
What is it?
“Clonetegration” is a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.
What can it be used for?
Integrating your favorite DNA into specific loci on a bacterial chromosome.
Where can I find more information?
One-Step Cloning and Chromosomal Integration of DNA, François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin, https://pubs.acs.org/doi/full/10.1021/sb400021j?src=recsys&
The One-Step Cloning and Chromosomal Integration of DNA Toolkit is shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Cresol Red will not impact plasmid transformation.
If you've already received the plate, use Chrome or Firefox to download the plate layout. Plates are labelled "One Step Integration Collection"
Genes
Gene | Name | Freegenes ID |
---|---|---|
pE-FLP | pE-FLP | BBF10K_000016 |
pOSIP-CH (CamR, HK022) | pOSIP-CH (CamR, HK022) | BBF10K_000031 |
pOSIP-KO (KanR, 186) | pOSIP-KO (KanR, 186) | BBF10K_000492 |
pOSIP-TT (TetR, P21) | pOSIP-TT (TetR, P21) | BBF10K_000493 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.Name | Contact | Country |
---|---|---|
Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
Shivang Joshi | shivangjoshis4 {at} gmail {dot} com | United Kingdom |
Maan Neamah | maanm.neamah {at} uokufa {dot} edu {dot} iq | Iraq |
Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
Yan Zhang | yz473 {at} gatech {dot} edu | United States |
Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
Mikhail Khvochtchev | mikhail.khv {at} mahidol {dot} edu | Thailand |
kangquan YIN | yinkq {at} bjfu {dot} edu {dot} cn | China |
Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
Benjamin Chavez | chavez {at} ipk-gatersleben {dot} de | Germany |
Franklin Nobrega | f.nobrega {at} soton {dot} ac {dot} uk | United Kingdom |
Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
Ashton Cropp | tacropp {at} vcu {dot} edu | United States |
Jae-Seong Yang | jaeseong.yang {at} cragenomica {dot} es | Spain |
Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
Fernan Federici | ffederici {at} bio {dot} puc {dot} cl | Chile |
Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
Amjed Alsultan | NA | Iraq |
Vincent Paris | NA | United States |
Rodolfo Marcolino | rodolfo.marcolino {at} me {dot} com | Argentina |
Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
Julie Legault | julie {at} amino {dot} bio | Canada |
Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
robson tramontina | robson.tramontina {at} gmail {dot} com | Brazil |
Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
Phil Roche | philroche365 {at} gmail {dot} com | Canada |
Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
Jennifer Molloy | jcm80 {at} cam {dot} ac {dot} uk | United Kingdom |
Kyle Erlenbeck | krerlenbeck {at} dcsdk12 {dot} org | United States |
Download all of this information as a CSV from our GitHub.