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Tropical Infectious Diseases Diagnostics and Therapeutics Research Toolkit

Tropical Infectious Diseases Diagnostics and Therapeutics Research Toolkit

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What is it?

Tropical infectious diseases present threats to tropical regions around the world; approximately 15 million people die every year as a result of infection from tropical infectious diseases and parasites. Research into diagnostic, therapeutic and vaccine development for these diseases are greatly needed, however expenses are limiting to researchers. We present this toolkit in support of fundamental tropical infectious disease research.

This toolkit contains S. cerevisiae codon-optimized genes encoding for envelope (E) and non-structural proteins (NS1) of major Flaviviruses, namely all four serotypes of Dengue, Zika and Japanese Encephalitis viruses (JEV). The encoded amino acid sequences have been selected to be the most prevalent variants of each type of virus. 

 

What can it be used for?

After being cloned into a yeast expression plasmid, these yeast optimized genes can be used to produce recombinant proteins in S. cerevisiae for a number of different purposes, including antigen, and/or antibody production. The antigens and antibodies can be used for basic research on viral pathogenesis, epitope mapping, and in developing novel diagnostic methods or novel vaccines against the Flaviviruses. They can also be used for educational purposes, such as demonstration of protein expression and purification in yeast, or engineering protein production through optimizing expression cassettes and/or expression conditions.

 

Notes: 

Plate map can be found here

Shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation. 

 

Where can I find more information?

The amino acid sequences can be found on NCBI Genpept using the following information:

Dengue virus 1 E protein (AQV12345.1 281-775)

Dengue virus 1 NS1 protein (AYP74685.1 776-1127)

Dengue virus 2 E protein (AYP74885.1 281-775)

Dengue virus 2 NS1 protein (AHG23160.1 776-1127)

Dengue virus 3 E protein (AFK83764.1 281-775)

Dengue virus 3 NS1 protein (AHG23254.1 774-1125)

Dengue virus 4 E protein (ACQ44401.1 280-774)

Dengue virus 4 NS1 protein (AFX65870.1 775-1126)

Zika virus E protein (AZS35375.1 291-794)

Zika virus NS1 protein (AAB18951.1 795-1146)

Japanese encephalitis virus E protein (AEO86789.1 295-794)

Japanese encephalitis virus N protein (AAB1895.1 795-1146)

 

The DNA sequences can be found on NCBI Nucleotide using the following information:

Yeast codon-optimized Dengue virus 1 Envelope glycoprotein (MW324565)

https://www.ncbi.nlm.nih.gov/nuccore/MW324565

Yeast codon-optimized Dengue virus 1 Non-structural protein 1 (MW324566)

https://www.ncbi.nlm.nih.gov/nuccore/MW324566

Yeast codon-optimized Dengue virus 2 Envelope glycoprotein (MW324567)

https://www.ncbi.nlm.nih.gov/nuccore/MW324567

Yeast codon-optimized Dengue virus 2 Non-structural protein 1 (MW324568)

https://www.ncbi.nlm.nih.gov/nuccore/MW324568

Yeast codon-optimized Dengue virus 3 Envelope glycoprotein (MW324569)

https://www.ncbi.nlm.nih.gov/nuccore/MW324569

Yeast codon-optimized Dengue virus 3 Non-structural protein 1 (MW324570)

https://www.ncbi.nlm.nih.gov/nuccore/MW324570

Yeast codon-optimized Dengue virus 4 Envelope glycoprotein (MW324571)

https://www.ncbi.nlm.nih.gov/nuccore/MW324571

Yeast codon-optimized Dengue virus 4 Nonstructural protein (MW324573)

https://www.ncbi.nlm.nih.gov/nuccore/MW324573

Yeast codon-optimized Zika virus Envelope glycoprotein (MW324573)

https://www.ncbi.nlm.nih.gov/nuccore/MW324573

Yeast codon-optimized Zika virus Non-structural protein 1 (MW324574)

https://www.ncbi.nlm.nih.gov/nuccore/MW324574

Yeast codon-optimized Japanese Encephalitis Virus Envelope glycoprotein (MW324575)

https://www.ncbi.nlm.nih.gov/nuccore/MW324575

Yeast codon-optimized Japanese Encephalitis Virus Non-structural glycoprotein (MW324576)

https://www.ncbi.nlm.nih.gov/nuccore/MW324576

 

Image attributed to Cornell University – PJ Mode Collection of Persuasive Cartography

Genes

Gene Name NCBI ID Freegenes ID
yD1E S. cerevisiae codon optimized Dengue serotype 1 Envelope glycoprotein UFQ72648 BBF10K_000590
yD1NS1 S. cerevisiae codon optimized Dengue serotype 1 Non-structural protein UFQ72649.1 BBF10K_000591
yD2E S. cerevisiae codon optimized Dengue serotype 2 Envelope glycoprotein UFQ72650.1 BBF10K_000592
yD2NS1 S. cerevisiae codon optimized Dengue serotype 2 non-structural protein 1 UFQ72651.1 BBF10K_000593
yD3E S. cerevisiae codon optimized Dengue serotype 3 Envelope protein UFQ72652.1 BBF10K_000594
yD3NS1 S. cerevisiae codon optimized Dengue serotype 3 Non-structural protein 1 UFQ72653.1 BBF10K_000595
yD4E S. cerevisiae codon optimized Dengue serotype 4 Envelope glycoprotein UFQ72654.1 BBF10K_000596
yD4NS1 S. cerevisiae codon optimized Dengue serotype 4 Non UFQ72655.1 BBF10K_000597
ZE S. cerevisiae codon optimized Zika virus Envelope glycoprotein UFQ72656.1 BBF10K_000598

Download all of this information as a CSV from our GitHub.

Bionet

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Download all of this information as a CSV from our GitHub.
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