Glowing Plant

Glowing Plant

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A 3 enzyme pathway coding for fungal bioluminescence, codon optimized for Nicotiana benthamiana in MoClo-compatible high-copy ampicillin resistance backbones. 

One of the most requested and inspiring biotech projects has been to make glowing plants. Recently, a group out of Moscow has successfully created a glowing plant that is visible with the naked eye. They were able to do this by using the fungal bioluminescence pathway instead of the bacterial bioluminescence pathway, which increased the efficiency of light production in these eukaryotic organisms. 

To learn more, please see the submitted FreeGenes memo, synthesis memos, and other docs:glowing_plant_memo.docxglowing_plant_synthesis_memo.pdf,  

Documentation

This distribution comprises of {13} genetic parts. {7} of these parts are Luz and Luz alternatives, the main light producing enzyme. {3} of these parts are 2 enzymes that complete the biosynthetic pathway necessary to sustain light production. 1 enzyme, HispS, has been split into 2 parts because it was too large for synthesis directly. It can be put back together with BglII or BsaI with the overhang sequence of GATC. The remaining {3} parts are vector components to put test these genes quickly while a FreeGenes Plant toolkit is put together.      

The genes present on the plate are available in E. coli glycerol stocks and as purified nucleic acids. Please specify in the order notes if you need a particular format. The plate map can be found here.

Full history of modifications are available in the snapgene files in sequence_files.zip. Genbank files are also provided in genbank_files.zip. The exact sequences ordered from Twist are available in glowing_plant_order.csv. The genes are flanked by experimentally validated orthogonal primer pairs, and a I-SceI barcode. Full set of primer pairs for all FreeGenes parts are in a git repo. Detailed information on changes can be found in the curators_notes.md.


This product is made available under the unilateral OpenMTA

Some or all of these items are for use only as permitted by a research exemption.

 

Genes

Gene Name Freegenes ID
Luz Fungal Luciferase BBF10K_003480
Luz-Homologue Fungal Luciferase-Homologue BBF10K_003481
Luz-Mc Mycena citricolor Fungal Luciferase BBF10K_003482
Luz-Oo Omphalotus olearius Fungal Luciferase BBF10K_003483
Luz-RFP Fungal Luciferase RFP fusion BBF10K_003484
Luz-Y189F Fungal Luciferase Y189F pocket mutation BBF10K_003485
Luz-Y233F Fungal Luciferase Y233F pocket mutation BBF10K_003486
H3H hispidin-3-hydroxylase BBF10K_003487
HispS_1 hispidin synthase BBF10K_003488
HispS_2 hispidin synthase BBF10K_003489
Vector-Luz Luz test vector BBF10K_003490
Vector-HispS HispS test vector BBF10K_003491
Vector-H3H H3H test vector BBF10K_003492

Download all of this information as a CSV from our GitHub.

Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact Country
Eric Theis Aracaifon {at} gmail {dot} com United States
Travis Wertz travis {at} traviswertz {dot} com United States
Amjed Alsultan amjed.talib {at} qu {dot} edu {dot} iq Iraq
Ery Fukushima ery.fukushima {at} ikiam {dot} edu {dot} ec Ecuador
Majed Alghamdi mafa2 {at} le {dot} ac {dot} uk United Kingdom
Dong Nguyen Tam NA Vietnam
Ahmed Atef ahmed_atefaig2 {at} yahoo {dot} com United States
James Altamirano James_altam {at} yahoo {dot} com United States
Ahmed Hegazy a.hegazy {at} biotec {dot} rwth-aachen {dot} de Germany
Erman ÖZER aracnoid {at} gmail {dot} com Turkey
Ben Daniels bndaniel {at} calpoly {dot} edu United States
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org United States
Kitty Martens kitmartens {at} gmail {dot} com Canada
Angelica Hernandez sofiash.me {at} gmail {dot} com Mexico

Download all of this information as a CSV from our GitHub.
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