Open Reporters

Open Reporters

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The Open Reporter Collection includes 42 commonly used reporter genes used in all kinds of biology labs for a variety of techniques including measuring gene expression, diagnostics, verifying successful transformations and more. The collection includes chromoproteins (which produce a colour that is visible to the naked eye), fluorescent proteins (that require excitation by a certain wavelength of light and emit another wavelength), luciferases (which emit bioluminscence) and other enzymes that enable colorimetric detection of a substrate by changing its color, for example horseradish peroxidase which classically turns a chemical substrate bright pink in very common antibody-based assays such as ELISA.

What can the collection be used for?
There are so many uses for reporter genes that parts of this collection will be useful for almost any lab performing cell or molecular biology. We have provided reporters with a variety of spectra, output and maturation times to cater for specific research uses. Importantly, we cover a wide variety of colours that are visible to the naked eye and using fluorescent detection, making this collection useful not only for research but also for education and even bioart. All reporters are off-patent and can also be used commercially, which is good news for small biotech companies. 

Open Reporters are not provided in expression vectors. In order to see a color, you must subclone your gene of interest into an expression vector.

More information can be found at the Open Bioeconomy Lab Website. A plate map can be found here  

This product is made available under the unilateral OpenMTA.  

Some or all of these items are for use only as permitted by a research exemption.

 

Open Reporters is available in two formats: E. coli glycerol stocks and purified nucleic acids. Please specify in the order notes if you need a particular format. 

 

Prior Kit Versions   

If you received an earlier version of this kit labeled "Open Enzymes (Part 2)/Open Reporters, you can find the platemap here.

 

 Genes

Gene Name Freegenes ID
cjBlue/ BioBrick K592011 cjBlue/ BioBrick K592011 BBF10K_003331
spisPink/spisCP Biobrick K1033925 spisPink/spisCP Biobrick K1033925 BBF10K_003333
amilCP Biobrick K592009 amilCP Biobrick K592009 BBF10K_003334
gfasPurple/gfasCP Biobrick K1033918 gfasPurple/gfasCP Biobrick K1033918 BBF10K_003335
ahyaCP ahyaCP BBF10K_003336
gdjiCP gdjiCP BBF10K_003337
meffCP meffCP BBF10K_003338
fuGFP fuGFP BBF10K_003339
amilCFP amilCFP BBF10K_003340
anobCFP2 anobCFP2 BBF10K_003341
meffCFP meffCFP BBF10K_003342
mmilCFP mmilCFP BBF10K_003343
meleCFP meleCFP BBF10K_003344
efasCFP efasCFP BBF10K_003345
aacuGFP1 aacuGFP1 BBF10K_003346
aacuGFP2 aacuGFP2 BBF10K_003347
afraGFP afraGFP BBF10K_003348
amilGFP Biobrick K592010 amilGFP Biobrick K592010 BBF10K_003349
eechGFP1 eechGFP1 BBF10K_003350
eechGFP2 eechGFP2 BBF10K_003351
eechGFP3 eechGFP3 BBF10K_003352
efasGFP efasGFP BBF10K_003353
gfasGFP gfasGFP BBF10K_003354
palmGFP palmGFP BBF10K_003355
amilRFP amilRFP BBF10K_003357
meffRed/meffRFP Biobricks K592012 meffRed/meffRFP Biobricks K592012 BBF10K_003358
meleRFP meleRFP BBF10K_003360
scubRFP scubRFP BBF10K_003361
eforRed/eforCP Biobrick K592012 eforRed/eforCP Biobrick K592012 BBF10K_003362
asPink/asFP595/asCP BioBrick K1033933 asPink/asFP595/asCP BioBrick K1033933 BBF10K_003363
amajLime/amajCFP/amFP486 BioBrick K1033916 amajLime/amajCFP/amFP486 BioBrick K1033916 BBF10K_003364
EiraCFP Biobrick J97000 EiraCFP Biobrick J97000 BBF10K_003365
HRP horseradish peroxidase (HRP) BBF10K_003367
Firefly Luciferase [Photinus pyralis] (Fluc) Firefly Luciferase [Photinus pyralis] (Fluc) BBF10K_003368
Luciferase [Luciola cruciata] (Japanese firefly) Luciferase [Luciola cruciata] (Japanese firefly) BBF10K_003369
Renilla luciferase Renilla luciferase BBF10K_003370
beta-lactamase beta-lactamase BBF10K_003371
CIAP Calf Intestine Alkaline phosphatase (CIAP) BBF10K_003372

Download all of this information as a CSV from our GitHub.

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact Country
Kefentse Arnold Tumedi tumediak {at} yahoo {dot} com Botswana
Ahmad Suparmin ahmad.suparmin {at} ugm {dot} ac {dot} id Indonesia
Phil Roche philroche365 {at} gmail {dot} com Canada
Vitaliy Strochkov NA Kazakhstan
Shree Ram Singh singhlabasu {at} gmail {dot} com United States
Xingyue jiang karma5781 {at} 21cn {dot} com China
Esteban Erben eerben {at} iib {dot} unsam {dot} edu {dot} ar Argentina
Yi Wu F.Nobrega {at} soton {dot} ac {dot} uk United Kingdom
Ian Cubit Djcubit {at} nycap {dot} rr {dot} com United States
Juan Vicente Farizano juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar Argentina
Ahmed Hegazy a.hegazy {at} biotec {dot} rwth-aachen {dot} de Germany
Ben Thuronyi bwt2 {at} williams {dot} edu United States
Jose David Rosales jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ Venezuela
Matthew Phanchana matthew.pha {at} mahidol {dot} edu Thailand
Emin Bursa NA United States
Swaraj Kunal swaraj.avisa {at} gmail {dot} com India
Keoni Gandall koeng101 {at} gmail {dot} com United States
Cauã Westman caua.westmann {at} ieu {dot} uzh {dot} ch Switzerland
Amitabha Majumdar Mamitava {at} nccs {dot} res {dot} in India
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org United States
Carlos Ueira-Vieira ueira {at} ufu {dot} br Brazil
John Allan john.allan {at} northumbria {dot} ac {dot} uk United Kingdom
Jimmy Linhares Synbioamazonas {at} gmail {dot} com Brazil
Angelica Hernandez sofiash.me {at} gmail {dot} com Mexico
Joey Riepsaame joey.riepsaame {at} path {dot} ox {dot} ac {dot} uk United Kingdom
Wei-Min Chang WeiMinChang {at} tmu {dot} edu {dot} tw Taiwan
Oskar Zeballos zebsamosk {at} gmail {dot} com Bolivia
Majed Alghamdi mafa2 {at} le {dot} ac {dot} uk United Kingdom
Sarah Ware sarah.ware {at} bioblaze {dot} org United States
Han Teng Wong wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg Singapore
James Altamirano James_altam {at} utexas {dot} edu United States
Tim Dobbs tim.dobbs {at} cri-paris {dot} org France
Aleksandr Shilovich mercurialbadger {at} yandex {dot} ru Russia
Kenneth Frimpong ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh Ghana
Pratik Vyas iampratikvyas {at} gamil {dot} com India
Lorenzo Zolfanelli biopunk {at} zolfa {dot} nl France

Download all of this information as a CSV from our GitHub.
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