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JCVI-Syn3.0 genes (E. coli codon optimized)

JCVI-Syn3.0 genes (E. coli codon optimized)

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Do you want to study and experiment with minimal genomes? Determine the purpose of unknown genes of essential function? Then look no further!


JCVI-Syn3.0 genes (E. coli codon optimized) is a collection of 395 essential MoClo-compatible genes from synthetic bacterium JCVI-Syn3A, recoded for expression in E. coli. With this collection, you can attempt to to recreate essential gene pathways in-vitro, purify the proteins for functional studies, or create a synthetic MoClo-compatible Mycoplasma genome.

What is JCVI-Syn3A?

For nearly 20 years, the J. Craig Venter Institute (JCVI) worked on creating a minimal synthetic bacterial genome, which they finally achieved in 2016. They named the novel synthetic organism JCVI-Syn3.0. JCVI-Syn3A, which is JCVI-Syn3.0 with 16 additional genes, is more commonly used because it has a more stable phenotype[1]. All 493 genes in JCVI-Syn3.0 are essential for growth and 149 of those genes' functions are unknown.

Collection Design

Recoding was performed using scripts that stochastically assigned codons to amino acids. The codon table used was the FreqB table from "Design Parameters to Control Synthetic Gene Expression in Escherichia coli", which was used because it was shown to express proteins well and was recommended to us by JCVI. All genes where inserted into the BbsI:BbsI cloning sites of pOpen_build, which is individually available through our website.
14 of the 510 JCVI-Syn3A genes included in this distribution do not exist in JCVI-Syn3.0. The additional 14 genes can be found in excel spreadsheet "Genes added to JCVI-syn3.0 to make JCVI-syn3A.xlsx", which was kindly provided by John Glass at JCVI.


[1] John Glass, one of the leaders of the minimal genome project, noted: "Note that 2 of the genes we added to JCVI-syn3.0 to make JCVI-syn3A, which has a more stable phenotype, are pseudogenes. I see no reason for you to remake those genes since they can just be PCR amplified from genomic DNA... Note that one of those pseudogenes appears to actually be synthesized. JCVISYN3A_0602, which is a pseudogene. We know that if this gene is removed from JCVI-syn3A we get the giant cell phenotype rather than the more normal JCVI-syn3A phenotype. We know because of multiple genome sequences, that there is a frameshift in the gene relative to the same gene in other Mycoplasma mycoides strains. Still, it could be that there is some RNA editing to produce the protein. Thus, I would like you to make the protein that we think JCVISYN3A_0602 was supposed to encode."

This product is made available under the unilateral OpenMTA.

Some or all of these items are for use only as permitted by a research exemption.

Shipped as purified and dried down plasmid DNA stained with Cresol Red in five 96-well plates. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.  Plate Layouts


Prior Kit Versions

If your plates were created before July 26 2021, plate layouts can be found here.

Instructions for Use:

Culture at 37C overnight, with shaking at around 300rpm. The selection marker for all parts is ampicillin.


Gene Name NCBI ID Freegenes ID
MMSYN1_0001 Chromosomal replication initiator protein DnaA AMW76285.1 BBF10K_000001
MMSYN1_0002 AMW76286.1 BBF10K_000002
MMSYN1_0003 Ribonuclease M5 AMW76287.1 BBF10K_000003
MMSYN1_0005 Uncharacterized protein AMW76289.1 BBF10K_000005
MMSYN1_0007 AMW76291.1 BBF10K_000007
MMSYN1_0008 ABC transporter permease AMW76292.1 BBF10K_000008
MMSYN1_0009 AMW76293.1 BBF10K_000009
MMSYN1_0010 AMW76294.1 BBF10K_000010
MMSYN1_0011 AMW76295.1 BBF10K_000011
MMSYN1_0012 AMW76296.1 BBF10K_000012
MMSYN1_0029 FMN-dependent NADH-azoreductase AMW76300.1 BBF10K_000015
MMSYN1_0033 AMW76302.1 BBF10K_000017
MMSYN1_0034 AMW76303.1 BBF10K_000018
MMSYN1_0039 AMW76304.1 BBF10K_000019
MMSYN1_0040 AMW76305.1 BBF10K_000020
MMSYN1_0042 AMW76306.1 BBF10K_000021
MMSYN1_0043 tRNA1(Val) (Adenine(37)-N6)-methyltransferase AMW76307.1 BBF10K_000022
MMSYN1_0044 DNA polymerase III delta subunit AMW76308.1 BBF10K_000023
MMSYN1_0045 AMW76309.1 BBF10K_000024
MMSYN1_0046 Recombination protein RecR AMW76310.1 BBF10K_000025
MMSYN1_0047 DNA polymerase III subunit gamma/tau AMW76311.1 BBF10K_000026
MMSYN1_0054 Peroxiredoxin AMW76312.1 BBF10K_000027
MMSYN1_0060 AMW76313.1 BBF10K_000028
MMSYN1_0061 Serine--tRNA ligase AMW76314.1 BBF10K_000029
MMSYN1_0063 tRNA-dihydrouridine synthase AMW76315.1 BBF10K_000030
MMSYN1_0065 Thioredoxin AMW76317.1 BBF10K_000032
MMSYN1_0066 AMW76318.1 BBF10K_000033
MMSYN1_0077 Cof-type HAD-IIB family hydrolase AMW76320.1 BBF10K_000035
MMSYN1_0079 tRNA N6-adenosine threonylcarbamoyltransferase AMW76321.1 BBF10K_000036
MMSYN1_0094 Putative membrane protein AMW76325.1 BBF10K_000038
MMSYN1_0097 ~5'-3' exonuclease AMW76327.1 BBF10K_000040
MMSYN1_0106 AMW76329.1 BBF10K_000041
MMSYN1_0107 Transcription antitermination protein NusB AMW76330.1 BBF10K_000042
MMSYN1_0108 AMW76331.1 BBF10K_000043
MMSYN1_0109 Probable endonuclease 4 AMW76332.1 BBF10K_000044
MMSYN1_0113 AMW76333.1 BBF10K_000045
MMSYN1_0115 AMW76335.1 BBF10K_000047
MMSYN1_0116 Uncharacterized protein AMW76336.1 BBF10K_000048
MMSYN1_0117 Glycerol-3-phosphate acyltransferase AMW76337.1 BBF10K_000049
MMSYN1_0126 Glutamate--tRNA ligase AMW76338.1 BBF10K_000050
MMSYN1_0127 HD domain-containing protein AMW76339.1 BBF10K_000051
MMSYN1_0128 DNA-directed RNA polymerase subunit delta AMW76340.1 BBF10K_000052
MMSYN1_0129 AMW76341.1 BBF10K_000053
MMSYN1_0131 Fructose-1 AMW76342.1 BBF10K_000054
MMSYN1_0132 AMW76343.1 BBF10K_000055
MMSYN1_0133 Peptidase_S8 domain-containing protein AMW76344.1 BBF10K_000056
MMSYN1_0138 Uncharacterized protein AMW76346.1 BBF10K_000057
MMSYN1_0139 Bifunctional oligoribonuclease/PAP phosphatase NrnA AMW76347.1 BBF10K_000058
MMSYN1_0141 Peptide chain release factor 1 AMW76349.1 BBF10K_000060
MMSYN1_0142 Release factor glutamine methyltransferase AMW76350.1 BBF10K_000061
MMSYN1_0143 AMW76351.1 BBF10K_000062
MMSYN1_0144 YrdC-like domain-containing protein AMW76352.1 BBF10K_000063
MMSYN1_0145 AMW76353.1 BBF10K_000064
MMSYN1_0146 AMW76354.1 BBF10K_000065
MMSYN1_0147 AMW76355.1 BBF10K_000066
MMSYN1_0148 30S ribosomal protein S12 AMW76356.1 BBF10K_000067
MMSYN1_0150 Elongation factor G AMW76358.1 BBF10K_000069
MMSYN1_0151 Elongation factor Tu AMW76359.1 BBF10K_000070
MMSYN1_0154 Leucyl aminopeptidase AMW76531.1 BBF10K_000071
MMSYN1_0163 AMW76360.1 BBF10K_000072
MMSYN1_0164 AMW76361.1 BBF10K_000073
MMSYN1_0165 ABC transporter permease AMW76362.1 BBF10K_000074
MMSYN1_0166 ABC transporter permease AMW76363.1 BBF10K_000075
MMSYN1_0167 Peptide ABC transporter ATP-binding protein AMW76364.1 BBF10K_000076
MMSYN1_0168 AMW76365.1 BBF10K_000077
MMSYN1_0196 AMW76368.1 BBF10K_000080
MMSYN1_0197 Spermidine/putrescine import ATP-binding protein PotA AMW76369.1 BBF10K_000081
MMSYN1_0198 50S ribosomal protein L20 AMW76370.1 BBF10K_000082
MMSYN1_0200 Translation initiation factor IF-3 AMW76372.1 BBF10K_000083
MMSYN1_0202 ~16S rRNA (Guanine(966)-N(2))-methyltransferase RsmD AMW76374.1 BBF10K_000085
MMSYN1_0203 Guanylate kinase AMW76375.1 BBF10K_000086
MMSYN1_0213 BBF10K_000087
MMSYN1_0214 AMW76377.1 BBF10K_000088
MMSYN1_0215 Putative pre-16S rRNA nuclease AMW76378.1 BBF10K_000089
MMSYN1_0216 AMW76379.1 BBF10K_000090
MMSYN1_0218 AMW76380.1 BBF10K_000091
MMSYN1_0220 ATP-dependent 6-phosphofructokinase AMW76381.1 BBF10K_000092
MMSYN1_0221 Pyruvate kinase AMW76382.1 BBF10K_000093
MMSYN1_0227 AMW76384.1 BBF10K_000095
MMSYN1_0228 AMW76385.1 BBF10K_000096
MMSYN1_0229 Phosphate acetyltransferase AMW76386.1 BBF10K_000097
MMSYN1_0230 AMW76387.1 BBF10K_000098
MMSYN1_0233 Phosphoenolpyruvate-protein phosphotransferase AMW76388.1 BBF10K_000099
MMSYN1_0234 PTS glucose transporter subunit IIA AMW76389.1 BBF10K_000100
MMSYN1_0238 30S ribosomal protein S4 AMW76391.1 BBF10K_000101
MMSYN1_0239 Uncharacterized protein AMW76392.1 BBF10K_000102
MMSYN1_0240 AMW76393.1 BBF10K_000103
MMSYN1_0247 Probable GTP-binding protein EngB AMW76394.1 BBF10K_000104
MMSYN1_0248 Uncharacterized protein AMW76395.1 BBF10K_000105
MMSYN1_0253 Transcription elongation factor GreA AMW76398.1 BBF10K_000108
MMSYN1_0254 AMW76399.1 BBF10K_000109
MMSYN1_0257 AMW76400.1 BBF10K_000110
MMSYN1_0259 AMW76401.1 BBF10K_000111
MMSYN1_0260 Valine--tRNA ligase AMW76402.1 BBF10K_000112
MMSYN1_0262 Ribulose-phosphate 3-epimerase AMW76403.1 BBF10K_000113
MMSYN1_0263 Small ribosomal subunit biogenesis GTPase RsgA AMW76404.1 BBF10K_000114
MMSYN1_0264 Serine/threonine protein kinase AMW76405.1 BBF10K_000115
MMSYN1_0270 tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE AMW76406.1 BBF10K_000116
MMSYN1_0271 tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB AMW76407.1 BBF10K_000117
MMSYN1_0281 Uncharacterized protein AMW76408.1 BBF10K_000118
MMSYN1_0282 AMW76409.1 BBF10K_000119
MMSYN1_0283 Ribonuclease H AMW76410.1 BBF10K_000120
MMSYN1_0285 Elongation factor 4 AMW76411.1 BBF10K_000121
MMSYN1_0286 Uncharacterized protein AMW76412.1 BBF10K_000122
MMSYN1_0287 AMW76413.1 BBF10K_000123
MMSYN1_0288 Histidine--tRNA ligase AMW76414.1 BBF10K_000124
MMSYN1_0289 Ribosome-binding factor A AMW76415.1 BBF10K_000125
MMSYN1_0290 AMW76416.1 BBF10K_000126
MMSYN1_0291 FAD synthetase AMW76417.1 BBF10K_000127
MMSYN1_0296 Uncharacterized protein AMW76419.1 BBF10K_000128
MMSYN1_0298 ~50S ribosomal protein L7ae AMW76421.1 BBF10K_000130
MMSYN1_0300 AMW76423.1 BBF10K_000131
MMSYN1_0301 AMW76424.1 BBF10K_000132
MMSYN1_0302 AMW76425.1 BBF10K_000133
MMSYN1_0304 Phosphatidate cytidylyltransferase AMW76427.1 BBF10K_000135
MMSYN1_0305 Aminopeptidase P family protein AMW76428.1 BBF10K_000136
MMSYN1_0308 AMW76429.1 BBF10K_000137
MMSYN1_0314 Folate family ECF transporter S component AMW76430.1 BBF10K_000138
MMSYN1_0316 AMW76432.1 BBF10K_000139
MMSYN1_0325 AMW76434.1 BBF10K_000140
MMSYN1_0326 Uncharacterized protein AMW76435.1 BBF10K_000141
MMSYN1_0327 AMW76436.1 BBF10K_000142
MMSYN1_0328 AMW76437.1 BBF10K_000143
MMSYN1_0329 rRNA pseudouridine synthase AMW76438.1 BBF10K_000144
MMSYN1_0330 Deoxynucleoside kinase AMW76439.1 BBF10K_000145
MMSYN1_0332 AMW76440.1 BBF10K_000146
MMSYN1_0338 Lipoprotein AMW76441.1 BBF10K_000147
MMSYN1_0344 Inorganic pyrophosphatase AMW76442.1 BBF10K_000148
MMSYN1_0345 ECF transporter S component AMW76443.1 BBF10K_000149
MMSYN1_0346 Uncharacterized protein AMW76444.1 BBF10K_000150
MMSYN1_0352 DnaD family protein AMW76448.1 BBF10K_000153
MMSYN1_0353 DivIVA domain-containing protein AMW76449.1 BBF10K_000154
MMSYN1_0359 AMW76450.1 BBF10K_000155
MMSYN1_0360 AMW76451.1 BBF10K_000156
MMSYN1_0361 Ribosomal RNA large subunit methyltransferase H AMW76452.1 BBF10K_000157
MMSYN1_0363 Ribosome maturation factor RimM AMW76454.1 BBF10K_000158
MMSYN1_0364 AMW76455.1 BBF10K_000159
MMSYN1_0365 50S ribosomal protein L19 AMW76456.1 BBF10K_000160
MMSYN1_0366 Ribosome biogenesis GTPase A AMW76457.1 BBF10K_000161
MMSYN1_0371 AMW76458.1 BBF10K_000162
MMSYN1_0373 AMW76460.1 BBF10K_000164
MMSYN1_0375 AMW76461.1 BBF10K_000165
MMSYN1_0376 AMW76462.1 BBF10K_000166
MMSYN1_0378 AMW76464.1 BBF10K_000168
MMSYN1_0380 AMW76466.1 BBF10K_000169
MMSYN1_0381 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase AMW76467.1 BBF10K_000170
MMSYN1_0387 AMW76469.1 BBF10K_000172
MMSYN1_0388 Uncharacterized protein AMW76470.1 BBF10K_000173
MMSYN1_0389 Uncharacterized protein AMW76471.1 BBF10K_000174
MMSYN1_0390 Methionyl-tRNA formyltransferase AMW76472.1 BBF10K_000175
MMSYN1_0391 Elongation factor P AMW76473.1 BBF10K_000176
MMSYN1_0392 Uncharacterized protein AMW76474.1 BBF10K_000177
MMSYN1_0394 AMW76475.1 BBF10K_000178
MMSYN1_0398 MOLPALP family lipoprotein BBF10K_000179
MMSYN1_0401 AMW76479.1 BBF10K_000182
MMSYN1_0402 Endoribonuclease YbeY AMW76480.1 BBF10K_000183
MMSYN1_0403 AMW76481.1 BBF10K_000184
MMSYN1_0404 DNA repair protein RecO AMW76482.1 BBF10K_000185
MMSYN1_0405 AMW76483.1 BBF10K_000186
MMSYN1_0406 AMW76484.1 BBF10K_000187
MMSYN1_0407 RNA polymerase sigma factor AMW76485.1 BBF10K_000188
MMSYN1_0408 AMW76486.1 BBF10K_000189
MMSYN1_0409 AMW76487.1 BBF10K_000190
MMSYN1_0410 ATP-dependent helicase AMW76488.1 BBF10K_000191
MMSYN1_0411 AMW76489.1 BBF10K_000192
MMSYN1_0412 Putative membrane protein AMW76490.1 BBF10K_000193
MMSYN1_0413 Adenine phosphoribosyltransferase AMW76491.1 BBF10K_000194
MMSYN1_0416 Uncharacterized protein AMW76494.1 BBF10K_000197
MMSYN1_0418 Ribonuclease 3 AMW76495.1 BBF10K_000198
MMSYN1_0420 AMW76497.1 BBF10K_000200
MMSYN1_0421 AMW76498.1 BBF10K_000201
MMSYN1_0424 Uncharacterized protein AMW76500.1 BBF10K_000202
MMSYN1_0425 AMW76501.1 BBF10K_000203
MMSYN1_0426 Phosphate ABC transporter permease PtsA AMW76502.1 BBF10K_000204
MMSYN1_0427 Phosphate import ATP-binding protein PstB AMW76503.1 BBF10K_000205
MMSYN1_0428 AMW76504.1 BBF10K_000206
MMSYN1_0430 AMW76506.1 BBF10K_000208
MMSYN1_0431 TIGR00282 family metallophosphoesterase AMW76507.1 BBF10K_000209
MMSYN1_0434 Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO AMW76510.1 BBF10K_000212
MMSYN1_0435 Phosphomannose isomerase type I AMW76511.1 BBF10K_000213
MMSYN1_0437 HD domain-containing protein AMW76512.1 BBF10K_000214
MMSYN1_0438 AMW76513.1 BBF10K_000215
MMSYN1_0439 AMW76514.1 BBF10K_000216
MMSYN1_0441 AMW76516.1 BBF10K_000218
MMSYN1_0442 Iron-sulfur cluster assembly scaffold protein AMW76517.1 BBF10K_000219
MMSYN1_0443 5-formyltetrahydrofolate cyclo-ligase AMW76518.1 BBF10K_000220
MMSYN1_0444 AMW76519.1 BBF10K_000221
MMSYN1_0445 AMW76520.1 BBF10K_000222
MMSYN1_0447 dUTPase superfamily protein AMW76521.1 BBF10K_000223
MMSYN1_0448 AMW76522.1 BBF10K_000224
MMSYN1_0451 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase AMW76523.1 BBF10K_000225
MMSYN1_0452 AMW76524.1 BBF10K_000226
MMSYN1_0475 AMW76526.1 BBF10K_000228
MMSYN1_0478 Uncharacterized protein AMW76527.1 BBF10K_000229
MMSYN1_0479 Uncharacterized protein AMW76528.1 BBF10K_000230
MMSYN1_0481 Lipoprotein (VlcE) AMW76529.1 BBF10K_000231
MMSYN1_0493 Xaa-His dipeptidase AMW76532.1 BBF10K_000232
MMSYN1_0494 Putative N-acetylmannosamine-6-phosphate 2-epimerase AMW76533.1 BBF10K_000233
MMSYN1_0495 ROK family protein AMW76534.1 BBF10K_000234
MMSYN1_0501 50S ribosomal protein L21 AMW76537.1 BBF10K_000235
MMSYN1_0503 Uncharacterized protein AMW76538.1 BBF10K_000236
MMSYN1_0504 Ribosomal RNA small subunit methyltransferase I AMW76539.1 BBF10K_000237
MMSYN1_0511 Uncharacterized protein AMW76541.1 BBF10K_000239
MMSYN1_0512 AMW76542.1 BBF10K_000240
MMSYN1_0513 Holo-[acyl-carrier-protein] synthase AMW76543.1 BBF10K_000241
MMSYN1_0515 dCMP deaminase AMW76544.1 BBF10K_000242
MMSYN1_0517 AMW76546.1 BBF10K_000244
MMSYN1_0518 AMW76547.1 BBF10K_000245
MMSYN1_0523 Uncharacterized protein AMW76549.1 BBF10K_000247
MMSYN1_0524 Ribosomal RNA small subunit methyltransferase H AMW76550.1 BBF10K_000248
MMSYN1_0525 Transcriptional regulator MraZ AMW76551.1 BBF10K_000249
MMSYN1_0528 Phenylalanine--tRNA ligase beta subunit AMW76553.1 BBF10K_000250
MMSYN1_0529 Phenylalanine--tRNA ligase alpha subunit AMW76554.1 BBF10K_000251
MMSYN1_0530 Uncharacterized protein AMW76555.1 BBF10K_000252
MMSYN1_0531 ABC transporter permease BBF10K_000253
MMSYN1_0535 AMW76557.1 BBF10K_000254
MMSYN1_0536 Ribosome-recycling factor AMW76558.1 BBF10K_000255
MMSYN1_0539 AMW76560.1 BBF10K_000257
MMSYN1_0540 30S ribosomal protein S2 AMW76561.1 BBF10K_000258
MMSYN1_0541 AMW76562.1 BBF10K_000259
MMSYN1_0542 AMW76563.1 BBF10K_000260
MMSYN1_0543 Protein GrpE AMW76564.1 BBF10K_000261
MMSYN1_0544 Heat-inducible transcription repressor HrcA AMW76565.1 BBF10K_000262
MMSYN1_0545 AMW76566.1 BBF10K_000263
MMSYN1_0601 AMW76569.1 BBF10K_000265
MMSYN1_0606 AMW76570.1 BBF10K_000266
MMSYN1_0607 Glyceraldehyde-3-phosphate dehydrogenase AMW76571.1 BBF10K_000267
MMSYN1_0608 AMW76572.1 BBF10K_000268
MMSYN1_0609 Chromosome replication initiation/membrane attachment protein AMW76573.1 BBF10K_000269
MMSYN1_0611 AMW76574.1 BBF10K_000270
MMSYN1_0612 AMW76575.1 BBF10K_000271
MMSYN1_0613 Tyrosine--tRNA ligase AMW76576.1 BBF10K_000272
MMSYN1_0614 AMW76577.1 BBF10K_000273
MMSYN1_0615 Phenylalanine--tRNA ligase beta subunit AMW76578.1 BBF10K_000274
MMSYN1_0616 AMW76579.1 BBF10K_000275
MMSYN1_0617 DegV family protein AMW76580.1 BBF10K_000276
MMSYN1_0620 Transcriptional repressor AMW76581.1 BBF10K_000277
MMSYN1_0632 AMW76583.1 BBF10K_000278
MMSYN1_0634 Leucine--tRNA ligase AMW76584.1 BBF10K_000279
MMSYN1_0636 AMW76585.1 BBF10K_000280
MMSYN1_0637 30S ribosomal protein S9 AMW76586.1 BBF10K_000281
MMSYN1_0638 AMW76587.1 BBF10K_000282
MMSYN1_0639 AMW76588.1 BBF10K_000283
MMSYN1_0640 tRNA pseudouridine synthase A AMW76589.1 BBF10K_000284
MMSYN1_0641 Energy-coupling factor transporter transmembrane protein EcfT AMW76590.1 BBF10K_000285
MMSYN1_0642 Energy-coupling factor transporter ATP-binding protein EcfA AMW76591.1 BBF10K_000286
MMSYN1_0643 AMW76592.1 BBF10K_000287
MMSYN1_0644 AMW76593.1 BBF10K_000288
MMSYN1_0645 DNA-directed RNA polymerase subunit alpha AMW76594.1 BBF10K_000289
MMSYN1_0646 30S ribosomal protein S11 AMW76595.1 BBF10K_000290
MMSYN1_0647 30S ribosomal protein S13 AMW76596.1 BBF10K_000291
MMSYN1_0650 AMW76599.1 BBF10K_000292
MMSYN1_0651 Adenylate kinase BBF10K_000293
MMSYN1_0652 Protein translocase subunit SecY AMW76601.1 BBF10K_000294
MMSYN1_0653 50S ribosomal protein L15 AMW76602.1 BBF10K_000295
MMSYN1_0654 30S ribosomal protein S5 AMW76603.1 BBF10K_000296
MMSYN1_0655 AMW76604.1 BBF10K_000297
MMSYN1_0656 50S ribosomal protein L6 AMW76605.1 BBF10K_000298
MMSYN1_0657 30S ribosomal protein S8 AMW76606.1 BBF10K_000299
MMSYN1_0659 50S ribosomal protein L5 AMW76608.1 BBF10K_000300
MMSYN1_0660 50S ribosomal protein L24 AMW76609.1 BBF10K_000301
MMSYN1_0661 50S ribosomal protein L14 AMW76610.1 BBF10K_000302
MMSYN1_0663 50S ribosomal protein L29 AMW76612.1 BBF10K_000303
MMSYN1_0664 50S ribosomal protein L16 AMW76613.1 BBF10K_000304
MMSYN1_0665 30S ribosomal protein S3 AMW76614.1 BBF10K_000305
MMSYN1_0666 50S ribosomal protein L22 AMW76615.1 BBF10K_000306
MMSYN1_0668 50S ribosomal protein L2 AMW76617.1 BBF10K_000307
MMSYN1_0670 50S ribosomal protein L4 AMW76619.1 BBF10K_000308
MMSYN1_0671 50S ribosomal protein L3 AMW76620.1 BBF10K_000309
MMSYN1_0672 30S ribosomal protein S10 AMW76621.1 BBF10K_000310
MMSYN1_0684 Bifunctional protein FolD AMW76622.1 BBF10K_000311
MMSYN1_0685 Sodium transporter AMW76623.1 BBF10K_000312
MMSYN1_0686 TrkA family potassium uptake protein AMW76624.1 BBF10K_000313
MMSYN1_0687 AMW76625.1 BBF10K_000314
MMSYN1_0688 AMW76626.1 BBF10K_000315
MMSYN1_0690 DNA ligase AMW76628.1 BBF10K_000316
MMSYN1_0691 AMW76629.1 BBF10K_000317
MMSYN1_0692 RluA family pseudouridine synthase AMW76630.1 BBF10K_000318
MMSYN1_0693 AMW76631.1 BBF10K_000319
MMSYN1_0695 AMW76633.1 BBF10K_000320
MMSYN1_0696 RDD family protein AMW76634.1 BBF10K_000321
MMSYN1_0697 Glycosyltransferase family 2 protein AMW76635.1 BBF10K_000322
MMSYN1_0706 AMW76636.1 BBF10K_000323
MMSYN1_0707 Alkylphosphonate ABC transporter ATP-binding protein AMW76637.1 BBF10K_000324
MMSYN1_0708 AMW76638.1 BBF10K_000325
MMSYN1_0710 Cof-type HAD-IIB family hydrolase AMW76639.1 BBF10K_000326
MMSYN1_0727 AMW76641.1 BBF10K_000328
MMSYN1_0729 2,3-bisphosphoglycerate-independent phosphoglycerate mutase AMW76643.1 BBF10K_000330
MMSYN1_0730 Uncharacterized protein AMW76644.1 BBF10K_000331
MMSYN1_0732 Deoxyribose-phosphate aldolase AMW76645.1 BBF10K_000332
MMSYN1_0733 Phospho-sugar mutase AMW76646.1 BBF10K_000333
MMSYN1_0747 Purine-nucleoside phosphorylase AMW76647.1 BBF10K_000334
MMSYN1_0772 Protein NrdI AMW76649.1 BBF10K_000336
MMSYN1_0773 Class 1b ribonucleoside-diphosphate reductase subunit beta AMW76650.1 BBF10K_000337
MMSYN1_0775 Ribonuclease R AMW76652.1 BBF10K_000338
MMSYN1_0776 SsrA-binding protein AMW76653.1 BBF10K_000339
MMSYN1_0777 Uncharacterized protein AMW76654.1 BBF10K_000340
MMSYN1_0779 AMW76656.1 BBF10K_000341
MMSYN1_0787 Magnesium-translocating P-type ATPase AMW76657.1 BBF10K_000342
MMSYN1_0789 ATP-synt_DE_N domain-containing protein AMW76658.1 BBF10K_000343
MMSYN1_0790 AMW76659.1 BBF10K_000344
MMSYN1_0791 ATP synthase gamma chain AMW76660.1 BBF10K_000345
MMSYN1_0792 ATP synthase subunit alpha AMW76661.1 BBF10K_000346
MMSYN1_0793 ATP synthase subunit delta AMW76662.1 BBF10K_000347
MMSYN1_0794 ATP synthase subunit b AMW76663.1 BBF10K_000348
MMSYN1_0795 ATP synthase subunit c AMW76664.1 BBF10K_000349
MMSYN1_0796 F0F1 ATP synthase subunit A AMW76665.1 BBF10K_000350
MMSYN1_0798 Uracil phosphoribosyltransferase AMW76667.1 BBF10K_000351
MMSYN1_0799 Serine hydroxymethyltransferase AMW76668.1 BBF10K_000352
MMSYN1_0800 Ribose 5-phosphate isomerase B AMW76669.1 BBF10K_000353
MMSYN1_0803 AMW76670.1 BBF10K_000354
MMSYN1_0805 Uncharacterized protein AMW76672.1 BBF10K_000356
MMSYN1_0807 50S ribosomal protein L10 AMW76674.1 BBF10K_000358
MMSYN1_0809 AMW76675.1 BBF10K_000359
MMSYN1_0810 50S ribosomal protein L11 AMW76676.1 BBF10K_000360
MMSYN1_0813 AMW76677.1 BBF10K_000361
MMSYN1_0814 AMW76678.1 BBF10K_000362
MMSYN1_0817 Probable cell division protein WhiA AMW76679.1 BBF10K_000363
MMSYN1_0818 Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase AMW76680.1 BBF10K_000364
MMSYN1_0819 Thioredoxin reductase AMW76681.1 BBF10K_000365
MMSYN1_0821 HPr kinase/phosphorylase AMW76683.1 BBF10K_000367
MMSYN1_0822 Folate family ECF transporter S component AMW76684.1 BBF10K_000368
MMSYN1_0823 Folate synthetase-polyglutamyl folate synthetase AMW76685.1 BBF10K_000369
MMSYN1_0824 AMW76686.1 BBF10K_000370
MMSYN1_0825 AMW76687.1 BBF10K_000371
MMSYN1_0826 DNA polymerase III subunit delta AMW76688.1 BBF10K_000372
MMSYN1_0827 AMW76689.1 BBF10K_000373
MMSYN1_0831 Ribose-phosphate pyrophosphokinase AMW76691.1 BBF10K_000374
MMSYN1_0832 Peptidyl-tRNA hydrolase AMW76692.1 BBF10K_000375
MMSYN1_0833 50S ribosomal protein L9 AMW76693.1 BBF10K_000376
MMSYN1_0834 Replicative DNA helicase AMW76694.1 BBF10K_000377
MMSYN1_0835 Uncharacterized protein AMW76695.1 BBF10K_000378
MMSYN1_0836 AMW76696.1 BBF10K_000379
MMSYN1_0837 AMW76697.1 BBF10K_000380
MMSYN1_0838 ~23S rRNA (Guanosine(2251)-2'-O)-methyltransferase RlmB AMW76698.1 BBF10K_000381
MMSYN1_0839 Preprotein translocase subunit SecE AMW76699.1 BBF10K_000382
MMSYN1_0840 Transcription termination/antitermination protein NusG AMW76700.1 BBF10K_000383
MMSYN1_0853 Uncharacterized protein AMW76703.1 BBF10K_000384
MMSYN1_0870 YfcC family protein AMW76705.1 BBF10K_000386
MMSYN1_0872 Ribosome-binding ATPase YchF AMW76706.1 BBF10K_000387
MMSYN1_0874 AMW76708.1 BBF10K_000388
MMSYN1_0875 AMW76709.1 BBF10K_000389
MMSYN1_0876 AMW76710.1 BBF10K_000390
MMSYN1_0877 AMW76711.1 BBF10K_000391
MMSYN1_0879 AMW76713.1 BBF10K_000393
MMSYN1_0881 AMW76714.1 BBF10K_000394
MMSYN1_0885 tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG AMW76715.1 BBF10K_000395
MMSYN1_0886 Dicarboxylate/amino acid:cation symporter AMW76716.1 BBF10K_000396
MMSYN1_0887 CoA-disulfide reductase AMW76717.1 BBF10K_000397
MMSYN1_0906 Choline/ethanolamine kinase AMW76718.1 BBF10K_000398
MMSYN1_0907 Cof-type HAD-IIB family hydrolase AMW76719.1 BBF10K_000399
MMSYN1_0908 Membrane protein insertase YidC AMW76720.1 BBF10K_000400
MMSYN1_0648 50S ribosomal protein L36 AMW76597.1 BBF10K_000402
MMSYN1_0689 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit C AMW76627.1 BBF10K_000403
MMSYN1_0910 50S ribosomal protein L34 AMW76722.1 BBF10K_000404
MMSYN1_0235 AMW76390.1 BBF10K_000405
MMSYN1_0669 50S ribosomal protein L23 AMW76618.1 BBF10K_000407
MMSYN1_0315 Uncharacterized protein AMW76431.1 BBF10K_000408
MMSYN1_0774 Protein-export membrane protein SecG AMW76651.1 BBF10K_000409
MMSYN1_0482 30S ribosomal protein S21 AMW76530.1 BBF10K_000410
MMSYN1_0299 DUF448 domain-containing protein AMW76422.1 BBF10K_000411
MMSYN1_0499 50S ribosomal protein L27 AMW76535.1 BBF10K_000413
MMSYN1_0851 Putative lipoprotein AMW76701.1 BBF10K_000414
MMSYN1_0379 AMW76465.1 BBF10K_000415
MMSYN1_0137 AMW76345.1 BBF10K_000417
MMSYN1_0362 30S ribosomal protein S16 AMW76453.1 BBF10K_000419
MMSYN1_0350 HU family DNA-binding protein AMW76447.1 BBF10K_000421
MMSYN1_0873 Uncharacterized protein AMW76707.1 BBF10K_000422
MMSYN1_0694 HPr family phosphocarrier protein AMW76632.1 BBF10K_000423
MMSYN1_0667 AMW76616.1 BBF10K_000425
MMSYN1_0621 AMW76582.1 BBF10K_000426
MMSYN1_0294 30S ribosomal protein S15 AMW76418.1 BBF10K_000427
MMSYN1_0080 Uncharacterized protein AMW76322.1 BBF10K_000428
MMSYN1_0649 Translation initiation factor IF-1 AMW76598.1 BBF10K_000430
MMSYN1_0830 Uncharacterized protein AMW76690.1 BBF10K_000431
MMSYN1_0662 30S ribosomal protein S17 AMW76611.1 BBF10K_000433
MMSYN1_0852 Uncharacterized protein AMW76702.1 BBF10K_000434
MMSYN1_0778 Uncharacterized protein AMW76655.1 BBF10K_000435
MMSYN1_0797 AMW76666.1 BBF10K_000436
MMSYN1_0317 Uncharacterized protein AMW76433.1 BBF10K_000437
MMSYN1_0082 AMW76324.1 BBF10K_000438
JCVISYN3A_0520 AB hydrolase-1 domain-containing protein N/A BBF10K_003226
JCVISYN3A_0521 Cell division protein SepF N/A BBF10K_003227
JCVISYN3A_0522 N/A BBF10K_003228
JCVISYN3A_0527 Uncharacterized protein N/A BBF10K_003229
JCVISYN3A_0538 Uncharacterized protein N/A BBF10K_003230
JCVISYN3A_0548 N/A BBF10K_003231
JCVISYN3A_0549 dITP/XTP pyrophosphatase N/A BBF10K_003232
JCVISYN3A_0592 N/A BBF10K_003233
JCVISYN3A_0593 Uncharacterized protein N/A BBF10K_003234
JCVISYN3A_0604 LemA family protein N/A BBF10K_003236
JCVISYN3A_0605 Uncharacterized protein N/A BBF10K_003237
JCVISYN3A_0610 Formamidopyrimidine-DNA glycosylase N/A BBF10K_003238
JCVISYN3A_0622 Uncharacterized protein N/A BBF10K_003239
JCVISYN3A_0623 N/A BBF10K_003240

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Name Contact Country
Neil Singh stuelke, joerg Germany
Benjamin Chavez chavez {at} ipk-gatersleben {dot} de Germany
Amjed Alsultan amjed.talib {at} qu {dot} edu {dot} iq Iraq
Kevin Correia kevin {at} kedalionlabs {dot} com Netherlands
Tania Pozzo tania.pozzo {at} gmail {dot} com Sweden
Swaraj Kunal swaraj.avisa {at} gmail {dot} com India
Ery Fukushima ery.fukushima {at} ikiam {dot} edu {dot} ec Ecuador
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org United States
Andrei Sakai andrei.sakai {at} ru {dot} nl Netherlands
Manish Kushwaha manish.kushwaha {at} inrae {dot} fr France
George Church gchurch {at} genetics {dot} med {dot} harvard {dot} edu United States
Dong Nguyen Tam NA Vietnam
James Saenz james.saenz {at} tu-dresden {dot} de Germany
Majed Alghamdi mafa2 {at} le {dot} ac {dot} uk United Kingdom
TING-WEI LIN weitinglin66 {at} gmail {dot} com Taiwan

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