
Currently, sharing and reuse of plasmids is managed by material transfer agreements and patent-based property rights. Navigating these rights and agreements can place cumbersome legal burdens on researchers, who often do not have the legal training to negotiate the agreements themselves, and who may not have access to a university technology transfer office who can help them through the process.
To address these challenges, we have made our own open source plasmids of known provenance and free of IP conflict, thus removing property rights encumbrances and redistribution barriers. Here we present the Open Plasmids Collection, a growing group of standard, freely available, open-source plasmids. Plasmids in the collection can be reused, remade, and redistributed as often as one would like.
What can it be used for:
The Open Plasmids distribution is comprised of three plasmids: pBuild, pYeast, and pInducible. Each vector was designed specifically for the Open Plasmids Collection, and has both an E. coli origin of replication and an antibiotic resistance marker for negative selection.
pBuild is designed as a robust, flexible E. coli cloning vector. The plasmid backbone is compatible with multiple cloning strategies, including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard. pYeast is an expression vector designed for propagation in E. coli and galactose-inducible expression in yeast, allowing users to investigate their genes in a eukaryotic environment. pInducible is an inducible-copy vector based on iGEM plasmid pSB2K3, and intended for cloning difficult or toxic genes.
Where can I find more information:
- More information about plasmids
- Freegenes Standard MoClo sites
- Plate Layout
- pBuild genbank file
- pYeast genbank file
- pInducible genbank file
Plates in distribution: 1
Shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
Plate layout can be found here.
Instructions for Use:
Transform your plasmid of choice into competent Top10 E. coli cells (or similar). Culture at 37C overnight, with shaking at around 300rpm. The bacterial selection marker for pBuild is ampicillin, and the bacterial selection marker for pYeast and pInducible is kanamycin.
To induce pInducible’s high copy origin of replication, add IPTG to the mid-log-phase culture to a final concentration of 1mM. Allow cells to grow to saturation, then miniprep DNA.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
Genes
Gene | Name | NCBI ID | Freegenes ID |
---|---|---|---|
pBuild | pOpen_v3 | N/A | BBF10K_003498 |
pYeast | pOpen_yeast | N/A | BBF10K_003499 |
pInducible | pOpen_v4 | N/A | BBF10K_000589 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.Name | Contact | Country |
---|---|---|
Stephen Klusza | smklusza {at} gmail {dot} com | United States |
Aileen Button | acbutton {at} ucsd {dot} edu | United States |
Ed Eisenstein | eisenste {at} umd {dot} edu | United States |
Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
Gülşen Günel | gulsen.gunel {at} ogr {dot} iu {dot} edu {dot} tr | Turkey |
Jonathan Jonathan | Jonathancontact16 {at} gmail {dot} com | Indonesia |
Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
Anil Challa | akchalla {at} uab {dot} edu | United States |
Peter Rootes | root0059 {at} umn {dot} edu | United States |
Miles Rogers | miles.rogers {at} raytheon {dot} com | United States |
James Altamirano | James_altam {at} utexas {dot} edu | United States |
Ian Cubit | Djcubit {at} nycap {dot} are {dot} com | United States |
Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
Maira Goytia | mgoytia {at} spelman {dot} edu | United States |
Vitaliy Strochkov | NA | Kazakhstan |
Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
Rolando Perez | rcperez {at} alumni {dot} stanford {dot} edu | United States |
Elizabeth Bilsland | bilsland {at} unicamp {dot} br | Brazil |
Andrei Berasneu | a.berasneu {at} kleverlab {dot} pl | Poland |
Tocvic Meng | Tocvic Meng \ Email: tocvic.meng {at} holocyte {dot} com \ website https://gmexpression {dot} com/ | Australia |
Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
Dong Nguyen Tam | NA | Vietnam |
Joao Vitor Dutra Molino | candidomolino {at} gmail {dot} com | United States |
Nikolaus Dietz | nikolaus.dietz {at} unibas {dot} ch | Switzerland |
Lorenzo Zolfanelli | biopunk {at} zolfa {dot} nl | France |
Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
Kevin Correia | kevin {at} kedalionlabs {dot} com | Netherlands |
Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
Matias Cabeza | matiascabeza {at} gmail {dot} com | Argentina |
Dayananda Chandrappa | nanda.daya {at} gmail {dot} com | India |
Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
Stephen Klusza | StephenKlusza {at} clayton {dot} edu | United States |
Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
mehmet tardu | mtardu {at} gmail {dot} com | Turkey |
Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
Alexandr Dorif | dorif11 {at} gmail {dot} com | Moldova, Republic of |
Vincent Paris | NA | United States |
Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
Hernán Rebolledo | lenaranjo {at} gmail {dot} com | United States |
Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
Danielle Pedrolli | danielle.pedrolli {at} unesp {dot} br | Brazil |
Shivang Joshi | https://www.bacto.bio | United Kingdom |
Phil Roche | philroche365 {at} gmail {dot} com | Canada |
Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
raghav sridhar | raghav {at} cambrianbioworks {dot} com | India |
Rodrigo Caroca | rcaroca {at} uazuay {dot} edu {dot} ec | Ecuador |
Kheng Oon Low | khengoon {at} nibm {dot} my | Malaysia |
Amjed Alsultan | NA | Iraq |
Ben Davis | bed58 {at} aber {dot} ac {dot} uk | United Kingdom |
Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
Maan Neamah | maanm.neamah {at} uokufa {dot} edu {dot} iq | Iraq |
Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
Mariela Escobar | maru {at} michroma {dot} co | Argentina |
Emin Bursa | NA | United States |
Julio Bonilla | jabonill {at} espol {dot} edu {dot} ec | Ecuador |
Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
Dr. Rajashree Patil | rajshree.patil.n {at} gmail {dot} com | India |
Keoni Gandall | keonigandall.com | United States |
Keoni Gandall | keoni {at} sporenetlabs {dot} com | United States |
Wilson Teran | wteran.doc {at} gmail {dot} com | Colombia |
Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
Josephine Labos | N/A | Philippines |
Johan Sosa | johan {at} thefunquantum {dot} com | United States |
Tania Pozzo | tania.pozzo {at} gmail {dot} com | Sweden |
Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
Jae-Seong Yang | jaeseong.yang {at} cragenomica {dot} es | Spain |
Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
semih cagan | semihcgn {at} gmail {dot} com | Turkey |
Norhan Hassan | norhan.hassan {at} kaust {dot} edu {dot} sa | Saudi Arabia |
Kristel Alman | kristel.alman {at} ut {dot} ee | Estonia |
Nadia Odaliz Chamana Chura | nadia.chamana {at} utec {dot} edu {dot} pe | France |
Scott Pownall | scott {at} opensciencenet {dot} org | Canada |
Travis Wertz | travis {at} traviswertz {dot} com | United States |
Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
Download all of this information as a CSV from our GitHub.