Mycoplasma pneumoniae genes (E. coli codon optimized)

Mycoplasma pneumoniae genes (E. coli codon optimized)

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Mycoplasma pneumoniae is a close relative to Mycoplasma mycoides and Mycoplasma genitalium, and is known to cause disease in humans. Being able to rapidly test differences between those 3 genomes is necessary for synthetic genome research, and the genes themselves are important for biomedical research to find new drug targets. However, there is no resource for Mycoplasma pneumoniae genes to conduct this research. To enable comparative synthetic genome research and biomedical advancements, FreeGenes has synthesized 189 of 690 Mycoplasma pneumoniae M129 genes, codon optimized for Escherichia coli. Hopefully, by supporting Mycoplasma research, FreeGenes can encourage synthetic biology laboratories to conduct synthetic genome research.

What is it
This is a collection of as many protein coding sequences from Mycoplasma pneumoniae M129 as FreeGenes could clone in a single round of cloning. They are all standardized for MoClo assembly and codon optimized for Escherichia coli.

What can it be used for
There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Mycoplasma genome.

All genes are present on the plate in E. coli glycerol stocks. This product is made available under the unilateral OpenMTA

Where I can find more information

189 unique parts

Instructions for Use:

Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.

PlateSet: Mpneumonia-0 Mpneumonia-1

Plate Layout



Gene Name NCBI ID
xdj1 DnaJ domain-containing protein NP_109690.1
yabD deoxyribonuclease NP_109697.1
MPN013 hypothetical protein NP_109701.1
rpoE DNA-directed RNA polymerase subunit delta NP_109712.1
MPN032 hypothetical protein NP_109720.1
upp uracil phosphoribosyltransferase NP_109721.1
MPN038 hypothetical protein NP_109726.1
glpF glycerol uptake facilitator NP_109731.1
MPN047 nicotinate phosphoribosyltransferase NP_109735.1
glpK glycerol kinase NP_109738.1
MPN054 hypothetical protein NP_109742.1
potB spermidine/putrescine transport system permease NP_109744.1
gcp tRNA N6-adenosine threonylcarbamoyltransferase NP_109747.1
metX S-adenosylmethionine synthase NP_109748.1
deoD purine nucleoside phosphorylase NP_109750.1
deoA thymidine phosphorylase NP_109752.1
yabF ribonuclease M5 NP_109760.1
fruK 1-phosphofructokinase NP_109767.1
MPN083 lipoprotein NP_109771.1
MPN085 hypothetical protein NP_109773.1
MPN087 hypothetical protein NP_109775.1
hsdS hypothetical protein NP_109777.1
MPN094 hypothetical protein NP_109782.1
MPN095 hypothetical protein NP_109783.1
MPN096 hypothetical protein NP_109784.1
MPN101 hypothetical protein NP_109789.1
MPN103 hypothetical protein NP_109791.1
MPN104 hypothetical protein NP_109792.1
pheS phenylalanine--tRNA ligase subunit alpha NP_109793.1
MPN110 hypothetical protein NP_109798.1
MPN112 hypothetical protein NP_109800.1
infC translation initiation factor IF-3 NP_109803.1
MPN126 metallophosphoesterase NP_109814.1
MPN129 hypothetical protein NP_109817.1
MPN130 hypothetical protein NP_109818.1
ugpC ABC transporter ATP-binding protein NP_109822.1
ugpE ABC transporter permease NP_109824.1
MPN138 hypothetical protein NP_109826.1
MPN145 hypothetical protein NP_109833.1
rbfA ribosome-binding factor A NP_109844.1
yaaC bifunctional riboflavin kinase/FMNadenylyltransferase NP_109846.1
MPN160 hypothetical protein NP_109848.1
MPN162 hypothetical protein NP_109850.1
MPN163 hypothetical protein NP_109851.1
rplW 50S ribosomal protein L23 NP_109855.1
rpsC 30S ribosomal protein S3 NP_109859.1
rplP 50S ribosomal protein L16 NP_109860.1
rpmC 50S ribosomal protein L29 NP_109861.1
rplN 50S ribosomal protein L14 NP_109863.1
rplO 50S ribosomal protein L15 NP_109871.1
secY preprotein translocase subunit SecY NP_109872.1
adk adenylate kinase NP_109873.1
rpoA DNA-directed RNA polymerase subunit alpha NP_109879.1
rpsB 30S ribosomal protein S2 NP_109896.1
MPN212 hypothetical protein NP_109900.1
MPN214 hypothetical protein NP_109902.1
oppB oligopeptide ABC transporter permease NP_109903.1
oppD oligopeptide ABC transporter ATP-bindingprotein NP_109905.1
rplK 50S ribosomal protein L11 NP_109907.1
rplA 50S ribosomal protein L1 NP_109908.1
lgt prolipoprotein diacylglyceryl transferase NP_109912.1
ssb single-stranded DNA binding protein NP_109917.1
rplI 50S ribosomal protein L9 NP_109919.1
gatB aspartyl/glutamyl-tRNA amidotransferase subunitB NP_109926.1
MPN239 hypothetical protein NP_109927.1
trxB thioredoxin reductase NP_109928.1
gmk guanylate kinase NP_109934.1
yjeQ ribosome biogenesis GTPase RsgA NP_109937.1
galE UDP-glucose 4-epimerase NP_109945.1
MPN259 ABC transporter permease NP_109947.1
rbsC ABC transporter permease NP_109948.1
MPN264 hypothetical protein NP_109952.1
trpS tryptophan--tRNA ligase NP_109953.1
MPN268 PTS system transporter subunit IIBC NP_109956.1
MPN271 lipoprotein NP_109959.1
hit1 hydrolase NP_109961.1
MPN276 hypothetical protein NP_109964.1
prrB type I restriction-modification systemspecificity protein NP_109973.2
MPN290 hypothetical protein NP_109978.1
yceC RNA pseudouridine synthase NP_109980.1
MPN295 hypothetical protein NP_109983.1
acpS holo-ACP synthase NP_109986.1
pfkA ATP-dependent 6-phosphofructokinase NP_109990.1
arcC carbamate kinase NP_109995.1
mraW rRNA small subunit methyltransferase H NP_110003.1
nrdF ribonucleotide-diphosphate reductase subunitbeta NP_110010.1
nrdI ribonucleotide reductase stimulatory protein NP_110011.1
rplU 50S ribosomal protein L21 NP_110013.1
rpmA 50S ribosomal protein L27 NP_110015.1
MPN329 ferric uptake regulator NP_110017.1
tig trigger factor NP_110019.1
bcrA ABC transporter ATP-binding protein NP_110022.1
MPN337 hypothetical protein NP_110025.1
MPN338 hypothetical protein NP_110026.1
MPN344 hypothetical protein NP_110032.1
MPN348 5-formyltetrahydrofolate cyclo-ligase NP_110036.1
ygiH glycerol-3-phosphate acyltransferase NP_110038.1
MPN351 Putative tRNA methyltransferase MPN_351 NP_110039.1
MPN359 hypothetical protein NP_110047.1
fpg formamidopyrimidine-DNA glycosylase NP_110068.1
MPN387 hypothetical protein NP_110075.1
lplA lipoate--protein ligase NP_110077.1
pdhD pyruvate dehydrogenase complex dihydrolipoamidedehydrogenase NP_110078.1
pdhB pyruvate dehydrogenase complex component E1subunit beta NP_110080.1
greA transcription elongation factor GreA NP_110089.1
proS proline--tRNA ligase NP_110090.1
MPN403 hypothetical protein NP_110091.1
MPN408 lipoprotein NP_110096.1
MPN410 hypothetical protein NP_110098.1
MPN414 hypothetical protein NP_110102.1
P29 ABC transporter ATP-binding protein NP_110104.1
MPN419 Holliday junction resolvase NP_110106.1
glpQ glycerophosphoryl diester phosphodiesterase NP_110108.1
yidA phosphatase NP_110115.1
cbiO cobalt ABC transporter ATP-binding protein NP_110121.1
come3 hypothetical protein NP_110139.1
MPN462 hypothetical protein NP_110150.1
MPN469 hypothetical protein NP_110157.1
degV hypothetical protein NP_110160.1
MPN478 transcriptional regulator NP_110166.1
MPN479 FMN-dependent NADH-azoreductase NP_110167.1
yibD diacylglycerol beta-glycosyltransferase NP_110171.1
MPN484 hypothetical protein NP_110172.1
MPN488 hypothetical protein NP_110176.1
recA recombinase A NP_110178.1
MPN491 hypothetical protein NP_110179.1
yjfU PTS system ascorbate-specific transportersubunit IIA NP_110182.1
araD L-ribulose-5-phosphate 4-epimerase NP_110186.1
MPN501 hypothetical protein NP_110189.1
MPN504 hypothetical protein NP_110192.1
MPN512 hypothetical protein NP_110200.1
MPN513 hypothetical protein NP_110201.1
MPN514 hypothetical protein NP_110202.1
MPN517 hypothetical protein NP_110205.1
ygl3 Putative tRNA (cytidine(34)-2'-O)-methyltransferase NP_110209.1
MPN524 hypothetical protein NP_110212.1
MPN527 hypothetical protein NP_110215.1
ppa inorganic pyrophosphatase NP_110216.1
MPN530 hypothetical protein NP_110219.1
ackA acetate kinase NP_110222.1
MPN534 hypothetical protein NP_110223.1
fmt methionyl-tRNA formyltransferase NP_110232.1
plsX phosphate acyltransferase NP_110235.1
thrSv threonine--tRNA ligase NP_110242.1
MPN554 singe-stranded DNA-binding protein NP_110243.1
argS arginine--tRNA ligase NP_110245.1
gidB rRNA small subunit methyltransferase G NP_110247.1
MPN559 hypothetical protein NP_110248.1
udk uridine kinase NP_110250.1
outB NH(3)-dependent NAD(+) synthetase NP_110251.1
MPN565 hypothetical protein NP_110254.2
glpQ glycerophosphoryl diester phosphodiesterase NP_110255.1
MPN572 cytosol aminopeptidase NP_110261.1
MPN578 hypothetical protein NP_110267.1
MPN579 hypothetical protein NP_110268.1
MPN580 hypothetical protein NP_110269.1
MPN581 hypothetical protein NP_110270.1
MPN584 hypothetical protein NP_110273.1
MPN586 hypothetical protein NP_110275.1
MPN587 lipoprotein NP_110276.1
MPN589 hypothetical protein NP_110278.1
MPN591 hypothetical protein NP_110280.1
MPN593 hypothetical protein NP_110282.1
atpC ATP synthase subunit epsilon NP_110286.1
atpD ATP synthase subunit beta NP_110287.1
atpH ATP synthase subunit delta NP_110290.1
MPN605 hypothetical protein NP_110294.1
eno enolase NP_110295.1
pstB phosphate ABC transporter ATP-binding protein NP_110298.1
MPN613 hypothetical protein NP_110302.1
MPN614 hypothetical protein NP_110303.1
rpsI 30S ribosomal protein S9 NP_110305.1
rplM 50S ribosomal protein L13 NP_110306.1
MPN626 hypothetical protein NP_110315.1
ptsI phosphoenolpyruvate-protein phosphotransferase NP_110316.1
tpiA triosephosphate isomerase NP_110318.1
MPN638 type I restriction-modification systemspecificity protein NP_110327.1
MPN641 lipoprotein NP_110330.1
MPN642 lipoprotein NP_110331.1
MPN644 lipoprotein NP_110333.1
MPN648 hypothetical protein NP_110337.1
mtlA PTS system mannitol-specific transporter subunitIIBC NP_110340.1
MPN657 hypothetical protein NP_110346.1
trmD NP_110348.1
pilB methionine sulfoxide reductase B NP_110351.1
degV hypothetical protein NP_110353.1
MPN666 hypothetical protein NP_110355.1
MPN673 hypothetical protein NP_110362.1
cysA ABC transporter ATP-binding protein NP_110374.1


The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact
Amjed Alsultan amjed.talib {at} qu {dot} edu {dot} iq
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org