One-Step Cloning and Chromosomal Integration of DNA

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What is it?

“Clonetegration” is a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.

What can it be used for?

Integrating your favorite DNA into specific loci on a bacterial chromosome.

Where can I find more information?

One-Step Cloning and Chromosomal Integration of DNA, François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin, https://pubs.acs.org/doi/full/10.1021/sb400021j?src=recsys&

The One-Step Cloning and Chromosomal Integration of DNA Toolkit is available in two formats: E. coli glycerol stocks and purified nucleic acids. Please specify in the order notes if you need a particular format.

If you've already received the plate, use Chrome or Firefox to download the plate layout. Plates are labelled "One Step Integration Collection"

Genes

Gene	Name	Freegenes ID
pE-FLP	pE-FLP	BBF10K_000016
pOSIP-CH (CamR, HK022)	pOSIP-CH (CamR, HK022)	BBF10K_000031
pOSIP-KO (KanR, 186)	pOSIP-KO (KanR, 186)	BBF10K_000492
pOSIP-TT (TetR, P21)	pOSIP-TT (TetR, P21)	BBF10K_000493

Download all of this information as a CSV from our GitHub.

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name	Contact	Country
Rodolfo Marcolino	rodolfo.marcolino {at} me {dot} com	Argentina
Simran Kushwaha	https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty	India
Kefentse Arnold Tumedi	tumediak {at} yahoo {dot} com	Botswana
Phil Roche	philroche365 {at} gmail {dot} com	Canada
Ahmed Atef	ahmed_atefaig2 {at} yahoo {dot} com	Saudi Arabia
Shree Ram Singh	singhlabasu {at} gmail {dot} com	United States
robson tramontina	robson.tramontina {at} gmail {dot} com	Brazil
Peer Schenk	reuben.brown {at} uqconnect {dot} edu {dot} au	Australia
Cauã Westman	caua.westmann {at} ieu {dot} uzh {dot} ch	Switzerland
Franklin Nobrega	f.nobrega {at} soton {dot} ac {dot} uk	United Kingdom
Kenneth Frimpong	ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh	Ghana
Fristot Elsa	elsa.fristot {at} cbs {dot} cnrs {dot} fr	France
Fernan Federici	ffederici {at} bio {dot} puc {dot} cl	Chile
Ben Thuronyi	bwt2 {at} williams {dot} edu	United States
Majed Alghamdi	mafa2 {at} le {dot} ac {dot} uk	United Kingdom
Erman ÖZER	aracnoid {at} gmail {dot} com	Turkey
Swaraj Kunal	swaraj.avisa {at} gmail {dot} com	India
kangquan YIN	yinkq {at} bjfu {dot} edu {dot} cn	China
Jennifer Molloy	jcm80 {at} cam {dot} ac {dot} uk	United Kingdom
Jose Munoz	Jose.Munoz {at} northumbria {dot} ac {dot} uk	United Kingdom
Maan Neamah	maanm.neamah {at} uokufa {dot} edu {dot} iq	Iraq
Han Teng Wong	wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg	Singapore
Ahmed Hegazy	a.hegazy {at} biotec {dot} rwth-aachen {dot} de	Germany
John Allan	john.allan {at} northumbria {dot} ac {dot} uk	United Kingdom
Ahmad Suparmin	ahmad.suparmin {at} ugm {dot} ac {dot} id	Indonesia
Jordan Gonzalez	jgonzalez {at} thecitizensciencelab {dot} org	United States
Juan Vicente Farizano	juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar	Argentina
Julie Legault	julie {at} amino {dot} bio	Canada
Aleksandr Shilovich	mercurialbadger {at} yandex {dot} ru	Russia
Keoni Gandall	koeng101 {at} gmail {dot} com	United States
Jae-Seong Yang	jaeseong.yang {at} cragenomica {dot} es	Spain
Xingyue jiang	karma5781 {at} 21cn {dot} com	China

Download all of this information as a CSV from our GitHub.

Back to Endy Lab at Stanford University

MTA

Unilateral OpenMTA

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