Saccharomyces cerevisiae (brewers yeast) is one of the most useful microorganisms to humans with usage in brewing beer, making wine, and baking bread. In addition to these traditional uses, this yeast has been instrumental in biotechnology, being one of the primary model organisms used for industrial fermentation of bioengineered metabolic pathways. Currently, there are no free open source toolkits for engineering Saccharomyces cerevisiae, which restricts access to this powerful organism as a platform. The Open Yeast Collection is a free and open DNA toolkit for engineering Saccharomyces cerevisiae, enabling anyone to have a complete and robust toolkit to build new metabolic pathways in this yeast.
What can it be used for:
This first Open Yeast Collection is a foundational and enabling framework for contributing to the creation of an open, sustainable and equitable bioeconomy. More specifically Open Yeast Collection permits the building of plasmids from free, reusable and redistributable genetic parts for genetically modifying Saccharomyces cerevisiae. The plasmids can be used for basic education and research (e.g. protein-protein interactions via yeast-2-hybrid) or for building multi-enzyme metabolic pathways to create a wide variety of natural/commodity chemicals as well as fine and specialized chemicals such as pharmaceuticals. This will appeal to a wide variety of users from educators & students, community-based & academic researchers and bio-entrepreneurs.
Where I can find more information:
- Open Yeast Collection memo
- A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
All genes are present on the plate in E. coli glycerol stocks. Some or all of these items are for use only as permitted by a research exemption.
Genes
| Gene | Name |
|---|---|
| Sc-pAdh1 | S. cerevisiae promoter Adh |
| Sc-pCCW12 | S. cerevisiae promoter CCW12 strong |
| Sc-pCUP1 | S. cerevisiae promoter CUP1 inducible |
| Sc-pCYC1 | S. cerevisiae promoter CYC1 |
| Sc-pGAL1 | S. cerevisiae promoter GAL1 inducible |
| Sc-pHis3 | S. cerevisiae promoter His3 |
| Sc-pLeu2 | S. cerevisiae promoter Leu2 |
| Sc-pMF2 | S. cerevisiae promoter MF2 |
| Sc-pMFA1 | S. cerevisiae promoter MFA1 |
| Sc-pMFalpha2 | S. cerevisiae promoter MFalpha2 |
| Sc-pRPL18B | S. cerevisiae promoter RPL18B |
| Sc-pSAC6 | S. cerevisiae promoter SAC6 |
| Sc-pSte5 | S. cerevisiae promoter Ste5 |
| Sc-pTDH3 | S. cerevisiae promoter TDH3 |
| Sc-pTrp1 | S. cerevisiae promoter Trp1 |
| Sc-pUra3 | S. cerevisiae promoter Ura3 |
| AB_Sc-pCCW12 | S. cerevisiae promoter CCW12 strong (AB enhanced) |
| AB_Sc-pCYC1 | S. cerevisiae promoter CYC1 (AB enhanced) |
| AB_Sc-pGAL1 | S. cerevisiae promoter GAL1 inducible (AB enhanced) |
| AB_Pp-pGAP | Pichia GAP Promoter (enhanced) |
| AB_Pp-pAOX1 | Pichia AOX1 Promoter (enhanced) |
| ScLYS2 | S. cerevisiae cds LYS2 |
| ScMET17 | S. cerevisiae cds MET17 |
| ScAUR1 | S. cerevisiae cds AUR1 |
| Cre | S. cerevisiae cds Cre recombinase |
| FLP | S. cerevisiae cds FLP recombinase |
| GPP synthase | S. cerevisiae cds GPP synthase |
| GGPP synthase | S. cerevisiae cds GGPP synthase |
| FPP synthase | S. cerevisiae cds FPP synthase |
| 4S-limonene-synthase | S. cerevisiae CDS 4S-limonene-synthase |
| limonene-3-hydroxylase | S. cerevisiae cds limonene-3-hydroxylase |
| S-linalool synthase | S. cerevisiae cds LIS (S-linalool synthase) |
| carveol dehydrogenase | S. cerevisiae cds carveol dehydrogenase |
| R-limonene synthase | S. cerevisiae cds R-limonene synthase |
| ScGal4-DNA-AD | S. cerevisiae cds-Gal4-DNA-Activation-Domain |
| ScGal4-DNA-BD | S. cerevisiae cds Gal4 DNA-Binding-Domain |
| N1N2_SV40_NLS | N1N2 SV40 Nuclear Localization Signal (NLS) |
| Pp_aMF_sec_tag | Pichia aMF Secretion Tag |
| Pp_aMF_noEAEA_sec_tag | Pichia aMF_noEAEA secretion tag |
| Sc-tADH1 | S. cerevisiae terminator ADH1 |
| Sc-tUBX6 | S. cerevisiae terminator UBX6 |
| Sc-tENO1 | S. cerevisiae terminator ENO1 |
| Sc-tENO2 | S. cerevisiae terminator ENO2 |
| Sc-tPRM9 | S. cerevisiae terminator PRM9 |
| Sc-tSSA1 | S. cerevisiae terminator SSA1 |
| Sc-tTDH1 | S. cerevisiae terminator TDH1 |
| Pp-tAOX1 | Pichia terminator AOX1 |
| AConL-start-rev | assembly connector left start-rev |
| AConL-start | assembly connector left start |
| AConL1 | assembly connector left 1 |
| AConL2 | assembly connector left 2 |
| AConL3 | assembly connector left 3 |
| AConL4 | assembly connector left 4 |
| AConL5 | assembly connector left 5 |
| AConR1 | assembly connector right 1 |
| AConR2 | assembly connector right 2 |
| AConR3 | assembly connector right 3 |
| AConR4 | assembly connector right 4 |
| AConR5 | assembly connector right 5 |
| AConR-end | assembly connector right end |
| AConR-end-rev | assembly connector right end-rev |
| ScHR5'-LEU2 | S. cerevisiae target 5'-Homology Region LEU2 |
| ScHR3'-LEU2 | S. cerevisiae target LEU2 3'-Homology Region |
| ScHR5'-URA3 | S. cerevisiae target 5'-Homology Region URA3 |
| ScHR3'-URA3 | S. cerevisiae target URA3 3'-Homology Region |
| ScHR5'-HO | S. cerevisiae target 5'-Homology Region HO |
| ScHR3'-HO | S. cerevisiae target HO 3'-Homology Region |
| PpHR5'-NTS | Pichia NTS 3'-Homology Region |
| PpHR3'-NTS | Pichia NTS 5'-Homology Region |
| PpHR5'-RGI2 | Pichia RGI2 3'-Homology Region |
| PpHR3'-RGI2 | Pichia RGI2 5'-Homology Region |
| PpHR5'-ENO | Pichia ENO 3'-Homology Region |
| PpHR3'-ENO | Pichia ENO 5'-Homology Region |
| PpHR5'-AOXTT | Pichia AOXTT 3'-Homology Region |
| PpHR3'-AOXTT | Pichia AOXTT 5'-Homology Region |
| Sc2micron | S. cerevisiae target ori 2micron origin |
| ScARS_CEN | S. cerevisiae target ori ARS_CEN |
Bionet
The bionet enables open peer-peer exchange of functional biomaterials and associated data. This product may also be available from bionet nodes that are more convenient to you. Here are other bionet nodes who may be willing to provide you this specific product.At the moment we are not aware of any other bionet nodes that provide this specific product.