What is it?
“Clonetegration” is a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.
What can it be used for?
Integrating your favorite DNA into specific loci on a bacterial chromosome.
Where can I find more information?
One-Step Cloning and Chromosomal Integration of DNA, François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin, https://pubs.acs.org/doi/full/10.1021/sb400021j?src=recsys&
The One-Step Cloning and Chromosomal Integration of DNA Toolkit is shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
If you've already received the plate, use Chrome or Firefox to download the plate layout. Plates are labelled "One Step Integration Collection"
This kit is included in kit plate 2 of the 2022 iGEM distribution. The plate map for kit plate 2 of the iGEM 2022 distribution can be found here.
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| pE-FLP | pE-FLP | BBF10K_000016 |
| pOSIP-CH (CamR, HK022) | pOSIP-CH (CamR, HK022) | BBF10K_000031 |
| pOSIP-KO (KanR, 186) | pOSIP-KO (KanR, 186) | BBF10K_000492 |
| pOSIP-TT (TetR, P21) | pOSIP-TT (TetR, P21) | BBF10K_000493 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Amjed Alsultan | NA | Iraq |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| John Allan | john.allan {at} eng {dot} ox {dot} ac {dot} uk | United Kingdom |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Moritz Venne | moritz.venne {at} web {dot} de | Germany |
| Kyle Erlenbeck | krerlenbeck {at} dcsdk12 {dot} org | United States |
| Mikhail Khvochtchev | mikhail.khv {at} mahidol {dot} edu | Thailand |
| Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Amir Kashani | NA | Canada |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Meng Lu | menglu {at} iastate {dot} edu | United States |
| Jonathan Jonathan | Jonathancontact16 {at} gmail {dot} com | Indonesia |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Julie Legault | julie {at} amino {dot} bio | Canada |
| Ashton Cropp | tacropp {at} vcu {dot} edu | United States |
| Shivang Joshi | shivangjoshis4 {at} gmail {dot} com | United Kingdom |
| Jae-Seong Yang | jaeseong.yang {at} cragenomica {dot} es | Spain |
| Rodolfo Marcolino | rodolfo.marcolino {at} me {dot} com | Argentina |
| Vincent Paris | NA | United States |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| Jennifer Molloy | jcm80 {at} cam {dot} ac {dot} uk | United Kingdom |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| Libby Sharpe | libby.sharpe {at} leomail {dot} tamuc {dot} edu | United States |
| Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Franklin Nobrega | f.nobrega {at} soton {dot} ac {dot} uk | United Kingdom |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Arthur Zanetti Nunes Fernandes | fgueiros {at} iq {dot} usp {dot} br | United States |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Pakpoom Subsoontorn | pakpoomton {at} gmail {dot} com | Thailand |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| kangquan YIN | yinkq {at} bjfu {dot} edu {dot} cn | China |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Josephine Labos | N/A | Philippines |
| Maan Neamah | maanm.neamah {at} uokufa {dot} edu {dot} iq | Iraq |
| robson tramontina | robson.tramontina {at} gmail {dot} com | Brazil |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Benjamin Chavez | chavez {at} ipk-gatersleben {dot} de | Germany |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| mehmet tardu | mtardu {at} gmail {dot} com | Turkey |
| Wapouo Fadanka Stephane | mboalab {at} gmail {dot} com | Republic of Cameroon |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Fernan Federici | ffederici {at} bio {dot} puc {dot} cl | Chile |
| Yan Zhang | yz473 {at} gatech {dot} edu | United States |
Download all of this information as a CSV from our GitHub.