Boolean Integrase Logic (BIL) Gates

Boolean Integrase Logic (BIL) Gates

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What is it?

We developed a three-terminal device architecture, termed the transcriptor, that uses bacteriophage serine integrases to control the flow of RNA polymerase along DNA. Integrase-mediated inversion or deletion of DNA encoding transcription terminators or a promoter modulates transcription rates. We realized permanent amplifying AND, NAND, OR, XOR, NOR, and XNOR gates actuated across common control signal ranges and sequential logic supporting autonomous cell-cell communication of DNA encoding distinct logic-gate states. The single-layer digital logic architecture developed here enables engineering of amplifying logic gates to control transcription rates within and across diverse organisms. 

 

What can it be used for?

Two-input logic and/or data storage in a simple DNA register.  Native or heterologous transcription sources can flip the gates.

 

Where can I find more information?

Amplifying Genetic Logic Gates, Jerome Bonnet Peter Yin Monica E. Ortiz Pakpoom Subsoontorn Drew Endy https://science.sciencemag.org/content/340/6132/599.abstract

 

All genes are present on the plate in E. coli glycerol stocks. If you've already received the plate, check out the plate layout.

 

Genes

Gene Name
AND-gate-V2.0 AND-gate-V2.0
OR gate OR gate
NOR gate-V2.0 NOR gate-V2.0
XOR gate-V2.0 XOR gate-V2.0
Dual-recombinase-controller Dual-recombinase-controller

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Name Contact
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org
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