What is it?
“Clonetegration” is a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.
What can it be used for?
Integrating your favorite DNA into specific loci on a bacterial chromosome.
Where can I find more information?
One-Step Cloning and Chromosomal Integration of DNA, François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin, https://pubs.acs.org/doi/full/10.1021/sb400021j?src=recsys&
The One-Step Cloning and Chromosomal Integration of DNA Toolkit is shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
If you've already received the plate, use Chrome or Firefox to download the plate layout. Plates are labelled "One Step Integration Collection"
This kit is included in kit plate 2 of the 2022 iGEM distribution. The plate map for kit plate 2 of the iGEM 2022 distribution can be found here.
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| pE-FLP | pE-FLP | BBF10K_000016 |
| pOSIP-CH (CamR, HK022) | pOSIP-CH (CamR, HK022) | BBF10K_000031 |
| pOSIP-KO (KanR, 186) | pOSIP-KO (KanR, 186) | BBF10K_000492 |
| pOSIP-TT (TetR, P21) | pOSIP-TT (TetR, P21) | BBF10K_000493 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Pakpoom Subsoontorn | pakpoomton {at} gmail {dot} com | Thailand |
| Maan Neamah | maanm.neamah {at} uokufa {dot} edu {dot} iq | Iraq |
| Ashton Cropp | tacropp {at} vcu {dot} edu | United States |
| Benjamin Chavez | chavez {at} ipk-gatersleben {dot} de | Germany |
| Wapouo Fadanka Stephane | mboalab {at} gmail {dot} com | Republic of Cameroon |
| Libby Sharpe | libby.sharpe {at} leomail {dot} tamuc {dot} edu | United States |
| Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| robson tramontina | robson.tramontina {at} gmail {dot} com | Brazil |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| John Allan | john.allan {at} eng {dot} ox {dot} ac {dot} uk | United Kingdom |
| Shivang Joshi | shivangjoshis4 {at} gmail {dot} com | United Kingdom |
| Rodolfo Marcolino | rodolfo.marcolino {at} me {dot} com | Argentina |
| Jae-Seong Yang | jaeseong.yang {at} cragenomica {dot} es | Spain |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| kangquan YIN | yinkq {at} bjfu {dot} edu {dot} cn | China |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Moritz Venne | moritz.venne {at} web {dot} de | Germany |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Amir Kashani | NA | Canada |
| Jonathan Jonathan | Jonathancontact16 {at} gmail {dot} com | Indonesia |
| Julie Legault | julie {at} amino {dot} bio | Canada |
| Franklin Nobrega | f.nobrega {at} soton {dot} ac {dot} uk | United Kingdom |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
| Yan Zhang | yz473 {at} gatech {dot} edu | United States |
| Fernan Federici | ffederici {at} bio {dot} puc {dot} cl | Chile |
| Meng Lu | menglu {at} iastate {dot} edu | United States |
| mehmet tardu | mtardu {at} gmail {dot} com | Turkey |
| Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Kyle Erlenbeck | krerlenbeck {at} dcsdk12 {dot} org | United States |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Josephine Labos | N/A | Philippines |
| Amjed Alsultan | NA | Iraq |
| Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Vincent Paris | NA | United States |
| Mikhail Khvochtchev | mikhail.khv {at} mahidol {dot} edu | Thailand |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Arthur Zanetti Nunes Fernandes | fgueiros {at} iq {dot} usp {dot} br | United States |
| Jennifer Molloy | jcm80 {at} cam {dot} ac {dot} uk | United Kingdom |
Download all of this information as a CSV from our GitHub.