Boolean Integrase Logic (BIL) Gates

Boolean Integrase Logic (BIL) Gates

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What is it?

We developed a three-terminal device architecture, termed the transcriptor, that uses bacteriophage serine integrases to control the flow of RNA polymerase along DNA. Integrase-mediated inversion or deletion of DNA encoding transcription terminators or a promoter modulates transcription rates. We realized permanent amplifying AND, NAND, OR, XOR, NOR, and XNOR gates actuated across common control signal ranges and sequential logic supporting autonomous cell-cell communication of DNA encoding distinct logic-gate states. The single-layer digital logic architecture developed here enables engineering of amplifying logic gates to control transcription rates within and across diverse organisms. 

 

What can it be used for?

Two-input logic and/or data storage in a simple DNA register.  Native or heterologous transcription sources can flip the gates.

 

Where can I find more information?

Amplifying Genetic Logic Gates, Jerome Bonnet Peter Yin Monica E. Ortiz Pakpoom Subsoontorn Drew Endy https://science.sciencemag.org/content/340/6132/599.abstract

 

All genes present on the plate are available in two formats: E. coli glycerol stocks and purified nucleic acids. Please specify in the order notes if you need a particular format. If you've already received the plate, check out the plate layout. The plate is labelled "Boolean Genetic Logic."

 

Genes

Gene Name Freegenes ID
AND-gate-V2.0 AND-gate-V2.0 BBF10K_000497
OR gate OR gate BBF10K_000498
NOR gate-V2.0 NOR gate-V2.0 BBF10K_000499
XOR gate-V2.0 XOR gate-V2.0 BBF10K_000500
Dual-recombinase-controller Dual-recombinase-controller BBF10K_000501

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Bionet

The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.

Name Contact Country
Ery Fukushima ery.fukushima {at} ikiam {dot} edu {dot} ec Ecuador
Majed Alghamdi mafa2 {at} le {dot} ac {dot} uk United Kingdom
Ian Cubit Djcubit {at} nycap {dot} are {dot} com United States
Cauã Westman caua.westmann {at} ieu {dot} uzh {dot} ch Switzerland
TING-WEI LIN weitinglin66 {at} gmail {dot} com Taiwan
Jordan Gonzalez jgonzalez {at} thecitizensciencelab {dot} org United States

Download all of this information as a CSV from our GitHub.
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