COVID-19 Mammalian ORFs and Expression Vectors

COVID-19 Mammalian ORFs and Expression Vectors

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A collection of COVID-19 ORFs optimized for Mammalian expression. They come in two different formats, in expression vectors and in gateway compatible vectors.

Unlike most of the collections that FreeGenes offers, all vectors will be shipped as raw DNA. You do not need a -80C freezer to store this kit. If you are asked at checkout whether you possess a -80C freezer, you can still receive this kit if you answer 'no.'

These materials were created by the Krogan lab at UCSF and the Roth lab at the University of Toronto.

 

 

Documents: Platemap, Krogan Lab Mailing Slip, Plasmid Table

Please note that the well coordinates in the Krogan Lab Mailing Slip may not be accurate. Refer to the Plasmid Table for the correct well coordinates, and for individual plasmid maps with sequencing data.

Each well contains roughly 15 ng of DNA at a concentration of 5ng/uL. Please centrifuge the plate before puncturing or removing the foil cover.

This product is made available under the unilateral OpenMTASome or all of these items are for use only as permitted by a research exemption.

 

Genes

Gene Name NCBI ID Freegenes ID
SARS-CoV-2-nsp1 SARS-CoV-2-nsp1 YP_009725295.1 BBF10K_003511
SARS-CoV-2-nsp2 SARS-CoV-2-nsp2 YP_009725295.1 BBF10K_003512
SARS-CoV-2-nsp4 SARS-CoV-2-nsp4 YP_009725295.1 BBF10K_003513
SARS-CoV-2-nsp5 SARS-CoV-2-nsp5 YP_009725295.1 BBF10K_003514
SARS-CoV-2-nsp5-C145A (also called 3CLPro) SARS-CoV-2-nsp5-C145A (also called 3CLPro) YP_009725295.1 BBF10K_003515
SARS-CoV-2-nsp6 SARS-CoV-2-nsp6 YP_009725295.1 BBF10K_003516
SARS-CoV-2-nsp7 SARS-CoV-2-nsp7 YP_009725295.1 BBF10K_003517
SARS-CoV-2-nsp8 SARS-CoV-2-nsp8 YP_009725295.1 BBF10K_003518
SARS-CoV-2-nsp9 SARS-CoV-2-nsp9 YP_009725295.1 BBF10K_003519
SARS-CoV-2-nsp10 SARS-CoV-2-nsp10 YP_009725295.1 BBF10K_003520
SARS-CoV-2-nsp11 SARS-CoV-2-nsp11 YP_009725295.1 BBF10K_003521
SARS-CoV-2-nsp12 SARS-CoV-2-nsp12 YP_009725295.1 BBF10K_003522
SARS-CoV-2-nsp13 SARS-CoV-2-nsp13 YP_009725295.1 BBF10K_003523
SARS-CoV-2-nsp14 SARS-CoV-2-nsp14 YP_009725295.1 BBF10K_003524
SARS-CoV-2-nsp15 SARS-CoV-2-nsp15 YP_009725295.1 BBF10K_003525
SARS-CoV-2-orf7b SARS-CoV-2-orf7b YP_009725318.1 BBF10K_003526
SARS-CoV-2-S SARS-CoV-2-S YP_009724390.1 BBF10K_003527
SARS-CoV-2-orf3a SARS-CoV-2-orf3a YP_009724391.1 BBF10K_003528
SARS-CoV-2-orf3b SARS-CoV-2-orf3b BBF10K_003529
SARS-CoV-2-E SARS-CoV-2-E YP_009724392.1 BBF10K_003530
SARS-CoV-2-M SARS-CoV-2-M YP_009724393.1 BBF10K_003531
SARS-CoV-2-orf6 SARS-CoV-2-orf6 YP_009724394.1 BBF10K_003532
SARS-CoV-2-orf7a SARS-CoV-2-orf7a YP_009724395.1 BBF10K_003533
SARS-CoV-2-orf8 SARS-CoV-2-orf8 YP_009724396.1 BBF10K_003534
SARS-CoV-2-N SARS-CoV-2-N YP_009724397.2 BBF10K_003535
SARS-CoV-2-orf9b SARS-CoV-2-orf9b BBF10K_003536
SARS-CoV-2-orf9c SARS-CoV-2-orf9c BBF10K_003537
SARS-CoV-2-orf10 SARS-CoV-2-orf10 YP_009725255.1 BBF10K_003538
eGFP eGFP coded for mammalian expression BBF10K_003539
NSP2 NSP2 YP_009725295.1 BBF10K_003540
NSP4 NSP4 YP_009725295.1 BBF10K_003541
NSP5 NSP5 YP_009725295.1 BBF10K_003542
NSP6 NSP6 YP_009725295.1 BBF10K_003543
NSP7 NSP7 YP_009725295.1 BBF10K_003544
NSP8 NSP8 YP_009725295.1 BBF10K_003545
NSP9 NSP9 YP_009725295.1 BBF10K_003546
NSP10 NSP10 YP_009725295.1 BBF10K_003547
RNA-pol (NSP12) RNA-pol (NSP12) YP_009725295.1 BBF10K_003548
Heli (NSP13) Heli (NSP13) YP_009725295.1 BBF10K_003549
NSP14 NSP14 YP_009725295.1 BBF10K_003550
NSP15 NSP15 YP_009725295.1 BBF10K_003551
NSP16 NSP16 YP_009725295.1 BBF10K_003552
3b 3b BBF10K_003553
6 6 YP_009724394.1 BBF10K_003554
7b 7b YP_009725318.1 BBF10K_003555
8 8 YP_009724396.1 BBF10K_003556
9b 9b BBF10K_003557
NSP1 NSP1 YP_009725295.1 BBF10K_003558
PLPRO (NSP3) PLPRO (NSP3) YP_009725295.1 BBF10K_003559
3a 3a YP_009724391.1 BBF10K_003560
E E YP_009724392.1 BBF10K_003561
M M YP_009724393.1 BBF10K_003562
7a 7a YP_009724395.1 BBF10K_003563

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Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact Country
Lorenzo Zolfanelli biopunk {at} zolfa {dot} nl France
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org United States
Majed Alghamdi mafa2 {at} le {dot} ac {dot} uk United Kingdom
Tania Pozzo tania.pozzo {at} gmail {dot} com Sweden
Joey Riepsaame joey.riepsaame {at} path {dot} ox {dot} ac {dot} uk United Kingdom
Ahmed Atef ahmed_atefaig2 {at} yahoo {dot} com Saudi Arabia
Jimmy Gledson Hayden Linhares haydenlinhars {at} gmail {dot} com Brazil
Michael Chavez mgchavez {at} stanford {dot} edu United States
Louise Dalgaard ltd {at} ruc {dot} dk Denmark
Amitabha Majumdar Mamitava {at} nccs {dot} res {dot} in India
Kenneth Frimpong ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh Ghana
Vitaliy Strochkov NA Kazakhstan
Ery Fukushima ery.fukushima {at} ikiam {dot} edu {dot} ec Ecuador
Matthew Phanchana matthew.pha {at} mahidol {dot} edu Thailand
Hassan Awan Biologist.awan {at} gmail {dot} com Pakistan
Dong Nguyen Tam NA Vietnam
Amjed Alsultan amjed.talib {at} qu {dot} edu {dot} iq Iraq

Download all of this information as a CSV from our GitHub.
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