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Mycoplasma genitalium genes (E. coli codon optimized)

Mycoplasma genitalium genes (E. coli codon optimized)

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Mycoplasma genitalium has the smallest genome of any known free-living bacteria, but grows extremely slowly (1 month per reculture) and is Biosafety Level 2. This makes studying its genes difficult for most labs, even though it is one of the best examples of minimal genomes in nature. In order to solve this, FreeGenes has synthesized 138 of 514 Mycoplasma genitalium G37 genes, codon optimized for Escherichia coli. This will allow in-vitro or in-vivo studies of Mycoplasma genitalium’s genes in the most widely available and used host organism. Hopefully, by supporting Mycoplasma research, FreeGenes can encourage synthetic biology laboratories to conduct synthetic genome research.

Note: Hypothetical protein MG_R02130 is no longer included in this collection. 

What is it
This is a collection of as many protein coding sequences from Mycoplasma genitalium G37 as FreeGenes could clone in a single round of cloning. They are all standardized for MoClo assembly and codon optimized for Escherichia coli.

What can it be used for
There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Mycoplasma genome.

This product is made available under the unilateral OpenMTA

Shipped as purified and dried down plasmid DNA stained with Cresol Red in two 96-well plates. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation. Some or all of these items are for use only as permitted by a research exemption.


Where I can find more information

138 unique parts

Instructions for Use:

Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.

Plate Layout

PlateSet: M. genitalium Plate 1, M. genitalium Plate 2


Gene Name NCBI ID Freegenes ID
MG_RS00045 deoxyribonuclease WP_009885976.1 BBF10K_001921
MG_RS00050 DNA primase-like protein WP_010869288.1 BBF10K_001922
MG_RS00055 hypothetical protein WP_009885973.1 BBF10K_001923
MG_RS00100 proline iminopeptidase WP_009885920.1 BBF10K_001930
MG_RS00145 DJ-1 family protein WP_009885913.1 BBF10K_001939
MG_RS00180 aspartate--tRNA ligase WP_009885700.1 BBF10K_001946
MG_RS00185 nicotinate phosphoribosyltransferase WP_029329854.1 BBF10K_001947
MG_RS00260 thymidine phosphorylase WP_009885713.1 BBF10K_001962
MG_RS00280 preprotein translocase subunit SecE WP_010869309.1 BBF10K_001966
MG_RS00285 hypothetical protein WP_009885720.1 BBF10K_001968
MG_RS00295 ribonuclease M5 WP_009885722.1 BBF10K_001970
MG_RS00310 glycosyl transferase family A WP_010869310.1 BBF10K_001973
MG_RS00365 hypothetical protein WP_009885732.1 BBF10K_001983
MG_RS00420 hypothetical protein WP_010869319.1 BBF10K_001994
MG_RS00460 50S ribosomal protein L1 WP_010869323.1 BBF10K_002002
MG_RS00500 30S ribosomal protein S6 WP_009885647.1 BBF10K_002010
MG_RS00505 single-stranded DNA-binding protein WP_010869327.1 BBF10K_002011
MG_RS00515 50S ribosomal protein L9 WP_009885649.1 BBF10K_002013
MG_RS00520 replicative DNA helicase WP_010869329.1 BBF10K_002014
MG_RS00575 ribonuclease R WP_009885662.1 BBF10K_002025
MG_RS00580 TIGR00159 family protein WP_009885663.1 BBF10K_002026
MG_RS00595 protein phosphatase WP_010869334.1 BBF10K_002029
MG_RS00610 glucose-6-phosphate isomerase WP_010869336.1 BBF10K_002032
MG_RS00615 ribulose-phosphate 3-epimerase WP_009885669.1 BBF10K_002033
MG_RS00625 CDP-diacylglycerol--glycerol-3-phosphate3-phosphatidyltransferase WP_081440607.1 BBF10K_002035
MG_RS00630 amidohydrolase WP_009885673.1 BBF10K_002036
MG_RS00640 hypothetical protein WP_010869339.1 BBF10K_002038
MG_RS00675 thiol reductase thioredoxin WP_010869343.1 BBF10K_002045
MG_RS00680 Cof-type HAD-IIB family hydrolase WP_010869344.1 BBF10K_002046
MG_RS00685 tryptophan--tRNA ligase WP_009885680.1 BBF10K_002047
MG_RS00715 hypothetical protein WP_014893951.1 BBF10K_002052
MG_RS00725 DUF1600 domain-containing protein WP_009885689.1 BBF10K_002054
MG_RS00730 Uncharacterized protein MG134 WP_010869347.1 BBF10K_002055
MG_RS00760 hypothetical protein WP_041593667.1 BBF10K_002061
MG_RS00795 hypothetical protein WP_081440608.1 BBF10K_002065
MG_RS00820 ribosome-binding factor A WP_009885829.1 BBF10K_002070
MG_RS00845 DUF2130 domain-containing protein WP_010869355.1 BBF10K_002075
MG_RS00855 hypothetical protein WP_010869357.1 BBF10K_002077
MG_RS00865 50S ribosomal protein L3 WP_010869358.1 BBF10K_002079
MG_RS00870 50S ribosomal protein L4 WP_009885836.1 BBF10K_002080
MG_RS00875 50S ribosomal protein L23 WP_009885837.1 BBF10K_002081
MG_RS00890 50S ribosomal protein L22 WP_009885840.1 BBF10K_002084
MG_RS00905 50S ribosomal protein L29 WP_009885843.1 BBF10K_002087
MG_RS00915 50S ribosomal protein L14 WP_009885845.1 BBF10K_002089
MG_RS00940 50S ribosomal protein L6 WP_010869360.1 BBF10K_002094
MG_RS00945 50S ribosomal protein L18 WP_009885853.1 BBF10K_002095
MG_RS00950 30S ribosomal protein S5 WP_009885854.1 BBF10K_002096
MG_RS00955 50S ribosomal protein L15 WP_010869361.1 BBF10K_002097
MG_RS00965 adenylate kinase WP_009885856.1 BBF10K_002099
MG_RS00980 30S ribosomal protein S13 WP_009885860.1 BBF10K_002103
MG_RS00990 DNA-directed RNA polymerase subunit alpha WP_009885862.1 BBF10K_002105
MG_RS00995 50S ribosomal protein L17 WP_010869362.1 BBF10K_002106
MG_RS01025 adenine methylase WP_010869363.1 BBF10K_002112
MG_RS01035 adhesin WP_041593682.1 BBF10K_002114
MG_RS01040 hypothetical protein WP_049759242.1 BBF10K_002115
MG_RS01050 hypothetical protein WP_010869364.1 BBF10K_002117
MG_RS01060 ABC transporter permease WP_009886002.1 BBF10K_002119
MG_RS01090 adhesin WP_041593671.1 BBF10K_002126
MG_RS01140 hypothetical protein WP_010869370.1 BBF10K_002135
MG_RS01175 Uncharacterized protein MG208 WP_009885745.1 BBF10K_002141
MG_RS01180 RNA pseudouridine synthase WP_009885746.1 BBF10K_002142
MG_RS01185 signal peptidase II WP_009885747.1 BBF10K_002143
MG_RS01200 DivIVA domain-containing protein WP_009885750.1 BBF10K_002146
MG_RS01210 1-acyl-sn-glycerol-3-phosphate acyltransferase WP_009885752.1 BBF10K_002148
MG_RS01220 segregation/condensation protein B WP_009885754.1 BBF10K_002150
MG_RS01290 hypothetical protein WP_010869379.1 BBF10K_002156
MG_RS01305 division/cell wall cluster transcriptionalrepressor MraZ WP_010869380.1 BBF10K_002158
MG_RS01310 ribosomal RNA small subunit methyltransferase H WP_009885760.1 BBF10K_002159
MG_RS01355 class Ib ribonucleoside-diphosphate reductaseassembly flavoprotein NrdI WP_010869386.1 BBF10K_002167
MG_RS01365 50S ribosomal protein L21 WP_009885769.1 BBF10K_002169
MG_RS01380 endonuclease WP_009885772.1 BBF10K_002172
MG_RS01385 transcriptional repressor WP_009885773.1 BBF10K_002173
MG_RS01450 RNA polymerase sigma factor SigA WP_009885787.1 BBF10K_002186
MG_RS01465 Uncharacterized tRNA/rRNA methyltransferase MG252 WP_010869393.1 BBF10K_002189
MG_RS01470 cysteine--tRNA ligase WP_014894005.1 BBF10K_002190
MG_RS01525 hypothetical protein WP_041593673.1 BBF10K_002197
MG_RS01530 adhesin WP_041593674.1 BBF10K_002198
MG_RS01570 dephospho-CoA kinase WP_009885895.1 BBF10K_002205
MG_RS01585 hypothetical protein WP_009885898.1 BBF10K_002208
MG_RS01600 hypothetical protein WP_009885901.1 BBF10K_002211
MG_RS01625 pyruvate dehydrogenase E1 component subunitalpha WP_009885906.1 BBF10K_002216
MG_RS01640 hypothetical protein WP_010869403.1 BBF10K_002219
MG_RS01645 Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase WP_009885909.1 BBF10K_002220
MG_RS01650 hypothetical protein WP_010869404.1 BBF10K_002221
MG_RS01720 hypothetical protein WP_041593676.1 BBF10K_002230
MG_RS01745 high affinity transport system protein p37 WP_009885873.1 BBF10K_002234
MG_RS01760 Holliday junction resolvase RuvX WP_010869411.1 BBF10K_002237
MG_RS01775 MFS transporter WP_014894023.1 BBF10K_002240
MG_RS01805 phosphoglycerate kinase WP_010869417.1 BBF10K_002246
MG_RS01860 30S ribosomal protein S4 WP_009885889.1 BBF10K_002257
MG_RS01880 hypothetical protein WP_009885989.1 BBF10K_002261
MG_RS01885 hypothetical protein WP_009885990.1 BBF10K_002262
MG_RS01890 Cytadherence high molecular weight protein 3 WP_010869426.1 BBF10K_002263
MG_RS01970 TrkA family potassium uptake protein WP_010869432.1 BBF10K_002270
MG_RS01975 hypothetical protein WP_014894034.1 BBF10K_002271
MG_RS02000 hypothetical protein WP_010869434.1 BBF10K_002276
MG_RS02045 diacylglycerol beta-glycosyltransferase WP_010869439.1 BBF10K_002285
MG_RS02055 iron-sulfur cluster scaffold-like protein WP_009885968.1 BBF10K_002287
MG_RS02065 protein RecA WP_009885970.1 BBF10K_002289
MG_RS02070 hypothetical protein WP_041593679.1 BBF10K_002290
MG_RS02840 adhesin WP_081440609.1 BBF10K_002291
MG_RS02080 adhesin WP_041593680.1 BBF10K_002292
MG_RS02120 tRNA (guanine-N(7)-)-methyltransferase WP_009885805.1 BBF10K_002300
MG_RS02140 hypothetical protein WP_009885807.1 BBF10K_002302
MG_RS02145 hypothetical protein WP_010869445.1 BBF10K_002303
MG_RS02160 inorganic pyrophosphatase WP_009885809.1 BBF10K_002305
MG_RS02165 Holliday junction DNA helicase RecU WP_009885810.1 BBF10K_002306
MG_RS02175 DUF1951 domain-containing protein WP_009885812.1 BBF10K_002308
MG_RS02180 chaperone protein ClpB WP_010869446.1 BBF10K_002309
MG_RS02190 acetate kinase WP_009885814.1 BBF10K_002311
MG_RS02215 DNA polymerase IV WP_010869448.1 BBF10K_002316
MG_RS02220 50S ribosomal protein L10 WP_009885818.1 BBF10K_002317
MG_RS02260 DAK2 domain-containing protein WP_010869453.1 BBF10K_002326
MG_RS02265 RluA family pseudouridine synthase WP_014894681.1 BBF10K_002327
MG_RS02270 bifunctional oligoribonuclease/PAP phosphataseNrnA WP_010869455.1 BBF10K_002328
MG_RS02310 tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG WP_010869458.1 BBF10K_002336
MG_RS02320 hypothetical protein WP_010869459.1 BBF10K_002338
MG_RS02350 hypothetical protein WP_009885949.1 BBF10K_002343
MG_RS02370 hypothetical protein WP_009885633.1 BBF10K_002347
MG_RS02390 co-chaperone GroES WP_010869464.1 BBF10K_002351
MG_RS02410 ribose 5-phosphate isomerase B WP_009885626.1 BBF10K_002355
MG_RS02445 ATP synthase subunit B WP_009885620.1 BBF10K_002362
MG_RS02450 ATP synthase subunit C WP_009885619.1 BBF10K_002363
MG_RS02470 Peptide methionine sulfoxide reductase MsrA WP_010869470.1 BBF10K_002367
MG_RS02525 hypothetical protein WP_010869478.1 BBF10K_002378
MG_RS02550 sigma-70 family RNA polymerase sigma factor WP_009885604.1 BBF10K_002384
MG_RS02580 UMP kinase WP_009885599.1 BBF10K_002390
MG_RS02600 hypothetical protein WP_009885595.1 BBF10K_002394
MG_RS02610 hypothetical protein WP_009885592.1 BBF10K_002396
MG_RS02630 tRNA (guanine-N(1)-)-methyltransferase WP_010869485.1 BBF10K_002400
MG_RS02645 Peptide methionine sulfoxide reductase MsrB WP_009885587.1 BBF10K_002403
MG_RS02675 organic hydroperoxide reductase OsmC/OhrA WP_041361276.1 BBF10K_002408
MG_RS02685 hypothetical protein WP_009885577.1 BBF10K_002410
MG_RS02700 hypoxanthine-guanine phosphoribosyltransferase WP_009885573.1 BBF10K_002413
MG_RS02705 hypothetical protein WP_009885572.1 BBF10K_002414
MG_RS02720 glutamate--tRNA ligase WP_009885570.1 BBF10K_002417
MG_RS02730 protein translocase component YidC WP_009885569.1 BBF10K_002419
MG_RS02765 hypothetical protein WP_009885564.1 BBF10K_002426

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The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact Country
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org United States
Ery Fukushima ery.fukushima {at} ikiam {dot} edu {dot} ec Ecuador
Majed Alghamdi mafa2 {at} le {dot} ac {dot} uk United Kingdom
Amjed Alsultan amjed.talib {at} qu {dot} edu {dot} iq Iraq

Download all of this information as a CSV from our GitHub.