Currently, sharing and reuse of plasmids is managed by material transfer agreements and patent-based property rights. Navigating these rights and agreements can place cumbersome legal burdens on researchers, who often do not have the legal training to negotiate the agreements themselves, and who may not have access to a university technology transfer office who can help them through the process.
To address these challenges, we have made our own open source plasmids of known provenance and free of IP conflict, thus removing property rights encumbrances and redistribution barriers. Here we present the Open Plasmids Collection, a growing group of standard, freely available, open-source plasmids. Plasmids in the collection can be reused, remade, and redistributed as often as one would like.
What can it be used for:
The Open Plasmids distribution is comprised of three plasmids: pBuild, pYeast, and pInducible. Each vector was designed specifically for the Open Plasmids Collection, and has both an E. coli origin of replication and an antibiotic resistance marker for negative selection.
pBuild is designed as a robust, flexible E. coli cloning vector. The plasmid backbone is compatible with multiple cloning strategies, including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard. pYeast is an expression vector designed for propagation in E. coli and galactose-inducible expression in yeast, allowing users to investigate their genes in a eukaryotic environment. pInducible is an inducible-copy vector based on iGEM plasmid pSB2K3, and intended for cloning difficult or toxic genes.
Where can I find more information:
- More information about plasmids
- Freegenes Standard MoClo sites
- Plate Layout
- pBuild genbank file
- pYeast genbank file
- pInducible genbank file
Plates in distribution: 1
Shipped as purified and dried down plasmid DNA stained with Cresol Red in one 96-well plate. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
Plate layout can be found here.
Instructions for Use:
Transform your plasmid of choice into competent Top10 E. coli cells (or similar). Culture at 37C overnight, with shaking at around 300rpm. The bacterial selection marker for pBuild is ampicillin, and the bacterial selection marker for pYeast and pInducible is kanamycin.
To induce pInducible’s high copy origin of replication, add IPTG to the mid-log-phase culture to a final concentration of 1mM. Allow cells to grow to saturation, then miniprep DNA.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
Genes
| Gene | Name | NCBI ID | Freegenes ID |
|---|---|---|---|
| pBuild | pOpen_v3 | N/A | BBF10K_003498 |
| pYeast | pOpen_yeast | N/A | BBF10K_003499 |
| pInducible | pOpen_v4 | N/A | BBF10K_000589 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Sewa Singh | sewasingh {at} rnavaxbio {dot} com | India |
| Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
| Gülşen Günel | gulsen.gunel {at} ogr {dot} iu {dot} edu {dot} tr | Turkey |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| Wilson Teran | wteran.doc {at} gmail {dot} com | Colombia |
| Miles Rogers | miles.rogers {at} raytheon {dot} com | United States |
| Hernán Rebolledo | lenaranjo {at} gmail {dot} com | United States |
| Vitaliy Strochkov | NA | Kazakhstan |
| Stephen Klusza | StephenKlusza {at} clayton {dot} edu | United States |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Dayananda Chandrappa | nanda.daya {at} gmail {dot} com | India |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Danielle Pedrolli | danielle.pedrolli {at} unesp {dot} br | Brazil |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Ben Davis | bed58 {at} aber {dot} ac {dot} uk | United Kingdom |
| Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
| Emin Bursa | NA | United States |
| Maira Goytia | mgoytia {at} spelman {dot} edu | United States |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Ian Cubit | Djcubit {at} nycap {dot} are {dot} com | United States |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Norhan Hassan | norhan.hassan {at} kaust {dot} edu {dot} sa | Saudi Arabia |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| mehmet tardu | mtardu {at} gmail {dot} com | Turkey |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Jose David Rosales | jdrr55 {at} yahoo {dot} com https://www {dot} linkedin {dot} com/in/david-rosales-574a7151/ | Venezuela |
| Tocvic Meng | Tocvic Meng \ Email: tocvic.meng {at} holocyte {dot} com \ website https://gmexpression {dot} com/ | Australia |
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Phil Roche | philroche365 {at} gmail {dot} com | Canada |
| Alexandr Dorif | dorif11 {at} gmail {dot} com | Moldova, Republic of |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| Nadia Odaliz Chamana Chura | nadia.chamana {at} utec {dot} edu {dot} pe | France |
| Andrei Berasneu | a.berasneu {at} kleverlab {dot} pl | Poland |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Kristel Alman | kristel.alman {at} ut {dot} ee | Estonia |
| Matias Cabeza | matiascabeza {at} gmail {dot} com | Argentina |
| Ian Cubit | Djcubit {at} nycap {dot} rr {dot} com | United States |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| semih cagan | semihcgn {at} gmail {dot} com | Turkey |
| Simon Hofer | soyouwantalab {at} gmail {dot} com | Austria |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Amjed Alsultan | NA | Iraq |
| Nikolaus Dietz | nikolaus.dietz {at} unibas {dot} ch | Switzerland |
| Vincent Paris | NA | United States |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| James Altamirano | James_altam {at} utexas {dot} edu | United States |
| Anil Challa | akchalla {at} uab {dot} edu | United States |
| Ed Eisenstein | eisenste {at} umd {dot} edu | United States |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Lorenzo Zolfanelli | biopunk {at} zolfa {dot} nl | France |
| Jae-Seong Yang | jaeseong.yang {at} cragenomica {dot} es | Spain |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| Tal Globus | bio {at} talglobus {dot} com | United States |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Tania Pozzo | tania.pozzo {at} gmail {dot} com | Sweden |
| Elizabeth Bilsland | bilsland {at} unicamp {dot} br | Brazil |
| Joao Vitor Dutra Molino | candidomolino {at} gmail {dot} com | United States |
| Travis Wertz | travis {at} traviswertz {dot} com | United States |
| Keoni Gandall | keonigandall.com | United States |
| Scott Pownall | scott {at} opensciencenet {dot} org | Canada |
| Kevin Correia | kevin {at} kedalionlabs {dot} com | Netherlands |
| Johan Sosa | johan {at} thefunquantum {dot} com | United States |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| Rolando Perez | rcperez {at} alumni {dot} stanford {dot} edu | United States |
| Cauã Westman | caua.westmann {at} ieu {dot} uzh {dot} ch | Switzerland |
| Maan Neamah | maanm.neamah {at} uokufa {dot} edu {dot} iq | Iraq |
| Julio Bonilla | jabonill {at} espol {dot} edu {dot} ec | Ecuador |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| Keoni Gandall | keoni {at} sporenetlabs {dot} com | United States |
| Kheng Oon Low | khengoon {at} nibm {dot} my | Malaysia |
| Dr. Rajashree Patil | rajshree.patil.n {at} gmail {dot} com | India |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| Oskar Zeballos | zebsamosk {at} gmail {dot} com | Bolivia |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Jonathan Jonathan | Jonathancontact16 {at} gmail {dot} com | Indonesia |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Dong Nguyen Tam | NA | Vietnam |
| Josephine Labos | N/A | Philippines |
| Xingyue jiang | karma5781 {at} 21cn {dot} com | China |
| raghav sridhar | raghav {at} cambrianbioworks {dot} com | India |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Rodrigo Caroca | rcaroca {at} uazuay {dot} edu {dot} ec | Ecuador |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
Download all of this information as a CSV from our GitHub.