The Open Reporter Collection includes 32 commonly used reporter genes used in all kinds of biology labs for a variety of techniques including measuring gene expression, diagnostics, verifying successful transformations and more. The collection includes chromoproteins (which produce a colour that is visible to the naked eye), fluorescent proteins (that require excitation by a certain wavelength of light and emit another wavelength), luciferases (which emit bioluminscence) and other enzymes that enable colorimetric detection of a substrate by changing its color, for example horseradish peroxidase which classically turns a chemical substrate bright pink in very common antibody-based assays such as ELISA.
What can the collection be used for?
There are so many uses for reporter genes that parts of this collection will be useful for almost any lab performing cell or molecular biology. We have provided reporters with a variety of spectra, output and maturation times to cater for specific research uses. Importantly, we cover a wide variety of colours that are visible to the naked eye and using fluorescent detection, making this collection useful not only for research but also for education and even bioart. All reporters are off-patent and can also be used commercially, which is good news for small biotech companies.
Open Reporters are not provided in expression vectors. In order to see a color, you must subclone your gene of interest into an expression vector.
More information can be found at the Open Bioeconomy Lab Website. A plate map can be found here.
This kit is included in kit plate 2 of the 2022 iGEM distribution. The plate map for kit plate 2 of the iGEM 2022 distribution can be found here.
This product is made available under the unilateral OpenMTA.
Open Reporters is shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
Prior Kit Versions
If you received an earlier version of this kit labeled "Open Enzymes (Part 2)/Open Reporters, you can find the platemap here.
Genes
| Gene | Name | Freegenes ID |
|---|---|---|
| cjBlue/ BioBrick K592011 | cjBlue/ BioBrick K592011 | BBF10K_003331 |
| gfasPurple/gfasCP Biobrick K1033918 | gfasPurple/gfasCP Biobrick K1033918 | BBF10K_003335 |
| meffCP | meffCP | BBF10K_003338 |
| meffCFP | meffCFP | BBF10K_003342 |
| mmilCFP | mmilCFP | BBF10K_003343 |
| meleCFP | meleCFP | BBF10K_003344 |
| efasCFP | efasCFP | BBF10K_003345 |
| aacuGFP1 | aacuGFP1 | BBF10K_003346 |
| aacuGFP2 | aacuGFP2 | BBF10K_003347 |
| afraGFP | afraGFP | BBF10K_003348 |
| amilGFP Biobrick K592010 | amilGFP Biobrick K592010 | BBF10K_003349 |
| eechGFP1 | eechGFP1 | BBF10K_003350 |
| eechGFP2 | eechGFP2 | BBF10K_003351 |
| eechGFP3 | eechGFP3 | BBF10K_003352 |
| efasGFP | efasGFP | BBF10K_003353 |
| gfasGFP | gfasGFP | BBF10K_003354 |
| palmGFP | palmGFP | BBF10K_003355 |
| amilRFP | amilRFP | BBF10K_003357 |
| meffRed/meffRFP Biobricks K592012 | meffRed/meffRFP Biobricks K592012 | BBF10K_003358 |
| meleRFP | meleRFP | BBF10K_003360 |
| scubRFP | scubRFP | BBF10K_003361 |
| eforRed/eforCP Biobrick K592012 | eforRed/eforCP Biobrick K592012 | BBF10K_003362 |
| amajLime/amajCFP/amFP486 BioBrick K1033916 | amajLime/amajCFP/amFP486 BioBrick K1033916 | BBF10K_003364 |
| EiraCFP Biobrick J97000 | EiraCFP Biobrick J97000 | BBF10K_003365 |
| HRP | horseradish peroxidase (HRP) | BBF10K_003367 |
| Firefly Luciferase [Photinus pyralis] (Fluc) | Firefly Luciferase [Photinus pyralis] (Fluc) | BBF10K_003368 |
| Luciferase [Luciola cruciata] (Japanese firefly) | Luciferase [Luciola cruciata] (Japanese firefly) | BBF10K_003369 |
| Renilla luciferase | Renilla luciferase | BBF10K_003370 |
| beta-lactamase | beta-lactamase | BBF10K_003371 |
| CIAP | Calf Intestine Alkaline phosphatase (CIAP) | BBF10K_003372 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
| Amir Kashani | NA | Canada |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| SERV0223789 CAROLINE BACQUET | caroline.bacquet {at} ikiam {dot} edu {dot} ec | United States |
| Gülşen Günel | gulsen.gunel {at} ogr {dot} iu {dot} edu {dot} tr | Turkey |
| Jose David Rosales | https://www.linkedin.com/in/david-rosales-574a7151/ | United States |
| Benjamin Loang | benjamin.liang {at} uq {dot} edu {dot} au | Australia |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Carlos Ueira-Vieira | ueira {at} ufu {dot} br | Brazil |
| Anil Challa | akchalla {at} uab {dot} edu | United States |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| James Altamirano | James_altam {at} utexas {dot} edu | United States |
| Joey Riepsaame | joey.riepsaame {at} path {dot} ox {dot} ac {dot} uk | United Kingdom |
| Lisandro Pacheco | lpacheco28 {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Jorge Marchand | jmarcha {at} uw {dot} edu | United States |
| Maira Goytia | mgoytia {at} spelman {dot} edu | United States |
| Leandro Oliveira (NG) | ll637 {at} cam {dot} ac {dot} uk | United Kingdom |
| Arthur Zanetti Nunes Fernandes | fgueiros {at} iq {dot} usp {dot} br | United States |
| Vitaliy Strochkov | NA | Kazakhstan |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| Moritz Venne | moritz.venne {at} web {dot} de | Germany |
| Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
| Elizabeth Bilsland | bilsland {at} unicamp {dot} br | Brazil |
| Tocvic Meng | Tocvic Meng \ Email: tocvic.meng {at} holocyte {dot} com \ website https://gmexpression {dot} com/ | Australia |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Jianxiang Shi | jianxiangshi {at} zzu {dot} edu {dot} cn | China |
| Joao Vitor Dutra Molino | candidomolino {at} gmail {dot} com | United States |
| Nikolaus Dietz | nikolaus.dietz {at} unibas {dot} ch | Switzerland |
| Lorenzo Zolfanelli | biopunk {at} zolfa {dot} nl | France |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
| Kevin Correia | kevin {at} kedalionlabs {dot} com | Netherlands |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Emmanuele Severi | emmanuele.severi {at} newcastle {dot} ac {dot} uk | United Kingdom |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Mark Slabodnick | mmslabodnick {at} knox {dot} edu | United States |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| John Allan | john.allan {at} eng {dot} ox {dot} ac {dot} uk | United Kingdom |
| Elena Rosca | erosca {at} ashesi {dot} edu {dot} gh | Ghana |
| mehmet tardu | mtardu {at} gmail {dot} com | Turkey |
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| Watanyu Bunsermyos | watanyu.bu {at} ku {dot} th | Thailand |
| Vincent Paris | NA | United States |
| Hernán Rebolledo | lenaranjo {at} gmail {dot} com | United States |
| Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| Elliot Medina | elliot.medina {at} moffitt {dot} org | United States |
| Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Sarah Ware | sarah.ware {at} bioblaze {dot} org | United States |
| Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Kelly Gallagher | kelly.gallagher {at} oneonta {dot} edu | United States |
| Emin Bursa | NA | United States |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Dr. Rajashree Patil | rajshree.patil.n {at} gmail {dot} com | India |
| robson tramontina | robson.tramontina {at} gmail {dot} com | Brazil |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | United States |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Josephine Labos | N/A | Philippines |
| Intan Taufik | i.taufik {at} sith {dot} itb {dot} ac {dot} id | Indonesia |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Jehovani Lopez | jlopez {at} genspace {dot} org | United States |
| Fernan Federici | ffederici {at} bio {dot} puc {dot} cl | Chile |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| Yi Wu | F.Nobrega {at} soton {dot} ac {dot} uk | United Kingdom |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Kristel Alman | kristel.alman {at} ut {dot} ee | Estonia |
| Chris Badenhorst | chris.badenhorst {at} uni-greifswald {dot} de | Germany |
| Nadia Odaliz Chamana Chura | nadia.chamana {at} utec {dot} edu {dot} pe | France |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
Download all of this information as a CSV from our GitHub.