Glowing Plant

Glowing Plant

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A 3 enzyme pathway coding for fungal bioluminescence, codon optimized for Nicotiana benthamiana in MoClo-compatible high-copy ampicillin resistance backbones. 

One of the most requested and inspiring biotech projects has been to make glowing plants. Recently, a group out of Moscow has successfully created a glowing plant that is visible with the naked eye. They were able to do this by using the fungal bioluminescence pathway instead of the bacterial bioluminescence pathway, which increased the efficiency of light production in these eukaryotic organisms. 

To learn more, please see the submitted FreeGenes memo, synthesis memos, and other docs:glowing_plant_memo.docxglowing_plant_synthesis_memo.pdf,  

Documentation
This distribution comprises of {13} genetic parts. {7} of these parts are Luz and Luz alternatives, the main light producing enzyme. {3} of these parts are 2 enzymes that complete the biosynthetic pathway necessary to sustain light production. 1 enzyme, HispS, has been split into 2 parts because it was too large for synthesis directly. It can be put back together with BglII or BsaI with the overhang sequence of GATC. The remaining {3} parts are vector components to put test these genes quickly while a FreeGenes Plant toolkit is put together.

Full history of modifications are available in the snapgene files in sequence_files.zip. Genbank files are also provided in genbank_files.zip. The exact sequences ordered from Twist are available in glowing_plant_order.csv. The genes are flanked by experimentally validated orthogonal primer pairs, and a I-SceI barcode. Full set of primer pairs for all FreeGenes parts are in a git repo. Detailed information on changes can be found in the curators_notes.md. The plate map can be found here.

Quality control documentation is also attached <todo>.

# IP Rights
Planta has filed a provisional patent for use of Luz in plants, and so materials are provided as research use only. 

 

This product is made available under the unilateral OpenMTA

Genes

Gene Name
Luz Fungal Luciferase
Luz-Homologue Fungal Luciferase-Homologue
Luz-Mc Mycena citricolor Fungal Luciferase
Luz-Oo Omphalotus olearius Fungal Luciferase
Luz-RFP Fungal Luciferase RFP fusion
Luz-Y189F Fungal Luciferase Y189F pocket mutation
Luz-Y233F Fungal Luciferase Y233F pocket mutation
H3H hispidin-3-hydroxylase
HispS_1 hispidin synthase
HispS_2 hispidin synthase
Vector-Luz Luz test vector
Vector-HispS HispS test vector
Vector-H3H H3H test vector
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