COVID-19 Mammalian ORFs and Expression Vectors

COVID-19 Mammalian ORFs and Expression Vectors

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A collection of COVID-19 ORFs optimized for Mammalian expression. They come in two different formats, in expression vectors and in gateway compatible vectors.

Unlike most of the collections that FreeGenes offers, all vectors will be shipped as raw DNA. You do not need a -80C freezer to store this kit. If you are asked at checkout whether you possess a -80C freezer, you can still receive this kit if you answer 'no.'

These materials were created by the Krogan lab at UCSF and the Roth lab at the University of Toronto.

 

 

Documents: Platemap, Krogan Lab Mailing Slip, Plasmid Table

Please note that the well coordinates in the Krogan Lab Mailing Slip may not be accurate. Refer to the Plasmid Table for the correct well coordinates, and for individual plasmid maps with sequencing data.

Each well contains roughly 15 ng of DNA at a concentration of 5ng/uL. Please centrifuge the plate before puncturing or removing the foil cover.

This product is made available under the unilateral OpenMTASome or all of these items are for use only as permitted by a research exemption.

 

Genes

Gene Name NCBI ID
SARS-CoV-2-nsp1 SARS-CoV-2-nsp1 YP_009725295.1
SARS-CoV-2-nsp2 SARS-CoV-2-nsp2 YP_009725295.1
SARS-CoV-2-nsp4 SARS-CoV-2-nsp4 YP_009725295.1
SARS-CoV-2-nsp5 SARS-CoV-2-nsp5 YP_009725295.1
SARS-CoV-2-nsp5-C145A (also called 3CLPro) SARS-CoV-2-nsp5-C145A (also called 3CLPro) YP_009725295.1
SARS-CoV-2-nsp6 SARS-CoV-2-nsp6 YP_009725295.1
SARS-CoV-2-nsp7 SARS-CoV-2-nsp7 YP_009725295.1
SARS-CoV-2-nsp8 SARS-CoV-2-nsp8 YP_009725295.1
SARS-CoV-2-nsp9 SARS-CoV-2-nsp9 YP_009725295.1
SARS-CoV-2-nsp10 SARS-CoV-2-nsp10 YP_009725295.1
SARS-CoV-2-nsp11 SARS-CoV-2-nsp11 YP_009725295.1
SARS-CoV-2-nsp12 SARS-CoV-2-nsp12 YP_009725295.1
SARS-CoV-2-nsp13 SARS-CoV-2-nsp13 YP_009725295.1
SARS-CoV-2-nsp14 SARS-CoV-2-nsp14 YP_009725295.1
SARS-CoV-2-nsp15 SARS-CoV-2-nsp15 YP_009725295.1
SARS-CoV-2-orf7b SARS-CoV-2-orf7b YP_009725318.1
SARS-CoV-2-S SARS-CoV-2-S YP_009724390.1
SARS-CoV-2-orf3a SARS-CoV-2-orf3a YP_009724391.1
SARS-CoV-2-orf3b SARS-CoV-2-orf3b
SARS-CoV-2-E SARS-CoV-2-E YP_009724392.1
SARS-CoV-2-M SARS-CoV-2-M YP_009724393.1
SARS-CoV-2-orf6 SARS-CoV-2-orf6 YP_009724394.1
SARS-CoV-2-orf7a SARS-CoV-2-orf7a YP_009724395.1
SARS-CoV-2-orf8 SARS-CoV-2-orf8 YP_009724396.1
SARS-CoV-2-N SARS-CoV-2-N YP_009724397.2
SARS-CoV-2-orf9b SARS-CoV-2-orf9b
SARS-CoV-2-orf9c SARS-CoV-2-orf9c
SARS-CoV-2-orf10 SARS-CoV-2-orf10 YP_009725255.1
eGFP eGFP coded for mammalian expression
NSP2 NSP2 YP_009725295.1
NSP4 NSP4 YP_009725295.1
NSP5 NSP5 YP_009725295.1
NSP6 NSP6 YP_009725295.1
NSP7 NSP7 YP_009725295.1
NSP8 NSP8 YP_009725295.1
NSP9 NSP9 YP_009725295.1
NSP10 NSP10 YP_009725295.1
RNA-pol (NSP12) RNA-pol (NSP12) YP_009725295.1
Heli (NSP13) Heli (NSP13) YP_009725295.1
NSP14 NSP14 YP_009725295.1
NSP15 NSP15 YP_009725295.1
NSP16 NSP16 YP_009725295.1
3b 3b
6 6 YP_009724394.1
7b 7b YP_009725318.1
8 8 YP_009724396.1
9b 9b
NSP1 NSP1 YP_009725295.1
PLPRO (NSP3) PLPRO (NSP3) YP_009725295.1
3a 3a YP_009724391.1
E E YP_009724392.1
M M YP_009724393.1
7a 7a YP_009724395.1

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact
Louise Dalgaard ltd {at} ruc {dot} dk
Vitaliy Strochkov NA
Amitabha Majumdar Mamitava {at} nccs {dot} res {dot} in
Dong Nguyen Tam NA
Amjed Alsultan amjed.talib {at} qu {dot} edu {dot} iq
Matthew Phanchana matthew.pha {at} mahidol {dot} edu
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org
Michael Chavez mgchavez {at} stanford {dot} edu
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