Are you looking for an all-in-one toolkit to build up your dream construct to try expressing your enzymes of choice, tailored to your lab’s resources and needs? A toolkit containing different promoters, RBSs, linkers, tags, reporters? This collection might be for you.
A collection of >100 DNA parts designed to be assembled in one-pot reaction using BsaI-based Golden-Gate assembly. DNA parts are MoClo, SEVA, Loop and CIDAR assembly compatible. The collection includes:
- 3 promoters
- 7 RBS and insulators
- 9 purification tags, 3 localization signals
- 5 cleavage sites, flexible linkers
- 23 genes of which 5 are reporters and 18 are used in diagnostics
- 3 destination vectors
The Protein Expression Toolkit is shipped as purified and dried down plasmid DNA stained with Cresol Red in two 96-well plates. Each well contains approximately 50 ng of DNA. Cresol Red will not impact plasmid transformation.
What can it be used for:
Use the DNA parts of the collection to assemble the expression plasmids for the protein of your choice, tailored to the resources present in your lab. The collection enables thousands of combinations for each gene of choice. Full power of the collection can be explored using pipetting robots. The collection is compatible with all the CDSs present in the Open Enzyme collections.
Assembly Scheme
If you've already received the kit, check out the layout for Plate 1 and Plate 2.
This kit is included in kit plate 2 of the 2022 iGEM distribution. The plate map for kit plate 2 of the iGEM 2022 distribution can be found here.
This product is made available under the unilateral OpenMTA.
Some or all of these items are for use only as permitted by a research exemption.
If you are interested in requesting additional parts to be added to this toolkit, complete this request form.
Genes
| Gene | Name | NCBI ID | Freegenes ID |
|---|---|---|---|
| CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | CE_meffBlue/Rtms5/ NF pocilloporin /Biobrick K1033902 | BBF10K_003332 | |
| CE_sarcGFP | CE_sarcGFP | BBF10K_003356 | |
| CE_beta-galactosidase | CE_beta-galactosidase | BBF10K_003366 | |
| AF_lacZ pDest | AF_lacZ pDest | N/A | BBF10K_003373 |
| AE_lacZ pDest | AE_lacZ pDest | N/A | BBF10K_003375 |
| AF_high copy (pUC) ori/KanR | AF_high copy (pUC) ori/KanR | N/A | BBF10K_003376 |
| AF_medium copy (pET) ori/KanR | AF_medium copy (pET) ori/KanR | N/A | BBF10K_003377 |
| AB_T7 | AB_T7 | N/A | BBF10K_003378 |
| AB_T7_lacO | AB_T7_lacO | N/A | BBF10K_003379 |
| AB_pTac | AB_pTac | N/A | BBF10K_003380 |
| BT1_BCD2 | BT1_BCD2 | N/A | BBF10K_003384 |
| BT1_Riboj54_F1 | BT1_Riboj54_F1 | N/A | BBF10K_003385 |
| BT1_Riboj_B34 | BT1_Riboj_B34 | N/A | BBF10K_003386 |
| BT1_BCD12 | BT1_BCD12 | N/A | BBF10K_003387 |
| BT1_BCD23 | BT1_BCD23 | N/A | BBF10K_003388 |
| BT1_BCD2_His | BT1_BCD2_His | N/A | BBF10K_003389 |
| BT1_RiboJ_B34_His | BT1_RiboJ_B34_His | N/A | BBF10K_003390 |
| BT1_BCD2_DsbA | BT1_BCD2_DsbA | N/A | BBF10K_003391 |
| BT1_BCD2_OmpT | BT1_BCD2_OmpT | N/A | BBF10K_003392 |
| BT1_BCD2_pelB | BT1_BCD2_pelB | N/A | BBF10K_003393 |
| T1T2_Calm | T1T2_Calm | N/A | BBF10K_003395 |
| T1T2_CBD | T1T2_CBD | N/A | BBF10K_003396 |
| T1T2_Cex | T1T2_Cex | N/A | BBF10K_003397 |
| T1T2_MBP | T1T2_MBP | N/A | BBF10K_003398 |
| T1T2_R5 | T1T2_R5 | N/A | BBF10K_003399 |
| T1T2_GST | T1T2_GST | N/A | BBF10K_003400 |
| T1T2_CipA | T1T2_CipA | N/A | BBF10K_003401 |
| T1T2_S-Tag | T1T2_S-Tag | N/A | BBF10K_003402 |
| T1T2_GB1 | T1T2_GB1 | N/A | BBF10K_003403 |
| T1T2_GS flexible | T1T2_GS flexible | N/A | BBF10K_003404 |
| T1T3_GS flexible | T1T3_GS flexible | N/A | BBF10K_003405 |
| T1C_GS flexible | T1C_GS flexible | N/A | BBF10K_003406 |
| T1C_His_TEVcut | T1C_His_TEVcut | N/A | BBF10K_003407 |
| BT2_BCD2_His | BT2_BCD2_His | N/A | BBF10K_003408 |
| BT2_RiboJ_B34 | BT2_RiboJ_B34 | N/A | BBF10K_003409 |
| BC_B0034 | BC_B0034 | N/A | BBF10K_003410 |
| BC_B0032 | BC_B0032 | N/A | BBF10K_003411 |
| BC_RiboJ_RBS64 | BC_RiboJ_RBS64 | N/A | BBF10K_003412 |
| BC_RiboJ_B34 | BC_RiboJ_B34 | N/A | BBF10K_003413 |
| BC_BCD23 | BC_BCD23 | N/A | BBF10K_003414 |
| BC_BCD12 | BC_BCD12 | N/A | BBF10K_003415 |
| BC_BCD2 | BC_BCD2 | N/A | BBF10K_003416 |
| T2C_GS flexible | T2C_GS flexible | N/A | BBF10K_003417 |
| T2C_TEVcut | T2C_TEVcut | N/A | BBF10K_003418 |
| BC_BCD2_His_TEVcut | BC_BCD2_His_TEVcut | N/A | BBF10K_003419 |
| BC_BCD2_His_fuGFP_TEVcut | BC_BCD2_His_fuGFP_TEVcut | N/A | BBF10K_003420 |
| T2T3_int_∆I | T2T3_int_∆I | BBF10K_003421 | |
| T2T3_SUMO | T2T3_SUMO | BBF10K_003422 | |
| T2T3_fuGFP | T2T3_fuGFP | BBF10K_003423 | |
| T2T3_AmilCP | T2T3_AmilCP | BBF10K_003424 | |
| T2T3_GS flexible | T2T3_GS flexible | BBF10K_003425 | |
| T3C_SUMO | T3C_SUMO | BBF10K_003426 | |
| T3C_TEV | T3C_TEV | BBF10K_003427 | |
| T3C_int_∆I | T3C_int_∆I | BBF10K_003428 | |
| T3C_fuGFP | T3C_fuGFP | BBF10K_003429 | |
| T3C_GS flexible linker | T3C_GS flexible linker | N/A | BBF10K_003430 |
| CD_OMMLV | CD_OMMLV | BBF10K_003431 | |
| CD_HIVRT | CD_HIVRT | BBF10K_003432 | |
| CD_Bst-LF | CD_Bst-LF | BBF10K_003433 | |
| CD_OpenVent | CD_OpenVent | BBF10K_003434 | |
| CD_RNAseInhibitor | CD_RNAseInhibitor | BBF10K_003436 | |
| CD_gp32 | CD_gp32 | BBF10K_003437 | |
| CD_BsuLF | CD_BsuLF | BBF10K_003438 | |
| CD_UvsY | CD_UvsY | BBF10K_003439 | |
| CD_UvsX | CD_UvsX | BBF10K_003440 | |
| CE_T7RNApol_Stop | CE_T7RNApol_Stop | BBF10K_003441 | |
| CD_PBCV-1_ligase | CD_PBCV-1_ligase | BBF10K_003442 | |
| CD_HRP | CD_HRP | BBF10K_003443 | |
| CD_TEV_protease | CD_TEV_protease | BBF10K_003444 | |
| CD_SUMO_protease | CD_SUMO_protease | BBF10K_003445 | |
| CD_Calmodulin | CD_Calmodulin | BBF10K_003446 | |
| CD_fuGFP | CD_fuGFP | BBF10K_003447 | |
| CD_AmilCP | CD_AmilCP | BBF10K_003448 | |
| CD_TthRNAseH | CD_TthRNAseH | BBF10K_003449 | |
| CD_EcRNAseH | CD_EcRNAseH | BBF10K_003450 | |
| DT4_Int_SaltS | DT4_Int_SaltS | N/A | BBF10K_003451 |
| DT4_Int_ThiolS | DT4_Int_ThiolS | N/A | BBF10K_003452 |
| DT4_TEV | DT4_TEV | N/A | BBF10K_003453 |
| DT4_fuGFP | DT4_fuGFP | N/A | BBF10K_003454 |
| DT4_GS flexible linker | DT4_GS flexible linker | N/A | BBF10K_003455 |
| DT5_TEVcut | DT5_TEVcut | N/A | BBF10K_003457 |
| DF_TEVcut_His_Stop_TZ | DF_TEVcut_His_Stop_TZ | N/A | BBF10K_003459 |
| DF_TEVcut_fuGFP_His_Stop_TZ | DF_TEVcut_fuGFP_His_Stop_TZ | N/A | BBF10K_003460 |
| T4T5_Int_ThiolS | T4T5_Int_ThiolS | N/A | BBF10K_003461 |
| T4T5_Int_SaltS | T4T5_Int_SaltS | N/A | BBF10K_003462 |
| T4T5_AmilCP | T4T5_AmilCP | N/A | BBF10K_003463 |
| T4T5_fuGFP | T4T5_fuGFP | N/A | BBF10K_003464 |
| T4T5_GS flexible | T4T5_GS flexible | N/A | BBF10K_003465 |
| T4E_3xStop | T4E_3xStop | N/A | BBF10K_003466 |
| T5E_6H | T5E_6H | N/A | BBF10K_003467 |
| T5E_Calm | T5E_Calm | N/A | BBF10K_003468 |
| T5E_CBD | T5E_CBD | N/A | BBF10K_003469 |
| T5E_Cex | T5E_Cex | N/A | BBF10K_003470 |
| T5E_MBP | T5E_MBP | N/A | BBF10K_003471 |
| T5E_R5 | T5E_R5 | N/A | BBF10K_003472 |
| T5E_CipA | T5E_CipA | N/A | BBF10K_003473 |
| T5E_3xStop | T5E_3xStop | N/A | BBF10K_003474 |
| EF_ECK9600 | EF_ECK9600 | N/A | BBF10K_003475 |
| EF_T7term | EF_T7term | N/A | BBF10K_003476 |
| EF_TZ | EF_TZ | N/A | BBF10K_003477 |
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Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.| Name | Contact | Country |
|---|---|---|
| Amitabha Majumdar | Mamitava {at} nccs {dot} res {dot} in | India |
| Alexandr Dorif | dorif11 {at} gmail {dot} com | Moldova, Republic of |
| Lisandro Pacheco | lpacheco28 {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| Kheng Oon Low | khengoon {at} nibm {dot} my | Malaysia |
| Juan Sebastian Alvarez | juan.alvarez1 {at} ucalgary {dot} ca | Canada |
| Juan Vicente Farizano | juan.farizano {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Daniel Fernandez | NA | United States |
| elwi machado | elwi.machado {at} unisimonbolivar {dot} edu {dot} co | Colombia |
| Cihan Aydin | cihan.aydin {at} medeniyet {dot} edu {dot} tr | Turkey |
| Amjed Alsultan | amjed.talib {at} qu {dot} edu {dot} iq | Iraq |
| James Altamirano | James_altam {at} yahoo {dot} com | United States |
| Nicholas White | nickk.white {at} gmail {dot} com | United States |
| Arthur Zanetti Nunes Fernandes | fgueiros {at} iq {dot} usp {dot} br | United States |
| Kyle Erlenbeck | krerlenbeck {at} dcsdk12 {dot} org | United States |
| Jennifer Hsieh | j.hsieh {at} kurabiotech {dot} com | Chile |
| Josephine Labos | N/A | Philippines |
| Prince Samoh | Samohprince.edem {at} gmail {dot} com | Ghana |
| Aleksandr Shilovich | mercurialbadger {at} yandex {dot} ru | Russia |
| Shu Li | shu {at} petribio {dot} com | United States |
| Emre Yörük | unalsergen {at} gmail {dot} com | Turkey |
| LUN CUI | luncui {at} cczu {dot} edu {dot} cn | China |
| robson tramontina | robson.tramontina {at} gmail {dot} com | Brazil |
| Ery Fukushima | ery.fukushima {at} ikiam {dot} edu {dot} ec | Ecuador |
| Ben Thuronyi | bwt2 {at} williams {dot} edu | United States |
| Matthew Phanchana | matthew.pha {at} mahidol {dot} edu | Thailand |
| Michael Miller | michael.j.miller {at} uon {dot} edu {dot} au | Australia |
| Shivang Joshi | https://www.bacto.bio | United Kingdom |
| Shree Ram Singh | singhlabasu {at} gmail {dot} com | United States |
| TING-WEI LIN | weitinglin66 {at} gmail {dot} com | Taiwan |
| Muhammad Shoaib | mshoaib {at} lums {dot} edu {dot} pk | Pakistan |
| Aileen Button | acbutton {at} ucsd {dot} edu | United States |
| Esteban Erben | eerben {at} iib {dot} unsam {dot} edu {dot} ar | Argentina |
| Dayananda Chandrappa | e2ebiotech {at} gmail {dot} com | India |
| Tri Ngo | NA | United States |
| William Fried | uscigem {at} usc {dot} edu | United States |
| Ahmad Suparmin | ahmad.suparmin {at} ugm {dot} ac {dot} id | Indonesia |
| Simran Kushwaha | https://www.bits-pilani.ac.in/pilani/biologicalScience/Faculty | India |
| Tomasz Jurkowski | jurkowskit {at} cardiff {dot} ac {dot} uk | United Kingdom |
| Wei-Min Chang | WeiMinChang {at} tmu {dot} edu {dot} tw | Taiwan |
| Ahmed Hegazy | a.hegazy {at} biotec {dot} rwth-aachen {dot} de | Germany |
| Nick McGregor | mcgregn2 {at} gmail {dot} com | Canada |
| Foong-Jing Goh | wind1989 {at} hotmail {dot} com | Taiwan |
| Han Teng Wong | wong_han_teng {at} imcb {dot} a-star {dot} edu {dot} sg | Singapore |
| Stephen Klusza | smklusza {at} gmail {dot} com | United States |
| Patrik D'haeseleer | patrikd {at} gmail {dot} com | United States |
| Nikolaus Dietz | nikolaus.dietz {at} unibas {dot} ch | Switzerland |
| Jimmy Linhares | Synbioamazonas {at} gmail {dot} com | Brazil |
| Kelly Gallagher | kelly.gallagher {at} oneonta {dot} edu | United States |
| Andrei Berasneu | andreyberesnevson {at} gmail {dot} com | Poland |
| Erman ÖZER | aracnoid {at} gmail {dot} com | Turkey |
| Vincent Paris | NA | United States |
| Marc Juul | marc {at} juul {dot} io | United States |
| Suresh Arakera | sbarakera {at} kud {dot} ac {dot} in | India |
| Ashton Cropp | tacropp {at} vcu {dot} edu | United States |
| Tocvic Meng | Tocvic Meng \ Email: tocvic.meng {at} holocyte {dot} com \ website https://gmexpression {dot} com/ | Australia |
| Mariela Escobar | maru {at} michroma {dot} co | Argentina |
| Peter Rootes | root0059 {at} umn {dot} edu | United States |
| Ahmed Atef | ahmed_atefaig2 {at} yahoo {dot} com | Saudi Arabia |
| Jacques Mathieu | mathieu {at} rice {dot} edu | United States |
| Moritz Venne | moritz.venne {at} web {dot} de | Germany |
| Rodrigo Caroca | rcaroca {at} uazuay {dot} edu {dot} ec | Ecuador |
| Wapouo Fadanka Stephane | mboalab {at} gmail {dot} com | Republic of Cameroon |
| Jordan Gonzalez | jgonzalez {at} thecitizensciencelab {dot} org | United States |
| Intan Taufik | i.taufik {at} sith {dot} itb {dot} ac {dot} id | Indonesia |
| Anil Challa | akchalla {at} uab {dot} edu | United States |
| Jose Munoz | Jose.Munoz {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Kenneth Frimpong | ken.frimpong {at} thrivusinstitute {dot} edu {dot} gh | Ghana |
| Dong Nguyen Tam | NA | Vietnam |
| Tal Globus | bio {at} talglobus {dot} com | United States |
| Tim Dobbs | tim.dobbs {at} cri-paris {dot} org | France |
| Watanyu Bunsermyos | watanyu.bu {at} ku {dot} th | Thailand |
| Emin Bursa | NA | United States |
| Elizabeth Bilsland | bilsland {at} unicamp {dot} br | Brazil |
| Alexander Iakovlev | atom.liquid {at} gmail {dot} com | Canada |
| Majed Alghamdi | mafa2 {at} le {dot} ac {dot} uk | United Kingdom |
| Stephen Klusza | StephenKlusza {at} clayton {dot} edu | United States |
| Fristot Elsa | elsa.fristot {at} cbs {dot} cnrs {dot} fr | France |
| Muzaffar Muminov | muminov.imbio {at} gmail {dot} com | Uzbekistan |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | United States |
| Sreenivas Eadara | sreeni.eadara {at} gmail {dot} com | United States |
| Gülşen Günel | gulsen.gunel {at} ogr {dot} iu {dot} edu {dot} tr | Turkey |
| Jonathan Chevriau | j.chevriau {at} gmail {dot} com | Argentina |
| Matias Cabeza | matiascabeza {at} gmail {dot} com | Argentina |
| Peer Schenk | Reuben.brown {at} uq {dot} edu {dot} au | Australia |
| Meng Lu | menglu {at} iastate {dot} edu | United States |
| Jonathan LeCureux | jlecureu {at} svsuedu | United States |
| Jose David Rosales | https://www.linkedin.com/in/david-rosales-574a7151/ | United States |
| Keoni Gandall | koeng101 {at} gmail {dot} com | United States |
| Peer Schenk | reuben.brown {at} uqconnect {dot} edu {dot} au | Australia |
| Kefentse Arnold Tumedi | tumediak {at} yahoo {dot} com | Botswana |
| Simon Hofer | soyouwantalab {at} gmail {dot} com | Austria |
| Amir Kashani | NA | Canada |
| Guillermo VegaLopez | guillermo.vega-lopez {at} fbqf {dot} unt {dot} edu {dot} ar | Argentina |
| Manish Kushwaha | manish.kushwaha {at} inrae {dot} fr | France |
| Swaraj Kunal | swaraj.avisa {at} gmail {dot} com | India |
| semih cagan | semihcgn {at} gmail {dot} com | Turkey |
| Pratik Vyas | iampratikvyas {at} gamil {dot} com | India |
| Ferdinand Molnár | NA | Kazakhstan |
| John Allan | john.allan {at} northumbria {dot} ac {dot} uk | United Kingdom |
| Krishna Gupta | gupta-krishna {at} ipfdd {dot} de | Germany |
| Andrew Gray | andrewg {at} bioq {dot} org {dot} au | Australia |
| Benjamin Arias | barias {at} alumni {dot} usfq {dot} edu {dot} ec | Ecuador |
| Yi Zhang | www.pharmozyme.com | United States |
| John Allan | john.allan {at} eng {dot} ox {dot} ac {dot} uk | United Kingdom |
| Vitaliy Strochkov | NA | Kazakhstan |
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