One-Step Cloning and Chromosomal Integration of DNA

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What is it?

“Clonetegration” is a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.

What can it be used for?

Integrating your favorite DNA into specific loci on a bacterial chromosome.

Where can I find more information?

One-Step Cloning and Chromosomal Integration of DNA, François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin, https://pubs.acs.org/doi/full/10.1021/sb400021j?src=recsys&

The One-Step Cloning and Chromosomal Integration of DNA Toolkit is shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Cresol Red will not impact plasmid transformation.

If you've already received the plate, use Chrome or Firefox to download the plate layout. Plates are labelled "One Step Integration Collection"

Genes

Gene	Name	Freegenes ID
pE-FLP	pE-FLP	BBF10K_000016
pOSIP-CH (CamR, HK022)	pOSIP-CH (CamR, HK022)	BBF10K_000031
pOSIP-KO (KanR, 186)	pOSIP-KO (KanR, 186)	BBF10K_000492
pOSIP-TT (TetR, P21)	pOSIP-TT (TetR, P21)	BBF10K_000493

Download all of this information as a CSV from our GitHub.

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name	Contact	Country
Xingyue jiang	karma5781 {at} 21cn {dot} com	China
John Allan	john.allan {at} northumbria {dot} ac {dot} uk	United Kingdom
Shivang Joshi	shivangjoshis4 {at} gmail {dot} com	United Kingdom
Maan Neamah	maanm.neamah {at} uokufa {dot} edu {dot} iq	Iraq
Shree Ram Singh	singhlabasu {at} gmail {dot} com	United States
Yan Zhang	yz473 {at} gatech {dot} edu	United States
Keoni Gandall	koeng101 {at} gmail {dot} com	United States
Kenneth Frimpong	ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh	Ghana
Ahmed Atef	ahmed_atefaig2 {at} yahoo {dot} com	Saudi Arabia
Mikhail Khvochtchev	mikhail.khv {at} mahidol {dot} edu	Thailand
kangquan YIN	yinkq {at} bjfu {dot} edu {dot} cn	China
Aleksandr Shilovich	mercurialbadger {at} yandex {dot} ru	Russia
Cauã Westman	caua.westmann {at} ieu {dot} uzh {dot} ch	Switzerland
Swaraj Kunal	swaraj.avisa {at} gmail {dot} com	India
Majed Alghamdi	mafa2 {at} le {dot} ac {dot} uk	United Kingdom
Benjamin Chavez	chavez {at} ipk-gatersleben {dot} de	Germany
Franklin Nobrega	f.nobrega {at} soton {dot} ac {dot} uk	United Kingdom
Ian Cubit	Djcubit {at} nycap {dot} rr {dot} com	United States
Ashton Cropp	tacropp {at} vcu {dot} edu	United States
Jae-Seong Yang	jaeseong.yang {at} cragenomica {dot} es	Spain
Kefentse Arnold Tumedi	tumediak {at} yahoo {dot} com	Botswana
Fernan Federici	ffederici {at} bio {dot} puc {dot} cl	Chile
Fristot Elsa	elsa.fristot {at} cbs {dot} cnrs {dot} fr	France
Juan Vicente Farizano	juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar	Argentina
Amjed Alsultan	NA	Iraq
Vincent Paris	NA	United States
Rodolfo Marcolino	rodolfo.marcolino {at} me {dot} com	Argentina
Jordan Gonzalez	jgonzalez {at} thecitizensciencelab {dot} org	United States
Erman ÖZER	aracnoid {at} gmail {dot} com	Turkey
Ahmad Suparmin	ahmad.suparmin {at} ugm {dot} ac {dot} id	Indonesia
Julie Legault	julie {at} amino {dot} bio	Canada
Han Teng Wong	wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg	Singapore
robson tramontina	robson.tramontina {at} gmail {dot} com	Brazil
Peer Schenk	reuben.brown {at} uqconnect {dot} edu {dot} au	Australia
Jose Munoz	Jose.Munoz {at} northumbria {dot} ac {dot} uk	United Kingdom
Simran Kushwaha	https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty	India
Ben Thuronyi	bwt2 {at} williams {dot} edu	United States
Phil Roche	philroche365 {at} gmail {dot} com	Canada
Ahmed Hegazy	a.hegazy {at} biotec {dot} rwth-aachen {dot} de	Germany
Jennifer Molloy	jcm80 {at} cam {dot} ac {dot} uk	United Kingdom
Kyle Erlenbeck	krerlenbeck {at} dcsdk12 {dot} org	United States

Download all of this information as a CSV from our GitHub.

Back to Endy Lab at Stanford University

MTA

Unilateral OpenMTA

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Material: Original Material, Progeny, and Unmodified Derivatives. The Material shall not include: (a) Modifications, or (b) other substances created by the Recipient through the use of the Material, which are not Modifications, Progeny, or Unmodified Derivatives.
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