW76368.1 | |
| MMSYN1_0197 | Spermidine/putrescine import ATP-binding protein PotA | AMW76369.1 |
| MMSYN1_0198 | 50S ribosomal protein L20 | AMW76370.1 |
| MMSYN1_0200 | Translation initiation factor IF-3 | AMW76372.1 |
| MMSYN1_0202 | ~16S rRNA (Guanine(966)-N(2))-methyltransferase RsmD | AMW76374.1 |
| MMSYN1_0203 | Guanylate kinase | AMW76375.1 |
| MMSYN1_0213 | ||
| MMSYN1_0214 | AMW76377.1 | |
| MMSYN1_0215 | Putative pre-16S rRNA nuclease | AMW76378.1 |
| MMSYN1_0216 | AMW76379.1 | |
| MMSYN1_0218 | AMW76380.1 | |
| MMSYN1_0220 | ATP-dependent 6-phosphofructokinase | AMW76381.1 |
| MMSYN1_0221 | Pyruvate kinase | AMW76382.1 |
| MMSYN1_0227 | AMW76384.1 | |
| MMSYN1_0228 | AMW76385.1 | |
| MMSYN1_0229 | Phosphate acetyltransferase | AMW76386.1 |
| MMSYN1_0230 | AMW76387.1 | |
| MMSYN1_0233 | Phosphoenolpyruvate-protein phosphotransferase | AMW76388.1 |
| MMSYN1_0234 | PTS glucose transporter subunit IIA | AMW76389.1 |
| MMSYN1_0238 | 30S ribosomal protein S4 | AMW76391.1 |
| MMSYN1_0239 | Uncharacterized protein | AMW76392.1 |
| MMSYN1_0240 | AMW76393.1 | |
| MMSYN1_0247 | Probable GTP-binding protein EngB | AMW76394.1 |
| MMSYN1_0248 | Uncharacterized protein | AMW76395.1 |
| MMSYN1_0253 | Transcription elongation factor GreA | AMW76398.1 |
| MMSYN1_0254 | AMW76399.1 | |
| MMSYN1_0257 | AMW76400.1 | |
| MMSYN1_0259 | AMW76401.1 | |
| MMSYN1_0260 | Valine--tRNA ligase | AMW76402.1 |
| MMSYN1_0262 | Ribulose-phosphate 3-epimerase | AMW76403.1 |
| MMSYN1_0263 | Small ribosomal subunit biogenesis GTPase RsgA | AMW76404.1 |
| MMSYN1_0264 | Serine/threonine protein kinase | AMW76405.1 |
| MMSYN1_0270 | tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE | AMW76406.1 |
| MMSYN1_0271 | tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB | AMW76407.1 |
| MMSYN1_0281 | Uncharacterized protein | AMW76408.1 |
| MMSYN1_0282 | AMW76409.1 | |
| MMSYN1_0283 | Ribonuclease H | AMW76410.1 |
| MMSYN1_0285 | Elongation factor 4 | AMW76411.1 |
| MMSYN1_0286 | Uncharacterized protein | AMW76412.1 |
| MMSYN1_0287 | AMW76413.1 | |
| MMSYN1_0288 | Histidine--tRNA ligase | AMW76414.1 |
| MMSYN1_0289 | Ribosome-binding factor A | AMW76415.1 |
| MMSYN1_0290 | AMW76416.1 | |
| MMSYN1_0291 | FAD synthetase | AMW76417.1 |
| MMSYN1_0296 | Uncharacterized protein | AMW76419.1 |
| MMSYN1_0298 | ~50S ribosomal protein L7ae | AMW76421.1 |
| MMSYN1_0300 | AMW76423.1 | |
| MMSYN1_0301 | AMW76424.1 | |
| MMSYN1_0302 | AMW76425.1 | |
| MMSYN1_0304 | Phosphatidate cytidylyltransferase | AMW76427.1 |
| MMSYN1_0305 | Aminopeptidase P family protein | AMW76428.1 |
| MMSYN1_0308 | AMW76429.1 | |
| MMSYN1_0314 | Folate family ECF transporter S component | AMW76430.1 |
| MMSYN1_0316 | AMW76432.1 | |
| MMSYN1_0325 | AMW76434.1 | |
| MMSYN1_0326 | Uncharacterized protein | AMW76435.1 |
| MMSYN1_0327 | AMW76436.1 | |
| MMSYN1_0328 | AMW76437.1 | |
| MMSYN1_0329 | rRNA pseudouridine synthase | AMW76438.1 |
| MMSYN1_0330 | Deoxynucleoside kinase | AMW76439.1 |
| MMSYN1_0332 | AMW76440.1 | |
| MMSYN1_0338 | Lipoprotein | AMW76441.1 |
| MMSYN1_0344 | Inorganic pyrophosphatase | AMW76442.1 |
| MMSYN1_0345 | ECF transporter S component | AMW76443.1 |
| MMSYN1_0346 | Uncharacterized protein | AMW76444.1 |
| MMSYN1_0352 | DnaD family protein | AMW76448.1 |
| MMSYN1_0353 | DivIVA domain-containing protein | AMW76449.1 |
| MMSYN1_0359 | AMW76450.1 | |
| MMSYN1_0360 | AMW76451.1 | |
| MMSYN1_0361 | Ribosomal RNA large subunit methyltransferase H | AMW76452.1 |
| MMSYN1_0363 | Ribosome maturation factor RimM | AMW76454.1 |
| MMSYN1_0364 | AMW76455.1 | |
| MMSYN1_0365 | 50S ribosomal protein L19 | AMW76456.1 |
| MMSYN1_0366 | Ribosome biogenesis GTPase A | AMW76457.1 |
| MMSYN1_0371 | AMW76458.1 | |
| MMSYN1_0373 | AMW76460.1 | |
| MMSYN1_0375 | AMW76461.1 | |
| MMSYN1_0376 | AMW76462.1 | |
| MMSYN1_0378 | AMW76464.1 | |
| MMSYN1_0380 | AMW76466.1 | |
| MMSYN1_0381 | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | AMW76467.1 |
| MMSYN1_0387 | AMW76469.1 | |
| MMSYN1_0388 | Uncharacterized protein | AMW76470.1 |
| MMSYN1_0389 | Uncharacterized protein | AMW76471.1 |
| MMSYN1_0390 | Methionyl-tRNA formyltransferase | AMW76472.1 |
| MMSYN1_0391 | Elongation factor P | AMW76473.1 |
| MMSYN1_0392 | Uncharacterized protein | AMW76474.1 |
| MMSYN1_0394 | AMW76475.1 | |
| MMSYN1_0398 | MOLPALP family lipoprotein | |
| MMSYN1_0401 | AMW76479.1 | |
| MMSYN1_0402 | Endoribonuclease YbeY | AMW76480.1 |
| MMSYN1_0403 | AMW76481.1 | |
| MMSYN1_0404 | DNA repair protein RecO | AMW76482.1 |
| MMSYN1_0405 | AMW76483.1 | |
| MMSYN1_0406 | AMW76484.1 | |
| MMSYN1_0407 | RNA polymerase sigma factor | AMW76485.1 |
| MMSYN1_0408 | AMW76486.1 | |
| MMSYN1_0409 | AMW76487.1 | |
| MMSYN1_0410 | ATP-dependent helicase | AMW76488.1 |
| MMSYN1_0411 | AMW76489.1 | |
| MMSYN1_0412 | Putative membrane protein | AMW76490.1 |
| MMSYN1_0413 | Adenine phosphoribosyltransferase | AMW76491.1 |
| MMSYN1_0416 | Uncharacterized protein | AMW76494.1 |
| MMSYN1_0418 | Ribonuclease 3 | AMW76495.1 |
| MMSYN1_0420 | AMW76497.1 | |
| MMSYN1_0421 | AMW76498.1 | |
| MMSYN1_0424 | Uncharacterized protein | AMW76500.1 |
| MMSYN1_0425 | AMW76501.1 | |
| MMSYN1_0426 | Phosphate ABC transporter permease PtsA | AMW76502.1 |
| MMSYN1_0427 | Phosphate import ATP-binding protein PstB | AMW76503.1 |
| MMSYN1_0428 | AMW76504.1 | |
| MMSYN1_0430 | AMW76506.1 | |
| MMSYN1_0431 | TIGR00282 family metallophosphoesterase | AMW76507.1 |
| MMSYN1_0434 | Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO | AMW76510.1 |
| MMSYN1_0435 | Phosphomannose isomerase type I | AMW76511.1 |
| MMSYN1_0437 | HD domain-containing protein | AMW76512.1 |
| MMSYN1_0438 | AMW76513.1 | |
| MMSYN1_0439 | AMW76514.1 | |
| MMSYN1_0441 | AMW76516.1 | |
| MMSYN1_0442 | Iron-sulfur cluster assembly scaffold protein | AMW76517.1 |
| MMSYN1_0443 | 5-formyltetrahydrofolate cyclo-ligase | AMW76518.1 |
| MMSYN1_0444 | AMW76519.1 | |
| MMSYN1_0445 | AMW76520.1 | |
| MMSYN1_0447 | dUTPase superfamily protein | AMW76521.1 |
| MMSYN1_0448 | AMW76522.1 | |
| MMSYN1_0451 | NADP-dependent glyceraldehyde-3-phosphate dehydrogenase | AMW76523.1 |
| MMSYN1_0452 | AMW76524.1 | |
| MMSYN1_0475 | AMW76526.1 | |
| MMSYN1_0478 | Uncharacterized protein | AMW76527.1 |
| MMSYN1_0479 | Uncharacterized protein | AMW76528.1 |
| MMSYN1_0481 | Lipoprotein (VlcE) | AMW76529.1 |
| MMSYN1_0493 | Xaa-His dipeptidase | AMW76532.1 |
| MMSYN1_0494 | Putative N-acetylmannosamine-6-phosphate 2-epimerase | AMW76533.1 |
| MMSYN1_0495 | ROK family protein | AMW76534.1 |
| MMSYN1_0501 | 50S ribosomal protein L21 | AMW76537.1 |
| MMSYN1_0503 | Uncharacterized protein | AMW76538.1 |
| MMSYN1_0504 | Ribosomal RNA small subunit methyltransferase I | AMW76539.1 |
| MMSYN1_0511 | Uncharacterized protein | AMW76541.1 |
| MMSYN1_0512 | AMW76542.1 | |
| MMSYN1_0513 | Holo-[acyl-carrier-protein] synthase | AMW76543.1 |
| MMSYN1_0515 | dCMP deaminase | AMW76544.1 |
| MMSYN1_0517 | AMW76546.1 | |
| MMSYN1_0518 | AMW76547.1 | |
| MMSYN1_0523 | Uncharacterized protein | AMW76549.1 |
| MMSYN1_0524 | Ribosomal RNA small subunit methyltransferase H | AMW76550.1 |
| MMSYN1_0525 | Transcriptional regulator MraZ | AMW76551.1 |
| MMSYN1_0528 | Phenylalanine--tRNA ligase beta subunit | AMW76553.1 |
| MMSYN1_0529 | Phenylalanine--tRNA ligase alpha subunit | AMW76554.1 |
| MMSYN1_0530 | Uncharacterized protein | AMW76555.1 |
| MMSYN1_0531 | ABC transporter permease | |
| MMSYN1_0535 | AMW76557.1 | |
| MMSYN1_0536 | Ribosome-recycling factor | AMW76558.1 |
| MMSYN1_0539 | AMW76560.1 | |
| MMSYN1_0540 | 30S ribosomal protein S2 | AMW76561.1 |
| MMSYN1_0541 | AMW76562.1 | |
| MMSYN1_0542 | AMW76563.1 | |
| MMSYN1_0543 | Protein GrpE | AMW76564.1 |
| MMSYN1_0544 | Heat-inducible transcription repressor HrcA | AMW76565.1 |
| MMSYN1_0545 | AMW76566.1 | |
| MMSYN1_0601 | AMW76569.1 | |
| MMSYN1_0606 | AMW76570.1 | |
| MMSYN1_0607 | Glyceraldehyde-3-phosphate dehydrogenase | AMW76571.1 |
| MMSYN1_0608 | AMW76572.1 | |
| MMSYN1_0609 | Chromosome replication initiation/membrane attachment protein | AMW76573.1 |
| MMSYN1_0611 | AMW76574.1 | |
| MMSYN1_0612 | AMW76575.1 | |
| MMSYN1_0613 | Tyrosine--tRNA ligase | AMW76576.1 |
| MMSYN1_0614 | AMW76577.1 | |
| MMSYN1_0615 | Phenylalanine--tRNA ligase beta subunit | AMW76578.1 |
| MMSYN1_0616 | AMW76579.1 | |
| MMSYN1_0617 | DegV family protein | AMW76580.1 |
| MMSYN1_0620 | Transcriptional repressor | AMW76581.1 |
| MMSYN1_0632 | AMW76583.1 | |
| MMSYN1_0634 | Leucine--tRNA ligase | AMW76584.1 |
| MMSYN1_0636 | AMW76585.1 | |
| MMSYN1_0637 | 30S ribosomal protein S9 | AMW76586.1 |
| MMSYN1_0638 | AMW76587.1 | |
| MMSYN1_0639 | AMW76588.1 | |
| MMSYN1_0640 | tRNA pseudouridine synthase A | AMW76589.1 |
| MMSYN1_0641 | Energy-coupling factor transporter transmembrane protein EcfT | AMW76590.1 |
| MMSYN1_0642 | Energy-coupling factor transporter ATP-binding protein EcfA | AMW76591.1 |
| MMSYN1_0643 | AMW76592.1 | |
| MMSYN1_0644 | AMW76593.1 | |
| MMSYN1_0645 | DNA-directed RNA polymerase subunit alpha | AMW76594.1 |
| MMSYN1_0646 | 30S ribosomal protein S11 | AMW76595.1 |
| MMSYN1_0647 | 30S ribosomal protein S13 | AMW76596.1 |
| MMSYN1_0650 | AMW76599.1 | |
| MMSYN1_0651 | Adenylate kinase | |
| MMSYN1_0652 | Protein translocase subunit SecY | AMW76601.1 |
| MMSYN1_0653 | 50S ribosomal protein L15 | AMW76602.1 |
| MMSYN1_0654 | 30S ribosomal protein S5 | AMW76603.1 |
| MMSYN1_0655 | AMW76604.1 | |
| MMSYN1_0656 | 50S ribosomal protein L6 | AMW76605.1 |
| MMSYN1_0657 | 30S ribosomal protein S8 | AMW76606.1 |
| MMSYN1_0659 | 50S ribosomal protein L5 | AMW76608.1 |
| MMSYN1_0660 | 50S ribosomal protein L24 | AMW76609.1 |
| MMSYN1_0661 | 50S ribosomal protein L14 | AMW76610.1 |
| MMSYN1_0663 | 50S ribosomal protein L29 | AMW76612.1 |
| MMSYN1_0664 | 50S ribosomal protein L16 | AMW76613.1 |
| MMSYN1_0665 | 30S ribosomal protein S3 | AMW76614.1 |
| MMSYN1_0666 | 50S ribosomal protein L22 | AMW76615.1 |
| MMSYN1_0668 | 50S ribosomal protein L2 | AMW76617.1 |
| MMSYN1_0670 | 50S ribosomal protein L4 | AMW76619.1 |
| MMSYN1_0671 | 50S ribosomal protein L3 | AMW76620.1 |
| MMSYN1_0672 | 30S ribosomal protein S10 | AMW76621.1 |
| MMSYN1_0684 | Bifunctional protein FolD | AMW76622.1 |
| MMSYN1_0685 | Sodium transporter | AMW76623.1 |
| MMSYN1_0686 | TrkA family potassium uptake protein | AMW76624.1 |
| MMSYN1_0687 | AMW76625.1 | |
| MMSYN1_0688 | AMW76626.1 | |
| MMSYN1_0690 | DNA ligase | AMW76628.1 |
| MMSYN1_0691 | AMW76629.1 | |
| MMSYN1_0692 | RluA family pseudouridine synthase | AMW76630.1 |
| MMSYN1_0693 | AMW76631.1 | |
| MMSYN1_0695 | AMW76633.1 | |
| MMSYN1_0696 | RDD family protein | AMW76634.1 |
| MMSYN1_0697 | Glycosyltransferase family 2 protein | AMW76635.1 |
| MMSYN1_0706 | AMW76636.1 | |
| MMSYN1_0707 | Alkylphosphonate ABC transporter ATP-binding protein | AMW76637.1 |
| MMSYN1_0708 | AMW76638.1 | |
| MMSYN1_0710 | Cof-type HAD-IIB family hydrolase | AMW76639.1 |
| MMSYN1_0727 | AMW76641.1 | |
| MMSYN1_0729 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase | AMW76643.1 |
| MMSYN1_0730 | Uncharacterized protein | AMW76644.1 |
| MMSYN1_0732 | Deoxyribose-phosphate aldolase | AMW76645.1 |
| MMSYN1_0733 | Phospho-sugar mutase | AMW76646.1 |
| MMSYN1_0747 | Purine-nucleoside phosphorylase | AMW76647.1 |
| MMSYN1_0772 | Protein NrdI | AMW76649.1 |
| MMSYN1_0773 | Class 1b ribonucleoside-diphosphate reductase subunit beta | AMW76650.1 |
| MMSYN1_0775 | Ribonuclease R | AMW76652.1 |
| MMSYN1_0776 | SsrA-binding protein | AMW76653.1 |
| MMSYN1_0777 | Uncharacterized protein | AMW76654.1 |
| MMSYN1_0779 | AMW76656.1 | |
| MMSYN1_0787 | Magnesium-translocating P-type ATPase | AMW76657.1 |
| MMSYN1_0789 | ATP-synt_DE_N domain-containing protein | AMW76658.1 |
| MMSYN1_0790 | AMW76659.1 | |
| MMSYN1_0791 | ATP synthase gamma chain | AMW76660.1 |
| MMSYN1_0792 | ATP synthase subunit alpha | AMW76661.1 |
| MMSYN1_0793 | ATP synthase subunit delta | AMW76662.1 |
| MMSYN1_0794 | ATP synthase subunit b | AMW76663.1 |
| MMSYN1_0795 | ATP synthase subunit c | AMW76664.1 |
| MMSYN1_0796 | F0F1 ATP synthase subunit A | AMW76665.1 |
| MMSYN1_0798 | Uracil phosphoribosyltransferase | AMW76667.1 |
| MMSYN1_0799 | Serine hydroxymethyltransferase | AMW76668.1 |
| MMSYN1_0800 | Ribose 5-phosphate isomerase B | AMW76669.1 |
| MMSYN1_0803 | AMW76670.1 | |
| MMSYN1_0805 | Uncharacterized protein | AMW76672.1 |
| MMSYN1_0807 | 50S ribosomal protein L10 | AMW76674.1 |
| MMSYN1_0809 | AMW76675.1 | |
| MMSYN1_0810 | 50S ribosomal protein L11 | AMW76676.1 |
| MMSYN1_0813 | AMW76677.1 | |
| MMSYN1_0814 | AMW76678.1 | |
| MMSYN1_0817 | Probable cell division protein WhiA | AMW76679.1 |
| MMSYN1_0818 | Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase | AMW76680.1 |
| MMSYN1_0819 | Thioredoxin reductase | AMW76681.1 |
| MMSYN1_0821 | HPr kinase/phosphorylase | AMW76683.1 |
| MMSYN1_0822 | Folate family ECF transporter S component | AMW76684.1 |
| MMSYN1_0823 | Folate synthetase-polyglutamyl folate synthetase | AMW76685.1 |
| MMSYN1_0824 | AMW76686.1 | |
| MMSYN1_0825 | AMW76687.1 | |
| MMSYN1_0826 | DNA polymerase III subunit delta | AMW76688.1 |
| MMSYN1_0827 | AMW76689.1 | |
| MMSYN1_0831 | Ribose-phosphate pyrophosphokinase | AMW76691.1 |
| MMSYN1_0832 | Peptidyl-tRNA hydrolase | AMW76692.1 |
| MMSYN1_0833 | 50S ribosomal protein L9 | AMW76693.1 |
| MMSYN1_0834 | Replicative DNA helicase | AMW76694.1 |
| MMSYN1_0835 | Uncharacterized protein | AMW76695.1 |
| MMSYN1_0836 | AMW76696.1 | |
| MMSYN1_0837 | AMW76697.1 | |
| MMSYN1_0838 | ~23S rRNA (Guanosine(2251)-2'-O)-methyltransferase RlmB | AMW76698.1 |
| MMSYN1_0839 | Preprotein translocase subunit SecE | AMW76699.1 |
| MMSYN1_0840 | Transcription termination/antitermination protein NusG | AMW76700.1 |
| MMSYN1_0853 | Uncharacterized protein | AMW76703.1 |
| MMSYN1_0870 | YfcC family protein | AMW76705.1 |
| MMSYN1_0872 | Ribosome-binding ATPase YchF | AMW76706.1 |
| MMSYN1_0874 | AMW76708.1 | |
| MMSYN1_0875 | AMW76709.1 | |
| MMSYN1_0876 | AMW76710.1 | |
| MMSYN1_0877 | AMW76711.1 | |
| MMSYN1_0879 | AMW76713.1 | |
| MMSYN1_0881 | AMW76714.1 | |
| MMSYN1_0885 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG | AMW76715.1 |
| MMSYN1_0886 | Dicarboxylate/amino acid:cation symporter | AMW76716.1 |
| MMSYN1_0887 | CoA-disulfide reductase | AMW76717.1 |
| MMSYN1_0906 | Choline/ethanolamine kinase | AMW76718.1 |
| MMSYN1_0907 | Cof-type HAD-IIB family hydrolase | AMW76719.1 |
| MMSYN1_0908 | Membrane protein insertase YidC | AMW76720.1 |
| MMSYN1_0648 | 50S ribosomal protein L36 | AMW76597.1 |
| MMSYN1_0689 | Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit C | AMW76627.1 |
| MMSYN1_0910 | 50S ribosomal protein L34 | AMW76722.1 |
| MMSYN1_0235 | AMW76390.1 | |
| MMSYN1_0669 | 50S ribosomal protein L23 | AMW76618.1 |
| MMSYN1_0315 | Uncharacterized protein | AMW76431.1 |
| MMSYN1_0774 | Protein-export membrane protein SecG | AMW76651.1 |
| MMSYN1_0482 | 30S ribosomal protein S21 | AMW76530.1 |
| MMSYN1_0299 | DUF448 domain-containing protein | AMW76422.1 |
| MMSYN1_0499 | 50S ribosomal protein L27 | AMW76535.1 |
| MMSYN1_0851 | Putative lipoprotein | AMW76701.1 |
| MMSYN1_0379 | AMW76465.1 | |
| MMSYN1_0137 | AMW76345.1 | |
| MMSYN1_0362 | 30S ribosomal protein S16 | AMW76453.1 |
| MMSYN1_0350 | HU family DNA-binding protein | AMW76447.1 |
| MMSYN1_0873 | Uncharacterized protein | AMW76707.1 |
| MMSYN1_0694 | HPr family phosphocarrier protein | AMW76632.1 |
| MMSYN1_0667 | AMW76616.1 | |
| MMSYN1_0621 | AMW76582.1 | |
| MMSYN1_0294 | 30S ribosomal protein S15 | AMW76418.1 |
| MMSYN1_0080 | Uncharacterized protein | AMW76322.1 |
| MMSYN1_0649 | Translation initiation factor IF-1 | AMW76598.1 |
| MMSYN1_0830 | Uncharacterized protein | AMW76690.1 |
| MMSYN1_0662 | 30S ribosomal protein S17 | AMW76611.1 |
| MMSYN1_0852 | Uncharacterized protein | AMW76702.1 |
| MMSYN1_0778 | Uncharacterized protein | AMW76655.1 |
| MMSYN1_0797 | AMW76666.1 | |
| MMSYN1_0317 | Uncharacterized protein | AMW76433.1 |
| MMSYN1_0082 | AMW76324.1 | |
| JCVISYN3A_0520 | AB hydrolase-1 domain-containing protein | N/A |
| JCVISYN3A_0521 | Cell division protein SepF | N/A |
| JCVISYN3A_0522 | N/A | |
| JCVISYN3A_0527 | Uncharacterized protein | N/A |
| JCVISYN3A_0538 | Uncharacterized protein | N/A |
| JCVISYN3A_0548 | N/A | |
| JCVISYN3A_0549 | dITP/XTP pyrophosphatase | N/A |
| JCVISYN3A_0592 | N/A | |
| JCVISYN3A_0593 | Uncharacterized protein | N/A |
| JCVISYN3A_0604 | LemA family protein | N/A |
| JCVISYN3A_0605 | Uncharacterized protein | N/A |
| JCVISYN3A_0610 | Formamidopyrimidine-DNA glycosylase | N/A |
| JCVISYN3A_0622 | Uncharacterized protein | N/A |
| JCVISYN3A_0623 | N/A |