
Are you looking for an all-in-one toolkit to build up your dream construct to try expressing your enzymes of choice, tailored to your lab’s resources and needs? A toolkit containing different promoters, RBSs, linkers, tags, reporters? This collection might be for you.
A collection of >100 DNA parts designed to be assembled in one-pot reaction using BsaI-based Golden-Gate assembly. DNA parts are MoClo, SEVA, Loop and CIDAR assembly compatible. The collection includes:
- 3 promoters
- 7 RBS and insulators
- 9 purification tags, 3 localization signals
- 5 cleavage sites, flexible linkers
- 23 genes of which 5 are reporters and 18 are used in diagnostics
- 3 destination vectors
The Protein Expression Toolkit is shipped as purified and dried down plasmid DNA stained with Cresol Red in two 96-well plates. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
What can it be used for:
Use the DNA parts of the collection to assemble the expression plasmids for the protein of your choice, tailored to the resources present in your lab. The collection enables thousands of combinations for each gene of choice. Full power of the collection can be explored using pipetting robots. The collection is compatible with all the CDSs present in the Open Enzyme collections.
Assembly Scheme
If you've already received the kit, check out the layout for Plate 1 and Plate 2.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
If you are interested in requesting additional parts to be added to this toolkit, complete this request form.
Genes
Gene | Name | NCBI ID | Freegenes ID |
---|---|---|---|
CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | BBF10K_003332 | |
CE_sarcGFP | CE_sarcGFP | BBF10K_003356 | |
CE_beta-galactosidase | CE_beta-galactosidase | BBF10K_003366 | |
AF_lacZ pDest | AF_lacZ pDest | N/A | BBF10K_003373 |
AE_lacZ pDest | AE_lacZ pDest | N/A | BBF10K_003375 |
AF_high copy (pUC) ori/KanR | AF_high copy (pUC) ori/KanR | N/A | BBF10K_003376 |
AF_medium copy (pET) ori/KanR | AF_medium copy (pET) ori/KanR | N/A | BBF10K_003377 |
AB_T7 | AB_T7 | N/A | BBF10K_003378 |
AB_T7_lacO | AB_T7_lacO | N/A | BBF10K_003379 |
AB_pTac | AB_pTac | N/A | BBF10K_003380 |
BT1_BCD2 | BT1_BCD2 | N/A | BBF10K_003384 |
BT1_Riboj54_F1 | BT1_Riboj54_F1 | N/A | BBF10K_003385 |
BT1_Riboj_B34 | BT1_Riboj_B34 | N/A | BBF10K_003386 |
BT1_BCD12 | BT1_BCD12 | N/A | BBF10K_003387 |
BT1_BCD23 | BT1_BCD23 | N/A | BBF10K_003388 |
BT1_BCD2_His | BT1_BCD2_His | N/A | BBF10K_003389 |
BT1_RiboJ_B34_His | BT1_RiboJ_B34_His | N/A | BBF10K_003390 |
BT1_BCD2_DsbA | BT1_BCD2_DsbA | N/A | BBF10K_003391 |
BT1_BCD2_OmpT | BT1_BCD2_OmpT | N/A | BBF10K_003392 |
BT1_BCD2_pelB | BT1_BCD2_pelB | N/A | BBF10K_003393 |
T1T2_Calm | T1T2_Calm | N/A | BBF10K_003395 |
T1T2_CBD | T1T2_CBD | N/A | BBF10K_003396 |
T1T2_Cex | T1T2_Cex | N/A | BBF10K_003397 |
T1T2_MBP | T1T2_MBP | N/A | BBF10K_003398 |
T1T2_R5 | T1T2_R5 | N/A | BBF10K_003399 |
T1T2_GST | T1T2_GST | N/A | BBF10K_003400 |
T1T2_CipA | T1T2_CipA | N/A | BBF10K_003401 |
T1T2_S-Tag | T1T2_S-Tag | N/A | BBF10K_003402 |
T1T2_GB1 | T1T2_GB1 | N/A | BBF10K_003403 |
T1T2_GS flexible | T1T2_GS flexible | N/A | BBF10K_003404 |
T1T3_GS flexible | T1T3_GS flexible | N/A | BBF10K_003405 |
T1C_GS flexible | T1C_GS flexible | N/A | BBF10K_003406 |
T1C_His_TEVcut | T1C_His_TEVcut | N/A | BBF10K_003407 |
BT2_BCD2_His | BT2_BCD2_His | N/A | BBF10K_003408 |
BT2_RiboJ_B34 | BT2_RiboJ_B34 | N/A | BBF10K_003409 |
BC_B0034 | BC_B0034 | N/A | BBF10K_003410 |
BC_B0032 | BC_B0032 | N/A | BBF10K_003411 |
BC_RiboJ_RBS64 | BC_RiboJ_RBS64 | N/A | BBF10K_003412 |
BC_RiboJ_B34 | BC_RiboJ_B34 | N/A | BBF10K_003413 |
BC_BCD23 | BC_BCD23 | N/A | BBF10K_003414 |
BC_BCD12 | BC_BCD12 | N/A | BBF10K_003415 |
BC_BCD2 | BC_BCD2 | N/A | BBF10K_003416 |
T2C_GS flexible | T2C_GS flexible | N/A | BBF10K_003417 |
T2C_TEVcut | T2C_TEVcut | N/A | BBF10K_003418 |
BC_BCD2_His_TEVcut | BC_BCD2_His_TEVcut | N/A | BBF10K_003419 |
BC_BCD2_His_fuGFP_TEVcut | BC_BCD2_His_fuGFP_TEVcut | N/A | BBF10K_003420 |
T2T3_int_∆I | T2T3_int_∆I | BBF10K_003421 | |
T2T3_SUMO | T2T3_SUMO | BBF10K_003422 | |
T2T3_fuGFP | T2T3_fuGFP | BBF10K_003423 | |
T2T3_AmilCP | T2T3_AmilCP | BBF10K_003424 | |
T2T3_GS flexible | T2T3_GS flexible | BBF10K_003425 | |
T3C_SUMO | T3C_SUMO | BBF10K_003426 | |
T3C_TEV | T3C_TEV | BBF10K_003427 | |
T3C_int_∆I | T3C_int_∆I | BBF10K_003428 | |
T3C_fuGFP | T3C_fuGFP | BBF10K_003429 | |
T3C_GS flexible linker | T3C_GS flexible linker | N/A | BBF10K_003430 |
CD_OMMLV | CD_OMMLV | BBF10K_003431 | |
CD_HIVRT | CD_HIVRT | BBF10K_003432 | |
CD_Bst-LF | CD_Bst-LF | BBF10K_003433 | |
CD_OpenVent | CD_OpenVent | BBF10K_003434 | |
CD_RNAseInhibitor | CD_RNAseInhibitor | BBF10K_003436 | |
CD_gp32 | CD_gp32 | BBF10K_003437 | |
CD_BsuLF | CD_BsuLF | BBF10K_003438 | |
CD_UvsY | CD_UvsY | BBF10K_003439 | |
CD_UvsX | CD_UvsX | BBF10K_003440 | |
CE_T7RNApol_Stop | CE_T7RNApol_Stop | BBF10K_003441 | |
CD_PBCV-1_ligase | CD_PBCV-1_ligase | BBF10K_003442 | |
CD_HRP | CD_HRP | BBF10K_003443 | |
CD_TEV_protease | CD_TEV_protease | BBF10K_003444 | |
CD_SUMO_protease | CD_SUMO_protease | BBF10K_003445 | |
CD_Calmodulin | CD_Calmodulin | BBF10K_003446 | |
CD_fuGFP | CD_fuGFP | BBF10K_003447 | |
CD_AmilCP | CD_AmilCP | BBF10K_003448 | |
CD_TthRNAseH | CD_TthRNAseH | BBF10K_003449 | |
CD_EcRNAseH | CD_EcRNAseH | BBF10K_003450 | |
DT4_Int_SaltS | DT4_Int_SaltS | N/A | BBF10K_003451 |
DT4_Int_ThiolS | DT4_Int_ThiolS | N/A | BBF10K_003452 |
DT4_TEV | DT4_TEV | N/A | BBF10K_003453 |
DT4_fuGFP | DT4_fuGFP | N/A | BBF10K_003454 |
DT4_GS flexible linker | DT4_GS flexible linker | N/A | BBF10K_003455 |
DT5_TEVcut | DT5_TEVcut | N/A | BBF10K_003457 |
DF_TEVcut_His_Stop_TZ | DF_TEVcut_His_Stop_TZ | N/A | BBF10K_003459 |
DF_TEVcut_fuGFP_His_Stop_TZ | DF_TEVcut_fuGFP_His_Stop_TZ | N/A | BBF10K_003460 |
T4T5_Int_ThiolS | T4T5_Int_ThiolS | N/A | BBF10K_003461 |
T4T5_Int_SaltS | T4T5_Int_SaltS | N/A | BBF10K_003462 |
T4T5_AmilCP | T4T5_AmilCP | N/A | BBF10K_003463 |
T4T5_fuGFP | T4T5_fuGFP | N/A | BBF10K_003464 |
T4T5_GS flexible | T4T5_GS flexible | N/A | BBF10K_003465 |
T4E_3xStop | T4E_3xStop | N/A | BBF10K_003466 |
T5E_6H | T5E_6H | N/A | BBF10K_003467 |
T5E_Calm | T5E_Calm | N/A | BBF10K_003468 |
T5E_CBD | T5E_CBD | N/A | BBF10K_003469 |
T5E_Cex | T5E_Cex | N/A | BBF10K_003470 |
T5E_MBP | T5E_MBP | N/A | BBF10K_003471 |
T5E_R5 | T5E_R5 | N/A | BBF10K_003472 |
T5E_CipA | T5E_CipA | N/A | BBF10K_003473 |
T5E_3xStop | T5E_3xStop | N/A | BBF10K_003474 |
EF_ECK9600 | EF_ECK9600 | N/A | BBF10K_003475 |
EF_T7term | EF_T7term | N/A | BBF10K_003476 |
EF_TZ | EF_TZ | N/A | BBF10K_003477 |
Download all of this information as a CSV from our GitHub.
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.Name | Contact | Country |
---|---|---|
Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
Mariela Escobar | maru {at} michroma {dot} co | Argentina |
Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
Tri Ngo | NA | United States |
Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
Amjed Alsultan | amjed.talib {at} qu {dot} edu {dot} iq | Iraq |
Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
Kyle Erlenbeck | krerlenbeck {at} dcsdk12 {dot} org | United States |
Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
Patrik D'haeseleer | patrikd {at} gmail {dot} com | United States |
Alexandr Dorif | dorif11 {at} gmail {dot} com | Moldova, Republic of |
Vitaliy Strochkov | NA | Kazakhstan |
Dong Nguyen Tam | NA | Vietnam |
Muhammad Shoaib | mshoaib {at} lums {dot} edu {dot} pk | Pakistan |
Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
Ferdinand Molnár | NA | Kazakhstan |
Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
Vincent Paris | NA | United States |
Ery Fukushima | ery.fukushima {at} ikiam {dot} edu {dot} ec | Ecuador |
Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
Stephen Klusza | smklusza {at} gmail {dot} com | United States |
Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
Jennifer Hsieh | j.hsieh {at} kurabiotech {dot} com | Chile |
semih cagan | semihcgn {at} gmail {dot} com | Turkey |
Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
Shivang Joshi | https://www.bacto.bio | United Kingdom |
Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
James Altamirano | James_altam {at} yahoo {dot} com | United States |
Jose David Rosales | https://www.linkedin.com/in/david-rosales-574a7151/ | United States |
Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
Shu Li | shu {at} petribio {dot} com | United States |
Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
Andrei Berasneu | andreyberesnevson {at} gmail {dot} com | Poland |
Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
Juan Sebastian Alvarez | juan.alvarez1 {at} ucalgary {dot} ca | Canada |
Emin Bursa | NA | United States |
TING-WEI LIN | weitinglin66 {at} gmail {dot} com | Taiwan |
Stephen Klusza | StephenKlusza {at} clayton {dot} edu | United States |
Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
Aileen Button | acbutton {at} ucsd {dot} edu | United States |
Ashton Cropp | tacropp {at} vcu {dot} edu | United States |
Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
Matias Cabeza | matiascabeza {at} gmail {dot} com | Argentina |
Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
Kheng Oon Low | khengoon {at} nibm {dot} my | Malaysia |
Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
Rodrigo Caroca | rcaroca {at} uazuay {dot} edu {dot} ec | Ecuador |
Nicholas White | nickk.white {at} gmail {dot} com | United States |
Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
Peer Schenk | Reuben.brown {at} uq {dot} edu {dot} au | Australia |
Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
Peter Rootes | root0059 {at} umn {dot} edu | United States |
Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
Krishna Gupta | gupta-krishna {at} ipfdd {dot} de | Germany |
Download all of this information as a CSV from our GitHub.