Open Enzymes (Part 2)

Open Enzymes (Part 2)

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The Open Enzyme Collection (Part 2) builds on the earlier collection of essential molecular biology enzymes in OE1 by expanding to include more specialized tools that are less common but still very important in core molecular biology techniques such as restriction-ligation and DNA assembly, genome engineering in bacteria, advanced cloning techniques, amplification of difficult sequences and more. 

For example, it contains restriction enzymes that are used in assembly of DNA parts by many synthetic biology labs (such as GoldenGate and BioBricks type assemblies). It also contains enzymes for high fidelity isothermal and PCR-based amplification of DNA, cutting back and sticking together DNA, making dNTPs (the building blocks of DNA), fixing damaged and degraded DNA prior to amplification and cutting protein sequences to remove unwanted parts. Additionally, it also contains DNA-binding proteins that can be fused with other enzymes to improve their functionality.

What can the collection be used for? 
Researchers who need extended functionality for specialized molecular biology techniques can use the collection as the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice and then transforming into E. coli bacteria. This can help reduce lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder to obtain. The enzymes can also be used as a base to improve functionality or add new functions through genetic engineering and directed evolution.

Details can be found here.


Genes

Eco31I Eco31IA Eco31IB SapI M1.SapI M2.SapI XbaI M.XbaI NotI (GC-rich recognition site) M.NotI SfiI (used to create cDNA libraries) M.SfiI EcoRV M.EcoRV NcoI M.NcoI HindIII M.HindIII DpnI Bst HF ETSSB T5 Exonuclease Tth DNA Ligase K294R dNK from drosophila TEV protease T4 PNK T4-BGT T7EI (T7 Endonuclease I) T4 PGD (T4 Endonuclease V) T4 g32 P45 (ArchaeMaxx/PfuTurbo) Bovine DNTT DNA Topoisomerase 1B Vaccinia Virus lambda red operon UDG Fpg Endonuclease IV E. coli Endonuclease VIII E. coli
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